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Planarian Educational Resource: Where Cutting Class is Required
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Nucleotide to Protein BLAST (blastx)
Request a New BLAST
Enter Nucleotide Query Sequence
Enter one or more queries in the top text box or use the browse button to upload a file from your local disk. The file may contain a single sequence or a list of sequences. In both cases, the data must be in
FASTA format
.
Show an Example Sequence
Enter FASTA sequence(s)
Enter query sequence(s) in the text area.
Choose Search Target
Choose from one of the protein BLAST databases listed below.
Protein BLAST Databases:
Dugesia dorotocephala Proteins
Four Planaria Species Proteins
Girardia sp. Proteins
Phagocata gracilis Proteins
Phagocata morgani Proteins
Select a Dataset
Advanced Options
General parameters
Max target sequences:
10
50
100
250
500
1000
5000
10000
20000
Select the maximum number of aligned sequences to display
e-Value (Expected Threshold)
Expected number of chance matches in a random model. This number should be give in a decimal format.
More Information
|
Expect value vedio tutorial
Word size:
3
6
The length of the seed that initiates an alignment
Max matches in a query range
Limit the number of matches to a query range. This option is useful if many strong matches to one part of a query may prevent BLAST from presenting weaker matches to another part of the query.
Scoring parameters
Matrix
PAM30
PAM70
PAM250
BLOSUM80
BLOSUM62
BLOSUM45
BLOSUM50
BLOSUM90
Assigns a score for aligning pairs of residues, and determines overall alignment score..
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Videos
Studying Planarian Regeneration
Through RNAi
Tools
Search Genes
Sequence Similarity Search
Nucleotide Query
BLASTn
BLASTx
Protein Query
BLASTp
tBLASTn
Primers
Glossary of Terms
Useful Links
Tutorials
Search Genes
: Search for planarian genes based on gene name or function
Sequence Similarity Search
: Search for planarian genes based on sequence similarity
Primer Design
: Design primer specific for a sequence of interest
Protocols
Planarian Maintenance
Observing Planarian Regeneration
Whole mount
in situ
Hybidization (WISH)
RNA Interference (RNAi)
Created by
Sánchez Lab
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