sfrp-1, Ddo_009225 (mRNA) Dugesia dorotocephala

Overview
Namesfrp-1
Unique NameDdo_009225
TypemRNA
OrganismDugesia dorotocephala (D. dorotocephala)
Sequence length1153
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Dugesia dorotocephala Transcriptome2016-03-03
Dugesia dorotocephala BLASTX Human2016-03-08
Dugesia dorotocephala BLASTX Drosophila melanogaster2016-03-08
Dugesia dorotocephala BLASTX Swissprot Uniprot2016-03-07
Dugesia dorotocephala BLASTX Schmidtea mediterranea2016-03-08
Dugesia dorotocephala CDS2016-04-26
Properties
Property NameValue
NoteSfrp-1
Homology
[Close]
BLAST of sfrp-1 vs. uniprot
Match: gi|61216765|sp|P97299|SFRP2_MOUSE (RecName: Full=Secreted frizzled-related protein 2; Short=sFRP-2; AltName: Full=Protein SDF5; AltName: Full=Secreted apoptosis-related protein 1; Short=SARP-1; Flags: Precursor)

HSP 1 Score: 140.198 bits (352), Expect = 9.361e-37
Identity = 74/200 (37.00%), Postives = 103/200 (51.50%), Query Frame = 3 
Query:  126 GYTTDQCYKVPENFSMCHNVGYNKMVLPNFIQHEYMEEAIAHSRVWLSLVKSQCHLDVRKFLCSLYAPVCITNKRIQKVPPCRELCIIVKEACLPSMKMYGFNWPEIMKCSKFPPVQNALCI---------PKTTVKKKNCELC-VKNAPYEVIANRFCLSPIVIRAKIKRIIPKESGFQIVLHQKSKFL-KFQSNSSRD 692
             Y    C  +P N  +CH + Y  M LPN + HE M+E +  +  W+ LV  QCH D +KFLCSL+APVC+ +   + + PC  LC+ VK+ C P M  +GF WP++++C +F P  N LCI         P T    K CE C  KN     I    C +   ++ K+K I       +I+L  KSK + K    S RD
Sbjct:   34 SYKRSNCKPIPANLQLCHGIEYQNMRLPNLLGHETMKEVLEQAGAWIPLVMKQCHPDTKKFLCSLFAPVCLDDLD-ETIQPCHSLCVQVKDRCAPVMSAFGFPWPDMLECDRF-PQDNDLCIPLASSDHLLPATEEAPKVCEACKTKNEDDNDIMETLCKNDFALKIKVKEITYINRDTKIILETKSKTIYKLNGVSERD 231
[Close]
BLAST of sfrp-1 vs. uniprot
Match: gi|61216791|sp|Q863H1|SFRP2_CANFA (RecName: Full=Secreted frizzled-related protein 2; Short=sFRP-2; Flags: Precursor [Canis lupus familiaris])

HSP 1 Score: 139.428 bits (350), Expect = 1.898e-36
Identity = 74/200 (37.00%), Postives = 103/200 (51.50%), Query Frame = 3 
Query:  126 GYTTDQCYKVPENFSMCHNVGYNKMVLPNFIQHEYMEEAIAHSRVWLSLVKSQCHLDVRKFLCSLYAPVCITNKRIQKVPPCRELCIIVKEACLPSMKMYGFNWPEIMKCSKFPPVQNALCI---------PKTTVKKKNCELCV-KNAPYEVIANRFCLSPIVIRAKIKRIIPKESGFQIVLHQKSKFL-KFQSNSSRD 692
             Y    C  +P N  +CH + Y  M LPN + HE M+E +  +  W+ LV  QCH D +KFLCSL+APVC+ +   + + PC  LC+ VK+ C P M  +GF WP++++C +F P  N LCI         P T    K CE C  KN     I    C +   ++ K+K I       +I+L  KSK + K    S RD
Sbjct:   33 SYKRSNCKPIPANLQLCHGIEYQNMRLPNLLGHETMKEVLEQAGAWIPLVMKQCHPDTKKFLCSLFAPVCLDDLD-ETIQPCHSLCVQVKDRCAPVMSAFGFPWPDMLECDRF-PQDNDLCIPLASSDHLLPATEEAPKVCEACKNKNEDDNDIMETLCKNDFALKIKVKEITYINRDTKIILETKSKTIYKLNGVSERD 230
[Close]
BLAST of sfrp-1 vs. uniprot
Match: gi|61216828|sp|Q96HF1|SFRP2_HUMAN (RecName: Full=Secreted frizzled-related protein 2; Short=FRP-2; Short=sFRP-2; AltName: Full=Secreted apoptosis-related protein 1; Short=SARP-1; Flags: Precursor)

