piwi-1, Pmo_028279 (mRNA) Phagocata morgani

BLAST of piwi-1 vs. uniprot
Match: gi|90101431|sp|Q2Q5Y9|PIWI1_SCHMD (RecName: Full=Piwi-like protein 1; AltName: Full=SMEDWI-1 [Schmidtea mediterranea])

HSP 1 Score: 1167.91 bits (3020), Expect = 0.000e+0
Identity = 544/746 (72.92%), Postives = 647/746 (86.73%), Query Frame = 1

Query:  199 FFMYDVKMPPKSLRGKLDLLNSVASTKEN-RPFCFDGRRLFTSSQWHQ-EDIETISIKDEEVSIRFLASFVENTEEYYQMVNIIFNNIQAFMGQERIGRAFFLGSNSNESTSKCGPSFFKRDNFKILGGFATTLSRGTNSDGSASTLLYLERINRVLNENSVYNIYNKNQLDLLIGRDVITKYNNKTYRISDIKEMDVNDEIELNGTKISYANYFKQRYNITLNNGKQPFVISRLRRVAIQQKENQENEPEAPKEADQSLSIPGELCFLCGFSDSERSNINLQKSLGSVLKREPRERLKDISDFCSWTDEGKAAPFMKNWGVQLDNQPITIMGRELNGVDVFSGQQRINAKSPDDWKFGEVKFDIPKGAQHRFAILAVERSPSQFNQFIDDVKREISRLRVNYSIETVATCRANEIEDCLTDFVRVQKVHLALVFIGDDKAYARVKNFTISTGLLTQCVTVRNGTNXXXXXXKVVADKSVMQIFAKLGYDPWGIELKMVPTMVVGLDTFHSKTGKRSIQASVFSISSKFSQYLSFVNSPKGKNEYHDNLSQNFIAAINTFKDKFNTLPQRFIIYRDGVGDSQLYFTKKYETDAVLKIMETIYENENLPQIIYIIVKKRISVKFFKDGNNPNPGTIVDEKVVKPNFYEFYLVSQRTTKGTATPTNYNVLMDTQLTNRKTGEKAPIPAGQLQHITYALTHLYFNWMGTIRVPVPTHYAHRLAELVGTVHRGTNPPVISDRIRERLFYL 2430
            F+MYDV M  KS+R KL+LL + A++K N +PFCFDGRRLFT+S+WH  ED E + IKD E+SIR L +F++N+EEYYQM+NI+FNNIQ FMGQE+IGR FFLGSN      + GPSFFK DNFKILGG++TT++RGTN +G+ASTLLYLERINRVLNEN+V ++Y ++Q+D LIGRD+ITKYNNKTYRISDIKEMDVNDE++L   KISY NYFKQRYNI L N KQPFVISR++R  ++ KE  ENEPE P E DQSLSIPGELC+LCGFSDSERSNINLQK+LG+VLKREPRERL DI DFC WT  GK+  +MK+WG+ ++ QPITI GREL  VD+FS + +IN K PDDWKFGE+KFD+PKG  HRF +L V+R+PS FN FI+DVKREI RLR+NY+++++++CR+NE+ED LTDF+RV KVH+ALVFI DDK YA+VKNFT+STGLLTQCVT RNG+NRDDRRRK VADKSVMQ+F+KLGYDPWGI LKM PTM+VGLDTFHSKTGKRS+QASVFSIS+KFSQY+SFVNS KGKNE+H+NL +NF+ A+ TF++KFNT+P R IIYRDGVGDSQL FTKK+ETDAV+K++E IYEN+ LPQIIY++VKKRISVKFFKDG NPNPGT+VDEK+VKPNFYEFYLVSQ+TTKGTA+PTNYNVLMDT+ TN+KT E + +    LQ ITY+LTHLYFNWMGTIRVPVPTHYAHRLAELVG +HRG  PP I+DRIRERLFYL
Sbjct:   63 FYMYDVVMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSDEDTENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSVYRRDQIDQLIGRDIITKYNNKTYRISDIKEMDVNDEVQLGDKKISYLNYFKQRYNINLKNDKQPFVISRVKRSMVRPKEKTENEPEGPTETDQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAGKSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWKFGEIKFDVPKGTAHRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRVSKVHMALVFIPDDKVYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFKDGANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGKIHRGVTPPAINDRIRERLFYL 808          

HSP 2 Score: 54.299 bits (129), Expect = 4.217e-6
Identity = 34/50 (68.00%), Postives = 44/50 (88.00%), Query Frame = 3

Query:   54 KRGGLQKRFYIIEPSLQPXXXXXXXXXXXXXXXLKSNYANFKLAMKGPFF 203
            +RGGLQ+RF IIEP+LQPD V DK+GKDG+ V+LKSNYANF + ++GPF+
Sbjct:   15 RRGGLQRRFAIIEPALQPDTVTDKIGKDGRVVSLKSNYANFNIKVEGPFY 64          

BLAST of piwi-1 vs. uniprot
Match: gi|90101433|sp|Q2PC95|PIWIL_DUGJA (RecName: Full=Piwi-like protein [Dugesia japonica])

HSP 1 Score: 891.338 bits (2302), Expect = 0.000e+0
Identity = 434/755 (57.48%), Postives = 563/755 (74.57%), Query Frame = 1

Query:  199 FFMYDVKMPPKSLRGKLDLLNSVASTKE-NRP--FCFDGRRLFTSSQWHQ-EDIETISIKDEEVSIRFLASFVENTEEYYQMVNIIFNNIQAFMGQERIGRAFFLGSNSNES--TSKCGPSFFKRDNFKILGGFATTLSRGTNSDGSASTLLYLERINRVLNENSVYNIYNKNQLDLLIGRDVITKYNNKTYRISDIKEMDVNDEIELNGTKISYANYFKQRYNITLNNGKQPFVISRLRRVAIQQKENQENEPEAPKEADQSLSIPGELCFLCGFSDSERSNINLQKSLGSVLKREPRERLKDISDFCSWTDEGKAAPFMKN-WGVQLDNQPITIMGRELNGVDVFSGQQRINAKSPDDWKFGEVKFDIPKGAQHRFAILAVERSPSQFNQFIDDVKREISRLRVNYSIETVATCRANEIEDCLTDFVRVQK--VHLALVFIGDDKAYARVKNFTISTGLLTQCVTVRNGTNXXXXXXKVVADKSVMQIFAKLGYDPWGIELKMVPTMVVGLDTFHSKTGKRSIQASVFSISSKFSQYLSFVNSPKGKNEYHDNLSQNFIAAINTFKDKFNTLPQRFIIYRDGVGDSQLYFTKKYETDAVLKIMETIYENEN--LPQIIYIIVKKRISVKFFKDGNNPNPGTIVDEKVVKPNFYEFYLVSQRTTKGTATPTNYNVLMDTQLTNRKTGEKAPIPAGQLQHITYALTHLYFNWMGTIRVPVPTHYAHRLAELVGTVHRGTNPPVISDRIRERLFYL 2430
            + MYD       L  KL L   V   KE N P  FC+DGRRLFTS  W +  DI+  +I +++VS+R +++F+ +TEEYYQM+N++ NN+Q  MGQE+IG+ +FLGSNSN+       GP+FF+ +NFK+LGGF TTL RGT  +G  +TLLY+ERINRVLN+NSV   YN+  +D LIGRD++TKYNNKTYRIS+IKEM++N+  E+ G  +SYA YFK+RYNI L   +QPFV++R++++A + K  + NE EAP++ D +L+I GELCFLCGFSD E+SNI+LQK+LG VLKR PRERL DI  FC W      +  M N WG+++DN P+ I GREL   DV SG QRIN K  DDWKFG+V+F++ KG +H   ++ V+R+ SQ+  F++DV+ EI +LR +  I  V  C AN++E  L D +R       +ALVF+ DD+ YA+VK+FTISTGLLTQCVT RNGTNR+D+RR+VV+ K+VMQIF+K GYDPW I++KM PTM+VG+DTFH+K  K+SI ASVFSI+S FSQY+SF NSPKGK E+HD LS NF AA+  FK  +  LP R ++YRDGVGDSQL FTK++E DA+  ++E IY+     +PQIIY+IVKKRI  K F  G+N NPGTIVD+++VKPNF+EF+LVSQR T+GTA+PTNYNVL DT+  N K G    I   +LQ ITY LTHLYFNWMGTIRVPVP HYAH+LAEL+G VH+G+ P +I++RIR RLFYL
Sbjct:   65 YIMYDTTFSKMGLSPKLKLQILVRICKEYNLPGSFCYDGRRLFTSDNWTEGSDIKEFTIDEKKVSLRLVSTFLSDTEEYYQMINVLLNNLQIIMGQEKIGKGYFLGSNSNKDNVVKNIGPTFFETNNFKVLGGFGTTLQRGTRPNGQLTTLLYIERINRVLNDNSVLVTYNRKNIDQLIGRDILTKYNNKTYRISEIKEMNINETFEMVGRTLSYAQYFKERYNINLTTEQQPFVLTRVKKIARRDKRKEHNENEAPEDMDLTLNITGELCFLCGFSDQEKSNIDLQKNLGCVLKRGPRERLDDIPSFCDWIKTNNNSQGMSNKWGLKIDNTPLEIEGRELPHCDVISGGQRINEKIKDDWKFGKVQFNVQKGVKHEIDVVIVDRNDSQYGNFLNDVEYEIKQLRFDAKIGRVLVCSANDVERSLNDVMRSGSGCAKIALVFVPDDRVYAKVKSFTISTGLLTQCVTTRNGTNRNDKRRQVVSSKTVMQIFSKFGYDPWTIDIKMRPTMIVGMDTFHNKGSKKSIHASVFSINSTFSQYMSFANSPKGKQEFHDTLSGNFKAALTEFKRIYKILPVRIMVYRDGVGDSQLQFTKQFEVDAMKPLVENIYKENGCQVPQIIYVIVKKRIGTKLFNRGDNANPGTIVDKEIVKPNFFEFFLVSQRVTRGTASPTNYNVLEDTRYQN-KAGIIEAITPNELQRITYTLTHLYFNWMGTIRVPVPVHYAHKLAELIGKVHKGSTPALINERIRNRLFYL 818          

BLAST of piwi-1 vs. uniprot
Match: gi|90101432|sp|Q2Q5Y8|PIWI2_SCHMD (RecName: Full=Piwi-like protein 2; AltName: Full=SMEDWI-2 [Schmidtea mediterranea])

HSP 1 Score: 868.996 bits (2244), Expect = 0.000e+0
Identity = 428/763 (56.09%), Postives = 568/763 (74.44%), Query Frame = 1

