slit, Ddo_016416 (mRNA) Dugesia dorotocephala
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Overview
Analyses
This mRNA is derived from or has results from the following analyses
Properties
Homology
BLAST of slit vs. uniprot
Match: gi|33302576|sp|P24014|SLIT_DROME (RecName: Full=Protein slit; Short=dSlit; Contains: RecName: Full=Protein slit N-product; Contains: RecName: Full=Protein slit C-product; Flags: Precursor) HSP 1 Score: 742.265 bits (1915), Expect = 0.000e+0 Identity = 498/1549 (32.15%), Postives = 766/1549 (49.45%), Query Frame = 2
Query: 83 TIWINDPSEEVEI-----CPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXXQHLPDHLFSKLSKLQKLD------------------------LRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISEAI-TKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCANEENTCQPD---RKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXXTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGH-KCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDK-NPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNSLYF-GKDSHIEIHPPSRGFLMPKGNISLKFITNSSSGLILYQGFDKSYLVVNLFRGHIKISYNIGDKDIGPDGFSMDVVNDNKLHYLNIEIVGENLTMYLNGKVHFTLKSKYLMTNQYMALDTGIFIGGAPKKILNTAIQNHHFDNDSDFTGCIESLLINRKKIDFSKYMSKSVNVKPGCGDLISKTAQELPSSEVPTQDIEVPIKSTKSLPVKSTICSKPGVCSNNGVCHEVNEKNRKYKCVCKNGFVGRKCKK-----------RVSKCHGKTTRTFIKDPDTHDNCLSIKKFQYRVCKGTCSNNRMQADSNRNFYQRSTDNSLSPCCQPSKYTTHKIQFKCDNGRFYRRSVTLPRSCKCQGNCH 4588
++ I+ P V + CP+ C C +V+C HR +VP ++ RL L GN + VI ++DF +L L++LQL++N+I +IE +F +L++L +LRLN N ++ +P++ + + L +LD L NQ+ CL+EH F L L+ L L N L + I L LR LR+S N C+CHL WL+ FL+ + + P TR C +P +L ++ L EF+C+ + CP C C+ DGIV+C K LT++P LP +T EL L+ N IT L S ++ L++I L+ N + I A GL+QLT L L N ++ + F GL SL L L+ NEI C+ F + L LL L +N + +L + ++ ++L NP CDC + WL L + T A C P H ++SL+ F+C S +++ E C CP C C V+C+G GL +P I TT L+L+ N++ + + +LVKL+L NQ+ I+P+ F ++ ++E+ L NK+K ++N+ F L +L+ L L DN ISC+ SF+ +L L L +N +C+C L W +EW R G+ R C +P ++ + +L F C S E + C + CP C C T + CS QLKEIP IP T+ELYL+ N+++ ++ ++L +L L LS N IT ++ L L TL++S+NKLQC+ + A L+NL+VL L N +S +P +F DL +L +IALG NP +CDC +KW + + +++ GI+ CA PE+MK K I + + F+C + I AKC+AC + PCQN +C + +Y C C +HGK CE I+AC+G PCRN C + G + C C G+ G +C NIDDC G KC+N ATC+D V Y C+C GF G+FC++K +C + NPC N C + Y C C G+ G NC NIDDC+ ++C+N C D +N Y+C C Y G YCE + +S + S C HEC +GVC + C+C PGY+G +C+YL S+ F +S +E+ P P+ N+++ F + +G+++Y G D ++L V LF G I++SY++G+ + +S ++V D K H + + + +N T+ ++ + ++ ++ +N Y+ L T +F+GG P A +N N + F GC++ + IN K +DF + + PGC L+ QE E D IK P C + C N + Y+C CK+G GR C + S C + R + T ++C S + +Y C G C N CC K++ C N R Y +++ + R C C C+
Sbjct: 55 SVGIHIPGGGVGVITEARCPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLRLNNNRLKAIPENFVTSSASLLRLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRL-APYTR---CQSPSQLKGQNVADLHDQEFKCSGLTEHAPMECGAENSCPHPCRCA-DGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIE-TSGARCESPKRMHRRRIESLREEKFKC-SWDELRMKLSGE-CRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAAR---CGAPSKVRDVQIKDLPHSEFKC-----SSENSEGCLGDGY---------------CPPSCTCTGTVVRCSRNQLKEIPRGIPAETSELYLESNEIEQIHYERIRHLRSLTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCGLKWFSDWIKLDYVEPGIARCAEPEQMKDKLILSTPSSSFVCRGRVRND-ILAKCNACFEQPCQNQAQCVALPQREYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEE-GRFSCQCAPGYTGARCETNIDDCLGEIKCQNNATCIDGVESYKCECQPGFSGEFCDTKIQFCSPEFNPCANGAKCMDHFTH-YSCDCQAGFHGTNCTDNIDDCQNHMCQNGGTCVDGINDYQCRCPDDYTGKYCE------------------GHNMISMMYPQT------SPCQNHEC-KHGVCFQPNAQGSDYLCRCHPGYTGKWCEYLTSISFVHNNSFVELEPLR---TRPEANVTIVFSSAEQNGILMYDGQD-AHLAVELFNGRIRVSYDVGNHPVS-TMYSFEMVADGKYHAVELLAIKKNFTLRVDRGLARSIINE--GSNDYLKLTTPMFLGGLPVDPAQQAYKNWQIRNLTSFKGCMKEVWINHKLVDFGN-AQRQQKITPGCA-LLEGEQQEEEDDEQDFMDETPHIKEEPVDPCLENKCRRGSRCVPNSNARD------GYQCKCKHGQRGRYCDQGEGSTEPPTVTAASTCRKEQVREYY----TENDCRSRQPLKYAKCVGGCGNQ---------------------CCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKKCY 1504 HSP 2 Score: 742.265 bits (1915), Expect = 0.000e+0 Identity = 498/1549 (32.15%), Postives = 766/1549 (49.45%), Query Frame = -2
Query: 5043 TIWINDPSEEVEI-----CPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXIQHLPDHLFSKLSKLQKLD------------------------LRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISEAI-TKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCANEENTCQPD---RKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXLTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGH-KCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDK-NPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNSLYF-GKDSHIEIHPPSRGFLMPKGNISLKFITNSSSGLILYQGFDKSYLVVNLFRGHIKISYNIGDKDIGPDGFSMDVVNDNKLHYLNIEIVGENLTMYLNGKVHFTLKSKYLMTNQYMALDTGIFIGGAPKKILNTAIQNHHFDNDSDFTGCIESLLINRKKIDFSKYMSKSVNVKPGCGDLISKTAQELPSSEVPTQDIEVPIKSTKSLPVKSTICSKPGVCSNNGVCHEVNEKNRKYKCVCKNGFVGRKCKK-----------RVSKCHGKTTRTFIKDPDTHDNCLSIKKFQYRVCKGTCSNNRMQADSNRNFYQRSTDNSLSPCCQPSKYTTHKIQFKCDNGRFYRRSVTLPRSCKCQGNCH 9548
++ I+ P V + CP+ C C +V+C HR +VP ++ RL L GN + VI ++DF +L L++LQL++N+I +IE +F +L++L +LRLN N ++ +P++ + + L +LD L NQ+ CL+EH F L L+ L L N L + I L LR LR+S N C+CHL WL+ FL+ + + P TR C +P +L ++ L EF+C+ + CP C C+ DGIV+C K LT++P LP +T EL L+ N IT L S ++ L++I L+ N + I A GL+QLT L L N ++ + F GL SL L L+ NEI C+ F + L LL L +N + +L + ++ ++L NP CDC + WL L + T A C P H ++SL+ F+C S +++ E C CP C C V+C+G GL +P I TT L+L+ N++ + + +LVKL+L NQ+ I+P+ F ++ ++E+ L NK+K ++N+ F L +L+ L L DN ISC+ SF+ +L L L +N +C+C L W +EW R G+ R C +P ++ + +L F C S E + C + CP C C T + CS QLKEIP IP T+ELYL+ N+++ ++ ++L +L L LS N IT ++ L L TL++S+NKLQC+ + A L+NL+VL L N +S +P +F DL +L +IALG NP +CDC +KW + + +++ GI+ CA PE+MK K I + + F+C + I AKC+AC + PCQN +C + +Y C C +HGK CE I+AC+G PCRN C + G + C C G+ G +C NIDDC G KC+N ATC+D V Y C+C GF G+FC++K +C + NPC N C + Y C C G+ G NC NIDDC+ ++C+N C D +N Y+C C Y G YCE + +S + S C HEC +GVC + C+C PGY+G +C+YL S+ F +S +E+ P P+ N+++ F + +G+++Y G D ++L V LF G I++SY++G+ + +S ++V D K H + + + +N T+ ++ + ++ ++ +N Y+ L T +F+GG P A +N N + F GC++ + IN K +DF + + PGC L+ QE E D IK P C + C N + Y+C CK+G GR C + S C + R + T ++C S + +Y C G C N CC K++ C N R Y +++ + R C C C+
Sbjct: 55 SVGIHIPGGGVGVITEARCPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLRLNNNRLKAIPENFVTSSASLLRLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRL-APYTR---CQSPSQLKGQNVADLHDQEFKCSGLTEHAPMECGAENSCPHPCRCA-DGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIE-TSGARCESPKRMHRRRIESLREEKFKC-SWDELRMKLSGE-CRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAAR---CGAPSKVRDVQIKDLPHSEFKC-----SSENSEGCLGDGY---------------CPPSCTCTGTVVRCSRNQLKEIPRGIPAETSELYLESNEIEQIHYERIRHLRSLTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCGLKWFSDWIKLDYVEPGIARCAEPEQMKDKLILSTPSSSFVCRGRVRND-ILAKCNACFEQPCQNQAQCVALPQREYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEE-GRFSCQCAPGYTGARCETNIDDCLGEIKCQNNATCIDGVESYKCECQPGFSGEFCDTKIQFCSPEFNPCANGAKCMDHFTH-YSCDCQAGFHGTNCTDNIDDCQNHMCQNGGTCVDGINDYQCRCPDDYTGKYCE------------------GHNMISMMYPQT------SPCQNHEC-KHGVCFQPNAQGSDYLCRCHPGYTGKWCEYLTSISFVHNNSFVELEPLR---TRPEANVTIVFSSAEQNGILMYDGQD-AHLAVELFNGRIRVSYDVGNHPVS-TMYSFEMVADGKYHAVELLAIKKNFTLRVDRGLARSIINE--GSNDYLKLTTPMFLGGLPVDPAQQAYKNWQIRNLTSFKGCMKEVWINHKLVDFGN-AQRQQKITPGCA-LLEGEQQEEEDDEQDFMDETPHIKEEPVDPCLENKCRRGSRCVPNSNARD------GYQCKCKHGQRGRYCDQGEGSTEPPTVTAASTCRKEQVREYY----TENDCRSRQPLKYAKCVGGCGNQ---------------------CCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKKCY 1504
BLAST of slit vs. uniprot
Match: gi|45477220|sp|O88280|SLIT3_RAT (RecName: Full=Slit homolog 3 protein; Short=Slit-3; AltName: Full=Multiple epidermal growth factor-like domains protein 5; Short=Multiple EGF-like domains protein 5; Flags: Precursor) HSP 1 Score: 736.487 bits (1900), Expect = 0.000e+0 Identity = 503/1584 (31.76%), Postives = 775/1584 (48.93%), Query Frame = 2
Query: 122 CPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXXQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISEA----------------------ITKLKHL---RELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCA---NEENTCQPDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXXTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLK------------------------CVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNE--SSFKNLINLKTLV-LSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGN-YECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYC-HDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNSLYF-GKDSHIEIHPPSRGFLMPKGNISLKFITNSSSGLILYQGFDKSYLVVNLFRGHIKISYNIGDKDIGPDG--FSMDVVNDNKLHYLNIEIVGENLTMYLNGKVHFTLKSKYLMTNQYMALDTGIFIGGAPKKILNTAIQNHHFDNDSDFTGCIESLLINRKKIDFSKYMSKSVNVKPGCGDLISKTAQELPSSEVPTQDIEVPIKSTKSL-------PVKSTICSKPGVCSNNG--------------VCHEVNEKN---RKYKC---------------VCKNGFVGRKCKKRVSKCHGKTTRTFIKDPDTHDNCLSIKKFQYRVCKGTCSNNRMQADSNRNFYQRSTDNSLSPCCQPSKYTTHKIQFKCDNGRFYRRSVTLPRSCKCQ 4576
CP C C SV+C R VP + RL+L N I I K DF L L+VL L +N++S IE GAF +L L +LRLN+N +Q LP+ LF KL +LDL ENQ+Q + F + +K L L+ N + I + +T H+ R LR+ SN L+C+CHL WL+ +L+ + I + +C+ P L ++ + E+ C +E C + CP C+CS + IV+C GK LT IP +LP +E+ L+ NSI + G +YK LK+I +++N + DI P AF GL+ LT+L L N + I + F+GL SL L L+ N+I CL TF+ ++ L LL L +NKL + + + PL ++ L+L NP CDC + WL L+ + T A C P + +++ F+C+ D + E C ++CP KC C IV+CS LS +P + TT L L+ NDI L+ + NL K++LSNN+I+ ++ F + ++E+ L+ N+L+ CVNN+TF L + +L L DN I+ I +F +L + L +N +C+C + WL W R + G+ P CQ P LK P+ ++ + F CEGN EE S C P+CP +C C T + CS L +P +P + TELYL+ N L + + S+F+ L TL+ LS N I+ + + + +L TL+LS+N+L+CI AF L +L+VL L ND+S++P +F DLT+L+++ALG NP HCDC+++WL+++ + + GI+ C+SPE M + + + F C D I AKC+AC+ +PC+NNG CS Y C CP + GK C IN C PC++GG C +++ + + C CP GF G +C N DDC + CEN ATCVD +N Y+C CP + G+ C+ YC + N C++ C L + ++C C G+ GK CE + DDC + C++ A+C D VNGY CIC G+ G +CE P P L + S C +EC N C+ + +Q+ C+C PG++GP C+ L ++ F GKDS++E+ + + P+ NISL+ T+ +G++LY+G D L + L++GH+++ Y D P +S++ VND + H + + ++ + L + ++ +L L + +++ +++GG P +A++ F GCI + IN + DF +S+ V PGC S E + E T L P CS G C G +C + N+ +KC +C+ GF G C++ + C G+ R I+ + +C + K VC+G C S CCQP + K F+C +G + V C C+
Sbjct: 34 CPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFTGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGVTGVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTI----GQFTLCMAPVHLRGFSVADVQKKEYVCPGPHSEAPACNANSLSCPSACSCSNN-IVDCRGKGLTEIPANLPEGIVEIRLEQNSIKSIPAGAFIQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIPKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIE-TSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRNRFSSE-CFMDLVCPEKCRCEGTIVDCSNQKLSRIPSHLPEYTTDLRLNDNDIAVLEATGIFKKLPNLRKINLSNNRIKEVREGAFDGAAGVQELMLTGNQLETMHGRMFRGLSGLKTLMLRSNLISCVNNDTFAGLSSVRLLSLYDNRITTISPGAFTTLVSLSTINLLSNPFNCNCHMAWLGRWLRKRRIVSGN---PRCQKPFFLKEIPIQDVAIQDFTCEGN----EENS----------------CQLSPRCPEQCTCVETVVRCSNRGLHTLPKGMPKDVTELYLEGNHLTAVPKELSTFRQL----TLIDLSNNSISMLTNHTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGINPLHCDCSLRWLSEWIKAGYKEPGIARCSSPESMADRLLLTTPTHRFQCKGPV-DINIVAKCNACLSSPCKNNGTCSQDPVEQYRCTCPYSYKGKDCTVPINTCVQNPCQHGGTCHLSESHRDGFSCSCPLGFEGQRCEINPDDCEDNDCENSATCVDGINNYACVCPPNYTGELCDEVIDYCVPEMNLCQHEAKCISL-DKGFRCECVPGYSGKLCETDNDDCVAHKCRHGAQCVDAVNGYTCICPQGFSGLFCEHPP--PMVL------------------------LQTSPCDQYECQNGAQCI---VVQQEPTCRCPPGFAGPRCEKLITVNFVGKDSYVEL---ASAKVRPQANISLQVATDKDNGILLYKG-DNDPLALELYQGHVRLVY---DSLSSPPTTVYSVETVNDGQFHSVELVMLNQTLNLVVDKGAPKSLGK--LQKQPAVGINSPLYLGGIPTSTGLSALRQGADRPLGGFHGCIHEVRINNELQDFKALPPQSLGVSPGCKSCTVCRHGLCRSVEKDSVVCECHPGWTGPLCDQEAQDPCLGHSCSH-GTCVATGNSYVCKCAEGYEGPLCDQKNDSANACSAFKCHHGQCHISDRGEPYCLCQPGFSGNHCEQE-NPCLGEIVREAIRRQKDYASCATASKVPIMVCRGGCG---------------------SQCCQPIRSKRRKYVFQCTDGSSFVEEVERHLECGCR 1520 HSP 2 Score: 736.487 bits (1900), Expect = 0.000e+0 Identity = 503/1584 (31.76%), Postives = 775/1584 (48.93%), Query Frame = -2
Query: 5055 CPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXIQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISEA----------------------ITKLKHL---RELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCA---NEENTCQPDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXLTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLK------------------------CVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNE--SSFKNLINLKTLV-LSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGN-YECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYC-HDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNSLYF-GKDSHIEIHPPSRGFLMPKGNISLKFITNSSSGLILYQGFDKSYLVVNLFRGHIKISYNIGDKDIGPDG--FSMDVVNDNKLHYLNIEIVGENLTMYLNGKVHFTLKSKYLMTNQYMALDTGIFIGGAPKKILNTAIQNHHFDNDSDFTGCIESLLINRKKIDFSKYMSKSVNVKPGCGDLISKTAQELPSSEVPTQDIEVPIKSTKSL-------PVKSTICSKPGVCSNNG--------------VCHEVNEKN---RKYKC---------------VCKNGFVGRKCKKRVSKCHGKTTRTFIKDPDTHDNCLSIKKFQYRVCKGTCSNNRMQADSNRNFYQRSTDNSLSPCCQPSKYTTHKIQFKCDNGRFYRRSVTLPRSCKCQ 9509
CP C C SV+C R VP + RL+L N I I K DF L L+VL L +N++S IE GAF +L L +LRLN+N +Q LP+ LF KL +LDL ENQ+Q + F + +K L L+ N + I + +T H+ R LR+ SN L+C+CHL WL+ +L+ + I + +C+ P L ++ + E+ C +E C + CP C+CS + IV+C GK LT IP +LP +E+ L+ NSI + G +YK LK+I +++N + DI P AF GL+ LT+L L N + I + F+GL SL L L+ N+I CL TF+ ++ L LL L +NKL + + + PL ++ L+L NP CDC + WL L+ + T A C P + +++ F+C+ D + E C ++CP KC C IV+CS LS +P + TT L L+ NDI L+ + NL K++LSNN+I+ ++ F + ++E+ L+ N+L+ CVNN+TF L + +L L DN I+ I +F +L + L +N +C+C + WL W R + G+ P CQ P LK P+ ++ + F CEGN EE S C P+CP +C C T + CS L +P +P + TELYL+ N L + + S+F+ L TL+ LS N I+ + + + +L TL+LS+N+L+CI AF L +L+VL L ND+S++P +F DLT+L+++ALG NP HCDC+++WL+++ + + GI+ C+SPE M + + + F C D I AKC+AC+ +PC+NNG CS Y C CP + GK C IN C PC++GG C +++ + + C CP GF G +C N DDC + CEN ATCVD +N Y+C CP + G+ C+ YC + N C++ C L + ++C C G+ GK CE + DDC + C++ A+C D VNGY CIC G+ G +CE P P L + S C +EC N C+ + +Q+ C+C PG++GP C+ L ++ F GKDS++E+ + + P+ NISL+ T+ +G++LY+G D L + L++GH+++ Y D P +S++ VND + H + + ++ + L + ++ +L L + +++ +++GG P +A++ F GCI + IN + DF +S+ V PGC S E + E T L P CS G C G +C + N+ +KC +C+ GF G C++ + C G+ R I+ + +C + K VC+G C S CCQP + K F+C +G + V C C+
Sbjct: 34 CPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFTGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGVTGVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTI----GQFTLCMAPVHLRGFSVADVQKKEYVCPGPHSEAPACNANSLSCPSACSCSNN-IVDCRGKGLTEIPANLPEGIVEIRLEQNSIKSIPAGAFIQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIPKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIE-TSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRNRFSSE-CFMDLVCPEKCRCEGTIVDCSNQKLSRIPSHLPEYTTDLRLNDNDIAVLEATGIFKKLPNLRKINLSNNRIKEVREGAFDGAAGVQELMLTGNQLETMHGRMFRGLSGLKTLMLRSNLISCVNNDTFAGLSSVRLLSLYDNRITTISPGAFTTLVSLSTINLLSNPFNCNCHMAWLGRWLRKRRIVSGN---PRCQKPFFLKEIPIQDVAIQDFTCEGN----EENS----------------CQLSPRCPEQCTCVETVVRCSNRGLHTLPKGMPKDVTELYLEGNHLTAVPKELSTFRQL----TLIDLSNNSISMLTNHTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGINPLHCDCSLRWLSEWIKAGYKEPGIARCSSPESMADRLLLTTPTHRFQCKGPV-DINIVAKCNACLSSPCKNNGTCSQDPVEQYRCTCPYSYKGKDCTVPINTCVQNPCQHGGTCHLSESHRDGFSCSCPLGFEGQRCEINPDDCEDNDCENSATCVDGINNYACVCPPNYTGELCDEVIDYCVPEMNLCQHEAKCISL-DKGFRCECVPGYSGKLCETDNDDCVAHKCRHGAQCVDAVNGYTCICPQGFSGLFCEHPP--PMVL------------------------LQTSPCDQYECQNGAQCI---VVQQEPTCRCPPGFAGPRCEKLITVNFVGKDSYVEL---ASAKVRPQANISLQVATDKDNGILLYKG-DNDPLALELYQGHVRLVY---DSLSSPPTTVYSVETVNDGQFHSVELVMLNQTLNLVVDKGAPKSLGK--LQKQPAVGINSPLYLGGIPTSTGLSALRQGADRPLGGFHGCIHEVRINNELQDFKALPPQSLGVSPGCKSCTVCRHGLCRSVEKDSVVCECHPGWTGPLCDQEAQDPCLGHSCSH-GTCVATGNSYVCKCAEGYEGPLCDQKNDSANACSAFKCHHGQCHISDRGEPYCLCQPGFSGNHCEQE-NPCLGEIVREAIRRQKDYASCATASKVPIMVCRGGCG---------------------SQCCQPIRSKRRKYVFQCTDGSSFVEEVERHLECGCR 1520 HSP 3 Score: 286.96 bits (733), Expect = 1.964e-76 Identity = 211/714 (29.55%), Postives = 321/714 (44.96%), Query Frame = 2
Query: 773 PDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXXTQNGMVDIEPGAFIGLQQLTALYLNMNNLRT------------------------IRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINL------------------------SSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESS-FKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVS------------------------TIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDT 2695
P CP +CTCS V+C G L +P+ +P N LDL N+IT + + + K L+ + L N + IE GAF L+QL L LN N L+ I R AF G++ + L L NN I C+ G F ++ L +L L+ N +S + + K+R L L N LYCDC + WL L+ + C+ P G +V +Q + C + + + CP CSCS+ IV+C G GL+ +P + + L N IK++ + Q L ++D+S NQI +I PD F L + L ++NK+ C+ TF+ L L +L L DN + I F +++ L L N CDC LKWL+++ ++N R C SP L + + K F C G++ + FS C +D+ CP KC+CE T +DCS +L IP+ +P TT+L L+ ND+ L + FK L NL+ + LS N I E+ + ++ L+L+ N+L+ +H F+ LS LK L+L+SN +S TI AF L +L+ I L NPF+C+C++ WL ++ R + +G C P +K I +F C E++
Sbjct: 29 PPAAACPTKCTCSA-ASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFTGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGVTGVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTIGQ-FTLCMAPVHLRGFSVADVQKKEYVCPGPHSEAPACNANS----LSCPSACSCSNNIVDCRGKGLTEIPANLPEGIVEIRLEQNSIKSIPAGAFI-QYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIPKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGAR---CSSPRRLANKRISQIKSKKFRCSGSEDYRNR--------FSSECFMDL------VCPEKCRCEGTIVDCSNQKLSRIPSHLPEYTTDLRLNDNDIAVLEATGIFKKLPNLRKINLSNNRIKEVREGAFDGAAGVQELMLTGNQLETMHGRMFRGLSGLKTLMLRSNLISCVNNDTFAGLSSVRLLSLYDNRITTISPGAFTTLVSLSTINLLSNPFNCNCHMAWLGRWLRKRRIVSGNPRCQKPFFLKEIPIQDVAIQDFTCEGNEENS 718 HSP 4 Score: 286.96 bits (733), Expect = 1.964e-76 Identity = 211/714 (29.55%), Postives = 321/714 (44.96%), Query Frame = -2
Query: 6936 PDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXLTQNGMVDIEPGAFIGLQQLTALYLNMNNLRT------------------------IRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINL------------------------SSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESS-FKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVS------------------------TIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDT 8858
P CP +CTCS V+C G L +P+ +P N LDL N+IT + + + K L+ + L N + IE GAF L+QL L LN N L+ I R AF G++ + L L NN I C+ G F ++ L +L L+ N +S + + K+R L L N LYCDC + WL L+ + C+ P G +V +Q + C + + + CP CSCS+ IV+C G GL+ +P + + L N IK++ + Q L ++D+S NQI +I PD F L + L ++NK+ C+ TF+ L L +L L DN + I F +++ L L N CDC LKWL+++ ++N R C SP L + + K F C G++ + FS C +D+ CP KC+CE T +DCS +L IP+ +P TT+L L+ ND+ L + FK L NL+ + LS N I E+ + ++ L+L+ N+L+ +H F+ LS LK L+L+SN +S TI AF L +L+ I L NPF+C+C++ WL ++ R + +G C P +K I +F C E++
Sbjct: 29 PPAAACPTKCTCSA-ASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFTGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGVTGVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTIGQ-FTLCMAPVHLRGFSVADVQKKEYVCPGPHSEAPACNANS----LSCPSACSCSNNIVDCRGKGLTEIPANLPEGIVEIRLEQNSIKSIPAGAFI-QYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIPKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGAR---CSSPRRLANKRISQIKSKKFRCSGSEDYRNR--------FSSECFMDL------VCPEKCRCEGTIVDCSNQKLSRIPSHLPEYTTDLRLNDNDIAVLEATGIFKKLPNLRKINLSNNRIKEVREGAFDGAAGVQELMLTGNQLETMHGRMFRGLSGLKTLMLRSNLISCVNNDTFAGLSSVRLLSLYDNRITTISPGAFTTLVSLSTINLLSNPFNCNCHMAWLGRWLRKRRIVSGNPRCQKPFFLKEIPIQDVAIQDFTCEGNEENS 718 HSP 5 Score: 211.075 bits (536), Expect = 4.047e-53 Identity = 141/440 (32.05%), Postives = 219/440 (49.77%), Query Frame = 2
Query: 119 ICPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFF-KLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXXQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISE-AITKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQC-ANEENTCQPDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXXTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDI 1429
+CP+ C+CE V+C ++ +P+ L E TT L L N I V+ + F KL L+ + LSNNRI + GAFD + +L L N ++ + +F LS L+ L LR N + C+N F+ L ++ L L N++ IS A T L L + + SN +CNCH+ WL +L+ K I + R C P L +IPI ++ +F C NEEN+CQ + CP+QCTC + +V C+ + L +P+ +P + EL L+ N +T + P +QLT + L+ N++ + + F+ +S L L L N + C+P F G++ L +L L N +S++ E L L +L L NPL+CDC + WL + +K +A C P S + + + FQC DI
Sbjct: 504 VCPEKCRCEGTIVDCSNQKLSRIPSHLPEYTTDLRLNDNDIAVLEATGIFKKLPNLRKINLSNNRIKEVREGAFDGAAGVQELMLTGNQLETMHGRMFRGLSGLKTLMLRSNLISCVNNDTFAGLSSVRLLSLYDNRITTISPGAFTTLVSLSTINLLSNPFNCNCHMAWLGRWLR-KRRIVSGNPR---CQKPFFLKEIPIQDVAIQDFTCEGNEENSCQLSPR-CPEQCTCVET-VVRCSNRGLHTLPKGMPKDVTELYLEGNHLTAV-------------------------PKELSTFRQLTLIDLSNNSISMLTNHTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGINPLHCDCSLRWLSEWIK-AGYKEPGIARCSSPESMADRLLLTTPTHRFQCKGPVDI 911 HSP 6 Score: 211.075 bits (536), Expect = 4.047e-53 Identity = 141/440 (32.05%), Postives = 219/440 (49.77%), Query Frame = -2
Query: 8202 ICPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFF-KLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXIQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISE-AITKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQC-ANEENTCQPDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXLTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDI 9512
+CP+ C+CE V+C ++ +P+ L E TT L L N I V+ + F KL L+ + LSNNRI + GAFD + +L L N ++ + +F LS L+ L LR N + C+N F+ L ++ L L N++ IS A T L L + + SN +CNCH+ WL +L+ K I + R C P L +IPI ++ +F C NEEN+CQ + CP+QCTC + +V C+ + L +P+ +P + EL L+ N +T + P +QLT + L+ N++ + + F+ +S L L L N + C+P F G++ L +L L N +S++ E L L +L L NPL+CDC + WL + +K +A C P S + + + FQC DI
Sbjct: 504 VCPEKCRCEGTIVDCSNQKLSRIPSHLPEYTTDLRLNDNDIAVLEATGIFKKLPNLRKINLSNNRIKEVREGAFDGAAGVQELMLTGNQLETMHGRMFRGLSGLKTLMLRSNLISCVNNDTFAGLSSVRLLSLYDNRITTISPGAFTTLVSLSTINLLSNPFNCNCHMAWLGRWLR-KRRIVSGNPR---CQKPFFLKEIPIQDVAIQDFTCEGNEENSCQLSPR-CPEQCTCVET-VVRCSNRGLHTLPKGMPKDVTELYLEGNHLTAV-------------------------PKELSTFRQLTLIDLSNNSISMLTNHTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGINPLHCDCSLRWLSEWIK-AGYKEPGIARCSSPESMADRLLLTTPTHRFQCKGPVDI 911
BLAST of slit vs. uniprot
Match: gi|341942041|sp|Q9WVB4|SLIT3_MOUSE (RecName: Full=Slit homolog 3 protein; Short=Slit-3; Short=Slit3; Flags: Precursor) HSP 1 Score: 732.635 bits (1890), Expect = 0.000e+0 Identity = 496/1584 (31.31%), Postives = 772/1584 (48.74%), Query Frame = 2
Query: 122 CPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXXQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISEA----------------------ITKLKHL---RELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCA---NEENTCQPDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXXTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLK------------------------CVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNE--SSFKNLINLKTLV-LSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGN-YECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYC-HDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNSLYF-GKDSHIEIHPPSRGFLMPKGNISLKFITNSSSGLILYQGFDKSYLVVNLFRGHIKISYNIGDKDIGPDG--FSMDVVNDNKLHYLNIEIVGENLTMYLNGKVHFTLKSKYLMTNQYMALDTGIFIGGAPKKILNTAIQNHHFDNDSDFTGCIESLLINRKKIDFSKYMSKSVNVKPGCGDLI-------------SKTAQELPSSEVPTQDIEV-------PIKSTKSLPVKSTICSKPGVCSNNGVCHEVNEKN---RKYKC---------------VCKNGFVGRKCKKRVSKCHGKTTRTFIKDPDTHDNCLSIKKFQYRVCKGTCSNNRMQADSNRNFYQRSTDNSLSPCCQPSKYTTHKIQFKCDNGRFYRRSVTLPRSCKCQG 4579
CP C C SV+C R VP + RL+L N I I K DF L L+VL L +N++S IE GAF +L L +LRLN+N +Q LP+ LF KL +LDL ENQ+Q + F + +K L L+ N + I + +T H+ R LR+ SN L+C+CHL WL+ +L+ + I + +C+ P L ++ + E+ C +E C + CP C+CS + IV+C GK LT IP +LP +E+ L+ NSI + G ++YK LK+I +++N + DI P AF GL+ LT+L L N + I + F+GL SL L L+ N+I CL TF+ ++ L LL L +NKL + + + PL ++ L+L NP CDC + WL L+ + T A C P + +++ F+C+ D + E C ++CP KC C IV+CS L+ +P + TT L L+ NDI L+ + NL K++LSNN+I+ ++ F + ++E+ L+ N+L+ CV+N+TF L + +L L DN I+ I +F +L + L +N +C+C + WL W R + G+ P CQ P LK P+ ++ + F C+GN EE+S C P+CP +C C T + CS L +P +P + TELYL+ N L + + S+F+ L TL+ LS N I+ + + + +L TL+LS+N+L+CI AF L +L+VL L ND+S++P +F DLT+L+++ALG NP HCDC+++WL+++ + + GI+ C+SPE M + + + F C D I AKC+AC+ +PC+NNG CS Y C CP + GK C IN C PC +GG C ++N + + C CP GF G +C N DDC + CEN ATCVD +N Y+C CP + G+ C+ YC + N C++ C L + ++C C G+ GK CE N DDC + C++ A+C D VNGY CIC G+ G +CE P P L + S C +EC N C+ + +Q+ C+C PG++GP C+ L ++ F GKDS++E+ + + P+ NISL+ T+ +G++LY+G D L + L++GH+++ Y D P +S++ VND + H + + ++ + L + ++ +L L + ++ +++GG P +A++ F GCI + IN + DF +S+ V PGC S + P P D E + + + K +C + N+ +KC +C+ GF G C++ + C G+ R I+ + +C + K C+G C S CCQP + K F+C +G + V C C+