HSP 1 Score: 139.428 bits (350), Expect = 1.919e-36
Identity = 74/200 (37.00%), Postives = 103/200 (51.50%), Query Frame = 3 
Query:  126 GYTTDQCYKVPENFSMCHNVGYNKMVLPNFIQHEYMEEAIAHSRVWLSLVKSQCHLDVRKFLCSLYAPVCITNKRIQKVPPCRELCIIVKEACLPSMKMYGFNWPEIMKCSKFPPVQNALCI---------PKTTVKKKNCELCV-KNAPYEVIANRFCLSPIVIRAKIKRIIPKESGFQIVLHQKSKFL-KFQSNSSRD 692
             Y    C  +P N  +CH + Y  M LPN + HE M+E +  +  W+ LV  QCH D +KFLCSL+APVC+ +   + + PC  LC+ VK+ C P M  +GF WP++++C +F P  N LCI         P T    K CE C  KN     I    C +   ++ K+K I       +I+L  KSK + K    S RD
Sbjct:   34 SYKRSNCKPIPANLQLCHGIEYQNMRLPNLLGHETMKEVLEQAGAWIPLVMKQCHPDTKKFLCSLFAPVCLDDLD-ETIQPCHSLCVQVKDRCAPVMSAFGFPWPDMLECDRF-PQDNDLCIPLASSDHLLPATEEAPKVCEACKNKNDDDNDIMETLCKNDFALKIKVKEITYINRDTKIILETKSKTIYKLNGVSERD 231
[Close]
BLAST of sfrp-1 vs. uniprot
Match: gi|341942099|sp|Q8C4U3|SFRP1_MOUSE (RecName: Full=Secreted frizzled-related protein 1; Short=sFRP-1; Flags: Precursor)

HSP 1 Score: 138.658 bits (348), Expect = 5.874e-36
Identity = 74/186 (39.78%), Postives = 102/186 (54.84%), Query Frame = 3 
Query:   99 IGDWESFRSGYTTDQCYKVPENFSMCHNVGYNKMVLPNFIQHEYMEEAIAHSRVWLSLVKSQCHLDVRKFLCSLYAPVCITNKRIQKVPPCRELCIIVKEACLPSMKMYGFNWPEIMKCSKFPPVQNALCI----PKTTVKKKN-----CELCVKNAPYEVIANRFCLSPIVIRAKIKRIIPKESG 629
            IG ++S R      QC  +P +  +CHNVGY KMVLPN ++HE M E    +  W+ L+   CH+  + FLCSL+APVC+     + + PCR LC  V+++C P M+ +GF WPE++KC KFP  +  +CI    P TT   K      C  C      E I    C S   +R KIK  + KE+G
Sbjct:   43 IGSYQSGRFYTKPPQCVDIPVDLRLCHNVGYKKMVLPNLLEHETMAEVKQQASSWVPLLNKNCHMGTQVFLCSLFAPVCLD----RPIYPCRWLCEAVRDSCEPVMQFFGFYWPEMLKCDKFP--EGDVCIAMTPPNTTEASKPQGTTVCPPCDNELKSEAIIEHLCASEFALRMKIKE-VKKENG 221
[Close]
BLAST of sfrp-1 vs. uniprot
Match: gi|61216839|sp|Q9DEQ4|SFRP1_CHICK (RecName: Full=Secreted frizzled-related protein 1; Short=CsFRP1; Short=sFRP-1; Flags: Precursor)