Query:  199 FFMYDVKMPPKSLRGKLDLLNSVASTKENR---PFCFDGRRLFTSSQWHQE-DIETISIKDEEVSIRFLASFVENTEEYYQMVNIIFNNIQAFMGQERIGRAFFLGSN--SNESTSKCGPSFFKRDNFKILGGFATTLSRGTNSDGSASTLLYLERINRVLNENSVYNIYNKNQLDLLIGRDVITKYNNKTYRISDIKEMDVNDEIELNGTKISYANYFKQRYNITLNNGKQPFVISRLRRVAIQQK-------ENQENEPEAPKEADQSLSIPGELCFLCGFSDSERSNINLQKSLGSVLKREPRERLKDISDFCSWTDEGKAAPFMKN-WGVQLDNQPITIMGRELNGVDVFSGQQRINAKSPDDWKFGEVKFDIPKGAQHRFAILAVERSPSQFNQFIDDVKREISRLRVNYSIETVATCRANEIEDCLTDFVRVQK--VHLALVFIGDDKAYARVKNFTISTGLLTQCVTVRNGTNXXXXXXKVVADKSVMQIFAKLGYDPWGIELKMVPTMVVGLDTFHSKTGKRSIQASVFSISSKFSQYLSF-VNSPKGKNEYHDNLSQNFIAAINTFKDKFNTLPQRFIIYRDGVGDSQLYFTKKYETDAVLKIMETIYENEN--LPQIIYIIVKKRISVKFFKDGNNPNPGTIVDEKVVKPNFYEFYLVSQRTTKGTATPTNYNVLMDTQLTNRKTGEKAPIPAGQLQHITYALTHLYFNWMGTIRVPVPTHYAHRLAELVGTVHRGTNPPVISDRIRERLFYL 2430
            F MYD       L  K+ L   V   +EN+    FCFDGRRLFTS +WH E DI+  S  ++++++R +++ +  TEEYYQM+N++ NN+Q  +GQERIG+ +FL  N   +E     GP+FF+ ++FK+LGGF TTL RGT   G  +TLLY+ERINRVLN+NSV   YN+  +D LIGRD+ITKYNNKTYRIS+IKEM+V+++ E+ G  +SYA YFK+RYNI L  G QPFV++R+++   +++         +E E EAP+E D +L+IPGELCFLCGFSD E+SN++LQK+LG VLKREPRERL DI  +C+W     AA  M N W +++DN+P+ I GREL   DV SG  +IN K  DDWKFG V+FDI +  +H   ++ V+R+  Q+  F++DV++E+  +R++  +  V TC  N++E CL D  R       +ALVF+ DD+ YA+VK+FT+STGLLTQCVT RNGTNR+D+RRKVV+ K+VMQIFAK GYDPW +E+K+ PTM+VG+DT+H+K+ K+SIQASVFSI+S F+QY+SF VNSPKG+ E+H+ L +NF  A+  FK +++ LPQR +++RDGVGD+QL FTK +E DA+  ++E IY+     +PQIIY+IVKKR+  K F  GNNPNPGT+VD+++VKPNFYEF+LVSQRTTKGTATPTNYNVL DT+L  +K G   P+   +LQ ITYALTHLYFNWMGTIRVPVP HYAHRLAELVG VH+G+NP  I++RIR RLFYL
Sbjct:   72 FMMYDSVFSQVGLSPKVKLQFIVRVCQENKLPGAFCFDGRRLFTSEKWHGESDIQEFSHDEKKMTLRLVSTILPETEEYYQMINVLLNNLQVMLGQERIGKGYFLSPNITKDEVPRNSGPTFFETNSFKVLGGFGTTLQRGTKPTGELTTLLYIERINRVLNDNSVMKAYNRRSIDQLIGRDIITKYNNKTYRISEIKEMNVDEKFEMGGRTLSYAEYFKERYNIRLTQGDQPFVLTRVKKPMRRERKKKDEEGVEKEKEKEAPEEKDMTLNIPGELCFLCGFSDQEKSNMDLQKNLGCVLKREPRERLDDIPAYCNWIKNSDAATGMCNKWQLKIDNKPLEIEGRELPPCDVISGGSKINEKMGDDWKFGRVQFDIKRDRKHEIDVVIVDRNDFQYKNFMNDVEQELRNMRIDARVGKVNTCGPNDVERCLNDAARSGSGCAKMALVFVPDDRVYAKVKSFTMSTGLLTQCVTTRNGTNRNDKRRKVVSSKTVMQIFAKFGYDPWTVEIKLRPTMIVGMDTYHNKSSKKSIQASVFSINSTFTQYMSFVVNSPKGRQEFHETLGKNFNLALEDFKKRYDILPQRILVFRDGVGDNQLQFTKNFEVDAMKPLIENIYKGNGFPVPQIIYVIVKKRVGTKLFNRGNNPNPGTVVDKEIVKPNFYEFFLVSQRTTKGTATPTNYNVLEDTRLLTKK-GTMDPMAPNELQKITYALTHLYFNWMGTIRVPVPVHYAHRLAELVGKVHKGSNPVAINERIRNRLFYL 833          

BLAST of piwi-1 vs. uniprot
Match: gi|33112431|sp|Q9VKM1|PIWI_DROME (RecName: Full=Protein piwi)

HSP 1 Score: 261.151 bits (666), Expect = 8.390e-73
Identity = 201/732 (27.46%), Postives = 343/732 (46.86%), Query Frame = 1

Query:  292 FCFDGRRLFTSSQWHQEDIETISIKDE-----EVSIRFLASFVENTEEYYQMVNIIFNNIQAFMGQERIGRAFFLGSNSNESTSKCGPSFFKRDNFKILGGFATTLSRGTNSDGSASTLLYLERINRVLNENSVYNIYNK--------------NQLDLLIGRDVITKYNNKTYRISDIK-EMDVNDEIELNGTKISYANYFKQRYNITLNNGKQPFVISRLRRVAIQQKENQENEPEAPKEADQSLSIPGELCFLCGFSDSERSNINLQKSLGSVLKREPRERLKDISDF-CSWTDEGKAAPFMKNWGVQLDNQPITIMGRELNGVDVFSGQQRINAKSPDDWK--FGEVK-FDIPKGAQHRFAILAVERSPSQFNQFIDDVKREIS----RLR-----VNYSIETVATCRANEIEDCLTDFVRVQKVHLALVFIGDDKA--YARVKNFT-ISTGLLTQCVTVRNGTNXXXXXXKVVADKSVMQIFAKLGYDPWGIELKMVPTMVVGLDTFHS-KTGKRSIQASVFSIS-SKFSQYLSFVNSPKGKNEYHDNLSQNFIAAINTFKDKFNTLPQRFIIYRDGVGDSQLYFTKKYETDAVLKIMETIYENENL--PQIIYIIVKKRISVKFFKDGNNPNPGTIVDEKVVKPNFYEFYLVSQRTTKGTATPTNYNVLMDTQLTNRKTGEKAPIPAGQLQHITYALTHLYFNWMGTIRVPVPTHYAHRLAELVGT 2367
            + FDG +LFT+ ++ QE I  +S K +     ++SI+F+         + Q++N+I       +  E +GR  F      +  +K     FK    ++  G+ T++ +          LL  E  ++V+   ++Y+I  +              N LDL+    V+T YNN+TYRI+D+             G  IS+  Y+  +YNI + +  QP +IS+ R  A++              A + + +  ELC + G +   RSN  L +++ S  +  P++R   +  F     +  ++   +++W ++LD     + GR +   ++     ++ A    DW+  F + +    P     R+A++A +R+  +    +D + R  S    R+R     + Y   T    RA  ++DC+          L L  + +D A  Y+ +K    +   + TQ VT++   NR       +A K  +Q+  KLGY PW IEL +   M +G D   S +  KR+  A + S+   + S Y S V      +   + L      A+  ++ +   LP R + YRDGV    L    ++E   +++ ++T Y    L  PQ+ YI+V + ++ +FF +G NP PGTIVD+ +  P  Y+FYLVSQ+  +GT +PT+YNVL  +            +   ++Q +TY + HLY+NW GT RVP    YA +LA LVGT
Sbjct:  146 YLFDGLQLFTTRKFEQE-ITVLSGKSKLDIEYKISIKFVGFISCAEPRFLQVLNLILRRSMKGLNLELVGRNLF------DPRAKIEIREFK---MELWPGYETSIRQH-----EKDILLGTEITHKVMRTETIYDIMRRCSHNPARHQDEVRVNVLDLI----VLTDYNNRTYRINDVDFGQTPKSTFSCKGRDISFVEYYLTKYNIRIRDHNQPLLISKNRDKALK------------TNASELVVLIPELCRVTGLNAEMRSNFQLMRAMSSYTRMNPKQRTDRLRAFNHRLQNTPESVKVLRDWNMELDKNVTEVQGRIIGQQNIVFHNGKVPAGENADWQRHFRDQRMLTTPSDGLDRWAVIAPQRNSHELRTLLDSLYRAASGMGLRIRSPQEFIIYDDRTGTYVRA--MDDCVRS-----DPKLILCLVPNDNAERYSSIKKRGYVDRAVPTQVVTLKTTKNRSLMS---IATKIAIQLNCKLGYTPWMIELPLSGLMTIGFDIAKSTRDRKRAYGALIASMDLQQNSTYFSTVTECSAFDVLANTLWPMIAKALRQYQHEHRKLPSRIVFYRDGVSSGSLKQLFEFEVKDIIEKLKTEYARVQLSPPQLAYIVVTRSMNTRFFLNGQNPPPGTIVDDVITLPERYDFYLVSQQVRQGTVSPTSYNVLYSSM----------GLSPEKMQKLTYKMCHLYYNWSGTTRVPAVCQYAKKLATLVGT 826          

BLAST of piwi-1 vs. uniprot
Match: gi|226701015|sp|A6N7Y9|PIWL1_CHICK (RecName: Full=Piwi-like protein 1)

HSP 1 Score: 260.766 bits (665), Expect = 1.261e-72
Identity = 207/749 (27.64%), Postives = 348/749 (46.46%), Query Frame = 1