Sbjct: 34 CPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSIIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGVTGVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTI----GQFTLCMAPVHLRGFSVADVQKKEYVCPGPHSEAPACNANSLSCPSACSCSNN-IVDCRGKGLTEIPANLPEGIVEIRLEQNSIKSIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIPKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFVPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIE-TSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRNRFSSE-CFMDLVCPEKCRCEGTIVDCSNQKLARIPSHLPEYTTDLRLNDNDISVLEATGIFKKLPNLRKINLSNNRIKEVREGAFDGAAGVQELMLTGNQLETMHGRMFRGLSSLKTLMLRSNLISCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHMAWLGRWLRKRRIVSGN---PRCQKPFFLKEIPIQDVAIQDFTCDGN----EESS----------------CQLSPRCPEQCTCVETVVRCSNRGLHALPKGMPKDVTELYLEGNHLTAVPKELSAFRQL----TLIDLSNNSISMLTNHTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPESMADRLLLTTPTHRFQCKGPV-DINIVAKCNACLSSPCKNNGTCSQDPVEQYRCTCPYSYKGKDCTVPINTCVQNPCEHGGTCHLSENLRDGFSCSCPLGFEGQRCEINPDDCEDNDCENSATCVDGINNYACLCPPNYTGELCDEVIDYCVPEMNLCQHEAKCISL-DKGFRCECVPGYSGKLCETNNDDCVAHKCRHGAQCVDEVNGYTCICPQGFSGLFCEHPP--PMVL------------------------LQTSPCDQYECQNGAQCI---VVQQEPTCRCPPGFAGPRCEKLITVNFVGKDSYVEL---ASAKVRPQANISLQVATDKDNGILLYKG-DNDPLALELYQGHVRLVY---DSLSSPPTTVYSVETVNDGQFHSVELVMLNQTLNLVVDKGAPKSLGK--LQKQPAVGSNSPLYLGGIPTSTGLSALRQGADRPLGGFHGCIHEVRINNELQDFKALPPQSLGVSPGCKSCTVCRHGLCRSVEKDSVVCECHPGWTGPLCDQEARDPCLGHSCRHGTCMATGDSYVCKCAEGYGGALCDQKNDSASACSAFKCHHGQCHISDRGEPYCLCQPGFSGHHCEQE-NPCMGEIVREAIRRQKDYASCATASKVPIMECRGGCG---------------------SQCCQPIRSKRRKYVFQCTDGSSFVEEVERHLECGCRA 1521 HSP 2 Score: 732.635 bits (1890), Expect = 0.000e+0 Identity = 496/1584 (31.31%), Postives = 772/1584 (48.74%), Query Frame = -2
Query: 5052 CPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXIQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISEA----------------------ITKLKHL---RELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCA---NEENTCQPDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXLTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLK------------------------CVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNE--SSFKNLINLKTLV-LSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGN-YECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYC-HDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNSLYF-GKDSHIEIHPPSRGFLMPKGNISLKFITNSSSGLILYQGFDKSYLVVNLFRGHIKISYNIGDKDIGPDG--FSMDVVNDNKLHYLNIEIVGENLTMYLNGKVHFTLKSKYLMTNQYMALDTGIFIGGAPKKILNTAIQNHHFDNDSDFTGCIESLLINRKKIDFSKYMSKSVNVKPGCGDLI-------------SKTAQELPSSEVPTQDIEV-------PIKSTKSLPVKSTICSKPGVCSNNGVCHEVNEKN---RKYKC---------------VCKNGFVGRKCKKRVSKCHGKTTRTFIKDPDTHDNCLSIKKFQYRVCKGTCSNNRMQADSNRNFYQRSTDNSLSPCCQPSKYTTHKIQFKCDNGRFYRRSVTLPRSCKCQG 9509
CP C C SV+C R VP + RL+L N I I K DF L L+VL L +N++S IE GAF +L L +LRLN+N +Q LP+ LF KL +LDL ENQ+Q + F + +K L L+ N + I + +T H+ R LR+ SN L+C+CHL WL+ +L+ + I + +C+ P L ++ + E+ C +E C + CP C+CS + IV+C GK LT IP +LP +E+ L+ NSI + G ++YK LK+I +++N + DI P AF GL+ LT+L L N + I + F+GL SL L L+ N+I CL TF+ ++ L LL L +NKL + + + PL ++ L+L NP CDC + WL L+ + T A C P + +++ F+C+ D + E C ++CP KC C IV+CS L+ +P + TT L L+ NDI L+ + NL K++LSNN+I+ ++ F + ++E+ L+ N+L+ CV+N+TF L + +L L DN I+ I +F +L + L +N +C+C + WL W R + G+ P CQ P LK P+ ++ + F C+GN EE+S C P+CP +C C T + CS L +P +P + TELYL+ N L + + S+F+ L TL+ LS N I+ + + + +L TL+LS+N+L+CI AF L +L+VL L ND+S++P +F DLT+L+++ALG NP HCDC+++WL+++ + + GI+ C+SPE M + + + F C D I AKC+AC+ +PC+NNG CS Y C CP + GK C IN C PC +GG C ++N + + C CP GF G +C N DDC + CEN ATCVD +N Y+C CP + G+ C+ YC + N C++ C L + ++C C G+ GK CE N DDC + C++ A+C D VNGY CIC G+ G +CE P P L + S C +EC N C+ + +Q+ C+C PG++GP C+ L ++ F GKDS++E+ + + P+ NISL+ T+ +G++LY+G D L + L++GH+++ Y D P +S++ VND + H + + ++ + L + ++ +L L + ++ +++GG P +A++ F GCI + IN + DF +S+ V PGC S + P P D E + + + K +C + N+ +KC +C+ GF G C++ + C G+ R I+ + +C + K C+G C S CCQP + K F+C +G + V C C+
Sbjct: 34 CPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSIIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGVTGVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTI----GQFTLCMAPVHLRGFSVADVQKKEYVCPGPHSEAPACNANSLSCPSACSCSNN-IVDCRGKGLTEIPANLPEGIVEIRLEQNSIKSIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIPKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFVPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIE-TSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRNRFSSE-CFMDLVCPEKCRCEGTIVDCSNQKLARIPSHLPEYTTDLRLNDNDISVLEATGIFKKLPNLRKINLSNNRIKEVREGAFDGAAGVQELMLTGNQLETMHGRMFRGLSSLKTLMLRSNLISCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHMAWLGRWLRKRRIVSGN---PRCQKPFFLKEIPIQDVAIQDFTCDGN----EESS----------------CQLSPRCPEQCTCVETVVRCSNRGLHALPKGMPKDVTELYLEGNHLTAVPKELSAFRQL----TLIDLSNNSISMLTNHTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPESMADRLLLTTPTHRFQCKGPV-DINIVAKCNACLSSPCKNNGTCSQDPVEQYRCTCPYSYKGKDCTVPINTCVQNPCEHGGTCHLSENLRDGFSCSCPLGFEGQRCEINPDDCEDNDCENSATCVDGINNYACLCPPNYTGELCDEVIDYCVPEMNLCQHEAKCISL-DKGFRCECVPGYSGKLCETNNDDCVAHKCRHGAQCVDEVNGYTCICPQGFSGLFCEHPP--PMVL------------------------LQTSPCDQYECQNGAQCI---VVQQEPTCRCPPGFAGPRCEKLITVNFVGKDSYVEL---ASAKVRPQANISLQVATDKDNGILLYKG-DNDPLALELYQGHVRLVY---DSLSSPPTTVYSVETVNDGQFHSVELVMLNQTLNLVVDKGAPKSLGK--LQKQPAVGSNSPLYLGGIPTSTGLSALRQGADRPLGGFHGCIHEVRINNELQDFKALPPQSLGVSPGCKSCTVCRHGLCRSVEKDSVVCECHPGWTGPLCDQEARDPCLGHSCRHGTCMATGDSYVCKCAEGYGGALCDQKNDSASACSAFKCHHGQCHISDRGEPYCLCQPGFSGHHCEQE-NPCMGEIVREAIRRQKDYASCATASKVPIMECRGGCG---------------------SQCCQPIRSKRRKYVFQCTDGSSFVEEVERHLECGCRA 1521 HSP 3 Score: 286.189 bits (731), Expect = 3.023e-76 Identity = 211/714 (29.55%), Postives = 321/714 (44.96%), Query Frame = 2
Query: 773 PDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXXTQNGMVDIEPGAFIGLQQLTALYLNMNNLRT------------------------IRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINL------------------------SSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESS-FKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVS------------------------TIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDT 2695
P CP +CTCS V+C G L +P+ +P N LDL N+IT + + + K L+ + L N + IE GAF L+QL L LN N L+ I R AF G++ + L L NN I C+ G F ++ L +L L+ N +S + + K+R L L N LYCDC + WL L+ + C+ P G +V +Q + C + + + CP CSCS+ IV+C G GL+ +P + + L N IK++ + Q L ++D+S NQI +I PD F L + L ++NK+ C+ TF+ L L +L L DN + I F +++ L L N CDC LKWL+++ ++N R C SP L + + K F C G++ + FS C +D+ CP KC+CE T +DCS +L IP+ +P TT+L L+ ND+ L + FK L NL+ + LS N I E+ + ++ L+L+ N+L+ +H F+ LS+LK L+L+SN +S TI AF L +L+ I L NPF+C+C++ WL ++ R + +G C P +K I +F C E +
Sbjct: 29 PPAAACPTKCTCSA-ASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSIIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGVTGVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTIGQ-FTLCMAPVHLRGFSVADVQKKEYVCPGPHSEAPACNANS----LSCPSACSCSNNIVDCRGKGLTEIPANLPEGIVEIRLEQNSIKSIPA-GAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIPKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFVPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGAR---CSSPRRLANKRISQIKSKKFRCSGSEDYRNR--------FSSECFMDL------VCPEKCRCEGTIVDCSNQKLARIPSHLPEYTTDLRLNDNDISVLEATGIFKKLPNLRKINLSNNRIKEVREGAFDGAAGVQELMLTGNQLETMHGRMFRGLSSLKTLMLRSNLISCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHMAWLGRWLRKRRIVSGNPRCQKPFFLKEIPIQDVAIQDFTCDGNEESS 718 HSP 4 Score: 286.189 bits (731), Expect = 3.023e-76 Identity = 211/714 (29.55%), Postives = 321/714 (44.96%), Query Frame = -2
Query: 6936 PDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXLTQNGMVDIEPGAFIGLQQLTALYLNMNNLRT------------------------IRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINL------------------------SSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESS-FKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVS------------------------TIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDT 8858
P CP +CTCS V+C G L +P+ +P N LDL N+IT + + + K L+ + L N + IE GAF L+QL L LN N L+ I R AF G++ + L L NN I C+ G F ++ L +L L+ N +S + + K+R L L N LYCDC + WL L+ + C+ P G +V +Q + C + + + CP CSCS+ IV+C G GL+ +P + + L N IK++ + Q L ++D+S NQI +I PD F L + L ++NK+ C+ TF+ L L +L L DN + I F +++ L L N CDC LKWL+++ ++N R C SP L + + K F C G++ + FS C +D+ CP KC+CE T +DCS +L IP+ +P TT+L L+ ND+ L + FK L NL+ + LS N I E+ + ++ L+L+ N+L+ +H F+ LS+LK L+L+SN +S TI AF L +L+ I L NPF+C+C++ WL ++ R + +G C P +K I +F C E +
Sbjct: 29 PPAAACPTKCTCSA-ASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSIIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGVTGVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTIGQ-FTLCMAPVHLRGFSVADVQKKEYVCPGPHSEAPACNANS----LSCPSACSCSNNIVDCRGKGLTEIPANLPEGIVEIRLEQNSIKSIPA-GAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIPKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFVPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGAR---CSSPRRLANKRISQIKSKKFRCSGSEDYRNR--------FSSECFMDL------VCPEKCRCEGTIVDCSNQKLARIPSHLPEYTTDLRLNDNDISVLEATGIFKKLPNLRKINLSNNRIKEVREGAFDGAAGVQELMLTGNQLETMHGRMFRGLSSLKTLMLRSNLISCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHMAWLGRWLRKRRIVSGNPRCQKPFFLKEIPIQDVAIQDFTCDGNEESS 718 HSP 5 Score: 208.379 bits (529), Expect = 3.175e-52 Identity = 138/440 (31.36%), Postives = 219/440 (49.77%), Query Frame = 2
Query: 119 ICPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFF-KLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXXQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISE-AITKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQC-ANEENTCQPDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXXTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDI 1429
+CP+ C+CE V+C ++ +P+ L E TT L L N I V+ + F KL L+ + LSNNRI + GAFD + +L L N ++ + +F LS L+ L LR N + C++ F+ L ++ L L N++ I+ A T L L + + SN +CNCH+ WL +L+ K I + R C P L +IPI ++ +F C NEE++CQ + CP+QCTC + +V C+ + L +P+ +P + EL L+ N +T + P +QLT + L+ N++ + + F+ +S L L L N + C+P F G++ L +L L N +S++ E L L +L L NPL+CDC + WL + +K +A C P S + + + FQC DI
Sbjct: 504 VCPEKCRCEGTIVDCSNQKLARIPSHLPEYTTDLRLNDNDISVLEATGIFKKLPNLRKINLSNNRIKEVREGAFDGAAGVQELMLTGNQLETMHGRMFRGLSSLKTLMLRSNLISCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHMAWLGRWLR-KRRIVSGNPR---CQKPFFLKEIPIQDVAIQDFTCDGNEESSCQLSPR-CPEQCTCVET-VVRCSNRGLHALPKGMPKDVTELYLEGNHLTAV-------------------------PKELSAFRQLTLIDLSNNSISMLTNHTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVK-AGYKEPGIARCSSPESMADRLLLTTPTHRFQCKGPVDI 911 HSP 6 Score: 208.379 bits (529), Expect = 3.175e-52 Identity = 138/440 (31.36%), Postives = 219/440 (49.77%), Query Frame = -2
Query: 8202 ICPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFF-KLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXIQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISE-AITKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQC-ANEENTCQPDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXLTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDI 9512
+CP+ C+CE V+C ++ +P+ L E TT L L N I V+ + F KL L+ + LSNNRI + GAFD + +L L N ++ + +F LS L+ L LR N + C++ F+ L ++ L L N++ I+ A T L L + + SN +CNCH+ WL +L+ K I + R C P L +IPI ++ +F C NEE++CQ + CP+QCTC + +V C+ + L +P+ +P + EL L+ N +T + P +QLT + L+ N++ + + F+ +S L L L N + C+P F G++ L +L L N +S++ E L L +L L NPL+CDC + WL + +K +A C P S + + + FQC DI
Sbjct: 504 VCPEKCRCEGTIVDCSNQKLARIPSHLPEYTTDLRLNDNDISVLEATGIFKKLPNLRKINLSNNRIKEVREGAFDGAAGVQELMLTGNQLETMHGRMFRGLSSLKTLMLRSNLISCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHMAWLGRWLR-KRRIVSGNPR---CQKPFFLKEIPIQDVAIQDFTCDGNEESSCQLSPR-CPEQCTCVET-VVRCSNRGLHALPKGMPKDVTELYLEGNHLTAV-------------------------PKELSAFRQLTLIDLSNNSISMLTNHTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVK-AGYKEPGIARCSSPESMADRLLLTTPTHRFQCKGPVDI 911
BLAST of slit vs. uniprot
Match: gi|311033528|sp|O75094|SLIT3_HUMAN (RecName: Full=Slit homolog 3 protein; Short=Slit-3; AltName: Full=Multiple epidermal growth factor-like domains protein 5; Short=Multiple EGF-like domains protein 5; Flags: Precursor) HSP 1 Score: 723.005 bits (1865), Expect = 0.000e+0 Identity = 488/1580 (30.89%), Postives = 768/1580 (48.61%), Query Frame = 2
Query: 122 CPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXXQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISEA----------------------ITKLKHL---RELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCA---NEENTCQPDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXXTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLK------------------------CVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGN-YECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYC-HDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNSLYF-GKDSHIEIHPPSRGFLMPKGNISLKFITNSSSGLILYQGFDKSYLVVNLFRGHIKISYNIGDKDIGPDG--FSMDVVNDNKLHYLNIEIVGENLTMYLNGKVHFTLKSKYLMTNQYMALDTGIFIGGAPKKILNTAIQNHHFDNDSDFTGCIESLLINRKKIDFSKYMSKSVNVKPGCGDLI-------------SKTAQELPSSEVPTQDIEVP-------------IKSTKSLPVKSTICSKPGVCSNNG---------VCH----EVNEKNRKYKCVCKNGFVGRKCKKRVSKCHGKTTRTFIKDPDTHDNCLSIKKFQYRVCKGTCSNNRMQADSNRNFYQRSTDNSLSPCCQPSKYTTHKIQFKCDNGRFYRRSVTLPRSCKC 4573
CP C C SV+C R VP + RL+L N I I K DF L L+VL L +N++S IE GAF +L L +LRLN+N +Q LP+ LF KL +LDL ENQ+Q + F + +K L L+ N + I + +T H+ R LR+ SN L+C+CHL WL+ +L+ + + + +C+ P L ++ + E+ C +E +C + CP CTCS + IV+C GK L IP +LP +E+ L+ NSI + G ++YK LK+I +++N + DI P AF GL+ LT+L L N + I + F+GL SL L L+ N+I CL TF+ ++ L LL L +NKL + + + PL ++ L+L NP CDC + WL L+ + T A C P + +++ F+C+ D + E C ++CP KC C IV+CS L +P + T L L+ N++ L+ + NL K++LSNN+I+ ++ F + ++E+ L+ N+L+ CV+N+TF L + +L L DN I+ I +F +L + L +N +C+C L WL +W R + G+ P CQ P LK P+ ++ + F C+GN EE+S C P+CP +C C T + CS L+ +P +P + TELYL+ N L + L +L + LS N I+ + + + +L TL+LS+N+L+CI AF L +L+VL L ND+S++P +F DLT+L+++ALG NP HCDC+++WL+++ + + GI+ C+SPE M + + + F C D I AKC+AC+ +PC+NNG C+ Y C CP + GK C IN C PC++GG C +D+ + + C CP GF G +C N DDC + CEN ATCVD +N Y C CP + G+ C+ +C + N C++ C PL + + C C G+ GK CE + DDC + C++ A+C D +NGY C C G+ G +CE P P L + S C +EC N C+ + +Q+ C+C PG++GP C+ L ++ F GKDS++E+ + + P+ NISL+ T+ +G++LY+G D L + L++GH+++ Y D P +S++ VND + H + + + + L + ++ +L L + +++ +++GG P +A++ F GCI + IN + DF +S+ V PGC S + P P D E + + S K +C N CH ++++ Y C+C+ GF G C++ + C G+ R I+ + +C + K C+G C CCQP++ K F+C +G + V C C
Sbjct: 34 CPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTV----GQFTLCMAPVHLRGFNVADVQKKEYVCPAPHSEPPSCNANSISCPSPCTCSNN-IVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIVKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIE-TSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRSRFSSE-CFMDLVCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIVSGN---PRCQKPFFLKEIPIQDVAIQDFTCDGN----EESS----------------CQLSPRCPEQCTCMETVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAV-PRELSALRHLTLIDLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPEPMADRLLLTTPTHRFQCKGPV-DINIVAKCNACLSSPCKNNGTCTQDPVELYRCACPYSYKGKDCTVPINTCIQNPCQHGGTCHLSDSHKDGFSCSCPLGFEGQRCEINPDDCEDNDCENNATCVDGINNYVCICPPNYTGELCDEVIDHCVPELNLCQHEAKCIPL-DKGFSCECVPGYSGKLCETDNDDCVAHKCRHGAQCVDTINGYTCTCPQGFSGPFCEHPP--PMVL------------------------LQTSPCDQYECQNGAQCI---VVQQEPTCRCPPGFAGPRCEKLITVNFVGKDSYVEL---ASAKVRPQANISLQVATDKDNGILLYKG-DNDPLALELYQGHVRLVY---DSLSSPPTTVYSVETVNDGQFHSVELVTLNQTLNLVVDKGTPKSLGK--LQKQPAVGINSPLYLGGIPTSTGLSALRQGTDRPLGGFHGCIHEVRINNELQDFKALPPQSLGVSPGCKSCTVCKHGLCRSVEKDSVVCECRPGWTGPLCDQEARDPCLGHRCHHGKCVATGTSYMCKCAEGYGGDLCDNKNDSANACSAFKCHHGQCHISDQGEPY-CLCQPGFSGEHCQQE-NPCLGQVVREVIRRQKGYASCATASKVPIMECRGGCGPQ---------------------CCQPTRSKRRKYVFQCTDGSSFVEEVERHLECGC 1519 HSP 2 Score: 723.005 bits (1865), Expect = 0.000e+0 Identity = 488/1580 (30.89%), Postives = 768/1580 (48.61%), Query Frame = -2
Query: 5058 CPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXIQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISEA----------------------ITKLKHL---RELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCA---NEENTCQPDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXLTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLK------------------------CVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGN-YECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYC-HDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNSLYF-GKDSHIEIHPPSRGFLMPKGNISLKFITNSSSGLILYQGFDKSYLVVNLFRGHIKISYNIGDKDIGPDG--FSMDVVNDNKLHYLNIEIVGENLTMYLNGKVHFTLKSKYLMTNQYMALDTGIFIGGAPKKILNTAIQNHHFDNDSDFTGCIESLLINRKKIDFSKYMSKSVNVKPGCGDLI-------------SKTAQELPSSEVPTQDIEVP-------------IKSTKSLPVKSTICSKPGVCSNNG---------VCH----EVNEKNRKYKCVCKNGFVGRKCKKRVSKCHGKTTRTFIKDPDTHDNCLSIKKFQYRVCKGTCSNNRMQADSNRNFYQRSTDNSLSPCCQPSKYTTHKIQFKCDNGRFYRRSVTLPRSCKC 9509
CP C C SV+C R VP + RL+L N I I K DF L L+VL L +N++S IE GAF +L L +LRLN+N +Q LP+ LF KL +LDL ENQ+Q + F + +K L L+ N + I + +T H+ R LR+ SN L+C+CHL WL+ +L+ + + + +C+ P L ++ + E+ C +E +C + CP CTCS + IV+C GK L IP +LP +E+ L+ NSI + G ++YK LK+I +++N + DI P AF GL+ LT+L L N + I + F+GL SL L L+ N+I CL TF+ ++ L LL L +NKL + + + PL ++ L+L NP CDC + WL L+ + T A C P + +++ F+C+ D + E C ++CP KC C IV+CS L +P + T L L+ N++ L+ + NL K++LSNN+I+ ++ F + ++E+ L+ N+L+ CV+N+TF L + +L L DN I+ I +F +L + L +N +C+C L WL +W R + G+ P CQ P LK P+ ++ + F C+GN EE+S C P+CP +C C T + CS L+ +P +P + TELYL+ N L + L +L + LS N I+ + + + +L TL+LS+N+L+CI AF L +L+VL L ND+S++P +F DLT+L+++ALG NP HCDC+++WL+++ + + GI+ C+SPE M + + + F C D I AKC+AC+ +PC+NNG C+ Y C CP + GK C IN C PC++GG C +D+ + + C CP GF G +C N DDC + CEN ATCVD +N Y C CP + G+ C+ +C + N C++ C PL + + C C G+ GK CE + DDC + C++ A+C D +NGY C C G+ G +CE P P L + S C +EC N C+ + +Q+ C+C PG++GP C+ L ++ F GKDS++E+ + + P+ NISL+ T+ +G++LY+G D L + L++GH+++ Y D P +S++ VND + H + + + + L + ++ +L L + +++ +++GG P +A++ F GCI + IN + DF +S+ V PGC S + P P D E + + S K +C N CH ++++ Y C+C+ GF G C++ + C G+ R I+ + +C + K C+G C CCQP++ K F+C +G + V C C
Sbjct: 34 CPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTV----GQFTLCMAPVHLRGFNVADVQKKEYVCPAPHSEPPSCNANSISCPSPCTCSNN-IVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIVKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIE-TSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRSRFSSE-CFMDLVCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIVSGN---PRCQKPFFLKEIPIQDVAIQDFTCDGN----EESS----------------CQLSPRCPEQCTCMETVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAV-PRELSALRHLTLIDLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPEPMADRLLLTTPTHRFQCKGPV-DINIVAKCNACLSSPCKNNGTCTQDPVELYRCACPYSYKGKDCTVPINTCIQNPCQHGGTCHLSDSHKDGFSCSCPLGFEGQRCEINPDDCEDNDCENNATCVDGINNYVCICPPNYTGELCDEVIDHCVPELNLCQHEAKCIPL-DKGFSCECVPGYSGKLCETDNDDCVAHKCRHGAQCVDTINGYTCTCPQGFSGPFCEHPP--PMVL------------------------LQTSPCDQYECQNGAQCI---VVQQEPTCRCPPGFAGPRCEKLITVNFVGKDSYVEL---ASAKVRPQANISLQVATDKDNGILLYKG-DNDPLALELYQGHVRLVY---DSLSSPPTTVYSVETVNDGQFHSVELVTLNQTLNLVVDKGTPKSLGK--LQKQPAVGINSPLYLGGIPTSTGLSALRQGTDRPLGGFHGCIHEVRINNELQDFKALPPQSLGVSPGCKSCTVCKHGLCRSVEKDSVVCECRPGWTGPLCDQEARDPCLGHRCHHGKCVATGTSYMCKCAEGYGGDLCDNKNDSANACSAFKCHHGQCHISDQGEPY-CLCQPGFSGEHCQQE-NPCLGQVVREVIRRQKGYASCATASKVPIMECRGGCGPQ---------------------CCQPTRSKRRKYVFQCTDGSSFVEEVERHLECGC 1519 HSP 3 Score: 281.952 bits (720), Expect = 6.725e-75 Identity = 216/739 (29.23%), Postives = 331/739 (44.79%), Query Frame = 2
Query: 773 PDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXXTQNGMVDIEPGAFIGLQQLTALYLNMNNLRT------------------------IRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVI-CPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINL------------------------SSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESS-FKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQS------------------------NDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDT-YITAKCD---ACMKT--PCQNNG 2749
P CP +CTCS V+C G L +P+ +P N LDL N+IT + + + K L+ + L N + IE GAF L+QL L LN N L+ I R AF G++ + L L NN I C+ G F ++ L +L L+ N +S + + K+R L L N LYCDC + WL L+ + C+ P G V +Q + C + + +C I CP C+CS+ IV+C G GL +P + + L N IK + + Q L ++D+S NQI +I PD F L + L ++NK+ C+ TF+ L L +L L DN + I F +++ L L N CDC LKWL+++ ++N R C SP L + + K F C G++ ++ FS C +D+ CP KC+CE T +DCS +L IP+ +P T+L L+ N++ L + FK L NL+ + LS N I E+ + +++ L+L+ N+L+ +H F+ LS LK L+L+S N ++TI AF L +L+ I L NPF+C+C++ WL ++ R + +G C P +K I +F C E + ++ +C CM+T C N G
Sbjct: 29 PPAVACPTKCTCSA-ASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTVGQ-FTLCMAPVHLRGFNVADVQKKEYVCPAP-----HSEPPSCNANSISCPSPCTCSNNIVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPA-GAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIVKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGAR---CSSPRRLANKRISQIKSKKFRCSGSED--------YRSRFSSECFMDL------VCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIVSGNPRCQKPFFLKEIPIQDVAIQDFTCDGNEESSCQLSPRCPEQCTCMETVVRCSNKG 742 HSP 4 Score: 281.952 bits (720), Expect = 6.725e-75 Identity = 216/739 (29.23%), Postives = 331/739 (44.79%), Query Frame = -2
Query: 6882 PDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXLTQNGMVDIEPGAFIGLQQLTALYLNMNNLRT------------------------IRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVI-CPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINL------------------------SSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESS-FKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQS------------------------NDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDT-YITAKCD---ACMKT--PCQNNG 8858
P CP +CTCS V+C G L +P+ +P N LDL N+IT + + + K L+ + L N + IE GAF L+QL L LN N L+ I R AF G++ + L L NN I C+ G F ++ L +L L+ N +S + + K+R L L N LYCDC + WL L+ + C+ P G V +Q + C + + +C I CP C+CS+ IV+C G GL +P + + L N IK + + Q L ++D+S NQI +I PD F L + L ++NK+ C+ TF+ L L +L L DN + I F +++ L L N CDC LKWL+++ ++N R C SP L + + K F C G++ ++ FS C +D+ CP KC+CE T +DCS +L IP+ +P T+L L+ N++ L + FK L NL+ + LS N I E+ + +++ L+L+ N+L+ +H F+ LS LK L+L+S N ++TI AF L +L+ I L NPF+C+C++ WL ++ R + +G C P +K I +F C E + ++ +C CM+T C N G
Sbjct: 29 PPAVACPTKCTCSA-ASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTVGQ-FTLCMAPVHLRGFNVADVQKKEYVCPAP-----HSEPPSCNANSISCPSPCTCSNNIVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPA-GAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIVKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGAR---CSSPRRLANKRISQIKSKKFRCSGSED--------YRSRFSSECFMDL------VCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIVSGNPRCQKPFFLKEIPIQDVAIQDFTCDGNEESSCQLSPRCPEQCTCMETVVRCSNKG 742 HSP 5 Score: 201.445 bits (511), Expect = 4.215e-50 Identity = 136/440 (30.91%), Postives = 217/440 (49.32%), Query Frame = 2
Query: 119 ICPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFF-KLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXXQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISE-AITKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQC-ANEENTCQPDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXXTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDI 1429
+CP+ C+CE V+C ++ +P+ L E T L L N + V+ + F KL L+ + LSNN+I + GAFD ++ +L L N ++ + +F LS L+ L LR N + C++ F+ L ++ L L N++ I+ A T L L + + SN +CNCHL WL +L+ K I + R C P L +IPI ++ +F C NEE++CQ + CP+QCTC + +V C+ K L +P+ +P + EL L+ N +T + P L+ LT + L+ N++ + F+ +S L L L N + C+P F G++ L +L L N +S++ E L L +L L NPL+CDC + WL + +K +A C P + + + FQC DI
Sbjct: 504 VCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLR-KRRIVSGNPR---CQKPFFLKEIPIQDVAIQDFTCDGNEESSCQLSPR-CPEQCTC-METVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAV-------------------------PRELSALRHLTLIDLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVK-AGYKEPGIARCSSPEPMADRLLLTTPTHRFQCKGPVDI 911 HSP 6 Score: 201.445 bits (511), Expect = 4.215e-50 Identity = 136/440 (30.91%), Postives = 217/440 (49.32%), Query Frame = -2
Query: 8202 ICPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFF-KLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXIQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISE-AITKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQC-ANEENTCQPDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXLTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDI 9512
+CP+ C+CE V+C ++ +P+ L E T L L N + V+ + F KL L+ + LSNN+I + GAFD ++ +L L N ++ + +F LS L+ L LR N + C++ F+ L ++ L L N++ I+ A T L L + + SN +CNCHL WL +L+ K I + R C P L +IPI ++ +F C NEE++CQ + CP+QCTC + +V C+ K L +P+ +P + EL L+ N +T + P L+ LT + L+ N++ + F+ +S L L L N + C+P F G++ L +L L N +S++ E L L +L L NPL+CDC + WL + +K +A C P + + + FQC DI
Sbjct: 504 VCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLR-KRRIVSGNPR---CQKPFFLKEIPIQDVAIQDFTCDGNEESSCQLSPR-CPEQCTC-METVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAV-------------------------PRELSALRHLTLIDLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVK-AGYKEPGIARCSSPEPMADRLLLTTPTHRFQCKGPVDI 911
BLAST of slit vs. uniprot
Match: gi|45477219|sp|O88279|SLIT1_RAT (RecName: Full=Slit homolog 1 protein; Short=Slit-1; AltName: Full=Multiple epidermal growth factor-like domains protein 4; Short=Multiple EGF-like domains protein 4; Flags: Precursor) HSP 1 Score: 705.286 bits (1819), Expect = 0.000e+0 Identity = 488/1591 (30.67%), Postives = 747/1591 (46.95%), Query Frame = 2
