Uninjured adult planarians of the indicated species were pooled and homogenized in 600 ml of TRIzol Reagent (Ambion/Life Technologies). RNA isolation for each species was performed as indicated in the reagent manual. Briefly, phase separation using chloroform was performed, followed by RNA precipitation using isopropanol. The RNA pellets were washed with 75% ethanol and resuspended in 100 ml nuclease-free water to an approximate concentration of 550 ng/ml (P. gracilis), 491 ng/ml (Girardia sp.), 1566 ng/ml (S. mediterranea, asexual), and 764 ng/ml (S. mediterranea, sexual). mRNAseq libraries were generat... [more]
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- Girardia sp. is a common species, found in warm ponds, lakes, and rivers across North America.
- The full grown animals are on average 6-18mm long and 1-3mm wide.
- The head is triangular with a rounded anterior tip.
- The auricles are short and broad.
- The coloration on the dorsal side can vary and often times is almost uniformly brown with irregularly dispersed white splotches.
- The ventral side is less pigmented than the dorsal side.
- Their reproduction is asexual by fission.
... [more]
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- P. gracilis is distributed in the eastern United States, south of the Delaware River and east of the Missouri River.
- The animal are 8-30mm long and 1.5-6mm wide.
- The head is truncated at the anterior end with a slightly bulging frontal margin.
- The auricles are rounded and protruding laterally at the anterior end of the head.
- The coloration on the dorsal side is dark brown to almost black.
- The ventral side is somewhat lighter than the dorsal side.
- There are multiple pharynges that are extruded outside the worm body thr... [more]
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- P. morgani is an inhabitant of springs and cold creeks of eastern North America from New Brunswick to North Carolina and as far west as Kentucky. They have also been found in El Dorado County, California.
- The animals are approximately 14mm long and 2mm wide.
- The head is rectangular with a truncated anterior end and rounded lateral edges.
- The auricles are flattened along the head.
- The coloration on both the dorsal and ventral sides is white.
- Their reproduction is asexual by fission.
... [more]
- D. dorotocephala se puede conseguir en ríos, lagos y charcos a todo lo largo de América del norte.
- Los animales adultos tienen un tamaño promedio de 30mm de largo y 3.5mm de ancho.
- La cabeza es de aspecto triangular con una punta anterior bien definida.
- Las aurículas son elongadas y puntiagudas. Se extienden lateralmente pero normalmente se matienen elevadas sobre la superficie cuando el animal se está desplazando.
- La pigmentación dorsal está compuesta de manchas blancas, negras y marrones.
- El lado ventral del animal es de mucho menor pigmentación que... [more]
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