Query:  298 FDGRRLFTSSQWHQEDIETISI----KDEEVSIRFLASFVENTEEYYQMVNIIFNNIQAFMGQERIGRAFFLGSNSNESTSKCGPSFFKRDNFKILGGFATTLSRGTNSDGSASTLLYLERINRVLNENSV----YNIYNKNQ--------LDLLIGRDVITKYNNKTYRISDIKEMDVNDEIEL---NGTKISYANYFKQRYNITLNNGKQPFVISRLRRVAIQQKENQENEPEAPKEADQSLSIPGELCFLCGFSDSERSNINLQKSLGSVLKREPRERLKDISDF--CSWTDEGKAAPFMKNWGVQLDNQPITIMGRELNGVDVFSGQQRI--NAKSPDDWKFGEVKFDIPKGAQHRFAILAVERSPSQFNQFIDDVKREISRLRVNYSIETVATCRANEIEDCLTDFVRVQK---------VHLALVFIGDDKAYARVKNFTISTGLLTQCVTVRNGTNXXXXXXKVVADKSVMQIFAKLGYDPWGIELKMVPTMVVGLDTFH-SKTGKRSIQASVFSISSKFSQYLSFVNSPKGKNEYHDNLSQNFIAAINTFKDKFNTLPQRFIIYRDGVGDSQLYFTKKYETDAVLKIMETIYENENLPQIIYIIVKKRISVKFFKDGN----NPNPGTIVDEKVVKPNFYEFYLVSQRTTKGTATPTNYNVLMDTQLTNRKTGEKAPIPAGQLQHITYALTHLYFNWMGTIRVPVPTHYAHRLAELVG-TVHRGTNPPVISDRIRERLFYL 2430
            FDG  LF   +   +  E  S     +D  ++I         +    Q  NIIF  +   M  ++IGR ++   N  +  S             +  GF +++ +        S +L  +  +++L   +V    Y++Y + +           LIG  V+TKYNN+TYR+ DI + D N +      +G++ISY +Y+K++YN  +++  QP +IS+ RR    ++ N    P         + IP ELC+L G ++  R++ N+ K L    +  P +R ++I     C   DE      +++WG+  D+  ++  GR +    +         N +  D  +   V   I       + ++   R+    N  + ++ +    + +  +  T+      E++D    ++RV +         V   L     DK  A  K       + +QCV  R  T    +    +  K  +Q+  K+G + W +E+ +   M+VG+D +H + +GK+SI   V S++ K +++ S         E  D L      A+  +      LP R I+YRDGVGD QL     YE    L  ++T+ ++ N P++  I+VKKR+S +FF        NP PGT+VD +V +P +Y+F++VSQ    G   PT+YNV+ DT          + +    +Q +TY L H+Y+NW G IRVP P  YAH+LA LVG ++HR  N  ++SD    RL+YL
Sbjct:  171 FDGSILFLPKRLPNKVTEVYSKTRNGEDVRITITLTNELPPTSPTCLQFYNIIFRRLLKMMNFQQIGRNYY---NPKDPVS------IPNHRLMVWPGFTSSILQY-----EESIMLCADVSHKILRSETVLDFMYSLYEQVEERRFRDACAKELIGVIVLTKYNNRTYRVDDI-DWDANPQCTFRRADGSEISYIDYYKRQYNQDISDLNQPVLISQYRR----KRGNVTVGP--------VVLIP-ELCYLTGLTEKMRNDFNMMKDLAVHTRLSPEQRQREIGKLVDCMKKDEC-VQKELRDWGLSFDSSLLSFTGRVVQAEKILQAGNVFDYNPQFADWSRETRVAPLIHAKPLDNWLLIYTRRNYDAANMLLQNLFKVTPSMGIRMNKATMI-----EVDDRTEAYLRVLQQSITPDTNIVVCILSSTRKDKYDAIKKYLCTDCPIPSQCVVAR--TLSKPQTALAIVTKIALQMNCKMGGELWSVEIPLKQLMIVGIDCYHDTLSGKQSIAGFVASLNEKMTRWFSRCVVQSRGQEIVDGLKACLQTALREWFKWNKYLPSRIIVYRDGVGDGQLNTLVNYEVPQFLDCLKTVGKDYN-PRLTVIVVKKRVSTRFFAQAGGGLKNPPPGTVVDIEVTRPEWYDFFIVSQAVRNGCVAPTHYNVVYDT----------SKLKPDHVQRLTYKLCHMYYNWSGVIRVPAPCQYAHKLAFLVGQSIHREPN-LLLSD----RLYYL 867          

BLAST of piwi-1 vs. Smed Unigenes AA
Match: SMU15026177 (SmedSxldir_c80243_g1_i2|m.21809|m.41374)

HSP 1 Score: 1167.53 bits (3019), Expect = 0.000e+0
Identity = 545/747 (72.96%), Postives = 648/747 (86.75%), Query Frame = 1

Query:  199 FFMYDVKMPPKSLRGKLDLLNSVASTKEN-RPFCFDGRRLFTSSQWHQ-EDIETISIKDEEVSIRFLASFVENTEEYYQMVNIIFNNIQAFMGQERIGRAFFLGSNSNESTSKCGPSFFKRDNFKILGGFATTLSRGTNSDGSASTLLYLERINRVLNENSVYNIYNKNQLDLLIGRDVITKYNNKTYRISDIKEMDVNDEIELNGTKISYANYFKQRYNITLNNGKQPFVISRLRRVAIQQKENQENEPEAPKEADQSLSIPGELCFLCGFSDSERSNINLQKSLGSVLKREPRERLKDISDFCSWTDEGKAAPFMKNWGVQLDNQPITIMGRELNGVDVFSGQQRINAKSPDDWKFGEVKFDIPKGAQHRFAILAVERSPSQFNQFIDDVKREISRLRVNYSIETVATCRANEIEDCLTDFVRVQKVHLALVFIGDDKAYARVKNFTISTGLLTQCVTVRNGTNXXXXXXKVVADKSVMQIFAKLGYDPWGIELKMVPTMVVGLDTFHSKTGKRSIQASVFSISSKFSQYLSFVNSPKGKNEYHDNLSQNFIAAINTFKDKFNTLPQRFIIYRDGVGDSQLYFTKKYETDAVLKIMETIYENENLPQIIYIIVKKRISVKFFKDGNNPNPGTIVDEKVVKPNFYEFYLVSQRTTKGTATPTNYNVLMDTQLTNRKTGEKAPIPAGQLQHITYALTHLYFNWMGTIRVPVPTHYAHRLAELVGTVHRGTNPPVISDRIRERLFYL* 2433
            F+MYDV M  KS+R KL+LL + A++K N +PFCFDGRRLFT+S+WH  ED E + IKD E+SIR L +F++N+EEYYQM+NI+FNNIQ FMGQE+IGR FFLGSN      + GPSFFK DNFKILGG++TT++RGTN +G+ASTLLYLERINRVLNEN+V ++Y ++Q+D LIGRD+ITKYNNKTYRISDIKEMDVNDE++L   KISY NYFKQRYNI L N KQPFVISR++R  ++ KE  ENEPE P E DQSLSIPGELC+LCGFSDSERSNINLQK+LG+VLKREPRERL DI DFC WT  GK+  +MK+WG+ ++ QPITI GREL  VD+FS + +IN K PDDWKFGE+KFD+PKG  HRF +L V+R+PS FN FI+DVKREI RLR+NY+++++++CR+NE+ED LTDF+RV KVH+ALVFI DDK YA+VKNFT+STGLLTQCVT RNG+NRDDRRRK VADKSVMQ+F+KLGYDPWGI LKM PTM+VGLDTFHSKTGKRS+QASVFSIS+KFSQY+SFVNS KGKNE+H+NL +NF+ A+ TF++KFNT+P R IIYRDGVGDSQL FTKK+ETDAV+K++E IYEN+ LPQIIY++VKKRISVKFFKDG NPNPGT+VDEK+VKPNFYEFYLVSQ+TTKGTA+PTNYNVLMDT+ TN+KT E + +    LQ ITY+LTHLYFNWMGTIRVPVPTHYAHRLAELVG +HRG  PP I+DRIRERLFYL*
Sbjct:  106 FYMYDVVMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSDEDTENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSVYRRDQIDQLIGRDIITKYNNKTYRISDIKEMDVNDEVQLGDKKISYLNYFKQRYNINLKNDKQPFVISRVKRSMVRPKEKTENEPEGPTETDQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAGKSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWKFGEIKFDVPKGTAHRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRVSKVHMALVFIPDDKVYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFKDGANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGKIHRGVTPPAINDRIRERLFYL* 852          

HSP 2 Score: 53.9138 bits (128), Expect = 3.909e-7
Identity = 34/50 (68.00%), Postives = 44/50 (88.00%), Query Frame = 3

Query:   54 KRGGLQKRFYIIEPSLQPXXXXXXXXXXXXXXXLKSNYANFKLAMKGPFF 203
            +RGGLQ+RF IIEP+LQPD V DK+GKDG+ V+LKSNYANF + ++GPF+
Sbjct:   58 RRGGLQRRFAIIEPALQPDTVTDKIGKDGRVVSLKSNYANFNIKVEGPFY 107          

BLAST of piwi-1 vs. Smed Unigenes AA
Match: SMU15015947 (SmedSxldir_c101456_g1_i1|m.49141|m.12333)

HSP 1 Score: 872.463 bits (2253), Expect = 0.000e+0
Identity = 424/767 (55.28%), Postives = 567/767 (73.92%), Query Frame = 1

Query:  199 FFMYDVKMPPKSLRGKLDLLNSVASTKENR---PFCFDGRRLFTSSQWHQE-DIETISIKDEEVSIRFLASFVENTEEYYQMVNIIFNNIQAFMGQERIGRAFFLGSN--SNESTSKCGPSFFKRDNFKILGGFATTLSRGTNSDGSASTLLYLERINRVLNENSVYNIYNKNQLDLLIGRDVITKYNNKTYRISDIKEMDVNDEIELNGTKISYANYFKQRYNITLNNGKQPFVISRLRRVAIQQKENQENEPEAPKEA-----------DQSLSIPGELCFLCGFSDSERSNINLQKSLGSVLKREPRERLKDISDFCSWTDEGKAAPFMKN-WGVQLDNQPITIMGRELNGVDVFSGQQRINAKSPDDWKFGEVKFDIPKGAQHRFAILAVERSPSQFNQFIDDVKREISRLRVNYSIETVATCRANEIEDCLTDFVRVQK--VHLALVFIGDDKAYARVKNFTISTGLLTQCVTVRNGTNXXXXXXKVVADKSVMQIFAKLGYDPWGIELKMVPTMVVGLDTFHSKTGKRSIQASVFSISSKFSQYLSFVNSPKGKNEYHDNLSQNFIAAINTFKDKFNTLPQRFIIYRDGVGDSQLYFTKKYETDAVLKIMETIYENEN--LPQIIYIIVKKRISVKFFKDGNNPNPGTIVDEKVVKPNFYEFYLVSQRTTKGTATPTNYNVLMDTQLTNRKTGEKAPIPAGQLQHITYALTHLYFNWMGTIRVPVPTHYAHRLAELVGTVHRGTNPPVISDRIRERLFYL* 2433
            F MYD       L  K+ L   V   +EN+    FCFDGRRLFTS +WH E DI+  S  ++++++R +++ +  TEEYYQM+N++ NN+Q  +GQERIG+ +FL  N   +E     GP+FF+ ++FK+LGGF TTL RGT   G  +TLLY+ERINRVLN+NSV   YN+  +D LIGRD+ITKYNNKTYRIS+IKEM+V+++ E+ G  +SYA YFK+RYNI L  G QPFV++R+++   ++++ ++ E    ++            D +L+IPGELCFLCGFSD E+SN++LQK+LG VLKREPRERL DI  +C+W     AA  M N W +++DN+P+ I GREL   DV SG  +IN K  DDWKFG V+FDI +  +H   ++ V+R+  Q+  F++DV++E+  +R++  +  V TC  N++E CL D  R       +ALVF+ DD+ YA+VK+FT+STGLLTQCVT RNGTNR+D+RRKVV+ K+VMQIFAK GYDPW +E+K+ PTM+VG+DT+H+K+ K+SIQASVFSI+S F+QY+SFVNSPKG+ E+H+ L +NF  A+  FK +++ LPQR +++RDGVGD+QL FTK +E DA+  ++E IY+     +PQIIY+IVKKR+  K F  GNNPNPGT+VD+++VKPNFYEF+LVSQRTTKGTATPTNYNVL DT+L  +K G   P+   +LQ ITYALTHLYFNWMGTIRVPVP HYAHRLAELVG VH+G+NP  I++RIR RLFYL*
Sbjct:   72 FMMYDSVFSQVGLSPKVKLQFIVRVCQENKLPGAFCFDGRRLFTSEKWHGESDIQEFSHDEKKMTLRLVSTILPETEEYYQMINVLLNNLQVMLGQERIGKGYFLSPNITKDEVPRNSGPTFFETNSFKVLGGFGTTLQRGTKPTGELTTLLYIERINRVLNDNSVMKAYNRRSIDQLIGRDIITKYNNKTYRISEIKEMNVDEKFEMGGRTLSYAEYFKERYNIRLTQGDQPFVLTRVKKPMRRERKKKDEEGVEKEKEKEREKEAPEEKDMTLNIPGELCFLCGFSDQEKSNMDLQKNLGCVLKREPRERLDDIPAYCNWIKNSDAATGMCNKWQLKIDNKPLEIEGRELPPCDVISGGSKINEKMGDDWKFGRVQFDIKRDRKHEIDVVIVDRNDFQYKNFMNDVEQELRNMRIDARVGKVNTCGPNDVERCLNDAARSGSGCAKMALVFVPDDRVYAKVKSFTMSTGLLTQCVTTRNGTNRNDKRRKVVSSKTVMQIFAKFGYDPWTVEIKLRPTMIVGMDTYHNKSSKKSIQASVFSINSTFTQYMSFVNSPKGRQEFHETLGKNFNLALEDFKKRYDILPQRILVFRDGVGDNQLQFTKNFEVDAMKPLIENIYKGNGFPVPQIIYVIVKKRVGTKLFNRGNNPNPGTVVDKEIVKPNFYEFFLVSQRTTKGTATPTNYNVLEDTRLLTKK-GTMDPMAPNELQKITYALTHLYFNWMGTIRVPVPVHYAHRLAELVGKVHKGSNPVAINERIRNRLFYL* 837          