Query: 122 CPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXXQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISEA----------------------ITKLKH---LRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCANEENT-----CQPDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXXTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDI----KYDTH-DENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLK------------------------CVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNF-GNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCH-DKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNSLYF-GKDSHIEIHPPSRGFLMPKGNISLKFITNSSSGLILYQGFDKSYLVVNLFRGHIKISYNIGDKDIGPDGFSMDVVNDNKLHYLNIEIVGENLTMYLNGKVHFTLK--SKYLMTNQYMALDTGIFIGGAPKKILNTAIQNHHFDNDSDFTGCIESLLINRKKIDFSKYMSKSVNVKPGC---------GDLISKTAQELPSSEVPT--------QDIEVPIKSTKSLPVK------------------STICSKPGVCSN--------NGVCHEVNEKNRKYKCVCKNGFVGRKCKKRVSKCHGKTTRTFIKDPDTHDNCLSIKKFQYRVCKGTCSNNRMQADSNRNFYQRSTDNSLSPCCQPSKYTTHKIQFKCDNGRFYRRSVTLPRSCKC 4573
CP C C +V+C + +P + T RL L GN I I K+DF L L+VLQL N+I ++E GAFD++ L +LRLNRN +Q LP+ LF L +LDL EN LQ + F LK L L+ N++ I E ++ H LR R+ SN L C+CHL WL+ +L+ + I C P L + ++ + EF C+ + C CP C+CS +GIV+C GK LT IP +LP E+ L+ N I + G S Y+ L++I L+ N + +I P AF GL+ L +L L N + + R F GL +L L L+ N+I C+ F+ ++ L LL L +NK+ +L + L ++ L+L NP CDC + WL L+ + T A C P + +++ F+C+++ D H + C V CPHKC C + +V CSG+ LS +PE+I STT L L++N+I L+ + S+L K++LSNN++ I+ TF +T + E++L++N+L+ C++N++F L + +L L DNHI+ I +F L L L N +C+C+L WL +W R + G+ P CQ+P L+ PL ++ F CE +EE + C P+CP +C C T + CS L+ +P IP N TELYLD N TL L+ + LS N I+ ++ T + L TL+LS+N LQCI AF+ L +L++L L NDVST+ F D+T+L+++A+G NP +CDC+++WL+ + + + GI+ CA P M+ K + + +F C + + AKCD C+ +PCQN G C Y C CP+ + G++CE +++C PC NGG C + + C CPSGF G C N DDC H C NG CVD + Y+C+CP + G+ CE +C D NPC++ C +C C G+ G NC +N DDCK + C+N A+C D +N Y C+C GY G CE P + C EC N CV++ + C+C PG+ GP C+ L S+ F +D++++ P+ NI+L+ T +G++LY G D ++ V L++GH+++SY+ G +S + +ND + H + + + + + ++G T+ K+ N L ++GG P + + A + N + F GCI +L IN + DF+K K V PGC + A P Q ++ P K + K +C++ G + +G C R CVC GF G C++ S+C G R F + + C + + + C+G C CCQ + K+ F+C +G + V P C C
Sbjct: 34 CPALCTCTGTTVDCHGTGLQAIPKNIPRNTERLELNGNNITRIHKNDFAGLKQLRVLQLMENQIGAVERGAFDDMKELERLRLNRNQLQVLPELLFQNNQALSRLDLSENSLQAVPRKAFRGATDLKNLQLDKNQISCIEEGAFRALRGLEVLTLNNNNITTIPVSSFNHMPKLRTFRLHSNHLFCDCHLAWLSQWLRQRPTI----GLFTQCSGPASLRGLNVAEVQKSEFSCSGQGEAAQVPACTLSSGSCPAMCSCS-NGIVDCRGKGLTAIPANLPETMTEIRLELNGIKSIPPGAFSPYRKLRRIDLSNNQIAEIAPDAFQGLRSLNSLVLYGNKITDLPRGVFGGLYTLQLLLLNANKINCIRPDAFQDLQNLSLLSLYDNKIQSLAKGTFTSLRAIQTLHLAQNPFICDCNLKWLADFLRTNPIETT-GARCASPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYHLNSECTSDVACPHKCRCEASVVECSGLKLSKIPERIPQSTTELRLNNNEISILEATGLFKKLSHLKKINLSNNKVSEIEDGTFEGATSVSELHLTANQLESVRSGMFRGLDGLRTLMLRNNRISCIHNDSFTGLRNVRLLSLYDNHITTISPGAFDTLQALSTLNLLANPFNCNCQLAWLGDWLRKRKIVTGN---PRCQNPDFLRQIPLQDVAFPDFRCE---EGQEE----------------VGCLPRPQCPQECACLDTVVRCSNKHLQALPKGIPKNVTELYLDGNQF-TLVPGQLSTFKYLQLVDLSNNKISSLSNSSFTNMSQLTTLILSYNALQCIPPLAFQGLRSLRLLSLHGNDVSTLQEGIFADVTSLSHLAIGANPLYCDCHLRWLSSWVKTGYKEPGIARCAGPPEMEGKLLLTTPAKKFEC-QGPPSLAVQAKCDPCLSSPCQNQGTCHNDPLEVYRCTCPSGYKGRNCEVSLDSCSSNPCGNGGTCHAQEGEDAGFTCSCPSGFEGLTCGMNTDDCVKHDCVNGGVCVDGIGNYTCQCPLQYTGRACEQLVDFCSPDLNPCQHEAQCVGTPEGP-RCECVPGYTGDNCSKNQDDCKDHQCQNGAQCVDEINSYACLCAEGYSGQLCEIPP------------------------------APRNSCEGTECQNGANCVDQG---SRPVCQCLPGFGGPECEKLLSVNFVDRDTYLQFTDLQN---WPRANITLQVSTAEDNGILLYNG-DNDHIAVELYQGHVRVSYDPGSYPSSAI-YSAETINDGQFHTVELVTFDQMVNLSIDGGSPMTMDNFGKHYTLNSEAPL----YVGGMPVDVNSAAFRLWQILNGTSFHGCIRNLYINNELQDFTKTQMKP-GVVPGCEPCRKLYCLHGICQPNATPGPVCHCEAGWGGLHCDQPVDGPCHGHKCVHGKCVPLDALAYSCQCQDGYSGALCNQVGAVAEPCGGLQCLHGHCQA--SATRGAHCVCSPGFSGELCEQE-SECRGDPVRDFHRVQRGYAICQTTRPLSWVECRGACPGQG--------------------CCQGLRLKRRKLTFECSDGTSFAEEVEKPTKCGC 1527 HSP 2 Score: 705.286 bits (1819), Expect = 0.000e+0 Identity = 488/1591 (30.67%), Postives = 747/1591 (46.95%), Query Frame = -2
Query: 5058 CPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXIQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISEA----------------------ITKLKH---LRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCANEENT-----CQPDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXLTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDI----KYDTH-DENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLK------------------------CVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNF-GNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCH-DKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNSLYF-GKDSHIEIHPPSRGFLMPKGNISLKFITNSSSGLILYQGFDKSYLVVNLFRGHIKISYNIGDKDIGPDGFSMDVVNDNKLHYLNIEIVGENLTMYLNGKVHFTLK--SKYLMTNQYMALDTGIFIGGAPKKILNTAIQNHHFDNDSDFTGCIESLLINRKKIDFSKYMSKSVNVKPGC---------GDLISKTAQELPSSEVPT--------QDIEVPIKSTKSLPVK------------------STICSKPGVCSN--------NGVCHEVNEKNRKYKCVCKNGFVGRKCKKRVSKCHGKTTRTFIKDPDTHDNCLSIKKFQYRVCKGTCSNNRMQADSNRNFYQRSTDNSLSPCCQPSKYTTHKIQFKCDNGRFYRRSVTLPRSCKC 9509
CP C C +V+C + +P + T RL L GN I I K+DF L L+VLQL N+I ++E GAFD++ L +LRLNRN +Q LP+ LF L +LDL EN LQ + F LK L L+ N++ I E ++ H LR R+ SN L C+CHL WL+ +L+ + I C P L + ++ + EF C+ + C CP C+CS +GIV+C GK LT IP +LP E+ L+ N I + G S Y+ L++I L+ N + +I P AF GL+ L +L L N + + R F GL +L L L+ N+I C+ F+ ++ L LL L +NK+ +L + L ++ L+L NP CDC + WL L+ + T A C P + +++ F+C+++ D H + C V CPHKC C + +V CSG+ LS +PE+I STT L L++N+I L+ + S+L K++LSNN++ I+ TF +T + E++L++N+L+ C++N++F L + +L L DNHI+ I +F L L L N +C+C+L WL +W R + G+ P CQ+P L+ PL ++ F CE +EE + C P+CP +C C T + CS L+ +P IP N TELYLD N TL L+ + LS N I+ ++ T + L TL+LS+N LQCI AF+ L +L++L L NDVST+ F D+T+L+++A+G NP +CDC+++WL+ + + + GI+ CA P M+ K + + +F C + + AKCD C+ +PCQN G C Y C CP+ + G++CE +++C PC NGG C + + C CPSGF G C N DDC H C NG CVD + Y+C+CP + G+ CE +C D NPC++ C +C C G+ G NC +N DDCK + C+N A+C D +N Y C+C GY G CE P + C EC N CV++ + C+C PG+ GP C+ L S+ F +D++++ P+ NI+L+ T +G++LY G D ++ V L++GH+++SY+ G +S + +ND + H + + + + + ++G T+ K+ N L ++GG P + + A + N + F GCI +L IN + DF+K K V PGC + A P Q ++ P K + K +C++ G + +G C R CVC GF G C++ S+C G R F + + C + + + C+G C CCQ + K+ F+C +G + V P C C
Sbjct: 34 CPALCTCTGTTVDCHGTGLQAIPKNIPRNTERLELNGNNITRIHKNDFAGLKQLRVLQLMENQIGAVERGAFDDMKELERLRLNRNQLQVLPELLFQNNQALSRLDLSENSLQAVPRKAFRGATDLKNLQLDKNQISCIEEGAFRALRGLEVLTLNNNNITTIPVSSFNHMPKLRTFRLHSNHLFCDCHLAWLSQWLRQRPTI----GLFTQCSGPASLRGLNVAEVQKSEFSCSGQGEAAQVPACTLSSGSCPAMCSCS-NGIVDCRGKGLTAIPANLPETMTEIRLELNGIKSIPPGAFSPYRKLRRIDLSNNQIAEIAPDAFQGLRSLNSLVLYGNKITDLPRGVFGGLYTLQLLLLNANKINCIRPDAFQDLQNLSLLSLYDNKIQSLAKGTFTSLRAIQTLHLAQNPFICDCNLKWLADFLRTNPIETT-GARCASPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYHLNSECTSDVACPHKCRCEASVVECSGLKLSKIPERIPQSTTELRLNNNEISILEATGLFKKLSHLKKINLSNNKVSEIEDGTFEGATSVSELHLTANQLESVRSGMFRGLDGLRTLMLRNNRISCIHNDSFTGLRNVRLLSLYDNHITTISPGAFDTLQALSTLNLLANPFNCNCQLAWLGDWLRKRKIVTGN---PRCQNPDFLRQIPLQDVAFPDFRCE---EGQEE----------------VGCLPRPQCPQECACLDTVVRCSNKHLQALPKGIPKNVTELYLDGNQF-TLVPGQLSTFKYLQLVDLSNNKISSLSNSSFTNMSQLTTLILSYNALQCIPPLAFQGLRSLRLLSLHGNDVSTLQEGIFADVTSLSHLAIGANPLYCDCHLRWLSSWVKTGYKEPGIARCAGPPEMEGKLLLTTPAKKFEC-QGPPSLAVQAKCDPCLSSPCQNQGTCHNDPLEVYRCTCPSGYKGRNCEVSLDSCSSNPCGNGGTCHAQEGEDAGFTCSCPSGFEGLTCGMNTDDCVKHDCVNGGVCVDGIGNYTCQCPLQYTGRACEQLVDFCSPDLNPCQHEAQCVGTPEGP-RCECVPGYTGDNCSKNQDDCKDHQCQNGAQCVDEINSYACLCAEGYSGQLCEIPP------------------------------APRNSCEGTECQNGANCVDQG---SRPVCQCLPGFGGPECEKLLSVNFVDRDTYLQFTDLQN---WPRANITLQVSTAEDNGILLYNG-DNDHIAVELYQGHVRVSYDPGSYPSSAI-YSAETINDGQFHTVELVTFDQMVNLSIDGGSPMTMDNFGKHYTLNSEAPL----YVGGMPVDVNSAAFRLWQILNGTSFHGCIRNLYINNELQDFTKTQMKP-GVVPGCEPCRKLYCLHGICQPNATPGPVCHCEAGWGGLHCDQPVDGPCHGHKCVHGKCVPLDALAYSCQCQDGYSGALCNQVGAVAEPCGGLQCLHGHCQA--SATRGAHCVCSPGFSGELCEQE-SECRGDPVRDFHRVQRGYAICQTTRPLSWVECRGACPGQG--------------------CCQGLRLKRRKLTFECSDGTSFAEEVEKPTKCGC 1527
BLAST of slit vs. Smed Unigenes AA
Match: SMU15036778 (dd_smedV4_12111_0_1|m.4973|m.1592) HSP 1 Score: 2139.77 bits (5543), Expect = 0.000e+0 Identity = 1091/1537 (70.98%), Postives = 1293/1537 (84.12%), Query Frame = 2
Query: 8 MQFIFKDITXXXXXXXXXXXXXXXXTIWINDPSEEVEICPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXXQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISEAITKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCANE-----ENTCQPDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXXTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCL--DMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNK-QKSYCKCKPGYSGPFCQYLNSLYFGKDSHIEIHPPSRGFLMPKGNISLKFITNSSSGLILYQGFDKSYLVVNLFRGHIKISYNIGDKDIGPDGFSMDVVNDNKLHYLNIEIVGENLTMYLNGKVHFTLKSKYLMTNQYMALDTGIFIGGAPKKILNTAIQNHHFDNDSDFTGCIESLLINRKKIDFSKYMSKSVNVKPGCGDLISKTAQ---ELPSSEVPTQDIEVPIKSTKSLPVKSTICSKPGVCSNNGVCHEVNEKNRKYKCVCKNGFVGRKCKKRVSKCHGKTTRTFIKDPDTHDNCLSIKKFQYRVCKGTCSNNRMQADSNRNFYQRSTDNSLSPCCQPSKYTTHKIQFKCDNGRFYRRSVTLPRSCKCQGNC 4585
M FIFKDIT+K L+LL SLYCFSSTIWINDPS+E EICP GCQCEN+SVECKHRLF NVP IL EKTTRLNLLGNRI+VIRKSDF KLN LKVLQLSNNRI+SIE GAFDNL+NLIKLRLNRNLIQ+LPD LFSKL+KLQKLDLR+NQLQC+N+++F+KLGMLK LLLEGNK++WISEAI+KLKHL+ELRI NKL+CNCHL WLN FLK KS+I KP SV+C TP RLS IPIS+LS++EFQCA+E EN C D+KVC K+C CS DGIV C GK+L+NIPQDLPTN +ELDL++N+ITIL+QG+LSKYK LKKIILT+NGMVDIEPGAF+GL QLT++YLN NNL+TIR+NAF GL SLMFLYL N+IECLP T E KRL+LLHLSENKL++LK+E +P + LRYLYLVHNPL CDC++ W+V +K KE N TPVA+C+ P + G +++LQ YNFQCN E + KY+THDE CVPKV CP KC+CSS++VNCS GL SLPE IFPST TLI+SHN++K L IYS+H+A NL+KLDLS NQIE ++PD F++S+KL+EINL+SN+LKC+NNETFK+L ELEILQL++N ISCI T+SFK N NLKYLMLN N+ HCDC+LKW+SEWSRN+Y +LG+ RLPTCQSP ILK TP+ +LD+KYF+C+GN S SC + N +CC + +C K KCPSKCKCEST++DCS+LQLKEIP EIP +TTELYLDRN L++LNE+SFKNL+NLKTLVLSYNGITE+NK+VLTPLK+LETLVLSFNKLQCIHQDAFK+L NLKVLILQSNDVSTIP+QAF DL NLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPE+MKLKSIYHSKPT+FICSEETED YITAKCD+CMK PC++NGKCSLITHH+Y CDCP PFHGK+C+KRINACFGQPCRNGGICKN DN+GNYECVCP GFRGPKCT N+DDC G+ C NGATC D N YSCKC +GFRGK+C+ KFLYC+D NPC+NSG C+ L NNDYKC C+KGWEGK CE+N DDCKYN CK+E++C DL N YKC+CRPGY+G +CE PAWKP LKT +SRRDI++SE++ NEVISESVCAYHECLNNG+C+N D NK QKSYCKCKPGYSG CQYLNS+YF K+S+IEI PPSRGFLMP+GNISL F+TNSSSGLILYQG +++YLVVNL++GH++ISYN+GDK +GP+G+SM +VND++LH L+IEIVGENLTMYLNG+VH TLKSKYL +N+YMALDTGIFIGGAP KIL +AI H + DS+F GC+ESLLINRKKIDFSKY+ KS+NV PGCG LIS ++PS P + I ++TK P + +C +PG+C N GVC + +K++C+CKNGFVGRKCKKR S CHG T + I DPD+ C SI++FQYR+C GTCS +RMQ+D +NFY + D S S CCQP+KY+TH+IQFKC+NGR YRR+ LP++CKC NC
Sbjct: 1 MIFIFKDITFKFLLVLLSSLYCFSSTIWINDPSDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDNLVNLIKLRLNRNLIQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETN-TPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREINLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFKNNLNLKYLMLNKNQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNEECTKSKKCPSKCKCESTKVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLE-NEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMQPIKQILT--QTTKKKP--NNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCVANC 1531 HSP 2 Score: 2139.77 bits (5543), Expect = 0.000e+0 Identity = 1091/1537 (70.98%), Postives = 1293/1537 (84.12%), Query Frame = -2
Query: 5046 MQFIFKDITXXXXXXXXXXXXXXXSTIWINDPSEEVEICPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXIQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISEAITKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCANE-----ENTCQPDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXLTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCL--DMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNK-QKSYCKCKPGYSGPFCQYLNSLYFGKDSHIEIHPPSRGFLMPKGNISLKFITNSSSGLILYQGFDKSYLVVNLFRGHIKISYNIGDKDIGPDGFSMDVVNDNKLHYLNIEIVGENLTMYLNGKVHFTLKSKYLMTNQYMALDTGIFIGGAPKKILNTAIQNHHFDNDSDFTGCIESLLINRKKIDFSKYMSKSVNVKPGCGDLISKTAQ---ELPSSEVPTQDIEVPIKSTKSLPVKSTICSKPGVCSNNGVCHEVNEKNRKYKCVCKNGFVGRKCKKRVSKCHGKTTRTFIKDPDTHDNCLSIKKFQYRVCKGTCSNNRMQADSNRNFYQRSTDNSLSPCCQPSKYTTHKIQFKCDNGRFYRRSVTLPRSCKCQGNC 9623
M FIFKDIT+K L+LL SLYCFSSTIWINDPS+E EICP GCQCEN+SVECKHRLF NVP IL EKTTRLNLLGNRI+VIRKSDF KLN LKVLQLSNNRI+SIE GAFDNL+NLIKLRLNRNLIQ+LPD LFSKL+KLQKLDLR+NQLQC+N+++F+KLGMLK LLLEGNK++WISEAI+KLKHL+ELRI NKL+CNCHL WLN FLK KS+I KP SV+C TP RLS IPIS+LS++EFQCA+E EN C D+KVC K+C CS DGIV C GK+L+NIPQDLPTN +ELDL++N+ITIL+QG+LSKYK LKKIILT+NGMVDIEPGAF+GL QLT++YLN NNL+TIR+NAF GL SLMFLYL N+IECLP T E KRL+LLHLSENKL++LK+E +P + LRYLYLVHNPL CDC++ W+V +K KE N TPVA+C+ P + G +++LQ YNFQCN E + KY+THDE CVPKV CP KC+CSS++VNCS GL SLPE IFPST TLI+SHN++K L IYS+H+A NL+KLDLS NQIE ++PD F++S+KL+EINL+SN+LKC+NNETFK+L ELEILQL++N ISCI T+SFK N NLKYLMLN N+ HCDC+LKW+SEWSRN+Y +LG+ RLPTCQSP ILK TP+ +LD+KYF+C+GN S SC + N +CC + +C K KCPSKCKCEST++DCS+LQLKEIP EIP +TTELYLDRN L++LNE+SFKNL+NLKTLVLSYNGITE+NK+VLTPLK+LETLVLSFNKLQCIHQDAFK+L NLKVLILQSNDVSTIP+QAF DL NLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPE+MKLKSIYHSKPT+FICSEETED YITAKCD+CMK PC++NGKCSLITHH+Y CDCP PFHGK+C+KRINACFGQPCRNGGICKN DN+GNYECVCP GFRGPKCT N+DDC G+ C NGATC D N YSCKC +GFRGK+C+ KFLYC+D NPC+NSG C+ L NNDYKC C+KGWEGK CE+N DDCKYN CK+E++C DL N YKC+CRPGY+G +CE PAWKP LKT +SRRDI++SE++ NEVISESVCAYHECLNNG+C+N D NK QKSYCKCKPGYSG CQYLNS+YF K+S+IEI PPSRGFLMP+GNISL F+TNSSSGLILYQG +++YLVVNL++GH++ISYN+GDK +GP+G+SM +VND++LH L+IEIVGENLTMYLNG+VH TLKSKYL +N+YMALDTGIFIGGAP KIL +AI H + DS+F GC+ESLLINRKKIDFSKY+ KS+NV PGCG LIS ++PS P + I ++TK P + +C +PG+C N GVC + +K++C+CKNGFVGRKCKKR S CHG T + I DPD+ C SI++FQYR+C GTCS +RMQ+D +NFY + D S S CCQP+KY+TH+IQFKC+NGR YRR+ LP++CKC NC
Sbjct: 1 MIFIFKDITFKFLLVLLSSLYCFSSTIWINDPSDESEICPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDNLVNLIKLRLNRNLIQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYLLLEGNKIFWISEAISKLKHLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKANLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETN-TPVAACLQPRNLQGTPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESIFPSTKTLIMSHNNLKKLPPIYSIHEAPNLMKLDLSFNQIETLEPDVFTRSSKLREINLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFKNNLNLKYLMLNKNQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVYHSVDSCHETNSVKTCCSIGNEECTKSKKCPSKCKCESTKVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPLKSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPEKMKLKSIYHSKPTDFICSEETEDAYITAKCDSCMKRPCKHNGKCSLITHHNYQCDCPYPFHGKNCDKRINACFGQPCRNGGICKNIDNYGNYECVCPVGFRGPKCTINVDDCVGNLCVNGATCNDLNNSYSCKCQSGFRGKYCDIKFLYCNDINPCQNSGQCQLLPNNDYKCVCSKGWEGKQCERNTDDCKYNSCKHESQCVDLHNDYKCMCRPGYIGKFCEIPAWKPTVLKTLISRRDINQSEMTYENLE-NEVISESVCAYHECLNNGICINGDQNKNQKSYCKCKPGYSGHLCQYLNSIYFQKNSYIEISPPSRGFLMPRGNISLNFMTNSSSGLILYQGSEQTYLVVNLYQGHLRISYNLGDKVVGPEGYSMGMVNDSRLHALHIEIVGENLTMYLNGRVHCTLKSKYLNSNRYMALDTGIFIGGAPHKILQSAIMKFHIETDSEFIGCMESLLINRKKIDFSKYLPKSINVHPGCGKLISTVVASDLKIPSIMQPIKQILT--QTTKKKP--NNVCKRPGICKNQGVCEVLPGSKKKFRCICKNGFVGRKCKKRKSMCHGTFTESHIVDPDSQGTCKSIERFQYRICTGTCSTSRMQSDVIKNFYHKPIDPSSSRCCQPTKYSTHEIQFKCENGRVYRRAANLPKNCKCVANC 1531
BLAST of slit vs. Smed Unigenes AA
Match: SMU15006317 (SmedAsxldir_c107934_g5_i1|m.86273|m.14433) HSP 1 Score: 143.28 bits (360), Expect = 1.973e-33 Identity = 82/190 (43.16%), Postives = 107/190 (56.32%), Query Frame = 2
Query: 2720 CMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCE 3289
C PC+N G C DY C C N + GK+C+ N C PC+N G C++ N+ +Y+C+C GF G C +ID+C C NG +C++ + C C GF G CE C +K PC+N+ C L NNDY C C +G GKNCE NID+C N C N A+CED V G+ C C G+VG CE
Sbjct: 256 CELKPCKNGGICRDF-DEDYQCICGNQWTGKNCDDVKNPCKINPCKNNGFCESV-NYQDYKCICQRGFEGKWCETSIDNCQKISCFNGGSCINMIEGGKCNCLPGFEGNNCEINVNECENK-PCQNNAKCIDL-NNDYTCDCPEGLTGKNCEINIDECTPNPCLNGARCEDRVGGFTCQCLQGFVGLRCE 441 HSP 2 Score: 143.28 bits (360), Expect = 1.973e-33 Identity = 82/190 (43.16%), Postives = 107/190 (56.32%), Query Frame = -2
Query: 6342 CMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCE 6911
C PC+N G C DY C C N + GK+C+ N C PC+N G C++ N+ +Y+C+C GF G C +ID+C C NG +C++ + C C GF G CE C +K PC+N+ C L NNDY C C +G GKNCE NID+C N C N A+CED V G+ C C G+VG CE
Sbjct: 256 CELKPCKNGGICRDF-DEDYQCICGNQWTGKNCDDVKNPCKINPCKNNGFCESV-NYQDYKCICQRGFEGKWCETSIDNCQKISCFNGGSCINMIEGGKCNCLPGFEGNNCEINVNECENK-PCQNNAKCIDL-NNDYTCDCPEGLTGKNCEINIDECTPNPCLNGARCEDRVGGFTCQCLQGFVGLRCE 441 HSP 3 Score: 135.961 bits (341), Expect = 3.349e-31 Identity = 101/307 (32.90%), Postives = 142/307 (46.25%), Query Frame = 2
Query: 2726 KTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINAC--FGQP-CRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDK-------------------------------------NPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCE----EPAWKPYKLKTNLSRRDIDRS-EVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQ 3511
+ PC NGKC L + C C + G +C I+ C + P C NGG C NT G+Y+C C +GF G +C +I DC C+NG TC+D++ Y C CPN + G CE+K C K NPC+N+G CE ++ DYKC C +G+EGK CE +ID+C+ C N C +++ G KC C PG+ G CE E KP + + D + + + T N I+ C + CLN C ED + C+C G+ G C+
Sbjct: 140 QNPCGVNGKCILANDGEVKCHCNKGYTGNTCSNDIDECASYETPLCENGGTCINT--VGSYQCKCKAGFNGERCENDIVDCTNDTCKNGGTCLDKIGSYRCVCPNNYFGTNCENKRTLCELKPCKNGGICRDFDEDYQCICGNQWTGKNCDDVKNPCKINPCKNNGFCESVNYQDYKCICQRGFEGKWCETSIDNCQKISCFNGGSCINMIEGGKCNCLPGFEGNNCEINVNECENKPCQNNAKCIDLNNDYTCDCPEGLTGKNCEINIDECTPNPCLNGARC--EDRVGGFT-CQCLQGFVGLRCE 441 HSP 4 Score: 135.961 bits (341), Expect = 3.349e-31 Identity = 101/307 (32.90%), Postives = 142/307 (46.25%), Query Frame = -2
Query: 6120 KTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINAC--FGQP-CRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDK-------------------------------------NPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCE----EPAWKPYKLKTNLSRRDIDRS-EVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQ 6905
+ PC NGKC L + C C + G +C I+ C + P C NGG C NT G+Y+C C +GF G +C +I DC C+NG TC+D++ Y C CPN + G CE+K C K NPC+N+G CE ++ DYKC C +G+EGK CE +ID+C+ C N C +++ G KC C PG+ G CE E KP + + D + + + T N I+ C + CLN C ED + C+C G+ G C+
Sbjct: 140 QNPCGVNGKCILANDGEVKCHCNKGYTGNTCSNDIDECASYETPLCENGGTCINT--VGSYQCKCKAGFNGERCENDIVDCTNDTCKNGGTCLDKIGSYRCVCPNNYFGTNCENKRTLCELKPCKNGGICRDFDEDYQCICGNQWTGKNCDDVKNPCKINPCKNNGFCESVNYQDYKCICQRGFEGKWCETSIDNCQKISCFNGGSCINMIEGGKCNCLPGFEGNNCEINVNECENKPCQNNAKCIDLNNDYTCDCPEGLTGKNCEINIDECTPNPCLNGARC--EDRVGGFT-CQCLQGFVGLRCE 441 HSP 5 Score: 127.102 bits (318), Expect = 1.665e-28 Identity = 98/318 (30.82%), Postives = 136/318 (42.77%), Query Frame = 2
Query: 2669 ICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDKNPCEN--------------------------------------SGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFC 3508
IC E + D C K C N G C + C+C F G +CE +N C +PC+N C + +N +Y C CP G G C NID+C + C NGA C D+V ++C+C GF G CE+K C PC+ +G C + NN+ KC C G+EG+ CE I++C N C++ ++C+DL N Y CIC PG+ G CE +INE CA++ C NG C++E + C+C YSGP C
Sbjct: 316 ICQRGFEGKWCETSIDNCQKISCFNGGSCINMIEGGK-CNCLPGFEGNNCEINVNECENKPCQNNAKCIDLNN--DYTCDCPEGLTGKNCEINIDECTPNPCLNGARCEDRVGGFTCQCLQGFVGLRCETKSDICKQLTPCKTEEICLNVNNTRSGYKCICPDGVQNCKKELSKCKNIVCINGQCVDV-NNEEKCICIPGFEGRTCEIEINECFNNPCQHGSQCQDLFNDYLCICLPGFKGKKCE---------------------------ININE------CAHNPCGLNGQCIDEVYGFR---CQCMLPYSGPTC 593 HSP 6 Score: 127.102 bits (318), Expect = 1.665e-28 Identity = 98/318 (30.82%), Postives = 136/318 (42.77%), Query Frame = -2
Query: 6123 ICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDKNPCEN--------------------------------------SGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFC 6962
IC E + D C K C N G C + C+C F G +CE +N C +PC+N C + +N +Y C CP G G C NID+C + C NGA C D+V ++C+C GF G CE+K C PC+ +G C + NN+ KC C G+EG+ CE I++C N C++ ++C+DL N Y CIC PG+ G CE +INE CA++ C NG C++E + C+C YSGP C
Sbjct: 316 ICQRGFEGKWCETSIDNCQKISCFNGGSCINMIEGGK-CNCLPGFEGNNCEINVNECENKPCQNNAKCIDLNN--DYTCDCPEGLTGKNCEINIDECTPNPCLNGARCEDRVGGFTCQCLQGFVGLRCETKSDICKQLTPCKTEEICLNVNNTRSGYKCICPDGVQNCKKELSKCKNIVCINGQCVDV-NNEEKCICIPGFEGRTCEIEINECFNNPCQHGSQCQDLFNDYLCICLPGFKGKKCE---------------------------ININE------CAHNPCGLNGQCIDEVYGFR---CQCMLPYSGPTC 593 HSP 7 Score: 108.612 bits (270), Expect = 6.092e-23 Identity = 86/264 (32.58%), Postives = 119/264 (45.08%), Query Frame = 2
Query: 2732 PCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDKNPCENSGHCEPLSNND----YKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQ 3511
PC+N C + + C C F G+ CE IN C PC N C D+ +Y+C+C SGF G C N +DC C CVD+VN + CKC + G CE C D NPC N G C N Y C+C +G+ G CE +D C+ N C + C+ ++ +KC C Y+G CE + + + +S + F NE + +C +H G C N D C C+ GY G FC+
Sbjct: 705 PCRNGATCDNLA-DGFRCRCVAGFTGERCEIEINECQHNPCSNDAKC--IDHINDYQCICQSGFSGKNCEINDNDCTESSCLGHGQCVDKVNGFECKCNENYIGTNCEHLKRPC-DSNPCLNKGSCINRKVNQNTPGYLCNCRRGFTGPRCEIKMDWCRGNPCGDFGVCKTDLDSFKCKCNSEYIGERCE------------IKKTETCKSYAVEKFK--NESLINKICKHH-----GNCFN-DPETGGFKCSCQSGYGGSFCE 944 HSP 8 Score: 108.612 bits (270), Expect = 6.092e-23 Identity = 86/264 (32.58%), Postives = 119/264 (45.08%), Query Frame = -2
Query: 6120 PCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDKNPCENSGHCEPLSNND----YKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQ 6899
PC+N C + + C C F G+ CE IN C PC N C D+ +Y+C+C SGF G C N +DC C CVD+VN + CKC + G CE C D NPC N G C N Y C+C +G+ G CE +D C+ N C + C+ ++ +KC C Y+G CE + + + +S + F NE + +C +H G C N D C C+ GY G FC+
Sbjct: 705 PCRNGATCDNLA-DGFRCRCVAGFTGERCEIEINECQHNPCSNDAKC--IDHINDYQCICQSGFSGKNCEINDNDCTESSCLGHGQCVDKVNGFECKCNENYIGTNCEHLKRPC-DSNPCLNKGSCINRKVNQNTPGYLCNCRRGFTGPRCEIKMDWCRGNPCGDFGVCKTDLDSFKCKCNSEYIGERCE------------IKKTETCKSYAVEKFK--NESLINKICKHH-----GNCFN-DPETGGFKCSCQSGYGGSFCE 944 HSP 9 Score: 107.457 bits (267), Expect = 1.447e-22 Identity = 83/308 (26.95%), Postives = 139/308 (45.13%), Query Frame = 2
Query: 2714 DACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSG---FRGPKCTENIDDCGGHK--------------------CENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVIS-----------------ESVCAYHECLNNGVCVNEDMNKQKS--YCKCKPGYSGPFCQ 3511
+ C PC NG+C + + + C C P+ G +C +++ C C N IC + + +Y C C + + G KC +++D+C + C N C++ + CKC + + G+ CE K L NPC N C+ L++ ++C C G+ G+ CE I++C++N C N+AKC D +N Y+CIC+ G+ G CE ++ D S H +++V + C + CLN G C+N +N+ C C+ G++GP C+
Sbjct: 560 NECAHNPCGLNGQC-IDEVYGFRCQCMLPYSGPTCANKLDPCNPNACYNDAICIPGNKYDSYHCNCSTALGRYEGSKCEKDVDECNLDRTQLALYGLAVLSMSNSQIGPCLNDNQCINTNGSFICKCSSQYEGRQCE-KPLNNCRNNPCRNGATCDNLADG-FRCRCVAGFTGERCEIEINECQHNPCSNDAKCIDHINDYQCICQSGFSGKNCE------------INDNDCTESSCLGHGQCVDKVNGFECKCNENYIGTNCEHLKRPCDSNPCLNKGSCINRKVNQNTPGYLCNCRRGFTGPRCE 852 HSP 10 Score: 107.457 bits (267), Expect = 1.447e-22 Identity = 83/308 (26.95%), Postives = 139/308 (45.13%), Query Frame = -2
Query: 6120 DACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSG---FRGPKCTENIDDCGGHK--------------------CENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVIS-----------------ESVCAYHECLNNGVCVNEDMNKQKS--YCKCKPGYSGPFCQ 6917
+ C PC NG+C + + + C C P+ G +C +++ C C N IC + + +Y C C + + G KC +++D+C + C N C++ + CKC + + G+ CE K L NPC N C+ L++ ++C C G+ G+ CE I++C++N C N+AKC D +N Y+CIC+ G+ G CE ++ D S H +++V + C + CLN G C+N +N+ C C+ G++GP C+
Sbjct: 560 NECAHNPCGLNGQC-IDEVYGFRCQCMLPYSGPTCANKLDPCNPNACYNDAICIPGNKYDSYHCNCSTALGRYEGSKCEKDVDECNLDRTQLALYGLAVLSMSNSQIGPCLNDNQCINTNGSFICKCSSQYEGRQCE-KPLNNCRNNPCRNGATCDNLADG-FRCRCVAGFTGERCEIEINECQHNPCSNDAKCIDHINDYQCICQSGFSGKNCE------------INDNDCTESSCLGHGQCVDKVNGFECKCNENYIGTNCEHLKRPCDSNPCLNKGSCINRKVNQNTPGYLCNCRRGFTGPRCE 852 HSP 11 Score: 107.071 bits (266), Expect = 1.831e-22 Identity = 95/324 (29.32%), Postives = 130/324 (40.12%), Query Frame = 2
Query: 2672 CSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYC-------------HDKNPCENSGHCEPLSN-NDYKCSCTKGWEGKNCEQNIDDCKY------------NVCKNEAKCEDLVN-GYKCICRPGYVGTYCEEPAWK-------------PYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNS 3523
C ++ D C PC N G+C T Y C C F G +C+ N C+ C G K D N+ CVCP G G C +D C +C+NG C + + + C CP GF GK CE+ C + PC +G E L + CSC K + GK CE ID C+ +C N+ + E N + CIC GY G C+ + K P+++ +S + S V F I+ V + C +C NNG CVN K+ C C G G C+ S
Sbjct: 934 CQSGYGGSFCEVNIDDCASQPCINGGECGDGTGM-YFCKCQPGFQGVNCQVNTNECYSNSCNTG---KCIDQINNFSCVCPRGVSGRFCETIVDYCKKMECQNGGICKNVIGDFECNCPKGFAGKLCETDINECKYNIAGNATNLISNPHGPCSPTGTLECLDRPGTFHCSCKKYFTGKYCETRIDYCEKFRKSGNIPCFNNGICLNKYQNEVGRNYNFSCICPNGYGGERCKINSKKCQLNVCKNNGICRPFEIDDRMSFKCECPSGVGGLFCEIDYV---NECLSTKCYNNGSCVN---GFGKAICHCNKGSCGEKCEITGS 1247 HSP 12 Score: 107.071 bits (266), Expect = 1.831e-22 Identity = 95/324 (29.32%), Postives = 130/324 (40.12%), Query Frame = -2
Query: 6108 CSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYC-------------HDKNPCENSGHCEPLSN-NDYKCSCTKGWEGKNCEQNIDDCKY------------NVCKNEAKCEDLVN-GYKCICRPGYVGTYCEEPAWK-------------PYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNS 6959
C ++ D C PC N G+C T Y C C F G +C+ N C+ C G K D N+ CVCP G G C +D C +C+NG C + + + C CP GF GK CE+ C + PC +G E L + CSC K + GK CE ID C+ +C N+ + E N + CIC GY G C+ + K P+++ +S + S V F I+ V + C +C NNG CVN K+ C C G G C+ S