HSP 1 Score: 138.272 bits (347), Expect = 9.321e-36
Identity = 72/200 (36.00%), Postives = 107/200 (53.50%), Query Frame = 3 
Query:   99 IGDWESFRSGYTTDQCYKVPENFSMCHNVGYNKMVLPNFIQHEYMEEAIAHSRVWLSLVKSQCHLDVRKFLCSLYAPVCITNKRIQKVPPCRELCIIVKEACLPSMKMYGFNWPEIMKCSKFPPVQNALCIPKTTVKKKN---------CELCVKNAPYEVIANRFCLSPIVIRAKIKRIIPKESGFQIVLHQKSKFLKF 671
            +G +   R      QC  +P +  +CH+VGY+KMVLPN + HE M E    +  W+ L+   CH+  + FLCSL+APVC+     + V PCR LC  V+++C P M+ +GF WPE++KC +FP  Q+ +CI  TT              C  C      E I    C S   ++  IK +  KE+G ++++ +K K LK 
Sbjct:   43 LGPYPGGRFYTKPHQCVAIPADLRLCHSVGYDKMVLPNLLDHETMAEVKHQASSWVPLLNKNCHMGTQVFLCSLFAPVCLD----RPVYPCRWLCEAVRDSCEPVMQFFGFFWPEMLKCDQFP--QDYVCIAMTTPNATEVSRPKGTTVCPPCDNEMKSEAIVEHLCASEFALKMTIKEVK-KENGDKVIIPRKRKALKL 235
[Close]
BLAST of sfrp-1 vs. Smed Unigenes AA
Match: SMU15001274 (Asxlregen_comp63361_c0_seq1|m.13574|m.6037)

HSP 1 Score: 394.43 bits (1012), Expect = 3.328e-135
Identity = 209/299 (69.90%), Postives = 256/299 (85.62%), Query Frame = 3 
Query:   81 SNGNCFIGDWESFRSGYTTDQCYKVPENFSMCHNVGYNKMVLPNFIQHEYMEEAIAHSRVWLSLVKSQCHLDVRKFLCSLYAPVCITNKRIQKVPPCRELCIIVKEACLPSMKMYGFNWPEIMKCSKFPPVQNALCIPKTTVKKKNCELCVKNAPYEVIANRFCLSPIVIRAKIKRIIPKE-SGFQIVLHQKSKFLKFQSNSSRDSVXXXXXXXXXXXXFQQRALRGRWIIMARIVED-RFVVXXXXXXXXXXXXXXXXXRIIQKFGNVVCKSKLKDGISLQERYVAYMKMNNKTNYQN 971
            SN N FIGDW++F+SGYT DQCY++P+NF++C NVGY  MVLPN+IQHE +EEA  HS+VW+ LV +QCH+D++KF+CSLYAPVCI+N+R QKVPPCRELC+ VKE+CLPSMKMYGF+WP IMKCSKFP  +N+LCIPKT V  K C+LC+K A YEVIANRFCLSPIVIRAKIKRIIP   +  QI+L +KSKFLKFQ+N S++++ L LKCNC NLKF+QRAL+GRWIIMA+I  D + VVNFISKWK+KK EFK+S++IIQ FG++VCKSKL DGISL+ERY AY+KMNN+  Y N
Sbjct:   16 SNVNSFIGDWQAFQSGYTIDQCYQIPDNFTLCSNVGYRLMVLPNYIQHESIEEAAQHSKVWMGLVNTQCHVDIKKFICSLYAPVCISNQRNQKVPPCRELCVTVKESCLPSMKMYGFDWPTIMKCSKFPQFKNSLCIPKTVVPGKKCDLCMKIASYEVIANRFCLSPIVIRAKIKRIIPTSGNAIQIILQKKSKFLKFQANFSQNALELNLKCNCTNLKFKQRALKGRWIIMAQIDSDNKAVVNFISKWKRKKAEFKHSMKIIQNFGHIVCKSKLPDGISLRERYAAYLKMNNQLKYSN 314
[Close]
BLAST of sfrp-1 vs. Smed Unigenes AA
Match: SMU15040524 (dd_smedV4_8832_0_1|m.39951|m.18186)