BLAST of piwi-1 vs. Smed Unigenes AA
Match: SMU15000111 (Asxlregen_comp31087_c0_seq1|m.709|m.20657)

HSP 1 Score: 248.054 bits (632), Expect = 4.994e-69
Identity = 199/712 (27.95%), Postives = 341/712 (47.89%), Query Frame = 1

Query:  409 NTEEYYQMVNIIFNNIQAFMGQERIGRAFFLGSNSNESTSKCGPSFFKRDNFKILGGFATTLSRGTNSDGSASTLLYLERINRVLNENSVYNIY--------NKNQL-----DLLIGRDVITKYNNKTYRISDIK-EMDVNDEIELNGTKISYANYFKQRYNITLNNGKQPFVISRLRRVAIQQKENQENEPEAPKEADQSLS--IPGELCFLCGFSDSERSNINLQKSLGSVLKREPRERLKDISDFCS--WTDEGKAAPFMKNWGVQLDNQPITIMGRELNGVDVFSGQQ-RINAKSPDDWKFGEVKFDIPKGAQHRFAILAVERSP-SQFNQFIDDVKREISRLRVNYSIETVATCRANEIEDCLTDFVR-----VQKVHLALVFIGDDKAYARVKNFT-ISTGLLTQCV---TVRNGTNXXXXXXKVVADKSVMQIFAKLGYDPWGIELKMVPTMVVGLDTFHSKTGK-RSIQASVFSISSKFSQYLSFVNSPKGKNEYHDNLSQNFIAAINTFKDKFNTLPQRFIIYRDGVGDSQLYFTKKYETDAVLKIMETIYENENLPQIIYIIVKKRISVKFFKDG-------NNPNPGTIVDEKVVKPNFYEFYLVSQRTTKGTATPTNYNVLMDTQLTNRKTGEKAPIPAGQLQHITYALTHLYFNWMGTIRVPVPTHYAHRLAELVGTVHRGTNPPVISDRIRERLFYL* 2433
            N  E   + N++F  I+  +   +IGR +F                  R   +I  G+ T++    N DG    LL  +  ++V+  +SVY+I         NK Q+     + L+G+ V+T +NN+ YRI+DI    +   E +  G KISY +YFK  Y + + + +QP ++S+        +  +   P+  KE D  L   IP ELC L G +D    +  L + L    +  P++R + + +F    ++ E +A   +  WG+ ++   + I   ++N   ++ G +  ++A    ++K       +  G +    IL   +S  ++ N  +  +  +  R+ V +    +     +  E+ L +  R     VQ V   L  + +D+ Y  +K F  +   + +Q V   T++ G   +      VA    +QI AKLG + W +++ +   MVVGLD +H   G+ RS+ A V S ++  +++ S  +  +   E    L    ++ +  + ++ N LP+   +YRDGV D QL + +K E +   K+ E+ +  +  P ++Y +V+KRI+ + +          NNPNPGTIVD  V + N Y+F+LVSQ   +GT TPT+Y VL D           +     Q+Q + Y   H+Y+NW GT+RVP P  YAH+LA +VG   +     ++ D    RLFYL*
Sbjct:  306 NFSECPMVYNVLFKRIENSLRMVKIGRDYFYPEKK---------IVLDRRRMEIWPGYVTSIQ---NFDGG--LLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKGGEKISYRDYFKNTYGLQIRDLEQPMIVSK-----SNSRSGKNRGPKGSKEVDGGLVYLIP-ELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCE-EAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDR-YNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNS-----VAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFES-FSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDN----------SKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCD----RLFYL* 975          

BLAST of piwi-1 vs. Smed Unigenes AA
Match: SMU15032988 (SmedSxlregen_c81582_g1_i1|m.21324|m.3914)

HSP 1 Score: 121.324 bits (303), Expect = 5.996e-28
Identity = 114/451 (25.28%), Postives = 197/451 (43.68%), Query Frame = 1

Query: 1138 FMKNWGVQLDNQPITIMGRELNGVDVFSGQQRINAKSPDDWKFGEVKFDIPKGAQHRFAILAV--ERSPSQFNQFIDDVKREISRLRVNYSIETVAT-----CRANEIEDCLTDFVR-----VQKVHLALVFIGDDKAYARVKNF-TISTGLLTQCVTVRNGTNXXXXXXKVVADKSVMQIFAKLGYDPWGIELKMVPT------------MVVGLDTFHSKTGK----RSIQASVFSISSKFSQYLSFVNSPKGKNEY--------HDNLSQNFIAAINTFKDKFNTLPQRFIIYRDGVGDSQLYFTKKYETDAVLKIMETIYENENLPQIIYIIVKKRISVKFF---------KDGNNPNPGTIVDEKVVKPNFYEFYLVSQRTTKGTATPTNYNVLMDTQLTNRKTGEKAPIPAGQLQHITYALTHLYFNWMGTIRVPVPTHYAHRLA 2352
            F++N+ +++D + I +  R L+G +   G QR        W     KF  P      +A+ AV  ER   + ++ + D++ ++     N  I    +          IE   TD +R      +K+ L +  + +D+ Y  VK F  +  G++TQC+  +  T    +         ++++  KLG    G+  ++ P             MV G+D  H  + +    +S+ A V S+ +  + Y + +     K E          +++  N +     +K      P+R I YRDGV + Q     K+E  A+    + + E +  P I YI+V+KR  ++FF         + GN P+ GT+VD+ +     ++FYL S    +GT+ P +Y+VL D    N  + E        LQ  TY L H+Y     ++  P PT+YAH  A
Sbjct:  540 FLQNYKIEIDPKLIGVDARVLDGPEGIFGGQRTEPLRGGKWDIRNKKFIKPYDKLVNWALFAVGTERDYQRNDKPLYDIEDKLPGAANNVGIRMGGSFYFSYVPYQNIEKVATDTIRNSEKEKKKLDLLVFVVINDEDYKEVKKFGDVLFGIVTQCM--KYATLNRQKFLDQYLGNIMLKVNGKLG----GVNWELSPIRNRLFTPIKDSFMVFGIDVTHPASTRNDLSKSVAAMVGSLDNNATMYSALIRQQLSKKERAIIEIVMEMEDMVYNLLMTF--YKANKQLKPKRLIFYRDGVSEGQFDIVFKHEIIAIQSACQKL-EKDYQPPITYIVVQKRHKIRFFPQNEKDGVGRSGNVPS-GTVVDKDITHCREFDFYLCSHEGIQGTSKPAHYHVLFDE--NNWGSDE--------LQSFTYFLCHIYARCTRSVSYPAPTYYAHLAA 970          

BLAST of piwi-1 vs. Smed Unigenes AA
Match: SMU15015027 (SmedAsxldir_c97534_g1_i3|m.40524|m.26292)

HSP 1 Score: 105.531 bits (262), Expect = 3.101e-26
Identity = 49/73 (67.12%), Postives = 57/73 (78.08%), Query Frame = 1

Query:  778 MDVNDEIELNGTKISYANYFKQRYNITLNNGKQPFVISRLRRVAIQQKENQENEPEAPKEADQSLSIPGELCF 996
            MDVNDE++L   KISY NYFKQRYNI L N +QPFVISR++R  ++QKE  ENEPE P E DQSLSI GEL +
Sbjct:    1 MDVNDEVQLGDKKISYLNYFKQRYNINLKNDRQPFVISRVKRSVVRQKEKIENEPEGPTETDQSLSISGELGY 73          

BLAST of piwi-1 vs. RefSeq Human
Match: gi|66346725|ref|NP_004755.2| (piwi-like protein 1 isoform 1 [Homo sapiens])

HSP 1 Score: 257.299 bits (656), Expect = 7.130e-72
Identity = 207/748 (27.67%), Postives = 345/748 (46.12%), Query Frame = 1