Sbjct: 934 CQSGYGGSFCEVNIDDCASQPCINGGECGDGTGM-YFCKCQPGFQGVNCQVNTNECYSNSCNTG---KCIDQINNFSCVCPRGVSGRFCETIVDYCKKMECQNGGICKNVIGDFECNCPKGFAGKLCETDINECKYNIAGNATNLISNPHGPCSPTGTLECLDRPGTFHCSCKKYFTGKYCETRIDYCEKFRKSGNIPCFNNGICLNKYQNEVGRNYNFSCICPNGYGGERCKINSKKCQLNVCKNNGICRPFEIDDRMSFKCECPSGVGGLFCEIDYV---NECLSTKCYNNGSCVN---GFGKAICHCNKGSCGEKCEITGS 1247 HSP 13 Score: 105.916 bits (263), Expect = 3.645e-22 Identity = 92/318 (28.93%), Postives = 130/318 (40.88%), Query Frame = 2
Query: 2699 ITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVD-QVNKYSCKCPNGFRGKFCESKFLYCHDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNID-----------------------------------------DCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEP----AWKPYKLKTNLSRRDIDRSEV-SKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQ 3511
I + C C+N C + Y+C+C + G CE++ N C PC+NGG CK GNY C C + + G C E +C C NG C +++KY C+CP + G CE C +NPC +G C ++ + KC C KG+ G C +ID DC + CKN C D + Y+C+C Y GT CE KP K D D + +T N ++ C + C NNG C E +N Q C C+ G+ G +C+
Sbjct: 17 IAISLNFCENHKCKNGATCQSDINGKYVCECAYGYKGTYCEQKSN-CPITPCQNGGTCKLI--AGNYVCQCLNEWVGEFC-EFSSNCASSPCRNGGICKSLEIHKYECQCPKNYLGMNCELPN-SCGSQNPCGVNGKCILANDGEVKCHCNKGYTGNTCSNDIDECASYETPLCENGGTCINTVGSYQCKCKAGFNGERCENDIVDCTNDTCKNGGTCLDKIGSYRCVCPNNYFGTNCENKRTLCELKPCKNGGICRDFDEDYQCICGNQWTGKNCDDVKNPCKINPCKNNGFC--ESVNYQDYKCICQRGFEGKWCE 327 HSP 14 Score: 105.916 bits (263), Expect = 3.645e-22 Identity = 92/318 (28.93%), Postives = 130/318 (40.88%), Query Frame = -2
Query: 6120 ITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVD-QVNKYSCKCPNGFRGKFCESKFLYCHDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNID-----------------------------------------DCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEP----AWKPYKLKTNLSRRDIDRSEV-SKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQ 6932
I + C C+N C + Y+C+C + G CE++ N C PC+NGG CK GNY C C + + G C E +C C NG C +++KY C+CP + G CE C +NPC +G C ++ + KC C KG+ G C +ID DC + CKN C D + Y+C+C Y GT CE KP K D D + +T N ++ C + C NNG C E +N Q C C+ G+ G +C+
Sbjct: 17 IAISLNFCENHKCKNGATCQSDINGKYVCECAYGYKGTYCEQKSN-CPITPCQNGGTCKLI--AGNYVCQCLNEWVGEFC-EFSSNCASSPCRNGGICKSLEIHKYECQCPKNYLGMNCELPN-SCGSQNPCGVNGKCILANDGEVKCHCNKGYTGNTCSNDIDECASYETPLCENGGTCINTVGSYQCKCKAGFNGERCENDIVDCTNDTCKNGGTCLDKIGSYRCVCPNNYFGTNCENKRTLCELKPCKNGGICRDFDEDYQCICGNQWTGKNCDDVKNPCKINPCKNNGFC--ESVNYQDYKCICQRGFEGKWCE 327 HSP 15 Score: 99.7525 bits (247), Expect = 2.532e-20 Identity = 123/452 (27.21%), Postives = 175/452 (38.72%), Query Frame = 2
Query: 2324 EINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQ-----NPFHCDCNIKWL--NQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFI-CSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCE-KRINAC-------------FGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEP--------AWKPYKLKTN-------------LSRRDIDRSEVSKHFTSINEVISESVCAYHE---------CLNNGVCVNEDMNK-QKSY---CKCKPGYSGPFCQ 3511
EIN+ P N + N QCI Q F + + ND D T + + GQ N F C CN ++ N L R D+ + C + + + + P C K D C PC + G C + C C + + G+ CE K+ C + C++ G C N G ++C C SG+ G C NIDDC C NG C D Y CKC GF+G C+ C+ N C N+G C NN + C C +G G+ CE +D CK C+N C++++ ++C C G+ G CE A L +N L R K+FT E+ Y E C NNG+C+N+ N+ ++Y C C GY G C+
Sbjct: 735 EINECQHNPCSNDAKCIDHINDYQCICQSGFSGKN------CEIND---------NDCTESSCLGHGQCVDKVNGFECKCNENYIGTNCEHLKRPCDS--NPCLNKGSCINRKVNQNTPGYLCNCRRGFTGPRCEIKMDWCRGNPCGDFGVCK-TDLDSFKCKCNSEYIGERCEIKKTETCKSYAVEKFKNESLINKICKHHGNCFNDPETGGFKCSCQSGYGGSFCEVNIDDCASQPCINGGECGDGTGMYFCKCQPGFQGVNCQVNTNECY-SNSC-NTGKCIDQINN-FSCVCPRGVSGRFCETIVDYCKKMECQNGGICKNVIGDFECNCPKGFAGKLCETDINECKYNIAGNATNLISNPHGPCSPTGTLECLDRPGTFHCSCKKYFTG---KYCETRIDYCEKFRKSGNIPCFNNGICLNKYQNEVGRNYNFSCICPNGYGGERCK 1162 HSP 16 Score: 99.7525 bits (247), Expect = 2.532e-20 Identity = 123/452 (27.21%), Postives = 175/452 (38.72%), Query Frame = -2
Query: 6120 EINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQ-----NPFHCDCNIKWL--NQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFI-CSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCE-KRINAC-------------FGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEP--------AWKPYKLKTN-------------LSRRDIDRSEVSKHFTSINEVISESVCAYHE---------CLNNGVCVNEDMNK-QKSY---CKCKPGYSGPFCQ 7307
EIN+ P N + N QCI Q F + + ND D T + + GQ N F C CN ++ N L R D+ + C + + + + P C K D C PC + G C + C C + + G+ CE K+ C + C++ G C N G ++C C SG+ G C NIDDC C NG C D Y CKC GF+G C+ C+ N C N+G C NN + C C +G G+ CE +D CK C+N C++++ ++C C G+ G CE A L +N L R K+FT E+ Y E C NNG+C+N+ N+ ++Y C C GY G C+
Sbjct: 735 EINECQHNPCSNDAKCIDHINDYQCICQSGFSGKN------CEIND---------NDCTESSCLGHGQCVDKVNGFECKCNENYIGTNCEHLKRPCDS--NPCLNKGSCINRKVNQNTPGYLCNCRRGFTGPRCEIKMDWCRGNPCGDFGVCK-TDLDSFKCKCNSEYIGERCEIKKTETCKSYAVEKFKNESLINKICKHHGNCFNDPETGGFKCSCQSGYGGSFCEVNIDDCASQPCINGGECGDGTGMYFCKCQPGFQGVNCQVNTNECY-SNSC-NTGKCIDQINN-FSCVCPRGVSGRFCETIVDYCKKMECQNGGICKNVIGDFECNCPKGFAGKLCETDINECKYNIAGNATNLISNPHGPCSPTGTLECLDRPGTFHCSCKKYFTG---KYCETRIDYCEKFRKSGNIPCFNNGICLNKYQNEVGRNYNFSCICPNGYGGERCK 1162
BLAST of slit vs. Smed Unigenes AA
Match: SMU15006140 (SmedAsxldir_c107649_g2_i1|m.84323|m.17798) HSP 1 Score: 129.798 bits (325), Expect = 1.777e-29 Identity = 104/336 (30.95%), Postives = 144/336 (42.86%), Query Frame = 2
Query: 2669 ICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDK----------------------NPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDI---DRSEVSKHFT---------SINEVISESVCAYHECLNNGVCVN-EDMNKQKSYCKCKPGYSGPFCQYLNSLYFGKDSHIEIHPPSR 3571
+C Y + + C+ PC+NNG C+ + +DY C+C F GK+CE I AC QPC+NGG+C+ D Y C C +G+ G C C + C NGA C + C C F GK CE YC ++ N C G C + C C G+EG +CE +IDDCK N CKN C D +N ++CIC G+ G CEE Y +K+ + E+ F + + S+C + CLN G C +N + C C G+ G CQ+L HI+ P R
Sbjct: 453 LCQPGYTGKYCDKEINECVPNPCKNNGVCT-DSINDYKCNCSTSFKGKNCELNI-ACQNQPCKNGGVCQEVDT--GYTCKCKTGYTGTNCEIYKTGCSPNPCLNGAYCYTINQDFYCHCNKEFWGKRCEHSRPYCGNESCESAYDACSMPQMLNSSGIKHNYCGKYGTCLSHEKEKFICRCMPGFEGDSCEIDIDDCKTNPCKNGGICIDSINNFECICDNGFFGKTCEERT--DYCIKSPCMNYGVCLRHSDELYNDFICECKNNWGGRLCHLPKHSICDSNPCLNGGSCSRLSTLNGFQ--CHCNQGWIGTVCQFL---------HIDFQPCRR 771 HSP 2 Score: 129.798 bits (325), Expect = 1.777e-29 Identity = 104/336 (30.95%), Postives = 144/336 (42.86%), Query Frame = -2
Query: 6060 ICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDK----------------------NPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDI---DRSEVSKHFT---------SINEVISESVCAYHECLNNGVCVN-EDMNKQKSYCKCKPGYSGPFCQYLNSLYFGKDSHIEIHPPSR 6962
+C Y + + C+ PC+NNG C+ + +DY C+C F GK+CE I AC QPC+NGG+C+ D Y C C +G+ G C C + C NGA C + C C F GK CE YC ++ N C G C + C C G+EG +CE +IDDCK N CKN C D +N ++CIC G+ G CEE Y +K+ + E+ F + + S+C + CLN G C +N + C C G+ G CQ+L HI+ P R
Sbjct: 453 LCQPGYTGKYCDKEINECVPNPCKNNGVCT-DSINDYKCNCSTSFKGKNCELNI-ACQNQPCKNGGVCQEVDT--GYTCKCKTGYTGTNCEIYKTGCSPNPCLNGAYCYTINQDFYCHCNKEFWGKRCEHSRPYCGNESCESAYDACSMPQMLNSSGIKHNYCGKYGTCLSHEKEKFICRCMPGFEGDSCEIDIDDCKTNPCKNGGICIDSINNFECICDNGFFGKTCEERT--DYCIKSPCMNYGVCLRHSDELYNDFICECKNNWGGRLCHLPKHSICDSNPCLNGGSCSRLSTLNGFQ--CHCNQGWIGTVCQFL---------HIDFQPCRR 771 HSP 3 Score: 113.62 bits (283), Expect = 1.462e-24 Identity = 81/280 (28.93%), Postives = 134/280 (47.86%), Query Frame = 2
Query: 2534 FHCDCNIKWLNQ-------FFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKC-------------DACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGIC-KNTDN-FGNYECVCPSGFRGPKC-TENIDDCGGHKCENGATC--VDQVNKYSCKCPNGFRGKFCES---KFLYCHDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCE 3289
F+C CN ++ + + + ++ C+ P+ + I H+ ++ E +C D C PC+N G C + + +++ C C N F GK+CE+R + C PC N G+C +++D + ++ C C + + G C C + C NG +C + +N + C C G+ G C+ F C KN C+N G C + ND+ C C G+EGK+C+ N ++C N C N KC D +N Y+CIC G+ G+ C+
Sbjct: 563 FYCHCNKEFWGKRCEHSRPYCGNESCESAYDACSMPQMLNSSGIKHNYCGKYGTCLSHEKEKFICRCMPGFEGDSCEIDIDDCKTNPCKNGGIC-IDSINNFECICDNGFFGKTCEERTDYCIKSPCMNYGVCLRHSDELYNDFICECKNNWGGRLCHLPKHSICDSNPCLNGGSCSRLSTLNGFQCHCNQGWIGTVCQFLHIDFQPCRRKNLCKNEGVCINVGYNDFTCHCKPGFEGKHCDINTNECNPNPCFNGGKCIDKLNSYRCICAKGFYGSNCQ 841 HSP 4 Score: 113.62 bits (283), Expect = 1.462e-24 Identity = 81/280 (28.93%), Postives = 134/280 (47.86%), Query Frame = -2
Query: 6342 FHCDCNIKWLNQ-------FFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKC-------------DACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGIC-KNTDN-FGNYECVCPSGFRGPKC-TENIDDCGGHKCENGATC--VDQVNKYSCKCPNGFRGKFCES---KFLYCHDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCE 7097
F+C CN ++ + + + ++ C+ P+ + I H+ ++ E +C D C PC+N G C + + +++ C C N F GK+CE+R + C PC N G+C +++D + ++ C C + + G C C + C NG +C + +N + C C G+ G C+ F C KN C+N G C + ND+ C C G+EGK+C+ N ++C N C N KC D +N Y+CIC G+ G+ C+
Sbjct: 563 FYCHCNKEFWGKRCEHSRPYCGNESCESAYDACSMPQMLNSSGIKHNYCGKYGTCLSHEKEKFICRCMPGFEGDSCEIDIDDCKTNPCKNGGIC-IDSINNFECICDNGFFGKTCEERTDYCIKSPCMNYGVCLRHSDELYNDFICECKNNWGGRLCHLPKHSICDSNPCLNGGSCSRLSTLNGFQCHCNQGWIGTVCQFLHIDFQPCRRKNLCKNEGVCINVGYNDFTCHCKPGFEGKHCDINTNECNPNPCFNGGKCIDKLNSYRCICAKGFYGSNCQ 841 HSP 5 Score: 108.612 bits (270), Expect = 4.361e-23 Identity = 100/351 (28.49%), Postives = 144/351 (41.03%), Query Frame = 2
Query: 2525 QNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMK-----TPCQNNGKCS--LITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATC-VDQVNKYSCKCPNGFRGKFCESKFLYCHDKNPCENSG-------HCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCE-EPAWKPYKLKTNLSRRDIDRSEVSK---HFTSINEVISESVCAYHECLNNGVC--VNEDMNKQKSYCKCKPGYSGPFCQY 3514
+NP C C W F E MK H + + E+ + CD + PC+N G C L Y C CP+ F G+ CE I+ CF PC+ G K+ G + C C + G C ++ C + C+NG C D+ C C G++G CH PCENS + + DYKC C G+ GK C++ I++C N CKN C D +N YKC C + G CE A + K +++D K +T N I ++ C+ + CLN C +N+D YC C + G C++
Sbjct: 259 ENPGECRCRAGWQGTF--------------CDECMKYPGCKHGTCVDSPWTCSCENNWGGQFCDQSLNYCAENNPCKNGGICENLLSEVQPYKCICPSGFEGRDCE--IDPCFPNPCKKGTCMKSK---GEFYCQCIERWTGKLCDQDTSICDQNSCQNGGQCGKDENGNRRCICQQGYKGS-------ECHLAAPCENSQCKHALDCQIQSTALGDYKCLCQPGYTGKYCDKEINECVPNPCKNNGVCTDSINDYKCNCSTSFKGKNCELNIACQNQPCKNGGVCQEVDTGYTCKCKTGYTGTNCEIYKTGCSPNPCLNGAYCYTINQDF-----YCHCNKEFWGKRCEH 578 HSP 6 Score: 108.612 bits (270), Expect = 4.361e-23 Identity = 100/351 (28.49%), Postives = 144/351 (41.03%), Query Frame = -2
Query: 6117 QNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMK-----TPCQNNGKCS--LITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATC-VDQVNKYSCKCPNGFRGKFCESKFLYCHDKNPCENSG-------HCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCE-EPAWKPYKLKTNLSRRDIDRSEVSK---HFTSINEVISESVCAYHECLNNGVC--VNEDMNKQKSYCKCKPGYSGPFCQY 7106
+NP C C W F E MK H + + E+ + CD + PC+N G C L Y C CP+ F G+ CE I+ CF PC+ G K+ G + C C + G C ++ C + C+NG C D+ C C G++G CH PCENS + + DYKC C G+ GK C++ I++C N CKN C D +N YKC C + G CE A + K +++D K +T N I ++ C+ + CLN C +N+D YC C + G C++
Sbjct: 259 ENPGECRCRAGWQGTF--------------CDECMKYPGCKHGTCVDSPWTCSCENNWGGQFCDQSLNYCAENNPCKNGGICENLLSEVQPYKCICPSGFEGRDCE--IDPCFPNPCKKGTCMKSK---GEFYCQCIERWTGKLCDQDTSICDQNSCQNGGQCGKDENGNRRCICQQGYKGS-------ECHLAAPCENSQCKHALDCQIQSTALGDYKCLCQPGYTGKYCDKEINECVPNPCKNNGVCTDSINDYKCNCSTSFKGKNCELNIACQNQPCKNGGVCQEVDTGYTCKCKTGYTGTNCEIYKTGCSPNPCLNGAYCYTINQDF-----YCHCNKEFWGKRCEH 578 HSP 7 Score: 107.842 bits (268), Expect = 8.010e-23 Identity = 96/372 (25.81%), Postives = 154/372 (41.40%), Query Frame = 2
Query: 2528 NPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHS-----KPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDN---------------------------------------FGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQ 3511
+P+ C C W QF D ++ CA K I + +P + IC E + D C PC+ G C + + ++ C C + GK C++ + C C+NGG C +N G+Y+C+C G+ G C + I++C + C+N C D +N Y C C F+GK CE + C ++ PC+N G C+ + + Y C C G+ G NCE C N C N A C + + C C + G CE +PY + E + S+ ++++ S ++ C G C++ + K+K C+C PG+ G C+
Sbjct: 292 SPWTCSCENNWGGQF-----CDQSLNYCAENNPCKNGGICENLLSEVQPYKCICPSGFEGR--DCEIDPCFPNPCKK-GTC-MKSKGEFYCQCIERWTGKLCDQDTSICDQNSCQNGGQCGKDENGNRRCICQQGYKGSECHLAAPCENSQCKHALDCQIQSTALGDYKCLCQPGYTGKYCDKEINECVPNPCKNNGVCTDSINDYKCNCSTSFKGKNCELN-IACQNQ-PCKNGGVCQEV-DTGYTCKCKTGYTGTNCEIYKTGCSPNPCLNGAYCYTINQDFYCHCNKEFWGKRCEHS--RPYCGN--------ESCESAYDACSMPQMLNSSGIKHNYCGKYGTCLSHE--KEKFICRCMPGFEGDSCE 639 HSP 8 Score: 107.842 bits (268), Expect = 8.010e-23 Identity = 96/372 (25.81%), Postives = 154/372 (41.40%), Query Frame = -2
Query: 6120 NPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHS-----KPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDN---------------------------------------FGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQ 7103
+P+ C C W QF D ++ CA K I + +P + IC E + D C PC+ G C + + ++ C C + GK C++ + C C+NGG C +N G+Y+C+C G+ G C + I++C + C+N C D +N Y C C F+GK CE + C ++ PC+N G C+ + + Y C C G+ G NCE C N C N A C + + C C + G CE +PY + E + S+ ++++ S ++ C G C++ + K+K C+C PG+ G C+
Sbjct: 292 SPWTCSCENNWGGQF-----CDQSLNYCAENNPCKNGGICENLLSEVQPYKCICPSGFEGR--DCEIDPCFPNPCKK-GTC-MKSKGEFYCQCIERWTGKLCDQDTSICDQNSCQNGGQCGKDENGNRRCICQQGYKGSECHLAAPCENSQCKHALDCQIQSTALGDYKCLCQPGYTGKYCDKEINECVPNPCKNNGVCTDSINDYKCNCSTSFKGKNCELN-IACQNQ-PCKNGGVCQEV-DTGYTCKCKTGYTGTNCEIYKTGCSPNPCLNGAYCYTINQDFYCHCNKEFWGKRCEHS--RPYCGN--------ESCESAYDACSMPQMLNSSGIKHNYCGKYGTCLSHE--KEKFICRCMPGFEGDSCE 639 HSP 9 Score: 104.375 bits (259), Expect = 9.586e-22 Identity = 93/294 (31.63%), Postives = 121/294 (41.16%), Query Frame = 2
Query: 2717 ACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNG-------------GICKN-TDNFGN--------YECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDKNPCENSGHCEPLSN---NDYKCSCTKGWEGKNCE-QNIDDCKYNVCKNEAKCEDL--VNGYKCICRPGYVGTYCE--EPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFC 3508
C PC N C I + D+ C C F GK CE C + C + GI N +G + C C GF G C +IDDC + C+NG C+D +N + C C NGF GK CE + YC K+PC N G C S+ ND+ C C W G+ C C N C N C L +NG++C C G++GT C+ ++P + K NL C N GVC+N N C CKPG+ G C
Sbjct: 544 GCSPNPCLNGAYCYTI-NQDFYCHCNKEFWGKRCEHSRPYCGNESCESAYDACSMPQMLNSSGIKHNYCGKYGTCLSHEKEKFICRCMPGFEGDSCEIDIDDCKTNPCKNGGICIDSINNFECICDNGFFGKTCEERTDYC-IKSPCMNYGVCLRHSDELYNDFICECKNNWGGRLCHLPKHSICDSNPCLNGGSCSRLSTLNGFQCHCNQGWIGTVCQFLHIDFQPCRRK-NL------------------------------CKNEGVCINVGYNDFT--CHCKPGFEGKHC 802 HSP 10 Score: 104.375 bits (259), Expect = 9.586e-22 Identity = 93/294 (31.63%), Postives = 121/294 (41.16%), Query Frame = -2
Query: 6123 ACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNG-------------GICKN-TDNFGN--------YECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDKNPCENSGHCEPLSN---NDYKCSCTKGWEGKNCE-QNIDDCKYNVCKNEAKCEDL--VNGYKCICRPGYVGTYCE--EPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFC 6914
C PC N C I + D+ C C F GK CE C + C + GI N +G + C C GF G C +IDDC + C+NG C+D +N + C C NGF GK CE + YC K+PC N G C S+ ND+ C C W G+ C C N C N C L +NG++C C G++GT C+ ++P + K NL C N GVC+N N C CKPG+ G C
Sbjct: 544 GCSPNPCLNGAYCYTI-NQDFYCHCNKEFWGKRCEHSRPYCGNESCESAYDACSMPQMLNSSGIKHNYCGKYGTCLSHEKEKFICRCMPGFEGDSCEIDIDDCKTNPCKNGGICIDSINNFECICDNGFFGKTCEERTDYC-IKSPCMNYGVCLRHSDELYNDFICECKNNWGGRLCHLPKHSICDSNPCLNGGSCSRLSTLNGFQCHCNQGWIGTVCQFLHIDFQPCRRK-NL------------------------------CKNEGVCINVGYNDFT--CHCKPGFEGKHC 802 HSP 11 Score: 95.1301 bits (235), Expect = 5.658e-19 Identity = 73/220 (33.18%), Postives = 106/220 (48.18%), Query Frame = 2
Query: 2663 EFICSEETEDTYITAKCDACMKTPCQNNGKC---SLITHHDYICDCPNPFHGKSCE-KRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCT-ENIDDCGGHK---CENGATCVD-QVNKYSCKCPNGFRGKFCESKFLYCHDKNPCENSGHC-EPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEE 3292
E IC + D C+K+PC N G C S ++D+IC+C N + G+ C + + C PC NGG C ++C C G+ G C +ID + C+N C++ N ++C C GF GK C+ C + NPC N G C + L N Y+C C KG+ G NC+ N D+C C A C D ++ ++C C G G C+
Sbjct: 664 ECICDNGFFGKTCEERTDYCIKSPCMNYGVCLRHSDELYNDFICECKNNWGGRLCHLPKHSICDSNPCLNGGSCSRLSTLNGFQCHCNQGWIGTVCQFLHIDFQPCRRKNLCKNEGVCINVGYNDFTCHCKPGFEGKHCDINTNEC-NPNPCFNGGKCIDKL--NSYRCICAKGFYGSNCQLNYDECASFPCAYGAICVDRIDDFECRCPEGRDGKLCDH 880 HSP 12 Score: 95.1301 bits (235), Expect = 5.658e-19 Identity = 73/220 (33.18%), Postives = 106/220 (48.18%), Query Frame = -2
Query: 6339 EFICSEETEDTYITAKCDACMKTPCQNNGKC---SLITHHDYICDCPNPFHGKSCE-KRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCT-ENIDDCGGHK---CENGATCVD-QVNKYSCKCPNGFRGKFCESKFLYCHDKNPCENSGHC-EPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEE 6968
E IC + D C+K+PC N G C S ++D+IC+C N + G+ C + + C PC NGG C ++C C G+ G C +ID + C+N C++ N ++C C GF GK C+ C + NPC N G C + L N Y+C C KG+ G NC+ N D+C C A C D ++ ++C C G G C+
Sbjct: 664 ECICDNGFFGKTCEERTDYCIKSPCMNYGVCLRHSDELYNDFICECKNNWGGRLCHLPKHSICDSNPCLNGGSCSRLSTLNGFQCHCNQGWIGTVCQFLHIDFQPCRRKNLCKNEGVCINVGYNDFTCHCKPGFEGKHCDINTNEC-NPNPCFNGGKCIDKL--NSYRCICAKGFYGSNCQLNYDECASFPCAYGAICVDRIDDFECRCPEGRDGKLCDH 880
BLAST of slit vs. Smed Unigenes AA
Match: SMU15037114 (dd_smedV4_13823_0_1|m.8312|m.39635) HSP 1 Score: 127.487 bits (319), Expect = 7.392e-29 Identity = 101/369 (27.37%), Postives = 150/369 (40.65%), Query Frame = 2
Query: 2531 PFHCDCNIKWLNQF--FLDRFLDNGISLCASP-ERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSL-ITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNV-CKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSY--------------CKCKPGYSGPFCQYLNSLYFGKDSHIEIHPPSRGFL 3580
P C+C KW F + N ++C + E + +I ++ IC + + C+ C N GKC + + + YICDCP F+G C+ +C PC C TD ++C CP GF G C + D+C C NG C+D+ + + C CP GF G C+ C K C N C +N C CT GW G+ C QNI++C N C N C D Y+C C+ + G +C P +IN + E C + C N+G+C+ + + CKC PG++G C+ + + E + GFL
Sbjct: 325 PLTCNCKKKWGGMFCNIDLNYCSNNPNVCENEGECLNFGNIKTNQQYRCICKPGFKGAHCEKVEHNCIINKCFNGGKCKINVNTNKYICDCPESFYGDRCQFNQTSCDDNPCVGNSECI-TDKTSGFKCKCPPGFIGRNCEIDEDECSSKPCLNGGMCIDRPSGFLCVCPVGFSGDRCQVNKNDCQ-KLSCMNGAKCMDRANR-IDCECTTGWTGRMCNQNINECAINQPCLNNGICIDTPGSYRCQCQATWSGQFCNLKIVVP----------------------NINYL--ERKCLINPCRNSGICILNSLPTSVNIPNNEYFKPIGGPYTCKCNPGFTGDLCEKVIIANSSIVMYSEPQITTEGFL 666 HSP 2 Score: 127.487 bits (319), Expect = 7.392e-29 Identity = 101/369 (27.37%), Postives = 150/369 (40.65%), Query Frame = -2
Query: 6051 PFHCDCNIKWLNQF--FLDRFLDNGISLCASP-ERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSL-ITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNV-CKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSY--------------CKCKPGYSGPFCQYLNSLYFGKDSHIEIHPPSRGFL 7100
P C+C KW F + N ++C + E + +I ++ IC + + C+ C N GKC + + + YICDCP F+G C+ +C PC C TD ++C CP GF G C + D+C C NG C+D+ + + C CP GF G C+ C K C N C +N C CT GW G+ C QNI++C N C N C D Y+C C+ + G +C P +IN + E C + C N+G+C+ + + CKC PG++G C+ + + E + GFL
Sbjct: 325 PLTCNCKKKWGGMFCNIDLNYCSNNPNVCENEGECLNFGNIKTNQQYRCICKPGFKGAHCEKVEHNCIINKCFNGGKCKINVNTNKYICDCPESFYGDRCQFNQTSCDDNPCVGNSECI-TDKTSGFKCKCPPGFIGRNCEIDEDECSSKPCLNGGMCIDRPSGFLCVCPVGFSGDRCQVNKNDCQ-KLSCMNGAKCMDRANR-IDCECTTGWTGRMCNQNINECAINQPCLNNGICIDTPGSYRCQCQATWSGQFCNLKIVVP----------------------NINYL--ERKCLINPCRNSGICILNSLPTSVNIPNNEYFKPIGGPYTCKCNPGFTGDLCEKVIIANSSIVMYSEPQITTEGFL 666 HSP 3 Score: 68.1662 bits (165), Expect = 8.316e-11 Identity = 80/291 (27.49%), Postives = 118/291 (40.55%), Query Frame = 2
Query: 2696 YITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNK----YSCKCPNGFRGKFCESKFLYC-HDKNPCENSGHCEPL----SNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNG--YKCICRPGYVGTYCE--------EPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQ 3511
Y +CD K G + ++ D IC + G C+K + C + C NG G +C C +G+ G C + I G C +G D+ K +C C + G FC YC ++ N CEN G C +N Y+C C G++G +CE+ +C N C N KC+ VN Y C C + G C+ P + T+ + + + F N I E C+ CLN G+C++ C C G+SG CQ
Sbjct: 231 YHGEECDKLCKPSANERGFYNCSSNGDRICH--TGYSGSYCDKVV--CLKK-CINGAC------VGPNQCRCQTGWTGDSCDQCIPYPG---CVHGGCRFDERTKELLPLTCNCKKKWGGMFCNIDLNYCSNNPNVCENEGECLNFGNIKTNQQYRCICKPGFKGAHCEKVEHNCIINKCFNGGKCKINVNTNKYICDCPESFYGDRCQFNQTSCDDNPCVGNSECITD--KTSGFKCKCPPGFIGRNCEIDEDECSSKPCLNGGMCIDR---PSGFLCVCPVGFSGDRCQ 502 HSP 4 Score: 68.1662 bits (165), Expect = 8.316e-11 Identity = 80/291 (27.49%), Postives = 118/291 (40.55%), Query Frame = -2
Query: 6120 YITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNK----YSCKCPNGFRGKFCESKFLYC-HDKNPCENSGHCEPL----SNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNG--YKCICRPGYVGTYCE--------EPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQ 6935
Y +CD K G + ++ D IC + G C+K + C + C NG G +C C +G+ G C + I G C +G D+ K +C C + G FC YC ++ N CEN G C +N Y+C C G++G +CE+ +C N C N KC+ VN Y C C + G C+ P + T+ + + + F N I E C+ CLN G+C++ C C G+SG CQ
Sbjct: 231 YHGEECDKLCKPSANERGFYNCSSNGDRICH--TGYSGSYCDKVV--CLKK-CINGAC------VGPNQCRCQTGWTGDSCDQCIPYPG---CVHGGCRFDERTKELLPLTCNCKKKWGGMFCNIDLNYCSNNPNVCENEGECLNFGNIKTNQQYRCICKPGFKGAHCEKVEHNCIINKCFNGGKCKINVNTNKYICDCPESFYGDRCQFNQTSCDDNPCVGNSECITD--KTSGFKCKCPPGFIGRNCEIDEDECSSKPCLNGGMCIDR---PSGFLCVCPVGFSGDRCQ 502
BLAST of slit vs. Smed Unigenes AA
Match: SMU15036138 (SmedSxlregen_c89796_g1_i1|m.42552|m.12127) HSP 1 Score: 108.612 bits (270), Expect = 1.474e-23 Identity = 91/294 (30.95%), Postives = 124/294 (42.18%), Query Frame = 2
Query: 2696 YITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCV--DQVNKYSCKCPNGFRGKFCESKFLYCHDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLV--------NGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECL------NNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNSLY 3529
++ CD + +PC NN C + + + C C FHGK CE + C PC N G C + G + C+CP + G C NI C KCENG +C D CKC GF G CE+ C +N C G C N+ +C C + G+ CE+ + DC+ N C N C +V C+C G+ G +CE + L +R K++ C C+ N GVCV+ + K CKC P G FCQY N Y
Sbjct: 280 FVKTTCD--LLSPCFNNATCVNLEYDSFKCQCLRGFHGKRCEIP-DHCVDNPCLNDGTCISKK--GRFYCMCPGEYSGRNCELNISKCP--KCENGGSCFTPDMGKNNFCKCLPGFIGHRCETPATKCETENQCLFEGSCFLNIKNEKQCFCPSIFNGRFCEK-LSDCR-NRCLNGGFCSLVVFNTTDLPTAEIVCLCPYGFYGRFCEFAHFCVQSLSPCKNRGACLILNYDKYYCECQTGFHGEKCEMKNCIKDADCQNGGVCVSGERVK----CKCSPSNIGEFCQYKNECY 560 HSP 2 Score: 108.612 bits (270), Expect = 1.474e-23 Identity = 91/294 (30.95%), Postives = 124/294 (42.18%), Query Frame = -2
Query: 6102 YITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCV--DQVNKYSCKCPNGFRGKFCESKFLYCHDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLV--------NGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECL------NNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNSLY 6935
++ CD + +PC NN C + + + C C FHGK CE + C PC N G C + G + C+CP + G C NI C KCENG +C D CKC GF G CE+ C +N C G C N+ +C C + G+ CE+ + DC+ N C N C +V C+C G+ G +CE + L +R K++ C C+ N GVCV+ + K CKC P G FCQY N Y
Sbjct: 280 FVKTTCD--LLSPCFNNATCVNLEYDSFKCQCLRGFHGKRCEIP-DHCVDNPCLNDGTCISKK--GRFYCMCPGEYSGRNCELNISKCP--KCENGGSCFTPDMGKNNFCKCLPGFIGHRCETPATKCETENQCLFEGSCFLNIKNEKQCFCPSIFNGRFCEK-LSDCR-NRCLNGGFCSLVVFNTTDLPTAEIVCLCPYGFYGRFCEFAHFCVQSLSPCKNRGACLILNYDKYYCECQTGFHGEKCEMKNCIKDADCQNGGVCVSGERVK----CKCSPSNIGEFCQYKNECY 560 HSP 3 Score: 72.4034 bits (176), Expect = 3.417e-12 Identity = 70/211 (33.18%), Postives = 98/211 (46.45%), Query Frame = 2
Query: 2702 TAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICK----NTDNFGNYE--CVCPSGFRGPKC------TENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNV-CKNEAKCEDLVNG--YKCICRPGYVGTYCE 3289
KC+ + C G C L ++ C CP+ F+G+ CEK ++ C + C NGG C NT + E C+CP GF G C +++ C GA + +KY C+C GF G+ CE K C C+N G C +S KC C+ G+ C Q ++C NV C KCE V G KC C G++G C+
Sbjct: 397 ATKCET--ENQCLFEGSCFLNIKNEKQCFCPSIFNGRFCEK-LSDCRNR-CLNGGFCSLVVFNTTDLPTAEIVCLCPYGFYGRFCEFAHFCVQSLSPCKNR----GACLILNYDKYYCECQTGFHGEKCEMK--NCIKDADCQNGGVC--VSGERVKCKCSPSNIGEFC-QYKNECYKNVQCGTNGKCETDVEGKFLKCKCNEGWLGKACD 594 HSP 4 Score: 72.4034 bits (176), Expect = 3.417e-12 Identity = 70/211 (33.18%), Postives = 98/211 (46.45%), Query Frame = -2
Query: 6342 TAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICK----NTDNFGNYE--CVCPSGFRGPKC------TENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNV-CKNEAKCEDLVNG--YKCICRPGYVGTYCE 6929
KC+ + C G C L ++ C CP+ F+G+ CEK ++ C + C NGG C NT + E C+CP GF G C +++ C GA + +KY C+C GF G+ CE K C C+N G C +S KC C+ G+ C Q ++C NV C KCE V G KC C G++G C+
Sbjct: 397 ATKCET--ENQCLFEGSCFLNIKNEKQCFCPSIFNGRFCEK-LSDCRNR-CLNGGFCSLVVFNTTDLPTAEIVCLCPYGFYGRFCEFAHFCVQSLSPCKNR----GACLILNYDKYYCECQTGFHGEKCEMK--NCIKDADCQNGGVC--VSGERVKCKCSPSNIGEFC-QYKNECYKNVQCGTNGKCETDVEGKFLKCKCNEGWLGKACD 594 HSP 5 Score: 69.707 bits (169), Expect = 2.403e-11 Identity = 55/163 (33.74%), Postives = 76/163 (46.63%), Query Frame = 2
Query: 2726 KTPCQNNGKCSLITHH-------DYICDCPNPFHGKSCEKRINACFGQ--PCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDKNPCENSGHCEPLSNNDY-KCSCTKGWEGKNCEQNI 3184
+ C N G CSL+ + + +C CP F+G+ CE + C PC+N G C N+ Y C C +GF G KC E + C+NG CV + CKC G+FC+ K C+ C +G CE + KC C +GW GK C+ I