HSP 1 Score: 189.119 bits (479), Expect = 1.036e-55
Identity = 114/302 (37.75%), Postives = 176/302 (58.28%), Query Frame = 3 
Query:   99 IGDWESFRSGYTTDQCYKVPENFSMCHNVGYNKMVLPNFIQHEYMEEAIAHSRVWLSLVKSQCHLDVRKFLCSLYAPVCITNKRIQKVPPCRELCIIVKEACLPSMKMYGFNWPEIMKCSKFPPVQNALCIPKT---------TVKKKNCELCVKNAPYEVIANRFCLSPIVIRAKIKRI-IPKESGFQIVLHQKSKFLKFQSNSSRDSVXXXXXXXXXXXXFQQRALR-GRWIIMARIVEDRFVVXXXXXXXXXXXXXXXXXRIIQKFGNVVCKSKLKDGISLQERYVAYMKMNNKTNYQN 971
            + +W +   GY++ +C+++P  F +C N+GYNKM+LPNF+QHE ++EAI  S  W      +CH D R+FLCSLYAPVC+ N+ ++K+ PC+ELC+ VK+AC P+M  +G+ WPEI+ C KFP     LCI  T         +V  K C  C        I + +C S  VIRAKI R+ I   S + + LH+KSK +K  SN +       ++ +CL        +R GRW++M  + + +F +  ++KWKKK ++FK  IR ++KF   +C S+ +      +R ++ + +N     QN
Sbjct:   25 VPEWNNLIYGYSSHRCHEIPNEFGLCKNIGYNKMILPNFLQHETVQEAINSSIDWQKFADIKCHNDTRRFLCSLYAPVCVPNEPVRKIQPCKELCLSVKKACSPNMLTFGYKWPEILHCDKFPQKFAGLCIRGTEGAVPSGSSSVVPKGCN-CRSLYSAAFINDNYCSSTTVIRAKISRLRIRNNSKYVMRLHKKSKIIK--SNPTDLPFITSIEVSCLCDMIDNDNIRKGRWLLMGVVNDGKFTLTSLTKWKKKNKDFKKVIRKLKKFEEKICGSRFR----ASDRKMSGLNINGGKMSQN 319
[Close]
BLAST of sfrp-1 vs. Smed Unigenes AA
Match: SMU15030883 (SmedSxlregen_c55322_g1_i1|m.6956|m.30359)

HSP 1 Score: 139.043 bits (349), Expect = 8.799e-37
Identity = 87/270 (32.22%), Postives = 126/270 (46.67%), Query Frame = 3 
Query:  114 SFRSGYT---TDQCYKVPENFSMCHNVG-YNKM-VLPNFIQHEYMEEAIAHSRVWLSLVKSQCHLDVRKFLCSLYAPVCITNKRIQKVPPCRELCIIVKEACLPSMKMYGFNWPEIMKCSKFPPVQNALCIPKT-------------------------------TVKKKNCELCVKNAPYEVIANRFCLSPIVIRAKIKRIIPKESG-FQIVLHQKSKFLKFQSNSSRDSVXXXXXXXXXXXXFQQRA---LRGRWIIMARIVEDRFVV 803
             FR G      +QCY    N ++C   G  N M      +++     +I  S +W+ L+ ++CH+D+ KFLC++YAP+C+   R  +V PC ELC  VKE CLP +K+YGF WP I+ C KFP    A CI  T                               T++KKNC +C +  P E++   FC   +VIRAKI  I  K  G   I L  KS  +   S+  R S+   +          Q A    +G+W++   I   +F+V
Sbjct:   20 DFRVGANRQRLNQCYPKFANHTLCGGAGVLNSMKTFSGLLRNSKSGFSIYQSNIWIILLHTKCHVDLEKFLCTIYAPICLKGHRFTRVFPCEELCASVKEQCLPRLKIYGFEWPSIVDCGKFPLENKASCIQLTDGTGNYLKKSLVKKQREDRSLNQTILSDQEQTLQKKNCPICNRKVPLEILTKNFCKYSLVIRAKIANIFQKPKGAISIRLQHKSNII-HSSHPIRISLANLITEIQCKCSILQNAKSVSKGKWLLFGDIENGQFIV 288
[Close]
BLAST of sfrp-1 vs. Smed Unigenes AA
Match: SMU15001054 (Asxlregen_comp62015_c0_seq1|m.11130|m.22104)