Query:  298 FDGRRLFTSSQWHQEDIETISI----KDEEVSIRFLASFVENTEEYYQMVNIIFNNIQAFMGQERIGRAFFLGSNSNESTSKCGPSFFKRDNFKILGGFATTLSRGTNSDGSASTLLYLERINRVLNENSV----YNIYNKNQLDL--------LIGRDVITKYNNKTYRISDIKEMDVNDEI---ELNGTKISYANYFKQRYNITLNNGKQPFVISRLRRVAIQQKENQENEPEAPKEADQSLSIPGELCFLCGFSDSERSNINLQKSLGSVLKREPRERLKDISDFCSWTDEG-KAAPFMKNWGVQLDNQPITIMGRELNGVDVFSGQQRI--NAKSPDDWKFGEVKFDIPKGAQHRFAILAVERSPSQFNQFIDDVKREISRLRVNYSIETVATCRANEIEDCLTDFVRV--QKV----HLALVFIGD---DKAYARVKNFTISTGLLTQCVTVRNGTNXXXXXXKVVADKSVMQIFAKLGYDPWGIELKMVPTMVVGLDTFHSKT-GKRSIQASVFSISSKFSQYLSFVNSPKGKNEYHDNLSQNFIAAINTFKDKFNTLPQRFIIYRDGVGDSQLYFTKKYETDAVLKIMETIYENENLPQIIYIIVKKRISVKFFKDGN----NPNPGTIVDEKVVKPNFYEFYLVSQRTTKGTATPTNYNVLMDTQLTNRKTGEKAPIPAGQLQHITYALTHLYFNWMGTIRVPVPTHYAHRLAELVG-TVHRGTNPPVISDRIRERLFYL 2430
            FDG  LF   +  Q+  E  S     +D  ++I         +    Q  NIIF  +   M  ++IGR ++   N N+      P         I  GF T++ +  NS      +L  +  ++VL   +V    +N Y++ +           LIG  V+TKYNNKTYR+ DI + D N +    + +G+++S+  Y++++YN  + + KQP ++S       Q K  +      P  A   + IP ELC+L G +D  R++ N+ K L    +  P +R +++     +  +       +++WG+  D+  ++  GR L    +  G +    N +  D  K       I       + ++   R+    N  I ++ +    + +      +      E++D    ++RV  QKV     + +  +     DK  A  K         +QCV  R  T    +    +A K  +Q+  K+G + W +++ +   M+VG+D +H  T G+RSI   V SI+   +++ S         E  D L     AA+  +      +P R I+YRDGVGD QL     YE    L  +++I    N P++  I+VKKR++ +FF        NP PGT++D +V +P +Y+F++VSQ    G+ +PT+YNV+ D           + +    +Q +TY L H+Y+NW G IRVP P  YAH+LA LVG ++HR  N       +  RL+YL
Sbjct:  163 FDGTILFLPKRLQQKVTEVFSKTRNGEDVRITITLTNELPPTSPTCLQFYNIIFRRLLKIMNLQQIGRNYY---NPND------PIDIPSHRLVIWPGFTTSILQYENS-----IMLCTDVSHKVLRSETVLDFMFNFYHQTEEHKFQEQVSKELIGLVVLTKYNNKTYRVDDI-DWDQNPKSTFKKADGSEVSFLEYYRKQYNQEITDLKQPVLVS-------QPKRRRGPGGTLPGPA---MLIP-ELCYLTGLTDKMRNDFNVMKDLAVHTRLTPEQRQREVGRLIDYIHKNDNVQRELRDWGLSFDSNLLSFSGRILQTEKIHQGGKTFDYNPQFADWSKETRGAPLISVKPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGMQMRKAIMI-----EVDDRTEAYLRVLQQKVTADTQIVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVAR--TLGKQQTVMAIATKIALQMNCKMGGELWRVDIPLKLVMIVGIDCYHDMTAGRRSIAGFVASINEGMTRWFSRCIFQDRGQELVDGLKVCLQAALRAWNSCNEYMPSRIIVYRDGVGDGQLKTLVNYEVPQFLDCLKSIGRGYN-PRLTVIVVKKRVNTRFFAQSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDN----------SGLKPDHIQRLTYKLCHIYYNWPGVIRVPAPCQYAHKLAFLVGQSIHREPNL-----SLSNRLYYL 861          

BLAST of piwi-1 vs. RefSeq Human
Match: gi|530401794|ref|XP_005253689.1| (PREDICTED: piwi-like protein 1 isoform X2 [Homo sapiens])

HSP 1 Score: 257.299 bits (656), Expect = 7.130e-72
Identity = 207/748 (27.67%), Postives = 345/748 (46.12%), Query Frame = 1

Query:  298 FDGRRLFTSSQWHQEDIETISI----KDEEVSIRFLASFVENTEEYYQMVNIIFNNIQAFMGQERIGRAFFLGSNSNESTSKCGPSFFKRDNFKILGGFATTLSRGTNSDGSASTLLYLERINRVLNENSV----YNIYNKNQLDL--------LIGRDVITKYNNKTYRISDIKEMDVNDEI---ELNGTKISYANYFKQRYNITLNNGKQPFVISRLRRVAIQQKENQENEPEAPKEADQSLSIPGELCFLCGFSDSERSNINLQKSLGSVLKREPRERLKDISDFCSWTDEG-KAAPFMKNWGVQLDNQPITIMGRELNGVDVFSGQQRI--NAKSPDDWKFGEVKFDIPKGAQHRFAILAVERSPSQFNQFIDDVKREISRLRVNYSIETVATCRANEIEDCLTDFVRV--QKV----HLALVFIGD---DKAYARVKNFTISTGLLTQCVTVRNGTNXXXXXXKVVADKSVMQIFAKLGYDPWGIELKMVPTMVVGLDTFHSKT-GKRSIQASVFSISSKFSQYLSFVNSPKGKNEYHDNLSQNFIAAINTFKDKFNTLPQRFIIYRDGVGDSQLYFTKKYETDAVLKIMETIYENENLPQIIYIIVKKRISVKFFKDGN----NPNPGTIVDEKVVKPNFYEFYLVSQRTTKGTATPTNYNVLMDTQLTNRKTGEKAPIPAGQLQHITYALTHLYFNWMGTIRVPVPTHYAHRLAELVG-TVHRGTNPPVISDRIRERLFYL 2430
            FDG  LF   +  Q+  E  S     +D  ++I         +    Q  NIIF  +   M  ++IGR ++   N N+      P         I  GF T++ +  NS      +L  +  ++VL   +V    +N Y++ +           LIG  V+TKYNNKTYR+ DI + D N +    + +G+++S+  Y++++YN  + + KQP ++S       Q K  +      P  A   + IP ELC+L G +D  R++ N+ K L    +  P +R +++     +  +       +++WG+  D+  ++  GR L    +  G +    N +  D  K       I       + ++   R+    N  I ++ +    + +      +      E++D    ++RV  QKV     + +  +     DK  A  K         +QCV  R  T    +    +A K  +Q+  K+G + W +++ +   M+VG+D +H  T G+RSI   V SI+   +++ S         E  D L     AA+  +      +P R I+YRDGVGD QL     YE    L  +++I    N P++  I+VKKR++ +FF        NP PGT++D +V +P +Y+F++VSQ    G+ +PT+YNV+ D           + +    +Q +TY L H+Y+NW G IRVP P  YAH+LA LVG ++HR  N       +  RL+YL
Sbjct:  163 FDGTILFLPKRLQQKVTEVFSKTRNGEDVRITITLTNELPPTSPTCLQFYNIIFRRLLKIMNLQQIGRNYY---NPND------PIDIPSHRLVIWPGFTTSILQYENS-----IMLCTDVSHKVLRSETVLDFMFNFYHQTEEHKFQEQVSKELIGLVVLTKYNNKTYRVDDI-DWDQNPKSTFKKADGSEVSFLEYYRKQYNQEITDLKQPVLVS-------QPKRRRGPGGTLPGPA---MLIP-ELCYLTGLTDKMRNDFNVMKDLAVHTRLTPEQRQREVGRLIDYIHKNDNVQRELRDWGLSFDSNLLSFSGRILQTEKIHQGGKTFDYNPQFADWSKETRGAPLISVKPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGMQMRKAIMI-----EVDDRTEAYLRVLQQKVTADTQIVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVAR--TLGKQQTVMAIATKIALQMNCKMGGELWRVDIPLKLVMIVGIDCYHDMTAGRRSIAGFVASINEGMTRWFSRCIFQDRGQELVDGLKVCLQAALRAWNSCNEYMPSRIIVYRDGVGDGQLKTLVNYEVPQFLDCLKSIGRGYN-PRLTVIVVKKRVNTRFFAQSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDN----------SGLKPDHIQRLTYKLCHIYYNWPGVIRVPAPCQYAHKLAFLVGQSIHREPNL-----SLSNRLYYL 861          

BLAST of piwi-1 vs. RefSeq Human
Match: gi|578824471|ref|XP_006719761.1| (PREDICTED: piwi-like protein 1 isoform X2 [Homo sapiens])

HSP 1 Score: 257.299 bits (656), Expect = 7.130e-72
Identity = 207/748 (27.67%), Postives = 345/748 (46.12%), Query Frame = 1

Query:  298 FDGRRLFTSSQWHQEDIETISI----KDEEVSIRFLASFVENTEEYYQMVNIIFNNIQAFMGQERIGRAFFLGSNSNESTSKCGPSFFKRDNFKILGGFATTLSRGTNSDGSASTLLYLERINRVLNENSV----YNIYNKNQLDL--------LIGRDVITKYNNKTYRISDIKEMDVNDEI---ELNGTKISYANYFKQRYNITLNNGKQPFVISRLRRVAIQQKENQENEPEAPKEADQSLSIPGELCFLCGFSDSERSNINLQKSLGSVLKREPRERLKDISDFCSWTDEG-KAAPFMKNWGVQLDNQPITIMGRELNGVDVFSGQQRI--NAKSPDDWKFGEVKFDIPKGAQHRFAILAVERSPSQFNQFIDDVKREISRLRVNYSIETVATCRANEIEDCLTDFVRV--QKV----HLALVFIGD---DKAYARVKNFTISTGLLTQCVTVRNGTNXXXXXXKVVADKSVMQIFAKLGYDPWGIELKMVPTMVVGLDTFHSKT-GKRSIQASVFSISSKFSQYLSFVNSPKGKNEYHDNLSQNFIAAINTFKDKFNTLPQRFIIYRDGVGDSQLYFTKKYETDAVLKIMETIYENENLPQIIYIIVKKRISVKFFKDGN----NPNPGTIVDEKVVKPNFYEFYLVSQRTTKGTATPTNYNVLMDTQLTNRKTGEKAPIPAGQLQHITYALTHLYFNWMGTIRVPVPTHYAHRLAELVG-TVHRGTNPPVISDRIRERLFYL 2430
            FDG  LF   +  Q+  E  S     +D  ++I         +    Q  NIIF  +   M  ++IGR ++   N N+      P         I  GF T++ +  NS      +L  +  ++VL   +V    +N Y++ +           LIG  V+TKYNNKTYR+ DI + D N +    + +G+++S+  Y++++YN  + + KQP ++S       Q K  +      P  A   + IP ELC+L G +D  R++ N+ K L    +  P +R +++     +  +       +++WG+  D+  ++  GR L    +  G +    N +  D  K       I       + ++   R+    N  I ++ +    + +      +      E++D    ++RV  QKV     + +  +     DK  A  K         +QCV  R  T    +    +A K  +Q+  K+G + W +++ +   M+VG+D +H  T G+RSI   V SI+   +++ S         E  D L     AA+  +      +P R I+YRDGVGD QL     YE    L  +++I    N P++  I+VKKR++ +FF        NP PGT++D +V +P +Y+F++VSQ    G+ +PT+YNV+ D           + +    +Q +TY L H+Y+NW G IRVP P  YAH+LA LVG ++HR  N       +  RL+YL
Sbjct:  163 FDGTILFLPKRLQQKVTEVFSKTRNGEDVRITITLTNELPPTSPTCLQFYNIIFRRLLKIMNLQQIGRNYY---NPND------PIDIPSHRLVIWPGFTTSILQYENS-----IMLCTDVSHKVLRSETVLDFMFNFYHQTEEHKFQEQVSKELIGLVVLTKYNNKTYRVDDI-DWDQNPKSTFKKADGSEVSFLEYYRKQYNQEITDLKQPVLVS-------QPKRRRGPGGTLPGPA---MLIP-ELCYLTGLTDKMRNDFNVMKDLAVHTRLTPEQRQREVGRLIDYIHKNDNVQRELRDWGLSFDSNLLSFSGRILQTEKIHQGGKTFDYNPQFADWSKETRGAPLISVKPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGMQMRKAIMI-----EVDDRTEAYLRVLQQKVTADTQIVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVAR--TLGKQQTVMAIATKIALQMNCKMGGELWRVDIPLKLVMIVGIDCYHDMTAGRRSIAGFVASINEGMTRWFSRCIFQDRGQELVDGLKVCLQAALRAWNSCNEYMPSRIIVYRDGVGDGQLKTLVNYEVPQFLDCLKSIGRGYN-PRLTVIVVKKRVNTRFFAQSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDN----------SGLKPDHIQRLTYKLCHIYYNWPGVIRVPAPCQYAHKLAFLVGQSIHREPNL-----SLSNRLYYL 861          