Sbjct: 440 RNRCLNGGFCSLVVFNTTDLPTAEIVCLCPYGFYGRFCE-FAHFCVQSLSPCKNRGACL-ILNYDKYYCECQTGFHGEKC-EMKNCIKDADCQNGGVCVSG-ERVKCKCSPSNIGEFCQYK-NECYKNVQCGTNGKCETDVEGKFLKCKCNEGWLGKACDIQI 597 HSP 6 Score: 69.707 bits (169), Expect = 2.403e-11 Identity = 55/163 (33.74%), Postives = 76/163 (46.63%), Query Frame = -2
Query: 6447 KTPCQNNGKCSLITHH-------DYICDCPNPFHGKSCEKRINACFGQ--PCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDKNPCENSGHCEPLSNNDY-KCSCTKGWEGKNCEQNI 6905
+ C N G CSL+ + + +C CP F+G+ CE + C PC+N G C N+ Y C C +GF G KC E + C+NG CV + CKC G+FC+ K C+ C +G CE + KC C +GW GK C+ I
Sbjct: 440 RNRCLNGGFCSLVVFNTTDLPTAEIVCLCPYGFYGRFCE-FAHFCVQSLSPCKNRGACL-ILNYDKYYCECQTGFHGEKC-EMKNCIKDADCQNGGVCVSG-ERVKCKCSPSNIGEFCQYK-NECYKNVQCGTNGKCETDVEGKFLKCKCNEGWLGKACDIQI 597 HSP 7 Score: 61.2326 bits (147), Expect = 9.330e-9 Identity = 50/182 (27.47%), Postives = 74/182 (40.66%), Query Frame = 2
Query: 2966 HKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQ 3511
++C + C NK +C C + + G +C+ C +PC N+ C L + +KC C +G+ GK CE D C N C N+ C + C+C Y G CE N+S+ +C N G C DM K ++CKC PG+ G C+
Sbjct: 250 YQCSDRGFCEKDGNKINCLCVSPYYGPYCQFVKTTCDLLSPCFNNATCVNLEYDSFKCQCLRGFHGKRCEIP-DHCVDNPCLNDGTCISKKGRFYCMCPGEYSGRNCE----------LNISK-------------------------CPKCENGGSCFTPDMGKN-NFCKCLPGFIGHRCE 394 HSP 8 Score: 61.2326 bits (147), Expect = 9.330e-9 Identity = 50/182 (27.47%), Postives = 74/182 (40.66%), Query Frame = -2
Query: 6120 HKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQ 6665
++C + C NK +C C + + G +C+ C +PC N+ C L + +KC C +G+ GK CE D C N C N+ C + C+C Y G CE N+S+ +C N G C DM K ++CKC PG+ G C+
Sbjct: 250 YQCSDRGFCEKDGNKINCLCVSPYYGPYCQFVKTTCDLLSPCFNNATCVNLEYDSFKCQCLRGFHGKRCEIP-DHCVDNPCLNDGTCISKKGRFYCMCPGEYSGRNCE----------LNISK-------------------------CPKCENGGSCFTPDMGKN-NFCKCLPGFIGHRCE 394
BLAST of slit vs. RefSeq Human
Match: gi|11321571|ref|NP_003053.1| (slit homolog 3 protein isoform 2 precursor [Homo sapiens]) HSP 1 Score: 723.391 bits (1866), Expect = 0.000e+0 Identity = 488/1580 (30.89%), Postives = 768/1580 (48.61%), Query Frame = 2
Query: 122 CPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXXQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISEA----------------------ITKLKHL---RELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCA---NEENTCQPDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXXTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLK------------------------CVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGN-YECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYC-HDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNSLYF-GKDSHIEIHPPSRGFLMPKGNISLKFITNSSSGLILYQGFDKSYLVVNLFRGHIKISYNIGDKDIGPDG--FSMDVVNDNKLHYLNIEIVGENLTMYLNGKVHFTLKSKYLMTNQYMALDTGIFIGGAPKKILNTAIQNHHFDNDSDFTGCIESLLINRKKIDFSKYMSKSVNVKPGCGDLI-------------SKTAQELPSSEVPTQDIEVP-------------IKSTKSLPVKSTICSKPGVCSNNG---------VCH----EVNEKNRKYKCVCKNGFVGRKCKKRVSKCHGKTTRTFIKDPDTHDNCLSIKKFQYRVCKGTCSNNRMQADSNRNFYQRSTDNSLSPCCQPSKYTTHKIQFKCDNGRFYRRSVTLPRSCKC 4573
CP C C SV+C R VP + RL+L N I I K DF L L+VL L +N++S IE GAF +L L +LRLN+N +Q LP+ LF KL +LDL ENQ+Q + F + +K L L+ N + I + +T H+ R LR+ SN L+C+CHL WL+ +L+ + + + +C+ P L ++ + E+ C +E +C + CP CTCS + IV+C GK L IP +LP +E+ L+ NSI + G ++YK LK+I +++N + DI P AF GL+ LT+L L N + I + F+GL SL L L+ N+I CL TF+ ++ L LL L +NKL + + + PL ++ L+L NP CDC + WL L+ + T A C P + +++ F+C+ D + E C ++CP KC C IV+CS L +P + T L L+ N++ L+ + NL K++LSNN+I+ ++ F + ++E+ L+ N+L+ CV+N+TF L + +L L DN I+ I +F +L + L +N +C+C L WL +W R + G+ P CQ P LK P+ ++ + F C+GN EE+S C P+CP +C C T + CS L+ +P +P + TELYL+ N L + L +L + LS N I+ + + + +L TL+LS+N+L+CI AF L +L+VL L ND+S++P +F DLT+L+++ALG NP HCDC+++WL+++ + + GI+ C+SPE M + + + F C D I AKC+AC+ +PC+NNG C+ Y C CP + GK C IN C PC++GG C +D+ + + C CP GF G +C N DDC + CEN ATCVD +N Y C CP + G+ C+ +C + N C++ C PL + + C C G+ GK CE + DDC + C++ A+C D +NGY C C G+ G +CE P P L + S C +EC N C+ + +Q+ C+C PG++GP C+ L ++ F GKDS++E+ + + P+ NISL+ T+ +G++LY+G D L + L++GH+++ Y D P +S++ VND + H + + + + L + ++ +L L + +++ +++GG P +A++ F GCI + IN + DF +S+ V PGC S + P P D E + + S K +C N CH ++++ Y C+C+ GF G C++ + C G+ R I+ + +C + K C+G C CCQP++ K F+C +G + V C C
Sbjct: 34 CPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTV----GQFTLCMAPVHLRGFNVADVQKKEYVCPAPHSEPPSCNANSISCPSPCTCSNN-IVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIAKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIE-TSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRSRFSSE-CFMDLVCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIVSGN---PRCQKPFFLKEIPIQDVAIQDFTCDGN----EESS----------------CQLSPRCPEQCTCMETVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAV-PRELSALRHLTLIDLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPEPMADRLLLTTPTHRFQCKGPV-DINIVAKCNACLSSPCKNNGTCTQDPVELYRCACPYSYKGKDCTVPINTCIQNPCQHGGTCHLSDSHKDGFSCSCPLGFEGQRCEINPDDCEDNDCENNATCVDGINNYVCICPPNYTGELCDEVIDHCVPELNLCQHEAKCIPL-DKGFSCECVPGYSGKLCETDNDDCVAHKCRHGAQCVDTINGYTCTCPQGFSGPFCEHPP--PMVL------------------------LQTSPCDQYECQNGAQCI---VVQQEPTCRCPPGFAGPRCEKLITVNFVGKDSYVEL---ASAKVRPQANISLQVATDKDNGILLYKG-DNDPLALELYQGHVRLVY---DSLSSPPTTVYSVETVNDGQFHSVELVTLNQTLNLVVDKGTPKSLGK--LQKQPAVGINSPLYLGGIPTSTGLSALRQGTDRPLGGFHGCIHEVRINNELQDFKALPPQSLGVSPGCKSCTVCKHGLCRSVEKDSVVCECRPGWTGPLCDQEARDPCLGHRCHHGKCVATGTSYMCKCAEGYGGDLCDNKNDSANACSAFKCHHGQCHISDQGEPY-CLCQPGFSGEHCQQE-NPCLGQVVREVIRRQKGYASCATASKVPIMECRGGCGPQ---------------------CCQPTRSKRRKYVFQCTDGSSFVEEVERHLECGC 1519 HSP 2 Score: 723.391 bits (1866), Expect = 0.000e+0 Identity = 488/1580 (30.89%), Postives = 768/1580 (48.61%), Query Frame = -2
Query: 5058 CPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXIQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISEA----------------------ITKLKHL---RELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCA---NEENTCQPDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXLTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLK------------------------CVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGN-YECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYC-HDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNSLYF-GKDSHIEIHPPSRGFLMPKGNISLKFITNSSSGLILYQGFDKSYLVVNLFRGHIKISYNIGDKDIGPDG--FSMDVVNDNKLHYLNIEIVGENLTMYLNGKVHFTLKSKYLMTNQYMALDTGIFIGGAPKKILNTAIQNHHFDNDSDFTGCIESLLINRKKIDFSKYMSKSVNVKPGCGDLI-------------SKTAQELPSSEVPTQDIEVP-------------IKSTKSLPVKSTICSKPGVCSNNG---------VCH----EVNEKNRKYKCVCKNGFVGRKCKKRVSKCHGKTTRTFIKDPDTHDNCLSIKKFQYRVCKGTCSNNRMQADSNRNFYQRSTDNSLSPCCQPSKYTTHKIQFKCDNGRFYRRSVTLPRSCKC 9509
CP C C SV+C R VP + RL+L N I I K DF L L+VL L +N++S IE GAF +L L +LRLN+N +Q LP+ LF KL +LDL ENQ+Q + F + +K L L+ N + I + +T H+ R LR+ SN L+C+CHL WL+ +L+ + + + +C+ P L ++ + E+ C +E +C + CP CTCS + IV+C GK L IP +LP +E+ L+ NSI + G ++YK LK+I +++N + DI P AF GL+ LT+L L N + I + F+GL SL L L+ N+I CL TF+ ++ L LL L +NKL + + + PL ++ L+L NP CDC + WL L+ + T A C P + +++ F+C+ D + E C ++CP KC C IV+CS L +P + T L L+ N++ L+ + NL K++LSNN+I+ ++ F + ++E+ L+ N+L+ CV+N+TF L + +L L DN I+ I +F +L + L +N +C+C L WL +W R + G+ P CQ P LK P+ ++ + F C+GN EE+S C P+CP +C C T + CS L+ +P +P + TELYL+ N L + L +L + LS N I+ + + + +L TL+LS+N+L+CI AF L +L+VL L ND+S++P +F DLT+L+++ALG NP HCDC+++WL+++ + + GI+ C+SPE M + + + F C D I AKC+AC+ +PC+NNG C+ Y C CP + GK C IN C PC++GG C +D+ + + C CP GF G +C N DDC + CEN ATCVD +N Y C CP + G+ C+ +C + N C++ C PL + + C C G+ GK CE + DDC + C++ A+C D +NGY C C G+ G +CE P P L + S C +EC N C+ + +Q+ C+C PG++GP C+ L ++ F GKDS++E+ + + P+ NISL+ T+ +G++LY+G D L + L++GH+++ Y D P +S++ VND + H + + + + L + ++ +L L + +++ +++GG P +A++ F GCI + IN + DF +S+ V PGC S + P P D E + + S K +C N CH ++++ Y C+C+ GF G C++ + C G+ R I+ + +C + K C+G C CCQP++ K F+C +G + V C C
Sbjct: 34 CPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTV----GQFTLCMAPVHLRGFNVADVQKKEYVCPAPHSEPPSCNANSISCPSPCTCSNN-IVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIAKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIE-TSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRSRFSSE-CFMDLVCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIVSGN---PRCQKPFFLKEIPIQDVAIQDFTCDGN----EESS----------------CQLSPRCPEQCTCMETVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAV-PRELSALRHLTLIDLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPEPMADRLLLTTPTHRFQCKGPV-DINIVAKCNACLSSPCKNNGTCTQDPVELYRCACPYSYKGKDCTVPINTCIQNPCQHGGTCHLSDSHKDGFSCSCPLGFEGQRCEINPDDCEDNDCENNATCVDGINNYVCICPPNYTGELCDEVIDHCVPELNLCQHEAKCIPL-DKGFSCECVPGYSGKLCETDNDDCVAHKCRHGAQCVDTINGYTCTCPQGFSGPFCEHPP--PMVL------------------------LQTSPCDQYECQNGAQCI---VVQQEPTCRCPPGFAGPRCEKLITVNFVGKDSYVEL---ASAKVRPQANISLQVATDKDNGILLYKG-DNDPLALELYQGHVRLVY---DSLSSPPTTVYSVETVNDGQFHSVELVTLNQTLNLVVDKGTPKSLGK--LQKQPAVGINSPLYLGGIPTSTGLSALRQGTDRPLGGFHGCIHEVRINNELQDFKALPPQSLGVSPGCKSCTVCKHGLCRSVEKDSVVCECRPGWTGPLCDQEARDPCLGHRCHHGKCVATGTSYMCKCAEGYGGDLCDNKNDSANACSAFKCHHGQCHISDQGEPY-CLCQPGFSGEHCQQE-NPCLGQVVREVIRRQKGYASCATASKVPIMECRGGCGPQ---------------------CCQPTRSKRRKYVFQCTDGSSFVEEVERHLECGC 1519 HSP 3 Score: 281.952 bits (720), Expect = 2.349e-75 Identity = 216/739 (29.23%), Postives = 331/739 (44.79%), Query Frame = 2
Query: 773 PDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXXTQNGMVDIEPGAFIGLQQLTALYLNMNNLRT------------------------IRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVI-CPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINL------------------------SSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESS-FKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQS------------------------NDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDT-YITAKCD---ACMKT--PCQNNG 2749
P CP +CTCS V+C G L +P+ +P N LDL N+IT + + + K L+ + L N + IE GAF L+QL L LN N L+ I R AF G++ + L L NN I C+ G F ++ L +L L+ N +S + + K+R L L N LYCDC + WL L+ + C+ P G V +Q + C + + +C I CP C+CS+ IV+C G GL +P + + L N IK + + Q L ++D+S NQI +I PD F L + L ++NK+ C+ TF+ L L +L L DN + I F +++ L L N CDC LKWL+++ ++N R C SP L + + K F C G++ ++ FS C +D+ CP KC+CE T +DCS +L IP+ +P T+L L+ N++ L + FK L NL+ + LS N I E+ + +++ L+L+ N+L+ +H F+ LS LK L+L+S N ++TI AF L +L+ I L NPF+C+C++ WL ++ R + +G C P +K I +F C E + ++ +C CM+T C N G
Sbjct: 29 PPAVACPTKCTCSA-ASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTVGQ-FTLCMAPVHLRGFNVADVQKKEYVCPAP-----HSEPPSCNANSISCPSPCTCSNNIVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPA-GAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIAKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGAR---CSSPRRLANKRISQIKSKKFRCSGSED--------YRSRFSSECFMDL------VCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIVSGNPRCQKPFFLKEIPIQDVAIQDFTCDGNEESSCQLSPRCPEQCTCMETVVRCSNKG 742 HSP 4 Score: 281.952 bits (720), Expect = 2.349e-75 Identity = 216/739 (29.23%), Postives = 331/739 (44.79%), Query Frame = -2
Query: 6882 PDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXLTQNGMVDIEPGAFIGLQQLTALYLNMNNLRT------------------------IRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVI-CPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINL------------------------SSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESS-FKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQS------------------------NDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDT-YITAKCD---ACMKT--PCQNNG 8858
P CP +CTCS V+C G L +P+ +P N LDL N+IT + + + K L+ + L N + IE GAF L+QL L LN N L+ I R AF G++ + L L NN I C+ G F ++ L +L L+ N +S + + K+R L L N LYCDC + WL L+ + C+ P G V +Q + C + + +C I CP C+CS+ IV+C G GL +P + + L N IK + + Q L ++D+S NQI +I PD F L + L ++NK+ C+ TF+ L L +L L DN + I F +++ L L N CDC LKWL+++ ++N R C SP L + + K F C G++ ++ FS C +D+ CP KC+CE T +DCS +L IP+ +P T+L L+ N++ L + FK L NL+ + LS N I E+ + +++ L+L+ N+L+ +H F+ LS LK L+L+S N ++TI AF L +L+ I L NPF+C+C++ WL ++ R + +G C P +K I +F C E + ++ +C CM+T C N G
Sbjct: 29 PPAVACPTKCTCSA-ASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTVGQ-FTLCMAPVHLRGFNVADVQKKEYVCPAP-----HSEPPSCNANSISCPSPCTCSNNIVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPA-GAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIAKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGAR---CSSPRRLANKRISQIKSKKFRCSGSED--------YRSRFSSECFMDL------VCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIVSGNPRCQKPFFLKEIPIQDVAIQDFTCDGNEESSCQLSPRCPEQCTCMETVVRCSNKG 742 HSP 5 Score: 201.445 bits (511), Expect = 1.487e-50 Identity = 136/440 (30.91%), Postives = 217/440 (49.32%), Query Frame = 2
Query: 119 ICPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFF-KLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXXQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISE-AITKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQC-ANEENTCQPDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXXTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDI 1429
+CP+ C+CE V+C ++ +P+ L E T L L N + V+ + F KL L+ + LSNN+I + GAFD ++ +L L N ++ + +F LS L+ L LR N + C++ F+ L ++ L L N++ I+ A T L L + + SN +CNCHL WL +L+ K I + R C P L +IPI ++ +F C NEE++CQ + CP+QCTC + +V C+ K L +P+ +P + EL L+ N +T + P L+ LT + L+ N++ + F+ +S L L L N + C+P F G++ L +L L N +S++ E L L +L L NPL+CDC + WL + +K +A C P + + + FQC DI
Sbjct: 504 VCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLR-KRRIVSGNPR---CQKPFFLKEIPIQDVAIQDFTCDGNEESSCQLSPR-CPEQCTC-METVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAV-------------------------PRELSALRHLTLIDLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVK-AGYKEPGIARCSSPEPMADRLLLTTPTHRFQCKGPVDI 911 HSP 6 Score: 201.445 bits (511), Expect = 1.487e-50 Identity = 136/440 (30.91%), Postives = 217/440 (49.32%), Query Frame = -2
Query: 8202 ICPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFF-KLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXIQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISE-AITKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQC-ANEENTCQPDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXLTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDI 9512
+CP+ C+CE V+C ++ +P+ L E T L L N + V+ + F KL L+ + LSNN+I + GAFD ++ +L L N ++ + +F LS L+ L LR N + C++ F+ L ++ L L N++ I+ A T L L + + SN +CNCHL WL +L+ K I + R C P L +IPI ++ +F C NEE++CQ + CP+QCTC + +V C+ K L +P+ +P + EL L+ N +T + P L+ LT + L+ N++ + F+ +S L L L N + C+P F G++ L +L L N +S++ E L L +L L NPL+CDC + WL + +K +A C P + + + FQC DI
Sbjct: 504 VCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLR-KRRIVSGNPR---CQKPFFLKEIPIQDVAIQDFTCDGNEESSCQLSPR-CPEQCTC-METVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAV-------------------------PRELSALRHLTLIDLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVK-AGYKEPGIARCSSPEPMADRLLLTTPTHRFQCKGPVDI 911
BLAST of slit vs. RefSeq Human
Match: gi|429836873|ref|NP_001258875.1| (slit homolog 3 protein isoform 1 precursor [Homo sapiens]) HSP 1 Score: 723.391 bits (1866), Expect = 0.000e+0 Identity = 489/1587 (30.81%), Postives = 770/1587 (48.52%), Query Frame = 2
Query: 122 CPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXXQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISEA----------------------ITKLKHL---RELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCA---NEENTCQPDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXXTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLK------------------------CVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEF------ICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGN-YECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYC-HDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNSLYF-GKDSHIEIHPPSRGFLMPKGNISLKFITNSSSGLILYQGFDKSYLVVNLFRGHIKISYNIGDKDIGPDG--FSMDVVNDNKLHYLNIEIVGENLTMYLNGKVHFTLKSKYLMTNQYMALDTGIFIGGAPKKILNTAIQNHHFDNDSDFTGCIESLLINRKKIDFSKYMSKSVNVKPGCGDLI-------------SKTAQELPSSEVPTQDIEVP-------------IKSTKSLPVKSTICSKPGVCSN--------------NGVCHEVNEKNRKYKCVCKNGFVGRKCKKRVSKCHGKTTRTFIKDPDTHDNCLSIKKFQYRVCKGTCSNNRMQADSNRNFYQRSTDNSLSPCCQPSKYTTHKIQFKCDNGRFYRRSVTLPRSCKC 4573
CP C C SV+C R VP + RL+L N I I K DF L L+VL L +N++S IE GAF +L L +LRLN+N +Q LP+ LF KL +LDL ENQ+Q + F + +K L L+ N + I + +T H+ R LR+ SN L+C+CHL WL+ +L+ + + + +C+ P L ++ + E+ C +E +C + CP CTCS + IV+C GK L IP +LP +E+ L+ NSI + G ++YK LK+I +++N + DI P AF GL+ LT+L L N + I + F+GL SL L L+ N+I CL TF+ ++ L LL L +NKL + + + PL ++ L+L NP CDC + WL L+ + T A C P + +++ F+C+ D + E C ++CP KC C IV+CS L +P + T L L+ N++ L+ + NL K++LSNN+I+ ++ F + ++E+ L+ N+L+ CV+N+TF L + +L L DN I+ I +F +L + L +N +C+C L WL +W R + G+ P CQ P LK P+ ++ + F C+GN EE+S C P+CP +C C T + CS L+ +P +P + TELYL+ N L + L +L + LS N I+ + + + +L TL+LS+N+L+CI AF L +L+VL L ND+S++P +F DLT+L+++ALG NP HCDC+++WL+++ + + GI+ C+SPE M + + + F C D I AKC+AC+ +PC+NNG C+ Y C CP + GK C IN C PC++GG C +D+ + + C CP GF G +C N DDC + CEN ATCVD +N Y C CP + G+ C+ +C + N C++ C PL + + C C G+ GK CE + DDC + C++ A+C D +NGY C C G+ G +CE P P L + S C +EC N C+ + +Q+ C+C PG++GP C+ L ++ F GKDS++E+ + + P+ NISL+ T+ +G++LY+G D L + L++GH+++ Y D P +S++ VND + H + + + + L + ++ +L L + +++ +++GG P +A++ F GCI + IN + DF +S+ V PGC S + P P D E + + S K +C N +G CH ++++ Y C+C+ GF G C++ + C G+ R I+ + +C + K C+G C CCQP++ K F+C +G + V C C
Sbjct: 34 CPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTV----GQFTLCMAPVHLRGFNVADVQKKEYVCPAPHSEPPSCNANSISCPSPCTCSNN-IVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIAKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIE-TSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRSRFSSE-CFMDLVCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIVSGN---PRCQKPFFLKEIPIQDVAIQDFTCDGN----EESS----------------CQLSPRCPEQCTCMETVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAV-PRELSALRHLTLIDLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPEPMADRLLLTTPTHRFQCKVLWFCCPGPVDINIVAKCNACLSSPCKNNGTCTQDPVELYRCACPYSYKGKDCTVPINTCIQNPCQHGGTCHLSDSHKDGFSCSCPLGFEGQRCEINPDDCEDNDCENNATCVDGINNYVCICPPNYTGELCDEVIDHCVPELNLCQHEAKCIPL-DKGFSCECVPGYSGKLCETDNDDCVAHKCRHGAQCVDTINGYTCTCPQGFSGPFCEHPP--PMVL------------------------LQTSPCDQYECQNGAQCI---VVQQEPTCRCPPGFAGPRCEKLITVNFVGKDSYVEL---ASAKVRPQANISLQVATDKDNGILLYKG-DNDPLALELYQGHVRLVY---DSLSSPPTTVYSVETVNDGQFHSVELVTLNQTLNLVVDKGTPKSLGK--LQKQPAVGINSPLYLGGIPTSTGLSALRQGTDRPLGGFHGCIHEVRINNELQDFKALPPQSLGVSPGCKSCTVCKHGLCRSVEKDSVVCECRPGWTGPLCDQEARDPCLGHRCHHGKCVATGTSYMCKCAEGYGGDLCDNKNDSANACSAFKCHHGQCH-ISDQGEPY-CLCQPGFSGEHCQQE-NPCLGQVVREVIRRQKGYASCATASKVPIMECRGGCGPQ---------------------CCQPTRSKRRKYVFQCTDGSSFVEEVERHLECGC 1526 HSP 2 Score: 723.391 bits (1866), Expect = 0.000e+0 Identity = 489/1587 (30.81%), Postives = 770/1587 (48.52%), Query Frame = -2
Query: 5058 CPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXIQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISEA----------------------ITKLKHL---RELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCA---NEENTCQPDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXLTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLK------------------------CVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEF------ICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGN-YECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYC-HDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNSLYF-GKDSHIEIHPPSRGFLMPKGNISLKFITNSSSGLILYQGFDKSYLVVNLFRGHIKISYNIGDKDIGPDG--FSMDVVNDNKLHYLNIEIVGENLTMYLNGKVHFTLKSKYLMTNQYMALDTGIFIGGAPKKILNTAIQNHHFDNDSDFTGCIESLLINRKKIDFSKYMSKSVNVKPGCGDLI-------------SKTAQELPSSEVPTQDIEVP-------------IKSTKSLPVKSTICSKPGVCSN--------------NGVCHEVNEKNRKYKCVCKNGFVGRKCKKRVSKCHGKTTRTFIKDPDTHDNCLSIKKFQYRVCKGTCSNNRMQADSNRNFYQRSTDNSLSPCCQPSKYTTHKIQFKCDNGRFYRRSVTLPRSCKC 9509
CP C C SV+C R VP + RL+L N I I K DF L L+VL L +N++S IE GAF +L L +LRLN+N +Q LP+ LF KL +LDL ENQ+Q + F + +K L L+ N + I + +T H+ R LR+ SN L+C+CHL WL+ +L+ + + + +C+ P L ++ + E+ C +E +C + CP CTCS + IV+C GK L IP +LP +E+ L+ NSI + G ++YK LK+I +++N + DI P AF GL+ LT+L L N + I + F+GL SL L L+ N+I CL TF+ ++ L LL L +NKL + + + PL ++ L+L NP CDC + WL L+ + T A C P + +++ F+C+ D + E C ++CP KC C IV+CS L +P + T L L+ N++ L+ + NL K++LSNN+I+ ++ F + ++E+ L+ N+L+ CV+N+TF L + +L L DN I+ I +F +L + L +N +C+C L WL +W R + G+ P CQ P LK P+ ++ + F C+GN EE+S C P+CP +C C T + CS L+ +P +P + TELYL+ N L + L +L + LS N I+ + + + +L TL+LS+N+L+CI AF L +L+VL L ND+S++P +F DLT+L+++ALG NP HCDC+++WL+++ + + GI+ C+SPE M + + + F C D I AKC+AC+ +PC+NNG C+ Y C CP + GK C IN C PC++GG C +D+ + + C CP GF G +C N DDC + CEN ATCVD +N Y C CP + G+ C+ +C + N C++ C PL + + C C G+ GK CE + DDC + C++ A+C D +NGY C C G+ G +CE P P L + S C +EC N C+ + +Q+ C+C PG++GP C+ L ++ F GKDS++E+ + + P+ NISL+ T+ +G++LY+G D L + L++GH+++ Y D P +S++ VND + H + + + + L + ++ +L L + +++ +++GG P +A++ F GCI + IN + DF +S+ V PGC S + P P D E + + S K +C N +G CH ++++ Y C+C+ GF G C++ + C G+ R I+ + +C + K C+G C CCQP++ K F+C +G + V C C
Sbjct: 34 CPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTV----GQFTLCMAPVHLRGFNVADVQKKEYVCPAPHSEPPSCNANSISCPSPCTCSNN-IVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIAKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIE-TSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRSRFSSE-CFMDLVCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIVSGN---PRCQKPFFLKEIPIQDVAIQDFTCDGN----EESS----------------CQLSPRCPEQCTCMETVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAV-PRELSALRHLTLIDLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPEPMADRLLLTTPTHRFQCKVLWFCCPGPVDINIVAKCNACLSSPCKNNGTCTQDPVELYRCACPYSYKGKDCTVPINTCIQNPCQHGGTCHLSDSHKDGFSCSCPLGFEGQRCEINPDDCEDNDCENNATCVDGINNYVCICPPNYTGELCDEVIDHCVPELNLCQHEAKCIPL-DKGFSCECVPGYSGKLCETDNDDCVAHKCRHGAQCVDTINGYTCTCPQGFSGPFCEHPP--PMVL------------------------LQTSPCDQYECQNGAQCI---VVQQEPTCRCPPGFAGPRCEKLITVNFVGKDSYVEL---ASAKVRPQANISLQVATDKDNGILLYKG-DNDPLALELYQGHVRLVY---DSLSSPPTTVYSVETVNDGQFHSVELVTLNQTLNLVVDKGTPKSLGK--LQKQPAVGINSPLYLGGIPTSTGLSALRQGTDRPLGGFHGCIHEVRINNELQDFKALPPQSLGVSPGCKSCTVCKHGLCRSVEKDSVVCECRPGWTGPLCDQEARDPCLGHRCHHGKCVATGTSYMCKCAEGYGGDLCDNKNDSANACSAFKCHHGQCH-ISDQGEPY-CLCQPGFSGEHCQQE-NPCLGQVVREVIRRQKGYASCATASKVPIMECRGGCGPQ---------------------CCQPTRSKRRKYVFQCTDGSSFVEEVERHLECGC 1526 HSP 3 Score: 281.952 bits (720), Expect = 2.689e-75 Identity = 216/739 (29.23%), Postives = 331/739 (44.79%), Query Frame = 2
Query: 773 PDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXXTQNGMVDIEPGAFIGLQQLTALYLNMNNLRT------------------------IRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVI-CPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINL------------------------SSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESS-FKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQS------------------------NDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDT-YITAKCD---ACMKT--PCQNNG 2749
P CP +CTCS V+C G L +P+ +P N LDL N+IT + + + K L+ + L N + IE GAF L+QL L LN N L+ I R AF G++ + L L NN I C+ G F ++ L +L L+ N +S + + K+R L L N LYCDC + WL L+ + C+ P G V +Q + C + + +C I CP C+CS+ IV+C G GL +P + + L N IK + + Q L ++D+S NQI +I PD F L + L ++NK+ C+ TF+ L L +L L DN + I F +++ L L N CDC LKWL+++ ++N R C SP L + + K F C G++ ++ FS C +D+ CP KC+CE T +DCS +L IP+ +P T+L L+ N++ L + FK L NL+ + LS N I E+ + +++ L+L+ N+L+ +H F+ LS LK L+L+S N ++TI AF L +L+ I L NPF+C+C++ WL ++ R + +G C P +K I +F C E + ++ +C CM+T C N G
Sbjct: 29 PPAVACPTKCTCSA-ASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTVGQ-FTLCMAPVHLRGFNVADVQKKEYVCPAPH-----SEPPSCNANSISCPSPCTCSNNIVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPA-GAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIAKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGAR---CSSPRRLANKRISQIKSKKFRCSGSED--------YRSRFSSECFMDL------VCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIVSGNPRCQKPFFLKEIPIQDVAIQDFTCDGNEESSCQLSPRCPEQCTCMETVVRCSNKG 742 HSP 4 Score: 281.952 bits (720), Expect = 2.689e-75 Identity = 216/739 (29.23%), Postives = 331/739 (44.79%), Query Frame = -2
Query: 6882 PDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXLTQNGMVDIEPGAFIGLQQLTALYLNMNNLRT------------------------IRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVI-CPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINL------------------------SSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESS-FKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQS------------------------NDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDT-YITAKCD---ACMKT--PCQNNG 8858
P CP +CTCS V+C G L +P+ +P N LDL N+IT + + + K L+ + L N + IE GAF L+QL L LN N L+ I R AF G++ + L L NN I C+ G F ++ L +L L+ N +S + + K+R L L N LYCDC + WL L+ + C+ P G V +Q + C + + +C I CP C+CS+ IV+C G GL +P + + L N IK + + Q L ++D+S NQI +I PD F L + L ++NK+ C+ TF+ L L +L L DN + I F +++ L L N CDC LKWL+++ ++N R C SP L + + K F C G++ ++ FS C +D+ CP KC+CE T +DCS +L IP+ +P T+L L+ N++ L + FK L NL+ + LS N I E+ + +++ L+L+ N+L+ +H F+ LS LK L+L+S N ++TI AF L +L+ I L NPF+C+C++ WL ++ R + +G C P +K I +F C E + ++ +C CM+T C N G