HSP 1 Score: 102.834 bits (255), Expect = 9.958e-24
Identity = 56/153 (36.60%), Postives = 83/153 (54.25%), Query Frame = 3 
Query:  153 VPENFSMCHNVGYNKMVLPNFIQHEYMEEAIAHSRVWLSLVKSQCHLDVRKFLCSLYAPVCITNKRIQKVPPCRELCIIVKEACLPSMKMYGFNWPEIMKCSKFPPVQNALCIPKTTVKKKN-CELCVKNAPYEVIANR---FCLSPIVIRAK 599
            +P   SMC  +GYN   +PN + H  +EEA    + + +LV+  C  D+R FLCS+YAP+C+ N    ++P CR LCI  +E C   M  YGF WPE M C K P  ++  C+ K    + N     V   PY + + +    C SP++I ++
Sbjct:   36 IPIEISMCKGIGYNLTYIPNDLNHASLEEAGLEIQQFNALVEINCSEDLRLFLCSMYAPICLPNWP-NRIPACRSLCISAREGCTRVMNQYGFKWPEGMNCEKLP--EDNACLRKDNKTRSNKTANFVSERPYLLKSKKCHCHCRSPLIIDSR 185
[Close]
BLAST of sfrp-1 vs. Smed Unigenes AA
Match: SMU15036199 (SmedSxlregen_c90692_g1_i1|m.42870|m.18187)

HSP 1 Score: 94.3597 bits (233), Expect = 7.942e-21
Identity = 56/139 (40.29%), Postives = 71/139 (51.08%), Query Frame = 3 
Query:  153 VPENFSMCHNVGYNKMVLPNFIQHEYMEEAIAHSRVWLSLVKSQCHLDVRKFLCSLYAPVCITN--KRIQKVPPCRELCIIVKEACLPSMKMYGFNWPEIMKCSKFPPVQNALCIPKTTVK-----KKNCELCVKNAPY 548
            VP    MC N+GYN   +PN   HE  EEA      +  LV+  C  ++R FLCSLYAP+C+ N  K IQ    CR +C   ++ C P M  YGF WP+ M C K    +N  C+ K T        K CE C   +PY
Sbjct:   60 VPIAIPMCKNIGYNLTYMPNEFHHETQEEAGLVVHQFYPLVEIACSDNLRLFLCSLYAPICLPNWPKPIQA---CRSVCESARDGCAPVMNTYGFKWPKSMDCDKL--TENQACLKKDTKNVTKPPSKGCE-CKCRSPY 192
[Close]
BLAST of sfrp-1 vs. RefSeq Human
Match: gi|48475052|ref|NP_003004.1| (secreted frizzled-related protein 2 precursor [Homo sapiens])

HSP 1 Score: 139.428 bits (350), Expect = 7.241e-37
Identity = 74/200 (37.00%), Postives = 103/200 (51.50%), Query Frame = 3 
Query:  126 GYTTDQCYKVPENFSMCHNVGYNKMVLPNFIQHEYMEEAIAHSRVWLSLVKSQCHLDVRKFLCSLYAPVCITNKRIQKVPPCRELCIIVKEACLPSMKMYGFNWPEIMKCSKFPPVQNALCI---------PKTTVKKKNCELCV-KNAPYEVIANRFCLSPIVIRAKIKRIIPKESGFQIVLHQKSKFL-KFQSNSSRD 692
             Y    C  +P N  +CH + Y  M LPN + HE M+E +  +  W+ LV  QCH D +KFLCSL+APVC+ +   + + PC  LC+ VK+ C P M  +GF WP++++C +F P  N LCI         P T    K CE C  KN     I    C +   ++ K+K I       +I+L  KSK + K    S RD
Sbjct:   34 SYKRSNCKPIPANLQLCHGIEYQNMRLPNLLGHETMKEVLEQAGAWIPLVMKQCHPDTKKFLCSLFAPVCLDDLD-ETIQPCHSLCVQVKDRCAPVMSAFGFPWPDMLECDRF-PQDNDLCIPLASSDHLLPATEEAPKVCEACKNKNDDDNDIMETLCKNDFALKIKVKEITYINRDTKIILETKSKTIYKLNGVSERD 231
[Close]
BLAST of sfrp-1 vs. RefSeq Human
Match: gi|56117838|ref|NP_003003.3| (secreted frizzled-related protein 1 precursor [Homo sapiens])