BLAST of piwi-1 vs. RefSeq Human
Match: gi|767975969|ref|XP_011537304.1| (PREDICTED: piwi-like protein 1 isoform X2 [Homo sapiens])

HSP 1 Score: 257.299 bits (656), Expect = 7.130e-72
Identity = 207/748 (27.67%), Postives = 345/748 (46.12%), Query Frame = 1

Query:  298 FDGRRLFTSSQWHQEDIETISI----KDEEVSIRFLASFVENTEEYYQMVNIIFNNIQAFMGQERIGRAFFLGSNSNESTSKCGPSFFKRDNFKILGGFATTLSRGTNSDGSASTLLYLERINRVLNENSV----YNIYNKNQLDL--------LIGRDVITKYNNKTYRISDIKEMDVNDEI---ELNGTKISYANYFKQRYNITLNNGKQPFVISRLRRVAIQQKENQENEPEAPKEADQSLSIPGELCFLCGFSDSERSNINLQKSLGSVLKREPRERLKDISDFCSWTDEG-KAAPFMKNWGVQLDNQPITIMGRELNGVDVFSGQQRI--NAKSPDDWKFGEVKFDIPKGAQHRFAILAVERSPSQFNQFIDDVKREISRLRVNYSIETVATCRANEIEDCLTDFVRV--QKV----HLALVFIGD---DKAYARVKNFTISTGLLTQCVTVRNGTNXXXXXXKVVADKSVMQIFAKLGYDPWGIELKMVPTMVVGLDTFHSKT-GKRSIQASVFSISSKFSQYLSFVNSPKGKNEYHDNLSQNFIAAINTFKDKFNTLPQRFIIYRDGVGDSQLYFTKKYETDAVLKIMETIYENENLPQIIYIIVKKRISVKFFKDGN----NPNPGTIVDEKVVKPNFYEFYLVSQRTTKGTATPTNYNVLMDTQLTNRKTGEKAPIPAGQLQHITYALTHLYFNWMGTIRVPVPTHYAHRLAELVG-TVHRGTNPPVISDRIRERLFYL 2430
            FDG  LF   +  Q+  E  S     +D  ++I         +    Q  NIIF  +   M  ++IGR ++   N N+      P         I  GF T++ +  NS      +L  +  ++VL   +V    +N Y++ +           LIG  V+TKYNNKTYR+ DI + D N +    + +G+++S+  Y++++YN  + + KQP ++S       Q K  +      P  A   + IP ELC+L G +D  R++ N+ K L    +  P +R +++     +  +       +++WG+  D+  ++  GR L    +  G +    N +  D  K       I       + ++   R+    N  I ++ +    + +      +      E++D    ++RV  QKV     + +  +     DK  A  K         +QCV  R  T    +    +A K  +Q+  K+G + W +++ +   M+VG+D +H  T G+RSI   V SI+   +++ S         E  D L     AA+  +      +P R I+YRDGVGD QL     YE    L  +++I    N P++  I+VKKR++ +FF        NP PGT++D +V +P +Y+F++VSQ    G+ +PT+YNV+ D           + +    +Q +TY L H+Y+NW G IRVP P  YAH+LA LVG ++HR  N       +  RL+YL
Sbjct:  163 FDGTILFLPKRLQQKVTEVFSKTRNGEDVRITITLTNELPPTSPTCLQFYNIIFRRLLKIMNLQQIGRNYY---NPND------PIDIPSHRLVIWPGFTTSILQYENS-----IMLCTDVSHKVLRSETVLDFMFNFYHQTEEHKFQEQVSKELIGLVVLTKYNNKTYRVDDI-DWDQNPKSTFKKADGSEVSFLEYYRKQYNQEITDLKQPVLVS-------QPKRRRGPGGTLPGPA---MLIP-ELCYLTGLTDKMRNDFNVMKDLAVHTRLTPEQRQREVGRLIDYIHKNDNVQRELRDWGLSFDSNLLSFSGRILQTEKIHQGGKTFDYNPQFADWSKETRGAPLISVKPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGMQMRKAIMI-----EVDDRTEAYLRVLQQKVTADTQIVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVAR--TLGKQQTVMAIATKIALQMNCKMGGELWRVDIPLKLVMIVGIDCYHDMTAGRRSIAGFVASINEGMTRWFSRCIFQDRGQELVDGLKVCLQAALRAWNSCNEYMPSRIIVYRDGVGDGQLKTLVNYEVPQFLDCLKSIGRGYN-PRLTVIVVKKRVNTRFFAQSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDN----------SGLKPDHIQRLTYKLCHIYYNWPGVIRVPAPCQYAHKLAFLVGQSIHREPNL-----SLSNRLYYL 861          

BLAST of piwi-1 vs. RefSeq Human
Match: gi|767975971|ref|XP_011537305.1| (PREDICTED: piwi-like protein 1 isoform X2 [Homo sapiens])

HSP 1 Score: 257.299 bits (656), Expect = 7.130e-72
Identity = 207/748 (27.67%), Postives = 345/748 (46.12%), Query Frame = 1

Query:  298 FDGRRLFTSSQWHQEDIETISI----KDEEVSIRFLASFVENTEEYYQMVNIIFNNIQAFMGQERIGRAFFLGSNSNESTSKCGPSFFKRDNFKILGGFATTLSRGTNSDGSASTLLYLERINRVLNENSV----YNIYNKNQLDL--------LIGRDVITKYNNKTYRISDIKEMDVNDEI---ELNGTKISYANYFKQRYNITLNNGKQPFVISRLRRVAIQQKENQENEPEAPKEADQSLSIPGELCFLCGFSDSERSNINLQKSLGSVLKREPRERLKDISDFCSWTDEG-KAAPFMKNWGVQLDNQPITIMGRELNGVDVFSGQQRI--NAKSPDDWKFGEVKFDIPKGAQHRFAILAVERSPSQFNQFIDDVKREISRLRVNYSIETVATCRANEIEDCLTDFVRV--QKV----HLALVFIGD---DKAYARVKNFTISTGLLTQCVTVRNGTNXXXXXXKVVADKSVMQIFAKLGYDPWGIELKMVPTMVVGLDTFHSKT-GKRSIQASVFSISSKFSQYLSFVNSPKGKNEYHDNLSQNFIAAINTFKDKFNTLPQRFIIYRDGVGDSQLYFTKKYETDAVLKIMETIYENENLPQIIYIIVKKRISVKFFKDGN----NPNPGTIVDEKVVKPNFYEFYLVSQRTTKGTATPTNYNVLMDTQLTNRKTGEKAPIPAGQLQHITYALTHLYFNWMGTIRVPVPTHYAHRLAELVG-TVHRGTNPPVISDRIRERLFYL 2430
            FDG  LF   +  Q+  E  S     +D  ++I         +    Q  NIIF  +   M  ++IGR ++   N N+      P         I  GF T++ +  NS      +L  +  ++VL   +V    +N Y++ +           LIG  V+TKYNNKTYR+ DI + D N +    + +G+++S+  Y++++YN  + + KQP ++S       Q K  +      P  A   + IP ELC+L G +D  R++ N+ K L    +  P +R +++     +  +       +++WG+  D+  ++  GR L    +  G +    N +  D  K       I       + ++   R+    N  I ++ +    + +      +      E++D    ++RV  QKV     + +  +     DK  A  K         +QCV  R  T    +    +A K  +Q+  K+G + W +++ +   M+VG+D +H  T G+RSI   V SI+   +++ S         E  D L     AA+  +      +P R I+YRDGVGD QL     YE    L  +++I    N P++  I+VKKR++ +FF        NP PGT++D +V +P +Y+F++VSQ    G+ +PT+YNV+ D           + +    +Q +TY L H+Y+NW G IRVP P  YAH+LA LVG ++HR  N       +  RL+YL
Sbjct:  163 FDGTILFLPKRLQQKVTEVFSKTRNGEDVRITITLTNELPPTSPTCLQFYNIIFRRLLKIMNLQQIGRNYY---NPND------PIDIPSHRLVIWPGFTTSILQYENS-----IMLCTDVSHKVLRSETVLDFMFNFYHQTEEHKFQEQVSKELIGLVVLTKYNNKTYRVDDI-DWDQNPKSTFKKADGSEVSFLEYYRKQYNQEITDLKQPVLVS-------QPKRRRGPGGTLPGPA---MLIP-ELCYLTGLTDKMRNDFNVMKDLAVHTRLTPEQRQREVGRLIDYIHKNDNVQRELRDWGLSFDSNLLSFSGRILQTEKIHQGGKTFDYNPQFADWSKETRGAPLISVKPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGMQMRKAIMI-----EVDDRTEAYLRVLQQKVTADTQIVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVAR--TLGKQQTVMAIATKIALQMNCKMGGELWRVDIPLKLVMIVGIDCYHDMTAGRRSIAGFVASINEGMTRWFSRCIFQDRGQELVDGLKVCLQAALRAWNSCNEYMPSRIIVYRDGVGDGQLKTLVNYEVPQFLDCLKSIGRGYN-PRLTVIVVKKRVNTRFFAQSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDN----------SGLKPDHIQRLTYKLCHIYYNWPGVIRVPAPCQYAHKLAFLVGQSIHREPNL-----SLSNRLYYL 861          

BLAST of piwi-1 vs. RefSeq Drosophila melanogaster
Match: gi|17136736|ref|NP_476875.1| (piwi, isoform A [Drosophila melanogaster])

HSP 1 Score: 261.151 bits (666), Expect = 9.685e-74
Identity = 201/732 (27.46%), Postives = 343/732 (46.86%), Query Frame = 1