Sbjct: 29 PPAVACPTKCTCSA-ASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTVGQ-FTLCMAPVHLRGFNVADVQKKEYVCPAPH-----SEPPSCNANSISCPSPCTCSNNIVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPA-GAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIAKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGAR---CSSPRRLANKRISQIKSKKFRCSGSED--------YRSRFSSECFMDL------VCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIVSGNPRCQKPFFLKEIPIQDVAIQDFTCDGNEESSCQLSPRCPEQCTCMETVVRCSNKG 742 HSP 5 Score: 198.364 bits (503), Expect = 1.365e-49 Identity = 134/434 (30.88%), Postives = 215/434 (49.54%), Query Frame = 2
Query: 119 ICPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFF-KLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXXQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISE-AITKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQC-ANEENTCQPDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXXTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQC 1411
+CP+ C+CE V+C ++ +P+ L E T L L N + V+ + F KL L+ + LSNN+I + GAFD ++ +L L N ++ + +F LS L+ L LR N + C++ F+ L ++ L L N++ I+ A T L L + + SN +CNCHL WL +L+ K I + R C P L +IPI ++ +F C NEE++CQ + CP+QCTC + +V C+ K L +P+ +P + EL L+ N +T + P L+ LT + L+ N++ + F+ +S L L L N + C+P F G++ L +L L N +S++ E L L +L L NPL+CDC + WL + +K +A C P + + + FQC
Sbjct: 504 VCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLR-KRRIVSGNPR---CQKPFFLKEIPIQDVAIQDFTCDGNEESSCQLSPR-CPEQCTC-METVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAV-------------------------PRELSALRHLTLIDLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVK-AGYKEPGIARCSSPEPMADRLLLTTPTHRFQC 905 HSP 6 Score: 198.364 bits (503), Expect = 1.365e-49 Identity = 134/434 (30.88%), Postives = 215/434 (49.54%), Query Frame = -2
Query: 8220 ICPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFF-KLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXIQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISE-AITKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQC-ANEENTCQPDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXLTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQC 9512
+CP+ C+CE V+C ++ +P+ L E T L L N + V+ + F KL L+ + LSNN+I + GAFD ++ +L L N ++ + +F LS L+ L LR N + C++ F+ L ++ L L N++ I+ A T L L + + SN +CNCHL WL +L+ K I + R C P L +IPI ++ +F C NEE++CQ + CP+QCTC + +V C+ K L +P+ +P + EL L+ N +T + P L+ LT + L+ N++ + F+ +S L L L N + C+P F G++ L +L L N +S++ E L L +L L NPL+CDC + WL + +K +A C P + + + FQC
Sbjct: 504 VCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLR-KRRIVSGNPR---CQKPFFLKEIPIQDVAIQDFTCDGNEESSCQLSPR-CPEQCTC-METVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAV-------------------------PRELSALRHLTLIDLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVK-AGYKEPGIARCSSPEPMADRLLLTTPTHRFQC 905
BLAST of slit vs. RefSeq Human
Match: gi|574281382|ref|NP_001276065.1| (slit homolog 2 protein isoform 3 precursor [Homo sapiens]) HSP 1 Score: 707.212 bits (1824), Expect = 0.000e+0 Identity = 469/1476 (31.78%), Postives = 732/1476 (49.59%), Query Frame = 2
Query: 116 EICPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXXQHLPDHLFSKLSKLQKLDLRENQLQ------------------------CLNEHVFSKLGMLKTLLLEGNKLYWISEA-ITKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCANEENTCQPDRKV--CPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXXTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNEL------------------------HCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGIC--KNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYC-HDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNSLYF-GKDSHIEIHPPSRGFLMPKGNISLKFITNSSSGLILYQGFDKSYLVVNLFRGHIKISYNIGDKDIGPDGFSMDVVNDNKLHYLNIEIVGENLTMYLNGKVHFTLKSKYLMTNQYMALDTGIFIGGAPKKILNTAIQNHHFDNDSDFTGCIESLLINRKKIDFSKYMSKSVNVKPGCGDLISKTAQELPSSEVPTQDIEVPIKSTKSLPVKSTICSKPGVCSNNGVCHEVNEKNRKYKCVCKNGFVGRKCKKRVSKCHGKTTRTFIKDPDTHDNCLSIKKFQY 4378
+ CP C C +V+C R+VP + T RL+L GN I I K+DF L +L+VLQL N+IS+IE GAF +L L +LRLNRN +Q P+ LF +KL +LDL ENQ+Q C+ + F L L+ L L N + +S A + LR R+ SN L+C+CHL WL+ +L+ + + C+ P L ++ + EF C+ ++ P V CP CTCS + IV+C GK LT IP +LP E+ L+ N+I ++ G S YK L++I L+ N + ++ P AF GL+ L +L L N + + ++ F GL SL L L+ N+I CL F+ + L LL L +NKL + + PL ++ ++L NP CDC + WL L + T A C P + +++ F+C+ D + + C + CP KC C V+CS L+ +PE I T L L++N+ L+ + L K++ SNN+I +I+ F ++ + EI L+SN+L+ V ++ FK L L+ L L N I+C+G +SF ++++ L L +N++ +C+C L WL EW R + G+ P CQ P LK P+ ++ + F C+ D C+ + +CP++C C T + CS LK +P IP + TELYLD N TL N +L + LS N I+ ++ + + L TL+LS+N+L+CI F L +L++L L ND+S +P AF DL+ L+++A+G NP +CDCN++WL+ + + + GI+ CA P M K + + +F C D I AKC+ C+ PC+N+G C+ Y C CP F G+ C+ I+AC PC++GG C K + G + C+C GF G C N+DDC + CEN +TCVD +N Y+C CP + G+ CE K +C D NPC++ C L+ +KC CT G+ G++C+ + DDC+ N CKN A C D VNGY CIC GY G +CE F+ + S C +C N C+ + + C+C PGY G C+ L S+ F K+S+++I P ++ + P+ NI+L+ T+ SG++LY+G DK ++ V L+RG ++ SY+ G +S++ +ND H + + + ++L++ ++G + + L + D+ +++GG P K +++ N + F GCI +L IN + DF K + + PGC P +C+ +G C ++ + C C+ G++G C +R T + + H CL I F Y
Sbjct: 26 QACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV----GLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCSVLHCPAACTCSNN-IVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIE-TSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGD-CFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGN---PRCQKPYFLKEIPIQDVAIQDFTCDDGND-------------------DNSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQF-TLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPV-DVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEEDG-FWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKC-ILTPKGFKCDCTPGYVGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCICPEGYSGLFCE--------------------------FSPPMVLPRTSPCDNFDCQNGAQCI---VRINEPICQCLPGYQGEKCEKLVSVNFINKESYLQI-PSAK--VRPQTNITLQIATDEDSGILLYKG-DKDHIAVELYRGRVRASYDTGSHPASAI-YSVETINDGNFHIVELLALDQSLSLSVDGGNPKIITN--LSKQSTLNFDSPLYVGGMPGKSNVASLRQAPGQNGTSFHGCIRNLYINSELQDFQK-VPMQTGILPGCE------------------------------PCHKKVCA-------HGTCQPSSQAG--FTCECQEGWMGPLCDQR-------TNDPCLGNKCVHGTCLPINAFSY 1385 HSP 2 Score: 707.212 bits (1824), Expect = 0.000e+0 Identity = 469/1476 (31.78%), Postives = 732/1476 (49.59%), Query Frame = -2
Query: 5253 EICPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXIQHLPDHLFSKLSKLQKLDLRENQLQ------------------------CLNEHVFSKLGMLKTLLLEGNKLYWISEA-ITKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCANEENTCQPDRKV--CPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXLTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNEL------------------------HCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGIC--KNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYC-HDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNSLYF-GKDSHIEIHPPSRGFLMPKGNISLKFITNSSSGLILYQGFDKSYLVVNLFRGHIKISYNIGDKDIGPDGFSMDVVNDNKLHYLNIEIVGENLTMYLNGKVHFTLKSKYLMTNQYMALDTGIFIGGAPKKILNTAIQNHHFDNDSDFTGCIESLLINRKKIDFSKYMSKSVNVKPGCGDLISKTAQELPSSEVPTQDIEVPIKSTKSLPVKSTICSKPGVCSNNGVCHEVNEKNRKYKCVCKNGFVGRKCKKRVSKCHGKTTRTFIKDPDTHDNCLSIKKFQY 9515
+ CP C C +V+C R+VP + T RL+L GN I I K+DF L +L+VLQL N+IS+IE GAF +L L +LRLNRN +Q P+ LF +KL +LDL ENQ+Q C+ + F L L+ L L N + +S A + LR R+ SN L+C+CHL WL+ +L+ + + C+ P L ++ + EF C+ ++ P V CP CTCS + IV+C GK LT IP +LP E+ L+ N+I ++ G S YK L++I L+ N + ++ P AF GL+ L +L L N + + ++ F GL SL L L+ N+I CL F+ + L LL L +NKL + + PL ++ ++L NP CDC + WL L + T A C P + +++ F+C+ D + + C + CP KC C V+CS L+ +PE I T L L++N+ L+ + L K++ SNN+I +I+ F ++ + EI L+SN+L+ V ++ FK L L+ L L N I+C+G +SF ++++ L L +N++ +C+C L WL EW R + G+ P CQ P LK P+ ++ + F C+ D C+ + +CP++C C T + CS LK +P IP + TELYLD N TL N +L + LS N I+ ++ + + L TL+LS+N+L+CI F L +L++L L ND+S +P AF DL+ L+++A+G NP +CDCN++WL+ + + + GI+ CA P M K + + +F C D I AKC+ C+ PC+N+G C+ Y C CP F G+ C+ I+AC PC++GG C K + G + C+C GF G C N+DDC + CEN +TCVD +N Y+C CP + G+ CE K +C D NPC++ C L+ +KC CT G+ G++C+ + DDC+ N CKN A C D VNGY CIC GY G +CE F+ + S C +C N C+ + + C+C PGY G C+ L S+ F K+S+++I P ++ + P+ NI+L+ T+ SG++LY+G DK ++ V L+RG ++ SY+ G +S++ +ND H + + + ++L++ ++G + + L + D+ +++GG P K +++ N + F GCI +L IN + DF K + + PGC P +C+ +G C ++ + C C+ G++G C +R T + + H CL I F Y
Sbjct: 26 QACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV----GLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCSVLHCPAACTCSNN-IVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIE-TSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGD-CFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGN---PRCQKPYFLKEIPIQDVAIQDFTCDDGND-------------------DNSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQF-TLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPV-DVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEEDG-FWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKC-ILTPKGFKCDCTPGYVGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCICPEGYSGLFCE--------------------------FSPPMVLPRTSPCDNFDCQNGAQCI---VRINEPICQCLPGYQGEKCEKLVSVNFINKESYLQI-PSAK--VRPQTNITLQIATDEDSGILLYKG-DKDHIAVELYRGRVRASYDTGSHPASAI-YSVETINDGNFHIVELLALDQSLSLSVDGGNPKIITN--LSKQSTLNFDSPLYVGGMPGKSNVASLRQAPGQNGTSFHGCIRNLYINSELQDFQK-VPMQTGILPGCE------------------------------PCHKKVCA-------HGTCQPSSQAG--FTCECQEGWMGPLCDQR-------TNDPCLGNKCVHGTCLPINAFSY 1385 HSP 3 Score: 293.123 bits (749), Expect = 7.628e-79 Identity = 223/694 (32.13%), Postives = 332/694 (47.84%), Query Frame = 2
Query: 101 PSEEVEICPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXXQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISEA-ITKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCANEEN-------TCQPDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQ-GFXXXXXXXXXXXXTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKAN--FSYSCCLDMKCNKIPKCPSKCKC 2149
PS V CP C C N V+C+ + +P L E T + L N I VI F L+ + LSNN+IS + AF L +L L L N I LP LF L LQ L L N++ CL F L L L L NKL I++ + L+ ++ + ++ N C+CHL WL +L +T C +P+RL+ I + + +F+C+ E+ C D CP++C C + V+C+ ++L IP+ +P T EL L NN T+L G K L+KI + N + DIE GAF G + + L N L ++ F GL SL L L +N I C+ +F G+ + LL L +N+++ + L L L L+ NP C+C + WL + L+ K + T C P I ++ + +F C+ +D D +C P CP +C+C +V CS GL LP+ I T L L N + + S ++ +L +DLSNN+I + +FS T+L + LS N+L+C+ TF L L +L L+ N IS + +F + L +L + N L+CDC ++WLS+W ++ Y G R C P + L K F C+G + + AK N S C D CN P +C C
Sbjct: 266 PSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNP----IETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDCFADL-ACPEKCRC-EGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRI-VTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGND------DNSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQFTLVPKELSNYK--HLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIAR---CAGPGEMADKLLLTTPSKKFTCQG----PVDVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTC 937 HSP 4 Score: 293.123 bits (749), Expect = 7.628e-79 Identity = 223/694 (32.13%), Postives = 332/694 (47.84%), Query Frame = -2
Query: 7482 PSEEVEICPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXIQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISEA-ITKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCANEEN-------TCQPDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQ-GFXXXXXXXXXXXLTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKAN--FSYSCCLDMKCNKIPKCPSKCKC 9530
PS V CP C C N V+C+ + +P L E T + L N I VI F L+ + LSNN+IS + AF L +L L L N I LP LF L LQ L L N++ CL F L L L L NKL I++ + L+ ++ + ++ N C+CHL WL +L +T C +P+RL+ I + + +F+C+ E+ C D CP++C C + V+C+ ++L IP+ +P T EL L NN T+L G K L+KI + N + DIE GAF G + + L N L ++ F GL SL L L +N I C+ +F G+ + LL L +N+++ + L L L L+ NP C+C + WL + L+ K + T C P I ++ + +F C+ +D D +C P CP +C+C +V CS GL LP+ I T L L N + + S ++ +L +DLSNN+I + +FS T+L + LS N+L+C+ TF L L +L L+ N IS + +F + L +L + N L+CDC ++WLS+W ++ Y G R C P + L K F C+G + + AK N S C D CN P +C C
Sbjct: 266 PSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNP----IETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDCFADL-ACPEKCRC-EGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRI-VTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGND------DNSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQFTLVPKELSNYK--HLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIAR---CAGPGEMADKLLLTTPSKKFTCQG----PVDVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTC 937 HSP 5 Score: 208.379 bits (529), Expect = 1.185e-52 Identity = 143/501 (28.54%), Postives = 225/501 (44.91%), Query Frame = 2
Query: 1451 NCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNL------------------DQIYSVHQA-----------------------------SNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVL------------------------SFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETED--TYITAKCDACMKTP 2734
N V CP +CSCS V+C G+ L S+P I +T L L+ N+I + ++I ++ + + L +LDLS NQI+ I F + +K + L N++ C+ + F+ L +LE+L LN+N+I+ + SF L+ L++N L+CDC L WLS+W R + C P L+G + + ++ FVC G++S + S + CP+ C C + +DC L EIPT +P TE+ L++N +K + +F L+ + LS N I+E+ D L++L +LVL + NK+ C+ DAF+DL NL +L L N + TI F L + + L QNPF CDC++KWL + ++ + C SP R+ K I K +F CS TED + ++ C A + P
Sbjct: 21 NKVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCS------------------VLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCS-GTEDYRSKLSGDCFADLACP 499 HSP 6 Score: 208.379 bits (529), Expect = 1.185e-52 Identity = 143/501 (28.54%), Postives = 225/501 (44.91%), Query Frame = -2
Query: 6897 NCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNL------------------DQIYSVHQA-----------------------------SNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVL------------------------SFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETED--TYITAKCDACMKTP 8180
N V CP +CSCS V+C G+ L S+P I +T L L+ N+I + ++I ++ + + L +LDLS NQI+ I F + +K + L N++ C+ + F+ L +LE+L LN+N+I+ + SF L+ L++N L+CDC L WLS+W R + C P L+G + + ++ FVC G++S + S + CP+ C C + +DC L EIPT +P TE+ L++N +K + +F L+ + LS N I+E+ D L++L +LVL + NK+ C+ DAF+DL NL +L L N + TI F L + + L QNPF CDC++KWL + ++ + C SP R+ K I K +F CS TED + ++ C A + P
Sbjct: 21 NKVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCS------------------VLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCS-GTEDYRSKLSGDCFADLACP 499
BLAST of slit vs. RefSeq Human
Match: gi|574281850|ref|NP_001276064.1| (slit homolog 2 protein isoform 2 precursor [Homo sapiens]) HSP 1 Score: 706.442 bits (1822), Expect = 0.000e+0 Identity = 472/1480 (31.89%), Postives = 734/1480 (49.59%), Query Frame = 2
Query: 116 EICPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXXQHLPDHLFSKLSKLQKLDLRENQLQ------------------------CLNEHVFSKLGMLKTLLLEGNKLYWISEA-ITKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCANEENTCQ----PDRKV--CPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXXTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNEL------------------------HCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGIC--KNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYC-HDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNSLYF-GKDSHIEIHPPSRGFLMPKGNISLKFITNSSSGLILYQGFDKSYLVVNLFRGHIKISYNIGDKDIGPDGFSMDVVNDNKLHYLNIEIVGENLTMYLNGKVHFTLKSKYLMTNQYMALDTGIFIGGAPKKILNTAIQNHHFDNDSDFTGCIESLLINRKKIDFSKYMSKSVNVKPGCGDLISKTAQELPSSEVPTQDIEVPIKSTKSLPVKSTICSKPGVCSNNGVCHEVNEKNRKYKCVCKNGFVGRKCKKRVSKCHGKTTRTFIKDPDTHDNCLSIKKFQY 4378
+ CP C C +V+C R+VP + T RL+L GN I I K+DF L +L+VLQL N+IS+IE GAF +L L +LRLNRN +Q P+ LF +KL +LDL ENQ+Q C+ + F L L+ L L N + +S A + LR R+ SN L+C+CHL WL+ +L+ + + C+ P L ++ + EF C++EE Q P V CP CTCS + IV+C GK LT IP +LP E+ L+ N+I ++ G S YK L++I L+ N + ++ P AF GL+ L +L L N + + ++ F GL SL L L+ N+I CL F+ + L LL L +NKL + + PL ++ ++L NP CDC + WL L + T A C P + +++ F+C+ D + + C + CP KC C V+CS L+ +PE I T L L++N+ L+ + L K++ SNN+I +I+ F ++ + EI L+SN+L+ V ++ FK L L+ L L N I+C+G +SF ++++ L L +N++ +C+C L WL EW R + G+ P CQ P LK P+ ++ + F C+ D C+ + +CP++C C T + CS LK +P IP + TELYLD N TL N +L + LS N I+ ++ + + L TL+LS+N+L+CI F L +L++L L ND+S +P AF DL+ L+++A+G NP +CDCN++WL+ + + + GI+ CA P M K + + +F C D I AKC+ C+ PC+N+G C+ Y C CP F G+ C+ I+AC PC++GG C K + G + C+C GF G C N+DDC + CEN +TCVD +N Y+C CP + G+ CE K +C D NPC++ C L+ +KC CT G+ G++C+ + DDC+ N CKN A C D VNGY CIC GY G +CE F+ + S C +C N C+ + + C+C PGY G C+ L S+ F K+S+++I P ++ + P+ NI+L+ T+ SG++LY+G DK ++ V L+RG ++ SY+ G +S++ +ND H + + + ++L++ ++G + + L + D+ +++GG P K +++ N + F GCI +L IN + DF K + + PGC P +C+ +G C ++ + C C+ G++G C +R T + + H CL I F Y
Sbjct: 26 QACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV----GLYTQCMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAACTCSNN-IVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIE-TSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGD-CFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGN---PRCQKPYFLKEIPIQDVAIQDFTCDDGND-------------------DNSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQF-TLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPV-DVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEEDG-FWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKC-ILTPKGFKCDCTPGYVGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCICPEGYSGLFCE--------------------------FSPPMVLPRTSPCDNFDCQNGAQCI---VRINEPICQCLPGYQGEKCEKLVSVNFINKESYLQI-PSAK--VRPQTNITLQIATDEDSGILLYKG-DKDHIAVELYRGRVRASYDTGSHPASAI-YSVETINDGNFHIVELLALDQSLSLSVDGGNPKIITN--LSKQSTLNFDSPLYVGGMPGKSNVASLRQAPGQNGTSFHGCIRNLYINSELQDFQK-VPMQTGILPGCE------------------------------PCHKKVCA-------HGTCQPSSQAG--FTCECQEGWMGPLCDQR-------TNDPCLGNKCVHGTCLPINAFSY 1389 HSP 2 Score: 706.442 bits (1822), Expect = 0.000e+0 Identity = 472/1480 (31.89%), Postives = 734/1480 (49.59%), Query Frame = -2
Query: 5253 EICPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXIQHLPDHLFSKLSKLQKLDLRENQLQ------------------------CLNEHVFSKLGMLKTLLLEGNKLYWISEA-ITKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCANEENTCQ----PDRKV--CPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXLTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNEL------------------------HCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGIC--KNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYC-HDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNSLYF-GKDSHIEIHPPSRGFLMPKGNISLKFITNSSSGLILYQGFDKSYLVVNLFRGHIKISYNIGDKDIGPDGFSMDVVNDNKLHYLNIEIVGENLTMYLNGKVHFTLKSKYLMTNQYMALDTGIFIGGAPKKILNTAIQNHHFDNDSDFTGCIESLLINRKKIDFSKYMSKSVNVKPGCGDLISKTAQELPSSEVPTQDIEVPIKSTKSLPVKSTICSKPGVCSNNGVCHEVNEKNRKYKCVCKNGFVGRKCKKRVSKCHGKTTRTFIKDPDTHDNCLSIKKFQY 9515
+ CP C C +V+C R+VP + T RL+L GN I I K+DF L +L+VLQL N+IS+IE GAF +L L +LRLNRN +Q P+ LF +KL +LDL ENQ+Q C+ + F L L+ L L N + +S A + LR R+ SN L+C+CHL WL+ +L+ + + C+ P L ++ + EF C++EE Q P V CP CTCS + IV+C GK LT IP +LP E+ L+ N+I ++ G S YK L++I L+ N + ++ P AF GL+ L +L L N + + ++ F GL SL L L+ N+I CL F+ + L LL L +NKL + + PL ++ ++L NP CDC + WL L + T A C P + +++ F+C+ D + + C + CP KC C V+CS L+ +PE I T L L++N+ L+ + L K++ SNN+I +I+ F ++ + EI L+SN+L+ V ++ FK L L+ L L N I+C+G +SF ++++ L L +N++ +C+C L WL EW R + G+ P CQ P LK P+ ++ + F C+ D C+ + +CP++C C T + CS LK +P IP + TELYLD N TL N +L + LS N I+ ++ + + L TL+LS+N+L+CI F L +L++L L ND+S +P AF DL+ L+++A+G NP +CDCN++WL+ + + + GI+ CA P M K + + +F C D I AKC+ C+ PC+N+G C+ Y C CP F G+ C+ I+AC PC++GG C K + G + C+C GF G C N+DDC + CEN +TCVD +N Y+C CP + G+ CE K +C D NPC++ C L+ +KC CT G+ G++C+ + DDC+ N CKN A C D VNGY CIC GY G +CE F+ + S C +C N C+ + + C+C PGY G C+ L S+ F K+S+++I P ++ + P+ NI+L+ T+ SG++LY+G DK ++ V L+RG ++ SY+ G +S++ +ND H + + + ++L++ ++G + + L + D+ +++GG P K +++ N + F GCI +L IN + DF K + + PGC P +C+ +G C ++ + C C+ G++G C +R T + + H CL I F Y
Sbjct: 26 QACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV----GLYTQCMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAACTCSNN-IVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIE-TSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGD-CFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGN---PRCQKPYFLKEIPIQDVAIQDFTCDDGND-------------------DNSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQF-TLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPV-DVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEEDG-FWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKC-ILTPKGFKCDCTPGYVGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCICPEGYSGLFCE--------------------------FSPPMVLPRTSPCDNFDCQNGAQCI---VRINEPICQCLPGYQGEKCEKLVSVNFINKESYLQI-PSAK--VRPQTNITLQIATDEDSGILLYKG-DKDHIAVELYRGRVRASYDTGSHPASAI-YSVETINDGNFHIVELLALDQSLSLSVDGGNPKIITN--LSKQSTLNFDSPLYVGGMPGKSNVASLRQAPGQNGTSFHGCIRNLYINSELQDFQK-VPMQTGILPGCE------------------------------PCHKKVCA-------HGTCQPSSQAG--FTCECQEGWMGPLCDQR-------TNDPCLGNKCVHGTCLPINAFSY 1389 HSP 3 Score: 293.123 bits (749), Expect = 8.039e-79 Identity = 223/694 (32.13%), Postives = 332/694 (47.84%), Query Frame = 2
Query: 101 PSEEVEICPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXXQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISEA-ITKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCANEEN-------TCQPDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQ-GFXXXXXXXXXXXXTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKAN--FSYSCCLDMKCNKIPKCPSKCKC 2149
PS V CP C C N V+C+ + +P L E T + L N I VI F L+ + LSNN+IS + AF L +L L L N I LP LF L LQ L L N++ CL F L L L L NKL I++ + L+ ++ + ++ N C+CHL WL +L +T C +P+RL+ I + + +F+C+ E+ C D CP++C C + V+C+ ++L IP+ +P T EL L NN T+L G K L+KI + N + DIE GAF G + + L N L ++ F GL SL L L +N I C+ +F G+ + LL L +N+++ + L L L L+ NP C+C + WL + L+ K + T C P I ++ + +F C+ +D D +C P CP +C+C +V CS GL LP+ I T L L N + + S ++ +L +DLSNN+I + +FS T+L + LS N+L+C+ TF L L +L L+ N IS + +F + L +L + N L+CDC ++WLS+W ++ Y G R C P + L K F C+G + + AK N S C D CN P +C C
Sbjct: 270 PSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNP----IETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDCFADL-ACPEKCRC-EGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRI-VTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGND------DNSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQFTLVPKELSNYK--HLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIAR---CAGPGEMADKLLLTTPSKKFTCQG----PVDVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTC 941 HSP 4 Score: 293.123 bits (749), Expect = 8.039e-79 Identity = 223/694 (32.13%), Postives = 332/694 (47.84%), Query Frame = -2
Query: 7482 PSEEVEICPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXIQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISEA-ITKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCANEEN-------TCQPDRKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQ-GFXXXXXXXXXXXLTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKAN--FSYSCCLDMKCNKIPKCPSKCKC 9530
PS V CP C C N V+C+ + +P L E T + L N I VI F L+ + LSNN+IS + AF L +L L L N I LP LF L LQ L L N++ CL F L L L L NKL I++ + L+ ++ + ++ N C+CHL WL +L +T C +P+RL+ I + + +F+C+ E+ C D CP++C C + V+C+ ++L IP+ +P T EL L NN T+L G K L+KI + N + DIE GAF G + + L N L ++ F GL SL L L +N I C+ +F G+ + LL L +N+++ + L L L L+ NP C+C + WL + L+ K + T C P I ++ + +F C+ +D D +C P CP +C+C +V CS GL LP+ I T L L N + + S ++ +L +DLSNN+I + +FS T+L + LS N+L+C+ TF L L +L L+ N IS + +F + L +L + N L+CDC ++WLS+W ++ Y G R C P + L K F C+G + + AK N S C D CN P +C C
Sbjct: 270 PSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNP----IETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDCFADL-ACPEKCRC-EGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRI-VTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGND------DNSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQFTLVPKELSNYK--HLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIAR---CAGPGEMADKLLLTTPSKKFTCQG----PVDVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTC 941 HSP 5 Score: 206.068 bits (523), Expect = 5.029e-52 Identity = 145/501 (28.94%), Postives = 226/501 (45.11%), Query Frame = 2
Query: 1451 NCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNL------------------DQIYSVHQA-----------------------------SNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVL------------------------SFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETED--TYITAKCDACMKTP 2734
N V CP +CSCS V+C G+ L S+P I +T L L+ N+I + ++I ++ + + L +LDLS NQI+ I F + +K + L N++ C+ + F+ L +LE+L LN+N+I+ + SF L+ L++N L+CDC L WLS+W R + C P L+G + + ++ FVC S +EE +F C + CP+ C C + +DC L EIPT +P TE+ L++N +K + +F L+ + LS N I+E+ D L++L +LVL + NK+ C+ DAF+DL NL +L L N + TI F L + + L QNPF CDC++KWL + ++ + C SP R+ K I K +F CS TED + ++ C A + P