HSP 1 Score: 135.576 bits (340), Expect = 2.821e-35
Identity = 73/186 (39.25%), Postives = 100/186 (53.76%), Query Frame = 3 
Query:   99 IGDWESFRSGYTTDQCYKVPENFSMCHNVGYNKMVLPNFIQHEYMEEAIAHSRVWLSLVKSQCHLDVRKFLCSLYAPVCITNKRIQKVPPCRELCIIVKEACLPSMKMYGFNWPEIMKCSKFPPVQNALCI----PKTTVKKKN-----CELCVKNAPYEVIANRFCLSPIVIRAKIKRIIPKESG 629
            IG ++S R      QC  +P +  +CHNVGY KMVLPN ++HE M E    +  W+ L+   CH   + FLCSL+APVC+     + + PCR LC  V+++C P M+ +GF WPE++KC KFP  +  +CI    P  T   K      C  C      E I    C S   +R KIK +  KE+G
Sbjct:   43 IGPYQSGRFYTKPPQCVDIPADLRLCHNVGYKKMVLPNLLEHETMAEVKQQASSWVPLLNKNCHAGTQVFLCSLFAPVCLD----RPIYPCRWLCEAVRDSCEPVMQFFGFYWPEMLKCDKFP--EGDVCIAMTPPNATEASKPQGTTVCPPCDNELKSEAIIEHLCASEFALRMKIKEVK-KENG 221
[Close]
BLAST of sfrp-1 vs. RefSeq Human
Match: gi|188528609|ref|NP_003006.2| (secreted frizzled-related protein 5 precursor [Homo sapiens])

HSP 1 Score: 129.413 bits (324), Expect = 5.262e-33
Identity = 77/239 (32.22%), Postives = 116/239 (48.54%), Query Frame = 3 
Query:  120 RSGYTTDQCYKVPENFSMCHNVGYNKMVLPNFIQHEYMEEAIAHSRVWLSLVKSQCHLDVRKFLCSLYAPVCITNKRIQKVPPCRELCIIVKEACLPSMKMYGFNWPEIMKCSKFPPVQNALCI-------PKTTVK-KKNCELCVKNAPYEVIANRFCLSPIVIRAKIKRIIPKESGFQIVLHQKSKFLKFQSNSSRDSVXXXXXXXXXXXXF---QQRALRGRWIIMARIVEDRFVV 803
            RS     QC  +P +  +CH VGY +M LPN ++HE + E    +  WL L+  +CH D + FLCSL+APVC+     + + PCR LC  V+  C P M+ YGF WPE++ C KF P+ N LCI       P T     K C  C      + +  + C S  V++ +IK I  +    +++  QK K L       R      +            Q  +L G +++M R V+ + ++
Sbjct:   45 RSYSKPPQCLDIPADLPLCHTVGYKRMRLPNLLEHESLAEVKQQASSWLPLLAKRCHSDTQVFLCSLFAPVCLD----RPIYPCRSLCEAVRAGCAPLMEAYGFPWPEMLHCHKF-PLDNDLCIAVQFGHLPATAPPVTKICAQCEMEHSADGLMEQMCSSDFVVKMRIKEIKIENGDRKLIGAQKKKKLLKPGPLKRKDTKRLVLHMKNGAGCPCPQLDSLAGSFLVMGRKVDGQLLL 278
[Close]
BLAST of sfrp-1 vs. RefSeq Human
Match: gi|4503825|ref|NP_003496.1| (frizzled-1 [Homo sapiens])