Query:  292 FCFDGRRLFTSSQWHQEDIETISIKDE-----EVSIRFLASFVENTEEYYQMVNIIFNNIQAFMGQERIGRAFFLGSNSNESTSKCGPSFFKRDNFKILGGFATTLSRGTNSDGSASTLLYLERINRVLNENSVYNIYNK--------------NQLDLLIGRDVITKYNNKTYRISDIK-EMDVNDEIELNGTKISYANYFKQRYNITLNNGKQPFVISRLRRVAIQQKENQENEPEAPKEADQSLSIPGELCFLCGFSDSERSNINLQKSLGSVLKREPRERLKDISDF-CSWTDEGKAAPFMKNWGVQLDNQPITIMGRELNGVDVFSGQQRINAKSPDDWK--FGEVK-FDIPKGAQHRFAILAVERSPSQFNQFIDDVKREIS----RLR-----VNYSIETVATCRANEIEDCLTDFVRVQKVHLALVFIGDDKA--YARVKNFT-ISTGLLTQCVTVRNGTNXXXXXXKVVADKSVMQIFAKLGYDPWGIELKMVPTMVVGLDTFHS-KTGKRSIQASVFSIS-SKFSQYLSFVNSPKGKNEYHDNLSQNFIAAINTFKDKFNTLPQRFIIYRDGVGDSQLYFTKKYETDAVLKIMETIYENENL--PQIIYIIVKKRISVKFFKDGNNPNPGTIVDEKVVKPNFYEFYLVSQRTTKGTATPTNYNVLMDTQLTNRKTGEKAPIPAGQLQHITYALTHLYFNWMGTIRVPVPTHYAHRLAELVGT 2367
            + FDG +LFT+ ++ QE I  +S K +     ++SI+F+         + Q++N+I       +  E +GR  F      +  +K     FK    ++  G+ T++ +          LL  E  ++V+   ++Y+I  +              N LDL+    V+T YNN+TYRI+D+             G  IS+  Y+  +YNI + +  QP +IS+ R  A++              A + + +  ELC + G +   RSN  L +++ S  +  P++R   +  F     +  ++   +++W ++LD     + GR +   ++     ++ A    DW+  F + +    P     R+A++A +R+  +    +D + R  S    R+R     + Y   T    RA  ++DC+          L L  + +D A  Y+ +K    +   + TQ VT++   NR       +A K  +Q+  KLGY PW IEL +   M +G D   S +  KR+  A + S+   + S Y S V      +   + L      A+  ++ +   LP R + YRDGV    L    ++E   +++ ++T Y    L  PQ+ YI+V + ++ +FF +G NP PGTIVD+ +  P  Y+FYLVSQ+  +GT +PT+YNVL  +            +   ++Q +TY + HLY+NW GT RVP    YA +LA LVGT
Sbjct:  146 YLFDGLQLFTTRKFEQE-ITVLSGKSKLDIEYKISIKFVGFISCAEPRFLQVLNLILRRSMKGLNLELVGRNLF------DPRAKIEIREFK---MELWPGYETSIRQH-----EKDILLGTEITHKVMRTETIYDIMRRCSHNPARHQDEVRVNVLDLI----VLTDYNNRTYRINDVDFGQTPKSTFSCKGRDISFVEYYLTKYNIRIRDHNQPLLISKNRDKALK------------TNASELVVLIPELCRVTGLNAEMRSNFQLMRAMSSYTRMNPKQRTDRLRAFNHRLQNTPESVKVLRDWNMELDKNVTEVQGRIIGQQNIVFHNGKVPAGENADWQRHFRDQRMLTTPSDGLDRWAVIAPQRNSHELRTLLDSLYRAASGMGLRIRSPQEFIIYDDRTGTYVRA--MDDCVRS-----DPKLILCLVPNDNAERYSSIKKRGYVDRAVPTQVVTLKTTKNRSLMS---IATKIAIQLNCKLGYTPWMIELPLSGLMTIGFDIAKSTRDRKRAYGALIASMDLQQNSTYFSTVTECSAFDVLANTLWPMIAKALRQYQHEHRKLPSRIVFYRDGVSSGSLKQLFEFEVKDIIEKLKTEYARVQLSPPQLAYIVVTRSMNTRFFLNGQNPPPGTIVDDVITLPERYDFYLVSQQVRQGTVSPTSYNVLYSSM----------GLSPEKMQKLTYKMCHLYYNWSGTTRVPAVCQYAKKLATLVGT 826          

BLAST of piwi-1 vs. RefSeq Drosophila melanogaster
Match: gi|665407544|ref|NP_001285825.1| (piwi, isoform B [Drosophila melanogaster])

HSP 1 Score: 261.151 bits (666), Expect = 9.685e-74
Identity = 201/732 (27.46%), Postives = 343/732 (46.86%), Query Frame = 1

Query:  292 FCFDGRRLFTSSQWHQEDIETISIKDE-----EVSIRFLASFVENTEEYYQMVNIIFNNIQAFMGQERIGRAFFLGSNSNESTSKCGPSFFKRDNFKILGGFATTLSRGTNSDGSASTLLYLERINRVLNENSVYNIYNK--------------NQLDLLIGRDVITKYNNKTYRISDIK-EMDVNDEIELNGTKISYANYFKQRYNITLNNGKQPFVISRLRRVAIQQKENQENEPEAPKEADQSLSIPGELCFLCGFSDSERSNINLQKSLGSVLKREPRERLKDISDF-CSWTDEGKAAPFMKNWGVQLDNQPITIMGRELNGVDVFSGQQRINAKSPDDWK--FGEVK-FDIPKGAQHRFAILAVERSPSQFNQFIDDVKREIS----RLR-----VNYSIETVATCRANEIEDCLTDFVRVQKVHLALVFIGDDKA--YARVKNFT-ISTGLLTQCVTVRNGTNXXXXXXKVVADKSVMQIFAKLGYDPWGIELKMVPTMVVGLDTFHS-KTGKRSIQASVFSIS-SKFSQYLSFVNSPKGKNEYHDNLSQNFIAAINTFKDKFNTLPQRFIIYRDGVGDSQLYFTKKYETDAVLKIMETIYENENL--PQIIYIIVKKRISVKFFKDGNNPNPGTIVDEKVVKPNFYEFYLVSQRTTKGTATPTNYNVLMDTQLTNRKTGEKAPIPAGQLQHITYALTHLYFNWMGTIRVPVPTHYAHRLAELVGT 2367
            + FDG +LFT+ ++ QE I  +S K +     ++SI+F+         + Q++N+I       +  E +GR  F      +  +K     FK    ++  G+ T++ +          LL  E  ++V+   ++Y+I  +              N LDL+    V+T YNN+TYRI+D+             G  IS+  Y+  +YNI + +  QP +IS+ R  A++              A + + +  ELC + G +   RSN  L +++ S  +  P++R   +  F     +  ++   +++W ++LD     + GR +   ++     ++ A    DW+  F + +    P     R+A++A +R+  +    +D + R  S    R+R     + Y   T    RA  ++DC+          L L  + +D A  Y+ +K    +   + TQ VT++   NR       +A K  +Q+  KLGY PW IEL +   M +G D   S +  KR+  A + S+   + S Y S V      +   + L      A+  ++ +   LP R + YRDGV    L    ++E   +++ ++T Y    L  PQ+ YI+V + ++ +FF +G NP PGTIVD+ +  P  Y+FYLVSQ+  +GT +PT+YNVL  +            +   ++Q +TY + HLY+NW GT RVP    YA +LA LVGT
Sbjct:  146 YLFDGLQLFTTRKFEQE-ITVLSGKSKLDIEYKISIKFVGFISCAEPRFLQVLNLILRRSMKGLNLELVGRNLF------DPRAKIEIREFK---MELWPGYETSIRQH-----EKDILLGTEITHKVMRTETIYDIMRRCSHNPARHQDEVRVNVLDLI----VLTDYNNRTYRINDVDFGQTPKSTFSCKGRDISFVEYYLTKYNIRIRDHNQPLLISKNRDKALK------------TNASELVVLIPELCRVTGLNAEMRSNFQLMRAMSSYTRMNPKQRTDRLRAFNHRLQNTPESVKVLRDWNMELDKNVTEVQGRIIGQQNIVFHNGKVPAGENADWQRHFRDQRMLTTPSDGLDRWAVIAPQRNSHELRTLLDSLYRAASGMGLRIRSPQEFIIYDDRTGTYVRA--MDDCVRS-----DPKLILCLVPNDNAERYSSIKKRGYVDRAVPTQVVTLKTTKNRSLMS---IATKIAIQLNCKLGYTPWMIELPLSGLMTIGFDIAKSTRDRKRAYGALIASMDLQQNSTYFSTVTECSAFDVLANTLWPMIAKALRQYQHEHRKLPSRIVFYRDGVSSGSLKQLFEFEVKDIIEKLKTEYARVQLSPPQLAYIVVTRSMNTRFFLNGQNPPPGTIVDDVITLPERYDFYLVSQQVRQGTVSPTSYNVLYSSM----------GLSPEKMQKLTYKMCHLYYNWSGTTRVPAVCQYAKKLATLVGT 826          

BLAST of piwi-1 vs. RefSeq Drosophila melanogaster
Match: gi|221513719|ref|NP_001036627.2| (argonaute 3, isoform D [Drosophila melanogaster])

HSP 1 Score: 246.514 bits (628), Expect = 1.174e-68
Identity = 205/775 (26.45%), Postives = 352/775 (45.42%), Query Frame = 1

Query:  202 FMYDVKM--PPKSLRGKLDLLNS----VASTKENRPFCFDGRRLFTSSQWHQEDIETIS-IKDEEVSIRFLASFVENTEEYYQMVNIIFNNIQAFMGQERIGRAFFLGSNSNESTSKCGPSFFKRDNFKILGGFATTLSRGTNSDGSASTLLYLERINRVLNENSVYNI-----------YNKNQLDLLIGRDVITKYNNKTYRISDIK-EMDVNDEIELNGTKISYANYFKQRYNITLNNGKQPFVISRLRRVAIQQKENQENEPEAPKEADQSLSIPGELCFLCGFSDSERSNINLQKSLGSVLKREPRERLKDISDFCSWTDEGKAA-PFMKNWGVQLDNQPITIMGRELNGVDVFSGQQRINAKSPDDWKFGEVKFDIPKGAQ-HRFAILAVERSPSQFNQFIDDVKREISRLRVNYSIETVATCRANEIEDCLTDFVRVQKVHLALVFI----GDDKAYARVKNFTIS-TGLLTQCVTVRNGTNXXXXXXKVVADKSVMQIFAKLGYDPWGIELKMVPTMVVGLDTFHSKTGK-RSIQASVFSISSKFSQYLSFVNSPKGKNEYHDNLSQNFIAAINTFKDKFNTLPQRFIIYRDGVGDSQLYFTKKYETDAVLKIMETIYENENLPQIIYIIVKKRISVKFFKDGN----NPNPGTIVDEKVVKPNFYEFYLVSQRTTKGTATPTNYNVLMDTQLTNRKTGEKAPIPAGQLQHITYALTHLYFNWMGTIRVPVPTHYAHRLAELVG-TVHRGTNPPVISDRIRERLFYL 2430
            F Y+V+   P  S+  ++  LN     +  TK      FDG  L+       +    IS  +D E+ IR L    E      Q+ NI+F+ +   +   +  R  F  S          P        ++  G+ T +      +     +L  +  +R+L + +V  +           Y ++   +L+G  V+T+YNN+TY+I+DI  + +   + E+     SY  Y+KQ +NI + +  QP + S  +   I           A +E  Q   IP ELC+L G  D  RS+  L + + +  +  P +R   ++ F        AA   + +WG+ L N    I GR+++   ++  +  ++A    ++    V  ++ K     ++ I+ +          +D++K+    L +N S  T+ +   + I+  +    R   ++  +V        D  YA +K    S   + +Q +  +   N      + V  K V+Q+  KLG   W +++     M+ G+D++H  + +  S+ A V SI+S +SQ+ S       + E  + LS +F  A+  ++ +   LP   IIYRDG+GD QLY    YE    +   E +  N    +I YI+V+KRI+ + F        NP PGT+VD+ + K N Y+F+LVSQ   +GT TPT+Y VL D                  +Q ++Y L  LY+NW GT+R+P    YAH+LA L+G ++ R      +++ + E+LFYL
Sbjct:  146 FNYEVRFFPPIDSVHLRIKYLNDHKDKLGGTKT-----FDGNTLYLPILLPNKMTVFISKAEDVELQIRILYKKKEEMRNCTQLYNILFDRVMKVLNYVKFDRKQFDPSR---------PKIIPLAKLEVWPGYVTAVD-----EYKGGLMLCCDVSHRILCQKTVLEMLVDLYQQNVEHYQESARKMLVGNIVLTRYNNRTYKINDICFDQNPTCQFEIKTGCTSYVEYYKQYHNINIKDVNQPLIYSIKKSRGI----------PAERENLQFCLIP-ELCYLTGLRDEVRSDNKLMREIATFTRVSPNQRQMALNKFYENVSNTPAAQEILNSWGLSLTNNSNKISGRQMDIEQIYFSKISVSAGRSAEFSKHAVTNEMLKVVHLSKWIIIHLRNYRQAATSLLDNMKQACESLGMNISNPTMISLDHDRIDAYIQALRRNITMNTQMVVCICHNRRDDRYAAIKKICCSEIPIPSQVINAKTLQNDLKI--RSVVQKIVLQMNCKLGGSLWTVKIPFKNVMICGIDSYHDPSNRGNSVAAFVASINSSYSQWYSKAVVQTKREEIVNGLSASFEIALKMYRKRNGKLPTNIIIYRDGIGDGQLYTCLNYE----IPQFEMVCGNR--IKISYIVVQKRINTRIFSGSGIHLENPLPGTVVDQHITKSNMYDFFLVSQLVRQGTVTPTHYVVLRD----------DCNYGPDIIQKLSYKLCFLYYNWAGTVRIPACCMYAHKLAYLIGQSIQRD-----VAEALSEKLFYL 867          