Sbjct: 21 NKVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQCMGPSHLRGHNVAEVQKREFVC----SDEEE----GHQSFMAPSC------SVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCS-GTEDYRSKLSGDCFADLACP 503 HSP 6 Score: 206.068 bits (523), Expect = 5.029e-52 Identity = 145/501 (28.94%), Postives = 226/501 (45.11%), Query Frame = -2
Query: 6897 NCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNL------------------DQIYSVHQA-----------------------------SNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVL------------------------SFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETED--TYITAKCDACMKTP 8180
N V CP +CSCS V+C G+ L S+P I +T L L+ N+I + ++I ++ + + L +LDLS NQI+ I F + +K + L N++ C+ + F+ L +LE+L LN+N+I+ + SF L+ L++N L+CDC L WLS+W R + C P L+G + + ++ FVC S +EE +F C + CP+ C C + +DC L EIPT +P TE+ L++N +K + +F L+ + LS N I+E+ D L++L +LVL + NK+ C+ DAF+DL NL +L L N + TI F L + + L QNPF CDC++KWL + ++ + C SP R+ K I K +F CS TED + ++ C A + P
Sbjct: 21 NKVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQCMGPSHLRGHNVAEVQKREFVC----SDEEE----GHQSFMAPSC------SVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCS-GTEDYRSKLSGDCFADLACP 503
BLAST of slit vs. RefSeq Human
Match: gi|4759146|ref|NP_004778.1| (slit homolog 2 protein isoform 1 precursor [Homo sapiens]) HSP 1 Score: 702.205 bits (1811), Expect = 0.000e+0 Identity = 471/1483 (31.76%), Postives = 734/1483 (49.49%), Query Frame = 2
Query: 116 EICPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXXQHLPDHLFSKLSKLQKLDLRENQLQ------------------------CLNEHVFSKLGMLKTLLLEGNKLYWISEA-ITKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCANEENTCQPDRKV--CPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXXTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNS-ESDIKYDTHD------ENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNEL------------------------HCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGIC--KNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYC-HDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNSLYF-GKDSHIEIHPPSRGFLMPKGNISLKFITNSSSGLILYQGFDKSYLVVNLFRGHIKISYNIGDKDIGPDGFSMDVVNDNKLHYLNIEIVGENLTMYLNGKVHFTLKSKYLMTNQYMALDTGIFIGGAPKKILNTAIQNHHFDNDSDFTGCIESLLINRKKIDFSKYMSKSVNVKPGCGDLISKTAQELPSSEVPTQDIEVPIKSTKSLPVKSTICSKPGVCSNNGVCHEVNEKNRKYKCVCKNGFVGRKCKKRVSKCHGKTTRTFIKDPDTHDNCLSIKKFQY 4378
+ CP C C +V+C R+VP + T RL+L GN I I K+DF L +L+VLQL N+IS+IE GAF +L L +LRLNRN +Q P+ LF +KL +LDL ENQ+Q C+ + F L L+ L L N + +S A + LR R+ SN L+C+CHL WL+ +L+ + + C+ P L ++ + EF C+ ++ P V CP CTCS + IV+C GK LT IP +LP E+ L+ N+I ++ G S YK L++I L+ N + ++ P AF GL+ L +L L N + + ++ F GL SL L L+ N+I CL F+ + L LL L +NKL + + PL ++ ++L NP CDC + WL L + T A C P + +++ F+C++ E T D +C + CP KC C V+CS L+ +PE I T L L++N+ L+ + L K++ SNN+I +I+ F ++ + EI L+SN+L+ V ++ FK L L+ L L N I+C+G +SF ++++ L L +N++ +C+C L WL EW R + G+ P CQ P LK P+ ++ + F C+ D C+ + +CP++C C T + CS LK +P IP + TELYLD N TL N +L + LS N I+ ++ + + L TL+LS+N+L+CI F L +L++L L ND+S +P AF DL+ L+++A+G NP +CDCN++WL+ + + + GI+ CA P M K + + +F C D I AKC+ C+ PC+N+G C+ Y C CP F G+ C+ I+AC PC++GG C K + G + C+C GF G C N+DDC + CEN +TCVD +N Y+C CP + G+ CE K +C D NPC++ C L+ +KC CT G+ G++C+ + DDC+ N CKN A C D VNGY CIC GY G +CE F+ + S C +C N C+ + + C+C PGY G C+ L S+ F K+S+++I P ++ + P+ NI+L+ T+ SG++LY+G DK ++ V L+RG ++ SY+ G +S++ +ND H + + + ++L++ ++G + + L + D+ +++GG P K +++ N + F GCI +L IN + DF K + + PGC P +C+ +G C ++ + C C+ G++G C +R T + + H CL I F Y
Sbjct: 26 QACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV----GLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCSVLHCPAACTCSNN-IVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIE-TSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGN---PRCQKPYFLKEIPIQDVAIQDFTCDDGND-------------------DNSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQF-TLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPV-DVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEEDG-FWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKC-ILTPKGFKCDCTPGYVGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCICPEGYSGLFCE--------------------------FSPPMVLPRTSPCDNFDCQNGAQCI---VRINEPICQCLPGYQGEKCEKLVSVNFINKESYLQI-PSAK--VRPQTNITLQIATDEDSGILLYKG-DKDHIAVELYRGRVRASYDTGSHPASAI-YSVETINDGNFHIVELLALDQSLSLSVDGGNPKIITN--LSKQSTLNFDSPLYVGGMPGKSNVASLRQAPGQNGTSFHGCIRNLYINSELQDFQK-VPMQTGILPGCE------------------------------PCHKKVCA-------HGTCQPSSQAG--FTCECQEGWMGPLCDQR-------TNDPCLGNKCVHGTCLPINAFSY 1393 HSP 2 Score: 702.205 bits (1811), Expect = 0.000e+0 Identity = 471/1483 (31.76%), Postives = 734/1483 (49.49%), Query Frame = -2
Query: 5253 EICPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXIQHLPDHLFSKLSKLQKLDLRENQLQ------------------------CLNEHVFSKLGMLKTLLLEGNKLYWISEA-ITKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCANEENTCQPDRKV--CPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXLTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNS-ESDIKYDTHD------ENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNEL------------------------HCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGIC--KNTDNFGNYECVCPSGFRGPKCTENIDDCGGHKCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYC-HDKNPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNSLYF-GKDSHIEIHPPSRGFLMPKGNISLKFITNSSSGLILYQGFDKSYLVVNLFRGHIKISYNIGDKDIGPDGFSMDVVNDNKLHYLNIEIVGENLTMYLNGKVHFTLKSKYLMTNQYMALDTGIFIGGAPKKILNTAIQNHHFDNDSDFTGCIESLLINRKKIDFSKYMSKSVNVKPGCGDLISKTAQELPSSEVPTQDIEVPIKSTKSLPVKSTICSKPGVCSNNGVCHEVNEKNRKYKCVCKNGFVGRKCKKRVSKCHGKTTRTFIKDPDTHDNCLSIKKFQY 9515
+ CP C C +V+C R+VP + T RL+L GN I I K+DF L +L+VLQL N+IS+IE GAF +L L +LRLNRN +Q P+ LF +KL +LDL ENQ+Q C+ + F L L+ L L N + +S A + LR R+ SN L+C+CHL WL+ +L+ + + C+ P L ++ + EF C+ ++ P V CP CTCS + IV+C GK LT IP +LP E+ L+ N+I ++ G S YK L++I L+ N + ++ P AF GL+ L +L L N + + ++ F GL SL L L+ N+I CL F+ + L LL L +NKL + + PL ++ ++L NP CDC + WL L + T A C P + +++ F+C++ E T D +C + CP KC C V+CS L+ +PE I T L L++N+ L+ + L K++ SNN+I +I+ F ++ + EI L+SN+L+ V ++ FK L L+ L L N I+C+G +SF ++++ L L +N++ +C+C L WL EW R + G+ P CQ P LK P+ ++ + F C+ D C+ + +CP++C C T + CS LK +P IP + TELYLD N TL N +L + LS N I+ ++ + + L TL+LS+N+L+CI F L +L++L L ND+S +P AF DL+ L+++A+G NP +CDCN++WL+ + + + GI+ CA P M K + + +F C D I AKC+ C+ PC+N+G C+ Y C CP F G+ C+ I+AC PC++GG C K + G + C+C GF G C N+DDC + CEN +TCVD +N Y+C CP + G+ CE K +C D NPC++ C L+ +KC CT G+ G++C+ + DDC+ N CKN A C D VNGY CIC GY G +CE F+ + S C +C N C+ + + C+C PGY G C+ L S+ F K+S+++I P ++ + P+ NI+L+ T+ SG++LY+G DK ++ V L+RG ++ SY+ G +S++ +ND H + + + ++L++ ++G + + L + D+ +++GG P K +++ N + F GCI +L IN + DF K + + PGC P +C+ +G C ++ + C C+ G++G C +R T + + H CL I F Y
Sbjct: 26 QACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV----GLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCSVLHCPAACTCSNN-IVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIE-TSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGN---PRCQKPYFLKEIPIQDVAIQDFTCDDGND-------------------DNSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQF-TLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPV-DVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEEDG-FWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKC-ILTPKGFKCDCTPGYVGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCICPEGYSGLFCE--------------------------FSPPMVLPRTSPCDNFDCQNGAQCI---VRINEPICQCLPGYQGEKCEKLVSVNFINKESYLQI-PSAK--VRPQTNITLQIATDEDSGILLYKG-DKDHIAVELYRGRVRASYDTGSHPASAI-YSVETINDGNFHIVELLALDQSLSLSVDGGNPKIITN--LSKQSTLNFDSPLYVGGMPGKSNVASLRQAPGQNGTSFHGCIRNLYINSELQDFQK-VPMQTGILPGCE------------------------------PCHKKVCA-------HGTCQPSSQAG--FTCECQEGWMGPLCDQR-------TNDPCLGNKCVHGTCLPINAFSY 1393 HSP 3 Score: 291.582 bits (745), Expect = 2.393e-78 Identity = 222/701 (31.67%), Postives = 332/701 (47.36%), Query Frame = 2
Query: 101 PSEEVEICPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXXQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISEA-ITKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCANEENTCQPDRK--------------VCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQ-GFXXXXXXXXXXXXTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKAN--FSYSCCLDMKCNKIPKCPSKCKC 2149
PS V CP C C N V+C+ + +P L E T + L N I VI F L+ + LSNN+IS + AF L +L L L N I LP LF L LQ L L N++ CL F L L L L NKL I++ + L+ ++ + ++ N C+CHL WL +L +T C +P+RL+ I + + +F+C+ +E P + CP++C C + V+C+ ++L IP+ +P T EL L NN T+L G K L+KI + N + DIE GAF G + + L N L ++ F GL SL L L +N I C+ +F G+ + LL L +N+++ + L L L L+ NP C+C + WL + L+ K + T C P I ++ + +F C+ +D D +C P CP +C+C +V CS GL LP+ I T L L N + + S ++ +L +DLSNN+I + +FS T+L + LS N+L+C+ TF L L +L L+ N IS + +F + L +L + N L+CDC ++WLS+W ++ Y G R C P + L K F C+G + + AK N S C D CN P +C C
Sbjct: 266 PSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNP----IETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRC-EGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRI-VTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGND------DNSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQFTLVPKELSNYK--HLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIAR---CAGPGEMADKLLLTTPSKKFTCQG----PVDVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTC 945 HSP 4 Score: 291.582 bits (745), Expect = 2.393e-78 Identity = 222/701 (31.67%), Postives = 332/701 (47.36%), Query Frame = -2
Query: 7482 PSEEVEICPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXIQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISEA-ITKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCANEENTCQPDRK--------------VCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQ-GFXXXXXXXXXXXLTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKAN--FSYSCCLDMKCNKIPKCPSKCKC 9530
PS V CP C C N V+C+ + +P L E T + L N I VI F L+ + LSNN+IS + AF L +L L L N I LP LF L LQ L L N++ CL F L L L L NKL I++ + L+ ++ + ++ N C+CHL WL +L +T C +P+RL+ I + + +F+C+ +E P + CP++C C + V+C+ ++L IP+ +P T EL L NN T+L G K L+KI + N + DIE GAF G + + L N L ++ F GL SL L L +N I C+ +F G+ + LL L +N+++ + L L L L+ NP C+C + WL + L+ K + T C P I ++ + +F C+ +D D +C P CP +C+C +V CS GL LP+ I T L L N + + S ++ +L +DLSNN+I + +FS T+L + LS N+L+C+ TF L L +L L+ N IS + +F + L +L + N L+CDC ++WLS+W ++ Y G R C P + L K F C+G + + AK N S C D CN P +C C
Sbjct: 266 PSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNP----IETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRC-EGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRI-VTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGND------DNSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQFTLVPKELSNYK--HLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIAR---CAGPGEMADKLLLTTPSKKFTCQG----PVDVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTC 945 HSP 5 Score: 207.223 bits (526), Expect = 2.587e-52 Identity = 137/484 (28.31%), Postives = 217/484 (44.83%), Query Frame = 2
Query: 1451 NCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNL------------------DQIYSVHQA-----------------------------SNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVL------------------------SFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETE 2689
N V CP +CSCS V+C G+ L S+P I +T L L+ N+I + ++I ++ + + L +LDLS NQI+ I F + +K + L N++ C+ + F+ L +LE+L LN+N+I+ + SF L+ L++N L+CDC L WLS+W R + C P L+G + + ++ FVC G++S + S + CP+ C C + +DC L EIPT +P TE+ L++N +K + +F L+ + LS N I+E+ D L++L +LVL + NK+ C+ DAF+DL NL +L L N + TI F L + + L QNPF CDC++KWL + ++ + C SP R+ K I K +F CS + +
Sbjct: 21 NKVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCS------------------VLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQ 483 HSP 6 Score: 207.223 bits (526), Expect = 2.587e-52 Identity = 137/484 (28.31%), Postives = 217/484 (44.83%), Query Frame = -2
Query: 6942 NCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNL------------------DQIYSVHQA-----------------------------SNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVL------------------------SFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETE 8180
N V CP +CSCS V+C G+ L S+P I +T L L+ N+I + ++I ++ + + L +LDLS NQI+ I F + +K + L N++ C+ + F+ L +LE+L LN+N+I+ + SF L+ L++N L+CDC L WLS+W R + C P L+G + + ++ FVC G++S + S + CP+ C C + +DC L EIPT +P TE+ L++N +K + +F L+ + LS N I+E+ D L++L +LVL + NK+ C+ DAF+DL NL +L L N + TI F L + + L QNPF CDC++KWL + ++ + C SP R+ K I K +F CS + +
Sbjct: 21 NKVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCS------------------VLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQ 483
BLAST of slit vs. RefSeq Drosophila melanogaster
Match: gi|17136484|ref|NP_476729.1| (slit, isoform B [Drosophila melanogaster]) HSP 1 Score: 758.829 bits (1958), Expect = 0.000e+0 Identity = 495/1514 (32.69%), Postives = 761/1514 (50.26%), Query Frame = 2
Query: 83 TIWINDPSEEVEI-----CPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXXQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISEAI-TKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCANEENTCQPD---RKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXXTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGH-KCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDK-NPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNSLYF-GKDSHIEIHPPSRGFLMPKGNISLKFITNSSSGLILYQGFDKSYLVVNLFRGHIKISYNIGDKDIGPDGFSMDVVNDNKLHYLNIEIVGENLTMYLNGKVHFTLKSKYLMTNQYMALDTGIFIGGAPKKILNTAIQNHHFDNDSDFTGCIESLLINRKKIDFSKYMSKSVNVKPGCGDLISKTAQELPSSEVPTQDIEVPIKSTKSLPVKSTICSKPGVCSNNGVCHEVNEKNRKYKCVCKNGFVGRKCKKRVSKCHGKTTRTFIKDPDTHDNCLSIKKFQYRVCKGTCSNNRMQADSNRNFYQRSTDNSLSPCCQPSKYTTHKIQFKCDNGRFYRRSVTLPRSCKCQGNCH 4588
++ I+ P V + CP+ C C +V+C HR +VP ++ RL L GN + VI ++DF +L L++LQL++N+I +IE +F +L++L +L ++ N+I + +F L+ L L NQ+ CL+EH F L L+ L L N L + I L LR LR+S N C+CHL WL+ FL+ + + P TR C +P +L ++ L EF+C+ + CP C C+ DGIV+C K LT++P LP +T EL L+ N IT L S ++ L++I L+ N + I A GL+QLT L L N ++ + F GL SL L L+ NEI C+ F + L LL L +N + +L + ++ ++L NP CDC + WL L + T A C P H ++SL+ F+C S +++ E C CP C C V+C+G GL +P I TT L+L+ N++ + + +LVKL+L NQ+ I+P+ F ++ ++E+ L NK+K ++N+ F L +L+ L L DN ISC+ SF+ +L L L +N +C+C L W +EW R G+ R C +P ++ + +L F C S E + C + CP C C T + CS QLKEIP IP T+ELYL+ N+++ ++ ++L +L L LS N IT ++ L L TL++S+NKLQC+ + A L+NL+VL L N +S +P +F DL +L +IALG NP +CDC +KW + + +++ GI+ CA PE+MK K I + + F+C + I AKC+AC + PCQN +C + +Y C C +HGK CE I+AC+G PCRN C + G + C C G+ G +C NIDDC G KC+N ATC+D V Y C+C GF G+FC++K +C + NPC N C + Y C C G+ G NC NIDDC+ ++C+N C D +N Y+C C Y G YCE + +S + S C HEC +GVC + C+C PGY+G +C+YL S+ F +S +E+ P P+ N+++ F + +G+++Y G D ++L V LF G I++SY++G+ + +S ++V D K H + + + +N T+ ++ + ++ ++ +N Y+ L T +F+GG P A +N N + F GC++ + IN K +DF + + PGC L+ QE E D IK P C + C N + Y+C CK+G GR C + S C + R + T ++C S + +Y C G C N CC K++ C N R Y +++ + R C C C+
Sbjct: 55 SVGIHIPGGGVGVITEARCPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRL-APYTR---CQSPSQLKGQNVADLHDQEFKCSGLTEHAPMECGAENSCPHPCRCA-DGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIE-TSGARCESPKRMHRRRIESLREEKFKC-SWDELRMKLSGE-CRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAAR---CGAPSKVRDVQIKDLPHSEFKC-----SSENSEGCLGDGY---------------CPPSCTCTGTVVRCSRNQLKEIPRGIPAETSELYLESNEIEQIHYERIRHLRSLTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCGLKWFSDWIKLDYVEPGIARCAEPEQMKDKLILSTPSSSFVCRGRVRND-ILAKCNACFEQPCQNQAQCVALPQREYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEE-GRFSCQCAPGYTGARCETNIDDCLGEIKCQNNATCIDGVESYKCECQPGFSGEFCDTKIQFCSPEFNPCANGAKCMDHFTH-YSCDCQAGFHGTNCTDNIDDCQNHMCQNGGTCVDGINDYQCRCPDDYTGKYCE------------------GHNMISMMYPQT------SPCQNHEC-KHGVCFQPNAQGSDYLCRCHPGYTGKWCEYLTSISFVHNNSFVELEPLR---TRPEANVTIVFSSAEQNGILMYDGQD-AHLAVELFNGRIRVSYDVGNHPVS-TMYSFEMVADGKYHAVELLAIKKNFTLRVDRGLARSIINE--GSNDYLKLTTPMFLGGLPVDPAQQAYKNWQIRNLTSFKGCMKEVWINHKLVDFGN-AQRQQKITPGCA-LLEGEQQEEEDDEQDFMDETPHIKEEPVDPCLENKCRRGSRCVPNSNARD------GYQCKCKHGQRGRYCDQAASTCRKEQVREYY----TENDCRSRQPLKYAKCVGGCGNQ---------------------CCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKKCY 1469 HSP 2 Score: 758.829 bits (1958), Expect = 0.000e+0 Identity = 495/1514 (32.69%), Postives = 761/1514 (50.26%), Query Frame = -2
Query: 5043 TIWINDPSEEVEI-----CPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXIQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISEAI-TKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCANEENTCQPD---RKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXLTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGH-KCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDK-NPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNSLYF-GKDSHIEIHPPSRGFLMPKGNISLKFITNSSSGLILYQGFDKSYLVVNLFRGHIKISYNIGDKDIGPDGFSMDVVNDNKLHYLNIEIVGENLTMYLNGKVHFTLKSKYLMTNQYMALDTGIFIGGAPKKILNTAIQNHHFDNDSDFTGCIESLLINRKKIDFSKYMSKSVNVKPGCGDLISKTAQELPSSEVPTQDIEVPIKSTKSLPVKSTICSKPGVCSNNGVCHEVNEKNRKYKCVCKNGFVGRKCKKRVSKCHGKTTRTFIKDPDTHDNCLSIKKFQYRVCKGTCSNNRMQADSNRNFYQRSTDNSLSPCCQPSKYTTHKIQFKCDNGRFYRRSVTLPRSCKCQGNCH 9548
++ I+ P V + CP+ C C +V+C HR +VP ++ RL L GN + VI ++DF +L L++LQL++N+I +IE +F +L++L +L ++ N+I + +F L+ L L NQ+ CL+EH F L L+ L L N L + I L LR LR+S N C+CHL WL+ FL+ + + P TR C +P +L ++ L EF+C+ + CP C C+ DGIV+C K LT++P LP +T EL L+ N IT L S ++ L++I L+ N + I A GL+QLT L L N ++ + F GL SL L L+ NEI C+ F + L LL L +N + +L + ++ ++L NP CDC + WL L + T A C P H ++SL+ F+C S +++ E C CP C C V+C+G GL +P I TT L+L+ N++ + + +LVKL+L NQ+ I+P+ F ++ ++E+ L NK+K ++N+ F L +L+ L L DN ISC+ SF+ +L L L +N +C+C L W +EW R G+ R C +P ++ + +L F C S E + C + CP C C T + CS QLKEIP IP T+ELYL+ N+++ ++ ++L +L L LS N IT ++ L L TL++S+NKLQC+ + A L+NL+VL L N +S +P +F DL +L +IALG NP +CDC +KW + + +++ GI+ CA PE+MK K I + + F+C + I AKC+AC + PCQN +C + +Y C C +HGK CE I+AC+G PCRN C + G + C C G+ G +C NIDDC G KC+N ATC+D V Y C+C GF G+FC++K +C + NPC N C + Y C C G+ G NC NIDDC+ ++C+N C D +N Y+C C Y G YCE + +S + S C HEC +GVC + C+C PGY+G +C+YL S+ F +S +E+ P P+ N+++ F + +G+++Y G D ++L V LF G I++SY++G+ + +S ++V D K H + + + +N T+ ++ + ++ ++ +N Y+ L T +F+GG P A +N N + F GC++ + IN K +DF + + PGC L+ QE E D IK P C + C N + Y+C CK+G GR C + S C + R + T ++C S + +Y C G C N CC K++ C N R Y +++ + R C C C+
Sbjct: 55 SVGIHIPGGGVGVITEARCPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRL-APYTR---CQSPSQLKGQNVADLHDQEFKCSGLTEHAPMECGAENSCPHPCRCA-DGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIE-TSGARCESPKRMHRRRIESLREEKFKC-SWDELRMKLSGE-CRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAAR---CGAPSKVRDVQIKDLPHSEFKC-----SSENSEGCLGDGY---------------CPPSCTCTGTVVRCSRNQLKEIPRGIPAETSELYLESNEIEQIHYERIRHLRSLTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCGLKWFSDWIKLDYVEPGIARCAEPEQMKDKLILSTPSSSFVCRGRVRND-ILAKCNACFEQPCQNQAQCVALPQREYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEE-GRFSCQCAPGYTGARCETNIDDCLGEIKCQNNATCIDGVESYKCECQPGFSGEFCDTKIQFCSPEFNPCANGAKCMDHFTH-YSCDCQAGFHGTNCTDNIDDCQNHMCQNGGTCVDGINDYQCRCPDDYTGKYCE------------------GHNMISMMYPQT------SPCQNHEC-KHGVCFQPNAQGSDYLCRCHPGYTGKWCEYLTSISFVHNNSFVELEPLR---TRPEANVTIVFSSAEQNGILMYDGQD-AHLAVELFNGRIRVSYDVGNHPVS-TMYSFEMVADGKYHAVELLAIKKNFTLRVDRGLARSIINE--GSNDYLKLTTPMFLGGLPVDPAQQAYKNWQIRNLTSFKGCMKEVWINHKLVDFGN-AQRQQKITPGCA-LLEGEQQEEEDDEQDFMDETPHIKEEPVDPCLENKCRRGSRCVPNSNARD------GYQCKCKHGQRGRYCDQAASTCRKEQVREYY----TENDCRSRQPLKYAKCVGGCGNQ---------------------CCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKKCY 1469
BLAST of slit vs. RefSeq Drosophila melanogaster
Match: gi|17136482|ref|NP_476728.1| (slit, isoform A [Drosophila melanogaster]) HSP 1 Score: 750.74 bits (1937), Expect = 0.000e+0 Identity = 495/1525 (32.46%), Postives = 761/1525 (49.90%), Query Frame = 2
Query: 83 TIWINDPSEEVEI-----CPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXXQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISEAI-TKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCANEENTCQPD---RKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXXTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGH-KCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDK-NPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNSLYF-GKDSHIEIHPPSRGFLMPKGNISLKFITNSSSGLILYQGFDKSYLVVNLFRGHIKISYNIGDKDIGPDGFSMDVVNDNKLHYLNIEIVGENLTMYLNGKVHFTLKSKYLMTNQYMALDTGIFIGGAPKKILNTAIQNHHFDNDSDFTGCIESLLINRKKIDFSKYMSKSVNVKPGCGDLISKTAQELPSSEVPTQDIEVPIKSTKSLPVKSTICSKPGVCSNNGVCHEVNEKNRKYKCVCKNGFVGRKCKK-----------RVSKCHGKTTRTFIKDPDTHDNCLSIKKFQYRVCKGTCSNNRMQADSNRNFYQRSTDNSLSPCCQPSKYTTHKIQFKCDNGRFYRRSVTLPRSCKCQGNCH 4588
++ I+ P V + CP+ C C +V+C HR +VP ++ RL L GN + VI ++DF +L L++LQL++N+I +IE +F +L++L +L ++ N+I + +F L+ L L NQ+ CL+EH F L L+ L L N L + I L LR LR+S N C+CHL WL+ FL+ + + P TR C +P +L ++ L EF+C+ + CP C C+ DGIV+C K LT++P LP +T EL L+ N IT L S ++ L++I L+ N + I A GL+QLT L L N ++ + F GL SL L L+ NEI C+ F + L LL L +N + +L + ++ ++L NP CDC + WL L + T A C P H ++SL+ F+C S +++ E C CP C C V+C+G GL +P I TT L+L+ N++ + + +LVKL+L NQ+ I+P+ F ++ ++E+ L NK+K ++N+ F L +L+ L L DN ISC+ SF+ +L L L +N +C+C L W +EW R G+ R C +P ++ + +L F C S E + C + CP C C T + CS QLKEIP IP T+ELYL+ N+++ ++ ++L +L L LS N IT ++ L L TL++S+NKLQC+ + A L+NL+VL L N +S +P +F DL +L +IALG NP +CDC +KW + + +++ GI+ CA PE+MK K I + + F+C + I AKC+AC + PCQN +C + +Y C C +HGK CE I+AC+G PCRN C + G + C C G+ G +C NIDDC G KC+N ATC+D V Y C+C GF G+FC++K +C + NPC N C + Y C C G+ G NC NIDDC+ ++C+N C D +N Y+C C Y G YCE + +S + S C HEC +GVC + C+C PGY+G +C+YL S+ F +S +E+ P P+ N+++ F + +G+++Y G D ++L V LF G I++SY++G+ + +S ++V D K H + + + +N T+ ++ + ++ ++ +N Y+ L T +F+GG P A +N N + F GC++ + IN K +DF + + PGC L+ QE E D IK P C + C N + Y+C CK+G GR C + S C + R + T ++C S + +Y C G C N CC K++ C N R Y +++ + R C C C+
Sbjct: 55 SVGIHIPGGGVGVITEARCPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRL-APYTR---CQSPSQLKGQNVADLHDQEFKCSGLTEHAPMECGAENSCPHPCRCA-DGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIE-TSGARCESPKRMHRRRIESLREEKFKC-SWDELRMKLSGE-CRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAAR---CGAPSKVRDVQIKDLPHSEFKC-----SSENSEGCLGDGY---------------CPPSCTCTGTVVRCSRNQLKEIPRGIPAETSELYLESNEIEQIHYERIRHLRSLTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCGLKWFSDWIKLDYVEPGIARCAEPEQMKDKLILSTPSSSFVCRGRVRND-ILAKCNACFEQPCQNQAQCVALPQREYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEE-GRFSCQCAPGYTGARCETNIDDCLGEIKCQNNATCIDGVESYKCECQPGFSGEFCDTKIQFCSPEFNPCANGAKCMDHFTH-YSCDCQAGFHGTNCTDNIDDCQNHMCQNGGTCVDGINDYQCRCPDDYTGKYCE------------------GHNMISMMYPQT------SPCQNHEC-KHGVCFQPNAQGSDYLCRCHPGYTGKWCEYLTSISFVHNNSFVELEPLR---TRPEANVTIVFSSAEQNGILMYDGQD-AHLAVELFNGRIRVSYDVGNHPVS-TMYSFEMVADGKYHAVELLAIKKNFTLRVDRGLARSIINE--GSNDYLKLTTPMFLGGLPVDPAQQAYKNWQIRNLTSFKGCMKEVWINHKLVDFGN-AQRQQKITPGCA-LLEGEQQEEEDDEQDFMDETPHIKEEPVDPCLENKCRRGSRCVPNSNARD------GYQCKCKHGQRGRYCDQGEGSTEPPTVTAASTCRKEQVREYY----TENDCRSRQPLKYAKCVGGCGNQ---------------------CCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKKCY 1480 HSP 2 Score: 750.74 bits (1937), Expect = 0.000e+0 Identity = 495/1525 (32.46%), Postives = 761/1525 (49.90%), Query Frame = -2
Query: 5043 TIWINDPSEEVEI-----CPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXIQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISEAI-TKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCANEENTCQPD---RKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXLTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGH-KCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDK-NPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNSLYF-GKDSHIEIHPPSRGFLMPKGNISLKFITNSSSGLILYQGFDKSYLVVNLFRGHIKISYNIGDKDIGPDGFSMDVVNDNKLHYLNIEIVGENLTMYLNGKVHFTLKSKYLMTNQYMALDTGIFIGGAPKKILNTAIQNHHFDNDSDFTGCIESLLINRKKIDFSKYMSKSVNVKPGCGDLISKTAQELPSSEVPTQDIEVPIKSTKSLPVKSTICSKPGVCSNNGVCHEVNEKNRKYKCVCKNGFVGRKCKK-----------RVSKCHGKTTRTFIKDPDTHDNCLSIKKFQYRVCKGTCSNNRMQADSNRNFYQRSTDNSLSPCCQPSKYTTHKIQFKCDNGRFYRRSVTLPRSCKCQGNCH 9548
++ I+ P V + CP+ C C +V+C HR +VP ++ RL L GN + VI ++DF +L L++LQL++N+I +IE +F +L++L +L ++ N+I + +F L+ L L NQ+ CL+EH F L L+ L L N L + I L LR LR+S N C+CHL WL+ FL+ + + P TR C +P +L ++ L EF+C+ + CP C C+ DGIV+C K LT++P LP +T EL L+ N IT L S ++ L++I L+ N + I A GL+QLT L L N ++ + F GL SL L L+ NEI C+ F + L LL L +N + +L + ++ ++L NP CDC + WL L + T A C P H ++SL+ F+C S +++ E C CP C C V+C+G GL +P I TT L+L+ N++ + + +LVKL+L NQ+ I+P+ F ++ ++E+ L NK+K ++N+ F L +L+ L L DN ISC+ SF+ +L L L +N +C+C L W +EW R G+ R C +P ++ + +L F C S E + C + CP C C T + CS QLKEIP IP T+ELYL+ N+++ ++ ++L +L L LS N IT ++ L L TL++S+NKLQC+ + A L+NL+VL L N +S +P +F DL +L +IALG NP +CDC +KW + + +++ GI+ CA PE+MK K I + + F+C + I AKC+AC + PCQN +C + +Y C C +HGK CE I+AC+G PCRN C + G + C C G+ G +C NIDDC G KC+N ATC+D V Y C+C GF G+FC++K +C + NPC N C + Y C C G+ G NC NIDDC+ ++C+N C D +N Y+C C Y G YCE + +S + S C HEC +GVC + C+C PGY+G +C+YL S+ F +S +E+ P P+ N+++ F + +G+++Y G D ++L V LF G I++SY++G+ + +S ++V D K H + + + +N T+ ++ + ++ ++ +N Y+ L T +F+GG P A +N N + F GC++ + IN K +DF + + PGC L+ QE E D IK P C + C N + Y+C CK+G GR C + S C + R + T ++C S + +Y C G C N CC K++ C N R Y +++ + R C C C+