HSP 1 Score: 100.908 bits (250), Expect = 2.634e-22
Identity = 49/129 (37.98%), Postives = 72/129 (55.81%), Query Frame = 3 
Query:  138 DQCYKVPENFSMCHNVGYNKMVLPNFIQHEYMEEAIAHSRVWLSLVKSQCHLDVRKFLCSLYAPVCITNKRIQKVPPCRELCIIVKEACLPSMKMYGFNWPEIMKCSKFPPVQNA--LCIPKTTVKKKN 518
            D  Y  P +  +C ++ YN+ ++PN + H   E+A      +  LVK QC  +++ FLCS+YAPVC   +  Q +PPCR LC   ++ C   M  +GF WP+ +KC KF PV  A  LC+ + T  K  
Sbjct:  112 DHGYCQPISIPLCTDIAYNQTIMPNLLGHTNQEDAGLEVHQFYPLVKVQCSAELKFFLCSMYAPVCTVLE--QALPPCRSLCERARQGCEALMNKFGFQWPDTLKCEKF-PVHGAGELCVGQNTSDKGT 237
[Close]
BLAST of sfrp-1 vs. RefSeq Human
Match: gi|4503833|ref|NP_003498.1| (frizzled-7 precursor [Homo sapiens])

HSP 1 Score: 95.1301 bits (235), Expect = 2.137e-20
Identity = 47/124 (37.90%), Postives = 69/124 (55.65%), Query Frame = 3 
Query:  138 DQCYKVPENFSMCHNVGYNKMVLPNFIQHEYMEEAIAHSRVWLSLVKSQCHLDVRKFLCSLYAPVCITNKRIQKVPPCRELCIIVKEACLPSMKMYGFNWPEIMKCSKFPPVQNA--LCIPKTT 503
            D  +  P +  +C ++ YN+ +LPN + H   E+A      +  LVK QC  ++R FLCS+YAPVC      Q +PPCR LC   ++ C   M  +GF WPE ++C  F PV  A  +C+ + T
Sbjct:   45 DHGFCQPISIPLCTDIAYNQTILPNLLGHTNQEDAGLEVHQFYPLVKVQCSPELRFFLCSMYAPVCTVLD--QAIPPCRSLCERARQGCEALMNKFGFQWPERLRCENF-PVHGAGEICVGQNT 165
[Close]
BLAST of sfrp-1 vs. RefSeq Drosophila melanogaster
Match: gi|442632294|ref|NP_001261836.1| (frizzled, isoform C [Drosophila melanogaster])

HSP 1 Score: 92.8189 bits (229), Expect = 3.934e-20
Identity = 48/104 (46.15%), Postives = 60/104 (57.69%), Query Frame = 3 
Query:  156 PENFSMCHNVGYNKMVLPNFIQHEYMEEAIAHSRVWLSLVKSQCHLDVRKFLCSLYAPVCITNKRIQKVPPCRELCIIVKEACLPSMKMYGFNWPEIMKCSKFP 467
            P   S+C N+ YN  ++PN I H   EEA      +  LVK  C  D++ FLCSLY PVC   +R   +PPCR LC   +  C   MK Y FNWPE ++CSKFP
Sbjct:   55 PITISICKNIPYNMTIMPNLIGHTKQEEAGLEVHQFAPLVKIGCSDDLQLFLCSLYVPVCTILER--PIPPCRSLCESAR-VCEKLMKTYNFNWPENLECSKFP 155
[Close]
BLAST of sfrp-1 vs. RefSeq Drosophila melanogaster
Match: gi|17864440|ref|NP_524812.1| (frizzled, isoform A [Drosophila melanogaster])