BLAST of piwi-1 vs. RefSeq Drosophila melanogaster
Match: gi|221513722|ref|NP_001036629.2| (argonaute 3, isoform E [Drosophila melanogaster])

HSP 1 Score: 246.514 bits (628), Expect = 1.174e-68
Identity = 205/775 (26.45%), Postives = 352/775 (45.42%), Query Frame = 1

Query:  202 FMYDVKM--PPKSLRGKLDLLNS----VASTKENRPFCFDGRRLFTSSQWHQEDIETIS-IKDEEVSIRFLASFVENTEEYYQMVNIIFNNIQAFMGQERIGRAFFLGSNSNESTSKCGPSFFKRDNFKILGGFATTLSRGTNSDGSASTLLYLERINRVLNENSVYNI-----------YNKNQLDLLIGRDVITKYNNKTYRISDIK-EMDVNDEIELNGTKISYANYFKQRYNITLNNGKQPFVISRLRRVAIQQKENQENEPEAPKEADQSLSIPGELCFLCGFSDSERSNINLQKSLGSVLKREPRERLKDISDFCSWTDEGKAA-PFMKNWGVQLDNQPITIMGRELNGVDVFSGQQRINAKSPDDWKFGEVKFDIPKGAQ-HRFAILAVERSPSQFNQFIDDVKREISRLRVNYSIETVATCRANEIEDCLTDFVRVQKVHLALVFI----GDDKAYARVKNFTIS-TGLLTQCVTVRNGTNXXXXXXKVVADKSVMQIFAKLGYDPWGIELKMVPTMVVGLDTFHSKTGK-RSIQASVFSISSKFSQYLSFVNSPKGKNEYHDNLSQNFIAAINTFKDKFNTLPQRFIIYRDGVGDSQLYFTKKYETDAVLKIMETIYENENLPQIIYIIVKKRISVKFFKDGN----NPNPGTIVDEKVVKPNFYEFYLVSQRTTKGTATPTNYNVLMDTQLTNRKTGEKAPIPAGQLQHITYALTHLYFNWMGTIRVPVPTHYAHRLAELVG-TVHRGTNPPVISDRIRERLFYL 2430
            F Y+V+   P  S+  ++  LN     +  TK      FDG  L+       +    IS  +D E+ IR L    E      Q+ NI+F+ +   +   +  R  F  S          P        ++  G+ T +      +     +L  +  +R+L + +V  +           Y ++   +L+G  V+T+YNN+TY+I+DI  + +   + E+     SY  Y+KQ +NI + +  QP + S  +   I           A +E  Q   IP ELC+L G  D  RS+  L + + +  +  P +R   ++ F        AA   + +WG+ L N    I GR+++   ++  +  ++A    ++    V  ++ K     ++ I+ +          +D++K+    L +N S  T+ +   + I+  +    R   ++  +V        D  YA +K    S   + +Q +  +   N      + V  K V+Q+  KLG   W +++     M+ G+D++H  + +  S+ A V SI+S +SQ+ S       + E  + LS +F  A+  ++ +   LP   IIYRDG+GD QLY    YE    +   E +  N    +I YI+V+KRI+ + F        NP PGT+VD+ + K N Y+F+LVSQ   +GT TPT+Y VL D                  +Q ++Y L  LY+NW GT+R+P    YAH+LA L+G ++ R      +++ + E+LFYL
Sbjct:  146 FNYEVRFFPPIDSVHLRIKYLNDHKDKLGGTKT-----FDGNTLYLPILLPNKMTVFISKAEDVELQIRILYKKKEEMRNCTQLYNILFDRVMKVLNYVKFDRKQFDPSR---------PKIIPLAKLEVWPGYVTAVD-----EYKGGLMLCCDVSHRILCQKTVLEMLVDLYQQNVEHYQESARKMLVGNIVLTRYNNRTYKINDICFDQNPTCQFEIKTGCTSYVEYYKQYHNINIKDVNQPLIYSIKKSRGI----------PAERENLQFCLIP-ELCYLTGLRDEVRSDNKLMREIATFTRVSPNQRQMALNKFYENVSNTPAAQEILNSWGLSLTNNSNKISGRQMDIEQIYFSKISVSAGRSAEFSKHAVTNEMLKVVHLSKWIIIHLRNYRQAATSLLDNMKQACESLGMNISNPTMISLDHDRIDAYIQALRRNITMNTQMVVCICHNRRDDRYAAIKKICCSEIPIPSQVINAKTLQNDLKI--RSVVQKIVLQMNCKLGGSLWTVKIPFKNVMICGIDSYHDPSNRGNSVAAFVASINSSYSQWYSKAVVQTKREEIVNGLSASFEIALKMYRKRNGKLPTNIIIYRDGIGDGQLYTCLNYE----IPQFEMVCGNR--IKISYIVVQKRINTRIFSGSGIHLENPLPGTVVDQHITKSNMYDFFLVSQLVRQGTVTPTHYVVLRD----------DCNYGPDIIQKLSYKLCFLYYNWAGTVRIPACCMYAHKLAYLIGQSIQRD-----VAEALSEKLFYL 867          

BLAST of piwi-1 vs. RefSeq Drosophila melanogaster
Match: gi|221513725|ref|NP_001036628.2| (argonaute 3, isoform F [Drosophila melanogaster])

HSP 1 Score: 246.514 bits (628), Expect = 1.174e-68
Identity = 205/775 (26.45%), Postives = 352/775 (45.42%), Query Frame = 1

Query:  202 FMYDVKM--PPKSLRGKLDLLNS----VASTKENRPFCFDGRRLFTSSQWHQEDIETIS-IKDEEVSIRFLASFVENTEEYYQMVNIIFNNIQAFMGQERIGRAFFLGSNSNESTSKCGPSFFKRDNFKILGGFATTLSRGTNSDGSASTLLYLERINRVLNENSVYNI-----------YNKNQLDLLIGRDVITKYNNKTYRISDIK-EMDVNDEIELNGTKISYANYFKQRYNITLNNGKQPFVISRLRRVAIQQKENQENEPEAPKEADQSLSIPGELCFLCGFSDSERSNINLQKSLGSVLKREPRERLKDISDFCSWTDEGKAA-PFMKNWGVQLDNQPITIMGRELNGVDVFSGQQRINAKSPDDWKFGEVKFDIPKGAQ-HRFAILAVERSPSQFNQFIDDVKREISRLRVNYSIETVATCRANEIEDCLTDFVRVQKVHLALVFI----GDDKAYARVKNFTIS-TGLLTQCVTVRNGTNXXXXXXKVVADKSVMQIFAKLGYDPWGIELKMVPTMVVGLDTFHSKTGK-RSIQASVFSISSKFSQYLSFVNSPKGKNEYHDNLSQNFIAAINTFKDKFNTLPQRFIIYRDGVGDSQLYFTKKYETDAVLKIMETIYENENLPQIIYIIVKKRISVKFFKDGN----NPNPGTIVDEKVVKPNFYEFYLVSQRTTKGTATPTNYNVLMDTQLTNRKTGEKAPIPAGQLQHITYALTHLYFNWMGTIRVPVPTHYAHRLAELVG-TVHRGTNPPVISDRIRERLFYL 2430
            F Y+V+   P  S+  ++  LN     +  TK      FDG  L+       +    IS  +D E+ IR L    E      Q+ NI+F+ +   +   +  R  F  S          P        ++  G+ T +      +     +L  +  +R+L + +V  +           Y ++   +L+G  V+T+YNN+TY+I+DI  + +   + E+     SY  Y+KQ +NI + +  QP + S  +   I           A +E  Q   IP ELC+L G  D  RS+  L + + +  +  P +R   ++ F        AA   + +WG+ L N    I GR+++   ++  +  ++A    ++    V  ++ K     ++ I+ +          +D++K+    L +N S  T+ +   + I+  +    R   ++  +V        D  YA +K    S   + +Q +  +   N      + V  K V+Q+  KLG   W +++     M+ G+D++H  + +  S+ A V SI+S +SQ+ S       + E  + LS +F  A+  ++ +   LP   IIYRDG+GD QLY    YE    +   E +  N    +I YI+V+KRI+ + F        NP PGT+VD+ + K N Y+F+LVSQ   +GT TPT+Y VL D                  +Q ++Y L  LY+NW GT+R+P    YAH+LA L+G ++ R      +++ + E+LFYL
Sbjct:  146 FNYEVRFFPPIDSVHLRIKYLNDHKDKLGGTKT-----FDGNTLYLPILLPNKMTVFISKAEDVELQIRILYKKKEEMRNCTQLYNILFDRVMKVLNYVKFDRKQFDPSR---------PKIIPLAKLEVWPGYVTAVD-----EYKGGLMLCCDVSHRILCQKTVLEMLVDLYQQNVEHYQESARKMLVGNIVLTRYNNRTYKINDICFDQNPTCQFEIKTGCTSYVEYYKQYHNINIKDVNQPLIYSIKKSRGI----------PAERENLQFCLIP-ELCYLTGLRDEVRSDNKLMREIATFTRVSPNQRQMALNKFYENVSNTPAAQEILNSWGLSLTNNSNKISGRQMDIEQIYFSKISVSAGRSAEFSKHAVTNEMLKVVHLSKWIIIHLRNYRQAATSLLDNMKQACESLGMNISNPTMISLDHDRIDAYIQALRRNITMNTQMVVCICHNRRDDRYAAIKKICCSEIPIPSQVINAKTLQNDLKI--RSVVQKIVLQMNCKLGGSLWTVKIPFKNVMICGIDSYHDPSNRGNSVAAFVASINSSYSQWYSKAVVQTKREEIVNGLSASFEIALKMYRKRNGKLPTNIIIYRDGIGDGQLYTCLNYE----IPQFEMVCGNR--IKISYIVVQKRINTRIFSGSGIHLENPLPGTVVDQHITKSNMYDFFLVSQLVRQGTVTPTHYVVLRD----------DCNYGPDIIQKLSYKLCFLYYNWAGTVRIPACCMYAHKLAYLIGQSIQRD-----VAEALSEKLFYL 867