Sbjct: 55 SVGIHIPGGGVGVITEARCPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRL-APYTR---CQSPSQLKGQNVADLHDQEFKCSGLTEHAPMECGAENSCPHPCRCA-DGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIE-TSGARCESPKRMHRRRIESLREEKFKC-SWDELRMKLSGE-CRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAAR---CGAPSKVRDVQIKDLPHSEFKC-----SSENSEGCLGDGY---------------CPPSCTCTGTVVRCSRNQLKEIPRGIPAETSELYLESNEIEQIHYERIRHLRSLTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCGLKWFSDWIKLDYVEPGIARCAEPEQMKDKLILSTPSSSFVCRGRVRND-ILAKCNACFEQPCQNQAQCVALPQREYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEE-GRFSCQCAPGYTGARCETNIDDCLGEIKCQNNATCIDGVESYKCECQPGFSGEFCDTKIQFCSPEFNPCANGAKCMDHFTH-YSCDCQAGFHGTNCTDNIDDCQNHMCQNGGTCVDGINDYQCRCPDDYTGKYCE------------------GHNMISMMYPQT------SPCQNHEC-KHGVCFQPNAQGSDYLCRCHPGYTGKWCEYLTSISFVHNNSFVELEPLR---TRPEANVTIVFSSAEQNGILMYDGQD-AHLAVELFNGRIRVSYDVGNHPVS-TMYSFEMVADGKYHAVELLAIKKNFTLRVDRGLARSIINE--GSNDYLKLTTPMFLGGLPVDPAQQAYKNWQIRNLTSFKGCMKEVWINHKLVDFGN-AQRQQKITPGCA-LLEGEQQEEEDDEQDFMDETPHIKEEPVDPCLENKCRRGSRCVPNSNARD------GYQCKCKHGQRGRYCDQGEGSTEPPTVTAASTCRKEQVREYY----TENDCRSRQPLKYAKCVGGCGNQ---------------------CCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKKCY 1480
BLAST of slit vs. RefSeq Drosophila melanogaster
Match: gi|161077130|ref|NP_001097333.1| (slit, isoform D [Drosophila melanogaster]) HSP 1 Score: 750.74 bits (1937), Expect = 0.000e+0 Identity = 495/1525 (32.46%), Postives = 761/1525 (49.90%), Query Frame = 2
Query: 83 TIWINDPSEEVEI-----CPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXXQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISEAI-TKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCANEENTCQPD---RKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXXTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGH-KCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDK-NPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNSLYF-GKDSHIEIHPPSRGFLMPKGNISLKFITNSSSGLILYQGFDKSYLVVNLFRGHIKISYNIGDKDIGPDGFSMDVVNDNKLHYLNIEIVGENLTMYLNGKVHFTLKSKYLMTNQYMALDTGIFIGGAPKKILNTAIQNHHFDNDSDFTGCIESLLINRKKIDFSKYMSKSVNVKPGCGDLISKTAQELPSSEVPTQDIEVPIKSTKSLPVKSTICSKPGVCSNNGVCHEVNEKNRKYKCVCKNGFVGRKCKK-----------RVSKCHGKTTRTFIKDPDTHDNCLSIKKFQYRVCKGTCSNNRMQADSNRNFYQRSTDNSLSPCCQPSKYTTHKIQFKCDNGRFYRRSVTLPRSCKCQGNCH 4588
++ I+ P V + CP+ C C +V+C HR +VP ++ RL L GN + VI ++DF +L L++LQL++N+I +IE +F +L++L +L ++ N+I + +F L+ L L NQ+ CL+EH F L L+ L L N L + I L LR LR+S N C+CHL WL+ FL+ + + P TR C +P +L ++ L EF+C+ + CP C C+ DGIV+C K LT++P LP +T EL L+ N IT L S ++ L++I L+ N + I A GL+QLT L L N ++ + F GL SL L L+ NEI C+ F + L LL L +N + +L + ++ ++L NP CDC + WL L + T A C P H ++SL+ F+C S +++ E C CP C C V+C+G GL +P I TT L+L+ N++ + + +LVKL+L NQ+ I+P+ F ++ ++E+ L NK+K ++N+ F L +L+ L L DN ISC+ SF+ +L L L +N +C+C L W +EW R G+ R C +P ++ + +L F C S E + C + CP C C T + CS QLKEIP IP T+ELYL+ N+++ ++ ++L +L L LS N IT ++ L L TL++S+NKLQC+ + A L+NL+VL L N +S +P +F DL +L +IALG NP +CDC +KW + + +++ GI+ CA PE+MK K I + + F+C + I AKC+AC + PCQN +C + +Y C C +HGK CE I+AC+G PCRN C + G + C C G+ G +C NIDDC G KC+N ATC+D V Y C+C GF G+FC++K +C + NPC N C + Y C C G+ G NC NIDDC+ ++C+N C D +N Y+C C Y G YCE + +S + S C HEC +GVC + C+C PGY+G +C+YL S+ F +S +E+ P P+ N+++ F + +G+++Y G D ++L V LF G I++SY++G+ + +S ++V D K H + + + +N T+ ++ + ++ ++ +N Y+ L T +F+GG P A +N N + F GC++ + IN K +DF + + PGC L+ QE E D IK P C + C N + Y+C CK+G GR C + S C + R + T ++C S + +Y C G C N CC K++ C N R Y +++ + R C C C+
Sbjct: 55 SVGIHIPGGGVGVITEARCPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRL-APYTR---CQSPSQLKGQNVADLHDQEFKCSGLTEHAPMECGAENSCPHPCRCA-DGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIE-TSGARCESPKRMHRRRIESLREEKFKC-SWDELRMKLSGE-CRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAAR---CGAPSKVRDVQIKDLPHSEFKC-----SSENSEGCLGDGY---------------CPPSCTCTGTVVRCSRNQLKEIPRGIPAETSELYLESNEIEQIHYERIRHLRSLTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCGLKWFSDWIKLDYVEPGIARCAEPEQMKDKLILSTPSSSFVCRGRVRND-ILAKCNACFEQPCQNQAQCVALPQREYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEE-GRFSCQCAPGYTGARCETNIDDCLGEIKCQNNATCIDGVESYKCECQPGFSGEFCDTKIQFCSPEFNPCANGAKCMDHFTH-YSCDCQAGFHGTNCTDNIDDCQNHMCQNGGTCVDGINDYQCRCPDDYTGKYCE------------------GHNMISMMYPQT------SPCQNHEC-KHGVCFQPNAQGSDYLCRCHPGYTGKWCEYLTSISFVHNNSFVELEPLR---TRPEANVTIVFSSAEQNGILMYDGQD-AHLAVELFNGRIRVSYDVGNHPVS-TMYSFEMVADGKYHAVELLAIKKNFTLRVDRGLARSIINE--GSNDYLKLTTPMFLGGLPVDPAQQAYKNWQIRNLTSFKGCMKEVWINHKLVDFGN-AQRQQKITPGCA-LLEGEQQEEEDDEQDFMDETPHIKEEPVDPCLENKCRRGSRCVPNSNARD------GYQCKCKHGQRGRYCDQGEGSTEPPTVTAASTCRKEQVREYY----TENDCRSRQPLKYAKCVGGCGNQ---------------------CCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKKCY 1480 HSP 2 Score: 750.74 bits (1937), Expect = 0.000e+0 Identity = 495/1525 (32.46%), Postives = 761/1525 (49.90%), Query Frame = -2
Query: 5043 TIWINDPSEEVEI-----CPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXIQHLPDHLFSKLSKLQKLDLRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISEAI-TKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCANEENTCQPD---RKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXLTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGH-KCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDK-NPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNSLYF-GKDSHIEIHPPSRGFLMPKGNISLKFITNSSSGLILYQGFDKSYLVVNLFRGHIKISYNIGDKDIGPDGFSMDVVNDNKLHYLNIEIVGENLTMYLNGKVHFTLKSKYLMTNQYMALDTGIFIGGAPKKILNTAIQNHHFDNDSDFTGCIESLLINRKKIDFSKYMSKSVNVKPGCGDLISKTAQELPSSEVPTQDIEVPIKSTKSLPVKSTICSKPGVCSNNGVCHEVNEKNRKYKCVCKNGFVGRKCKK-----------RVSKCHGKTTRTFIKDPDTHDNCLSIKKFQYRVCKGTCSNNRMQADSNRNFYQRSTDNSLSPCCQPSKYTTHKIQFKCDNGRFYRRSVTLPRSCKCQGNCH 9548
++ I+ P V + CP+ C C +V+C HR +VP ++ RL L GN + VI ++DF +L L++LQL++N+I +IE +F +L++L +L ++ N+I + +F L+ L L NQ+ CL+EH F L L+ L L N L + I L LR LR+S N C+CHL WL+ FL+ + + P TR C +P +L ++ L EF+C+ + CP C C+ DGIV+C K LT++P LP +T EL L+ N IT L S ++ L++I L+ N + I A GL+QLT L L N ++ + F GL SL L L+ NEI C+ F + L LL L +N + +L + ++ ++L NP CDC + WL L + T A C P H ++SL+ F+C S +++ E C CP C C V+C+G GL +P I TT L+L+ N++ + + +LVKL+L NQ+ I+P+ F ++ ++E+ L NK+K ++N+ F L +L+ L L DN ISC+ SF+ +L L L +N +C+C L W +EW R G+ R C +P ++ + +L F C S E + C + CP C C T + CS QLKEIP IP T+ELYL+ N+++ ++ ++L +L L LS N IT ++ L L TL++S+NKLQC+ + A L+NL+VL L N +S +P +F DL +L +IALG NP +CDC +KW + + +++ GI+ CA PE+MK K I + + F+C + I AKC+AC + PCQN +C + +Y C C +HGK CE I+AC+G PCRN C + G + C C G+ G +C NIDDC G KC+N ATC+D V Y C+C GF G+FC++K +C + NPC N C + Y C C G+ G NC NIDDC+ ++C+N C D +N Y+C C Y G YCE + +S + S C HEC +GVC + C+C PGY+G +C+YL S+ F +S +E+ P P+ N+++ F + +G+++Y G D ++L V LF G I++SY++G+ + +S ++V D K H + + + +N T+ ++ + ++ ++ +N Y+ L T +F+GG P A +N N + F GC++ + IN K +DF + + PGC L+ QE E D IK P C + C N + Y+C CK+G GR C + S C + R + T ++C S + +Y C G C N CC K++ C N R Y +++ + R C C C+
Sbjct: 55 SVGIHIPGGGVGVITEARCPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRL-APYTR---CQSPSQLKGQNVADLHDQEFKCSGLTEHAPMECGAENSCPHPCRCA-DGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIE-TSGARCESPKRMHRRRIESLREEKFKC-SWDELRMKLSGE-CRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAAR---CGAPSKVRDVQIKDLPHSEFKC-----SSENSEGCLGDGY---------------CPPSCTCTGTVVRCSRNQLKEIPRGIPAETSELYLESNEIEQIHYERIRHLRSLTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCGLKWFSDWIKLDYVEPGIARCAEPEQMKDKLILSTPSSSFVCRGRVRND-ILAKCNACFEQPCQNQAQCVALPQREYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEE-GRFSCQCAPGYTGARCETNIDDCLGEIKCQNNATCIDGVESYKCECQPGFSGEFCDTKIQFCSPEFNPCANGAKCMDHFTH-YSCDCQAGFHGTNCTDNIDDCQNHMCQNGGTCVDGINDYQCRCPDDYTGKYCE------------------GHNMISMMYPQT------SPCQNHEC-KHGVCFQPNAQGSDYLCRCHPGYTGKWCEYLTSISFVHNNSFVELEPLR---TRPEANVTIVFSSAEQNGILMYDGQD-AHLAVELFNGRIRVSYDVGNHPVS-TMYSFEMVADGKYHAVELLAIKKNFTLRVDRGLARSIINE--GSNDYLKLTTPMFLGGLPVDPAQQAYKNWQIRNLTSFKGCMKEVWINHKLVDFGN-AQRQQKITPGCA-LLEGEQQEEEDDEQDFMDETPHIKEEPVDPCLENKCRRGSRCVPNSNARD------GYQCKCKHGQRGRYCDQGEGSTEPPTVTAASTCRKEQVREYY----TENDCRSRQPLKYAKCVGGCGNQ---------------------CCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKKCY 1480
BLAST of slit vs. RefSeq Drosophila melanogaster
Match: gi|17136480|ref|NP_476727.1| (slit, isoform C [Drosophila melanogaster]) HSP 1 Score: 742.265 bits (1915), Expect = 0.000e+0 Identity = 498/1549 (32.15%), Postives = 766/1549 (49.45%), Query Frame = 2
Query: 83 TIWINDPSEEVEI-----CPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXXQHLPDHLFSKLSKLQKLD------------------------LRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISEAI-TKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCANEENTCQPD---RKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXXTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGH-KCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDK-NPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNSLYF-GKDSHIEIHPPSRGFLMPKGNISLKFITNSSSGLILYQGFDKSYLVVNLFRGHIKISYNIGDKDIGPDGFSMDVVNDNKLHYLNIEIVGENLTMYLNGKVHFTLKSKYLMTNQYMALDTGIFIGGAPKKILNTAIQNHHFDNDSDFTGCIESLLINRKKIDFSKYMSKSVNVKPGCGDLISKTAQELPSSEVPTQDIEVPIKSTKSLPVKSTICSKPGVCSNNGVCHEVNEKNRKYKCVCKNGFVGRKCKK-----------RVSKCHGKTTRTFIKDPDTHDNCLSIKKFQYRVCKGTCSNNRMQADSNRNFYQRSTDNSLSPCCQPSKYTTHKIQFKCDNGRFYRRSVTLPRSCKCQGNCH 4588
++ I+ P V + CP+ C C +V+C HR +VP ++ RL L GN + VI ++DF +L L++LQL++N+I +IE +F +L++L +LRLN N ++ +P++ + + L +LD L NQ+ CL+EH F L L+ L L N L + I L LR LR+S N C+CHL WL+ FL+ + + P TR C +P +L ++ L EF+C+ + CP C C+ DGIV+C K LT++P LP +T EL L+ N IT L S ++ L++I L+ N + I A GL+QLT L L N ++ + F GL SL L L+ NEI C+ F + L LL L +N + +L + ++ ++L NP CDC + WL L + T A C P H ++SL+ F+C S +++ E C CP C C V+C+G GL +P I TT L+L+ N++ + + +LVKL+L NQ+ I+P+ F ++ ++E+ L NK+K ++N+ F L +L+ L L DN ISC+ SF+ +L L L +N +C+C L W +EW R G+ R C +P ++ + +L F C S E + C + CP C C T + CS QLKEIP IP T+ELYL+ N+++ ++ ++L +L L LS N IT ++ L L TL++S+NKLQC+ + A L+NL+VL L N +S +P +F DL +L +IALG NP +CDC +KW + + +++ GI+ CA PE+MK K I + + F+C + I AKC+AC + PCQN +C + +Y C C +HGK CE I+AC+G PCRN C + G + C C G+ G +C NIDDC G KC+N ATC+D V Y C+C GF G+FC++K +C + NPC N C + Y C C G+ G NC NIDDC+ ++C+N C D +N Y+C C Y G YCE + +S + S C HEC +GVC + C+C PGY+G +C+YL S+ F +S +E+ P P+ N+++ F + +G+++Y G D ++L V LF G I++SY++G+ + +S ++V D K H + + + +N T+ ++ + ++ ++ +N Y+ L T +F+GG P A +N N + F GC++ + IN K +DF + + PGC L+ QE E D IK P C + C N + Y+C CK+G GR C + S C + R + T ++C S + +Y C G C N CC K++ C N R Y +++ + R C C C+
Sbjct: 55 SVGIHIPGGGVGVITEARCPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLRLNNNRLKAIPENFVTSSASLLRLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRL-APYTR---CQSPSQLKGQNVADLHDQEFKCSGLTEHAPMECGAENSCPHPCRCA-DGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIE-TSGARCESPKRMHRRRIESLREEKFKC-SWDELRMKLSGE-CRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAAR---CGAPSKVRDVQIKDLPHSEFKC-----SSENSEGCLGDGY---------------CPPSCTCTGTVVRCSRNQLKEIPRGIPAETSELYLESNEIEQIHYERIRHLRSLTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCGLKWFSDWIKLDYVEPGIARCAEPEQMKDKLILSTPSSSFVCRGRVRND-ILAKCNACFEQPCQNQAQCVALPQREYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEE-GRFSCQCAPGYTGARCETNIDDCLGEIKCQNNATCIDGVESYKCECQPGFSGEFCDTKIQFCSPEFNPCANGAKCMDHFTH-YSCDCQAGFHGTNCTDNIDDCQNHMCQNGGTCVDGINDYQCRCPDDYTGKYCE------------------GHNMISMMYPQT------SPCQNHEC-KHGVCFQPNAQGSDYLCRCHPGYTGKWCEYLTSISFVHNNSFVELEPLR---TRPEANVTIVFSSAEQNGILMYDGQD-AHLAVELFNGRIRVSYDVGNHPVS-TMYSFEMVADGKYHAVELLAIKKNFTLRVDRGLARSIINE--GSNDYLKLTTPMFLGGLPVDPAQQAYKNWQIRNLTSFKGCMKEVWINHKLVDFGN-AQRQQKITPGCA-LLEGEQQEEEDDEQDFMDETPHIKEEPVDPCLENKCRRGSRCVPNSNARD------GYQCKCKHGQRGRYCDQGEGSTEPPTVTAASTCRKEQVREYY----TENDCRSRQPLKYAKCVGGCGNQ---------------------CCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKKCY 1504 HSP 2 Score: 742.265 bits (1915), Expect = 0.000e+0 Identity = 498/1549 (32.15%), Postives = 766/1549 (49.45%), Query Frame = -2
Query: 5043 TIWINDPSEEVEI-----CPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXIQHLPDHLFSKLSKLQKLD------------------------LRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISEAI-TKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCANEENTCQPD---RKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXLTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGH-KCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDK-NPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNSLYF-GKDSHIEIHPPSRGFLMPKGNISLKFITNSSSGLILYQGFDKSYLVVNLFRGHIKISYNIGDKDIGPDGFSMDVVNDNKLHYLNIEIVGENLTMYLNGKVHFTLKSKYLMTNQYMALDTGIFIGGAPKKILNTAIQNHHFDNDSDFTGCIESLLINRKKIDFSKYMSKSVNVKPGCGDLISKTAQELPSSEVPTQDIEVPIKSTKSLPVKSTICSKPGVCSNNGVCHEVNEKNRKYKCVCKNGFVGRKCKK-----------RVSKCHGKTTRTFIKDPDTHDNCLSIKKFQYRVCKGTCSNNRMQADSNRNFYQRSTDNSLSPCCQPSKYTTHKIQFKCDNGRFYRRSVTLPRSCKCQGNCH 9548
++ I+ P V + CP+ C C +V+C HR +VP ++ RL L GN + VI ++DF +L L++LQL++N+I +IE +F +L++L +LRLN N ++ +P++ + + L +LD L NQ+ CL+EH F L L+ L L N L + I L LR LR+S N C+CHL WL+ FL+ + + P TR C +P +L ++ L EF+C+ + CP C C+ DGIV+C K LT++P LP +T EL L+ N IT L S ++ L++I L+ N + I A GL+QLT L L N ++ + F GL SL L L+ NEI C+ F + L LL L +N + +L + ++ ++L NP CDC + WL L + T A C P H ++SL+ F+C S +++ E C CP C C V+C+G GL +P I TT L+L+ N++ + + +LVKL+L NQ+ I+P+ F ++ ++E+ L NK+K ++N+ F L +L+ L L DN ISC+ SF+ +L L L +N +C+C L W +EW R G+ R C +P ++ + +L F C S E + C + CP C C T + CS QLKEIP IP T+ELYL+ N+++ ++ ++L +L L LS N IT ++ L L TL++S+NKLQC+ + A L+NL+VL L N +S +P +F DL +L +IALG NP +CDC +KW + + +++ GI+ CA PE+MK K I + + F+C + I AKC+AC + PCQN +C + +Y C C +HGK CE I+AC+G PCRN C + G + C C G+ G +C NIDDC G KC+N ATC+D V Y C+C GF G+FC++K +C + NPC N C + Y C C G+ G NC NIDDC+ ++C+N C D +N Y+C C Y G YCE + +S + S C HEC +GVC + C+C PGY+G +C+YL S+ F +S +E+ P P+ N+++ F + +G+++Y G D ++L V LF G I++SY++G+ + +S ++V D K H + + + +N T+ ++ + ++ ++ +N Y+ L T +F+GG P A +N N + F GC++ + IN K +DF + + PGC L+ QE E D IK P C + C N + Y+C CK+G GR C + S C + R + T ++C S + +Y C G C N CC K++ C N R Y +++ + R C C C+
Sbjct: 55 SVGIHIPGGGVGVITEARCPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLRLNNNRLKAIPENFVTSSASLLRLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRL-APYTR---CQSPSQLKGQNVADLHDQEFKCSGLTEHAPMECGAENSCPHPCRCA-DGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIE-TSGARCESPKRMHRRRIESLREEKFKC-SWDELRMKLSGE-CRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAAR---CGAPSKVRDVQIKDLPHSEFKC-----SSENSEGCLGDGY---------------CPPSCTCTGTVVRCSRNQLKEIPRGIPAETSELYLESNEIEQIHYERIRHLRSLTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCGLKWFSDWIKLDYVEPGIARCAEPEQMKDKLILSTPSSSFVCRGRVRND-ILAKCNACFEQPCQNQAQCVALPQREYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEE-GRFSCQCAPGYTGARCETNIDDCLGEIKCQNNATCIDGVESYKCECQPGFSGEFCDTKIQFCSPEFNPCANGAKCMDHFTH-YSCDCQAGFHGTNCTDNIDDCQNHMCQNGGTCVDGINDYQCRCPDDYTGKYCE------------------GHNMISMMYPQT------SPCQNHEC-KHGVCFQPNAQGSDYLCRCHPGYTGKWCEYLTSISFVHNNSFVELEPLR---TRPEANVTIVFSSAEQNGILMYDGQD-AHLAVELFNGRIRVSYDVGNHPVS-TMYSFEMVADGKYHAVELLAIKKNFTLRVDRGLARSIINE--GSNDYLKLTTPMFLGGLPVDPAQQAYKNWQIRNLTSFKGCMKEVWINHKLVDFGN-AQRQQKITPGCA-LLEGEQQEEEDDEQDFMDETPHIKEEPVDPCLENKCRRGSRCVPNSNARD------GYQCKCKHGQRGRYCDQGEGSTEPPTVTAASTCRKEQVREYY----TENDCRSRQPLKYAKCVGGCGNQ---------------------CCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKKCY 1504
BLAST of slit vs. RefSeq Drosophila melanogaster
Match: gi|161077132|ref|NP_001097334.1| (slit, isoform E [Drosophila melanogaster]) HSP 1 Score: 742.265 bits (1915), Expect = 0.000e+0 Identity = 498/1549 (32.15%), Postives = 766/1549 (49.45%), Query Frame = 2
Query: 83 TIWINDPSEEVEI-----CPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXXQHLPDHLFSKLSKLQKLD------------------------LRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISEAI-TKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCANEENTCQPD---RKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXXTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGH-KCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDK-NPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNSLYF-GKDSHIEIHPPSRGFLMPKGNISLKFITNSSSGLILYQGFDKSYLVVNLFRGHIKISYNIGDKDIGPDGFSMDVVNDNKLHYLNIEIVGENLTMYLNGKVHFTLKSKYLMTNQYMALDTGIFIGGAPKKILNTAIQNHHFDNDSDFTGCIESLLINRKKIDFSKYMSKSVNVKPGCGDLISKTAQELPSSEVPTQDIEVPIKSTKSLPVKSTICSKPGVCSNNGVCHEVNEKNRKYKCVCKNGFVGRKCKK-----------RVSKCHGKTTRTFIKDPDTHDNCLSIKKFQYRVCKGTCSNNRMQADSNRNFYQRSTDNSLSPCCQPSKYTTHKIQFKCDNGRFYRRSVTLPRSCKCQGNCH 4588
++ I+ P V + CP+ C C +V+C HR +VP ++ RL L GN + VI ++DF +L L++LQL++N+I +IE +F +L++L +LRLN N ++ +P++ + + L +LD L NQ+ CL+EH F L L+ L L N L + I L LR LR+S N C+CHL WL+ FL+ + + P TR C +P +L ++ L EF+C+ + CP C C+ DGIV+C K LT++P LP +T EL L+ N IT L S ++ L++I L+ N + I A GL+QLT L L N ++ + F GL SL L L+ NEI C+ F + L LL L +N + +L + ++ ++L NP CDC + WL L + T A C P H ++SL+ F+C S +++ E C CP C C V+C+G GL +P I TT L+L+ N++ + + +LVKL+L NQ+ I+P+ F ++ ++E+ L NK+K ++N+ F L +L+ L L DN ISC+ SF+ +L L L +N +C+C L W +EW R G+ R C +P ++ + +L F C S E + C + CP C C T + CS QLKEIP IP T+ELYL+ N+++ ++ ++L +L L LS N IT ++ L L TL++S+NKLQC+ + A L+NL+VL L N +S +P +F DL +L +IALG NP +CDC +KW + + +++ GI+ CA PE+MK K I + + F+C + I AKC+AC + PCQN +C + +Y C C +HGK CE I+AC+G PCRN C + G + C C G+ G +C NIDDC G KC+N ATC+D V Y C+C GF G+FC++K +C + NPC N C + Y C C G+ G NC NIDDC+ ++C+N C D +N Y+C C Y G YCE + +S + S C HEC +GVC + C+C PGY+G +C+YL S+ F +S +E+ P P+ N+++ F + +G+++Y G D ++L V LF G I++SY++G+ + +S ++V D K H + + + +N T+ ++ + ++ ++ +N Y+ L T +F+GG P A +N N + F GC++ + IN K +DF + + PGC L+ QE E D IK P C + C N + Y+C CK+G GR C + S C + R + T ++C S + +Y C G C N CC K++ C N R Y +++ + R C C C+
Sbjct: 55 SVGIHIPGGGVGVITEARCPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLRLNNNRLKAIPENFVTSSASLLRLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRL-APYTR---CQSPSQLKGQNVADLHDQEFKCSGLTEHAPMECGAENSCPHPCRCA-DGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIE-TSGARCESPKRMHRRRIESLREEKFKC-SWDELRMKLSGE-CRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAAR---CGAPSKVRDVQIKDLPHSEFKC-----SSENSEGCLGDGY---------------CPPSCTCTGTVVRCSRNQLKEIPRGIPAETSELYLESNEIEQIHYERIRHLRSLTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCGLKWFSDWIKLDYVEPGIARCAEPEQMKDKLILSTPSSSFVCRGRVRND-ILAKCNACFEQPCQNQAQCVALPQREYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEE-GRFSCQCAPGYTGARCETNIDDCLGEIKCQNNATCIDGVESYKCECQPGFSGEFCDTKIQFCSPEFNPCANGAKCMDHFTH-YSCDCQAGFHGTNCTDNIDDCQNHMCQNGGTCVDGINDYQCRCPDDYTGKYCE------------------GHNMISMMYPQT------SPCQNHEC-KHGVCFQPNAQGSDYLCRCHPGYTGKWCEYLTSISFVHNNSFVELEPLR---TRPEANVTIVFSSAEQNGILMYDGQD-AHLAVELFNGRIRVSYDVGNHPVS-TMYSFEMVADGKYHAVELLAIKKNFTLRVDRGLARSIINE--GSNDYLKLTTPMFLGGLPVDPAQQAYKNWQIRNLTSFKGCMKEVWINHKLVDFGN-AQRQQKITPGCA-LLEGEQQEEEDDEQDFMDETPHIKEEPVDPCLENKCRRGSRCVPNSNARD------GYQCKCKHGQRGRYCDQGEGSTEPPTVTAASTCRKEQVREYY----TENDCRSRQPLKYAKCVGGCGNQ---------------------CCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKKCY 1504 HSP 2 Score: 742.265 bits (1915), Expect = 0.000e+0 Identity = 498/1549 (32.15%), Postives = 766/1549 (49.45%), Query Frame = -2
Query: 5043 TIWINDPSEEVEI-----CPKGCQCENFSVECKHRLFRNVPNILAEKTTRLNLLGNRIDVIRKSDFFKLNYLKVLQLSNNRISSIESGAFDXXXXXXXXXXXXXXIQHLPDHLFSKLSKLQKLD------------------------LRENQLQCLNEHVFSKLGMLKTLLLEGNKLYWISEAI-TKLKHLRELRISSNKLHCNCHLHWLNVFLKLKSDIFKPDTRSVVCVTPQRLSQIPISTLSTFEFQCANEENTCQPD---RKVCPKQCTCSQDGIVNCTGKQLTNIPQDLPTNTIELDLQNNSITILRQGFXXXXXXXXXXXLTQNGMVDIEPGAFIGLQQLTALYLNMNNLRTIRRNAFNGLSSLMFLYLHNNEIECLPTGTFEGVKRLYLLHLSENKLSALKEEVLRPLVKLRYLYLVHNPLYCDCKILWLVQVLKLKEVNATPVASCVGPSSAHGIAVKSLQNYNFQCNSESDIKYDTHDENCVPKVICPHKCSCSSEIVNCSGVGLSSLPEKIFPSTTTLILSHNDIKNLDQIYSVHQASNLVKLDLSNNQIENIKPDTFSKSTKLKEINLSSNKLKCVNNETFKHLIELEILQLNDNHISCIGTNSFKFNTNLKYLMLNNNELHCDCRLKWLSEWSRNNYGILGSLRLPTCQSPPILKGTPLPNLDEKYFVCEGNKSSKEETSSCAKANFSYSCCLDMKCNKIPKCPSKCKCESTRIDCSELQLKEIPTEIPPNTTELYLDRNDLKTLNESSFKNLINLKTLVLSYNGITEINKDVLTPLKNLETLVLSFNKLQCIHQDAFKDLSNLKVLILQSNDVSTIPFQAFRDLTNLNNIALGQNPFHCDCNIKWLNQFFLDRFLDNGISLCASPERMKLKSIYHSKPTEFICSEETEDTYITAKCDACMKTPCQNNGKCSLITHHDYICDCPNPFHGKSCEKRINACFGQPCRNGGICKNTDNFGNYECVCPSGFRGPKCTENIDDCGGH-KCENGATCVDQVNKYSCKCPNGFRGKFCESKFLYCHDK-NPCENSGHCEPLSNNDYKCSCTKGWEGKNCEQNIDDCKYNVCKNEAKCEDLVNGYKCICRPGYVGTYCEEPAWKPYKLKTNLSRRDIDRSEVSKHFTSINEVISESVCAYHECLNNGVCVNEDMNKQKSYCKCKPGYSGPFCQYLNSLYF-GKDSHIEIHPPSRGFLMPKGNISLKFITNSSSGLILYQGFDKSYLVVNLFRGHIKISYNIGDKDIGPDGFSMDVVNDNKLHYLNIEIVGENLTMYLNGKVHFTLKSKYLMTNQYMALDTGIFIGGAPKKILNTAIQNHHFDNDSDFTGCIESLLINRKKIDFSKYMSKSVNVKPGCGDLISKTAQELPSSEVPTQDIEVPIKSTKSLPVKSTICSKPGVCSNNGVCHEVNEKNRKYKCVCKNGFVGRKCKK-----------RVSKCHGKTTRTFIKDPDTHDNCLSIKKFQYRVCKGTCSNNRMQADSNRNFYQRSTDNSLSPCCQPSKYTTHKIQFKCDNGRFYRRSVTLPRSCKCQGNCH 9548
++ I+ P V + CP+ C C +V+C HR +VP ++ RL L GN + VI ++DF +L L++LQL++N+I +IE +F +L++L +LRLN N ++ +P++ + + L +LD L NQ+ CL+EH F L L+ L L N L + I L LR LR+S N C+CHL WL+ FL+ + + P TR C +P +L ++ L EF+C+ + CP C C+ DGIV+C K LT++P LP +T EL L+ N IT L S ++ L++I L+ N + I A GL+QLT L L N ++ + F GL SL L L+ NEI C+ F + L LL L +N + +L + ++ ++L NP CDC + WL L + T A C P H ++SL+ F+C S +++ E C CP C C V+C+G GL +P I TT L+L+ N++ + + +LVKL+L NQ+ I+P+ F ++ ++E+ L NK+K ++N+ F L +L+ L L DN ISC+ SF+ +L L L +N +C+C L W +EW R G+ R C +P ++ + +L F C S E + C + CP C C T + CS QLKEIP IP T+ELYL+ N+++ ++ ++L +L L LS N IT ++ L L TL++S+NKLQC+ + A L+NL+VL L N +S +P +F DL +L +IALG NP +CDC +KW + + +++ GI+ CA PE+MK K I + + F+C + I AKC+AC + PCQN +C + +Y C C +HGK CE I+AC+G PCRN C + G + C C G+ G +C NIDDC G KC+N ATC+D V Y C+C GF G+FC++K +C + NPC N C + Y C C G+ G NC NIDDC+ ++C+N C D +N Y+C C Y G YCE + +S + S C HEC +GVC + C+C PGY+G +C+YL S+ F +S +E+ P P+ N+++ F + +G+++Y G D ++L V LF G I++SY++G+ + +S ++V D K H + + + +N T+ ++ + ++ ++ +N Y+ L T +F+GG P A +N N + F GC++ + IN K +DF + + PGC L+ QE E D IK P C + C N + Y+C CK+G GR C + S C + R + T ++C S + +Y C G C N CC K++ C N R Y +++ + R C C C+
Sbjct: 55 SVGIHIPGGGVGVITEARCPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLRLNNNRLKAIPENFVTSSASLLRLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRL-APYTR---CQSPSQLKGQNVADLHDQEFKCSGLTEHAPMECGAENSCPHPCRCA-DGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIE-TSGARCESPKRMHRRRIESLREEKFKC-SWDELRMKLSGE-CRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAAR---CGAPSKVRDVQIKDLPHSEFKC-----SSENSEGCLGDGY---------------CPPSCTCTGTVVRCSRNQLKEIPRGIPAETSELYLESNEIEQIHYERIRHLRSLTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCGLKWFSDWIKLDYVEPGIARCAEPEQMKDKLILSTPSSSFVCRGRVRND-ILAKCNACFEQPCQNQAQCVALPQREYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEE-GRFSCQCAPGYTGARCETNIDDCLGEIKCQNNATCIDGVESYKCECQPGFSGEFCDTKIQFCSPEFNPCANGAKCMDHFTH-YSCDCQAGFHGTNCTDNIDDCQNHMCQNGGTCVDGINDYQCRCPDDYTGKYCE------------------GHNMISMMYPQT------SPCQNHEC-KHGVCFQPNAQGSDYLCRCHPGYTGKWCEYLTSISFVHNNSFVELEPLR---TRPEANVTIVFSSAEQNGILMYDGQD-AHLAVELFNGRIRVSYDVGNHPVS-TMYSFEMVADGKYHAVELLAIKKNFTLRVDRGLARSIINE--GSNDYLKLTTPMFLGGLPVDPAQQAYKNWQIRNLTSFKGCMKEVWINHKLVDFGN-AQRQQKITPGCA-LLEGEQQEEEDDEQDFMDETPHIKEEPVDPCLENKCRRGSRCVPNSNARD------GYQCKCKHGQRGRYCDQGEGSTEPPTVTAASTCRKEQVREYY----TENDCRSRQPLKYAKCVGGCGNQ---------------------CCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKKCY 1504 The following BLAST results are available for this feature:
BLAST of slit vs. uniprot
Analysis Date: 2016-03-07 (Dugesia dorotocephala BLASTX Swissprot Uniprot) Total hits: 5
BLAST of slit vs. Smed Unigenes AA
Analysis Date: 2016-03-08 (Dugesia dorotocephala BLASTX Schmidtea mediterranea) Total hits: 5
BLAST of slit vs. RefSeq Human
Analysis Date: 2016-03-08 (Dugesia dorotocephala BLASTX Human) Total hits: 5
BLAST of slit vs. RefSeq Drosophila melanogaster
Analysis Date: 2016-03-08 (Dugesia dorotocephala BLASTX Drosophila melanogaster) Total hits: 5
Sequences
The following sequences are available for this feature:
mRNA sequence >Ddo_016416 ID=Ddo_016416|Name=slit|organism=Dugesia dorotocephala|type=mRNA|length=9630bp Design Primers for slit back to top protein sequence >Ddo_016416-protein ID=Ddo_016416-protein|Name=slit|organism=Dugesia dorotocephala|type=polypeptide|length=1532bpback to top coding sequence >Ddo_016416.2.4597 ID=Ddo_016416.2.4597|Name=Ddo_016416.2.4597|organism=Dugesia dorotocephala|type=CDS|length=4596bpback to top Gene Groups
slit is similar in sequence to the genes of this group: GG9051
Gene Group Protein Sequences >SMU15036778 |
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