HSP 1 Score: 92.4337 bits (228), Expect = 4.178e-20
Identity = 48/104 (46.15%), Postives = 60/104 (57.69%), Query Frame = 3 
Query:  156 PENFSMCHNVGYNKMVLPNFIQHEYMEEAIAHSRVWLSLVKSQCHLDVRKFLCSLYAPVCITNKRIQKVPPCRELCIIVKEACLPSMKMYGFNWPEIMKCSKFP 467
            P   S+C N+ YN  ++PN I H   EEA      +  LVK  C  D++ FLCSLY PVC   +R   +PPCR LC   +  C   MK Y FNWPE ++CSKFP
Sbjct:   55 PITISICKNIPYNMTIMPNLIGHTKQEEAGLEVHQFAPLVKIGCSDDLQLFLCSLYVPVCTILER--PIPPCRSLCESAR-VCEKLMKTYNFNWPENLECSKFP 155
[Close]
BLAST of sfrp-1 vs. RefSeq Drosophila melanogaster
Match: gi|442633302|ref|NP_001262037.1| (frizzled 2, isoform F [Drosophila melanogaster])

HSP 1 Score: 83.9593 bits (206), Expect = 3.551e-17
Identity = 37/99 (37.37%), Postives = 54/99 (54.55%), Query Frame = 3 
Query:  171 MCHNVGYNKMVLPNFIQHEYMEEAIAHSRVWLSLVKSQCHLDVRKFLCSLYAPVCITNKRIQKVPPCRELCIIVKEACLPSMKMYGFNWPEIMKCSKFP 467
            MC  +GYN    PN + HE  +EA      +  LV+ +C  D++ FLCS+Y P+C+ +   + +P CR +C   +  C P M+ Y F WPE M C   P
Sbjct:   71 MCRGIGYNMTSFPNEMNHETQDEAGLEVHQFWPLVEIKCSPDLKFFLCSMYTPICLEDYH-KPLPVCRSVCERARSGCAPIMQQYSFEWPERMACEHLP 168
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BLAST of sfrp-1 vs. RefSeq Drosophila melanogaster
Match: gi|17737633|ref|NP_524155.1| (frizzled 2, isoform A [Drosophila melanogaster])

HSP 1 Score: 83.9593 bits (206), Expect = 3.632e-17
Identity = 37/99 (37.37%), Postives = 54/99 (54.55%), Query Frame = 3 
Query:  171 MCHNVGYNKMVLPNFIQHEYMEEAIAHSRVWLSLVKSQCHLDVRKFLCSLYAPVCITNKRIQKVPPCRELCIIVKEACLPSMKMYGFNWPEIMKCSKFP 467
            MC  +GYN    PN + HE  +EA      +  LV+ +C  D++ FLCS+Y P+C+ +   + +P CR +C   +  C P M+ Y F WPE M C   P
Sbjct:   71 MCRGIGYNMTSFPNEMNHETQDEAGLEVHQFWPLVEIKCSPDLKFFLCSMYTPICLEDYH-KPLPVCRSVCERARSGCAPIMQQYSFEWPERMACEHLP 168
[Close]
BLAST of sfrp-1 vs. RefSeq Drosophila melanogaster
Match: gi|24666637|ref|NP_730389.1| (frizzled 2, isoform B [Drosophila melanogaster])

HSP 1 Score: 83.9593 bits (206), Expect = 3.632e-17
Identity = 37/99 (37.37%), Postives = 54/99 (54.55%), Query Frame = 3 
Query:  171 MCHNVGYNKMVLPNFIQHEYMEEAIAHSRVWLSLVKSQCHLDVRKFLCSLYAPVCITNKRIQKVPPCRELCIIVKEACLPSMKMYGFNWPEIMKCSKFP 467
            MC  +GYN    PN + HE  +EA      +  LV+ +C  D++ FLCS+Y P+C+ +   + +P CR +C   +  C P M+ Y F WPE M C   P
Sbjct:   71 MCRGIGYNMTSFPNEMNHETQDEAGLEVHQFWPLVEIKCSPDLKFFLCSMYTPICLEDYH-KPLPVCRSVCERARSGCAPIMQQYSFEWPERMACEHLP 168
The following BLAST results are available for this feature:
BLAST of sfrp-1 vs. uniprot
Analysis Date: 2016-03-07 (Dugesia dorotocephala BLASTX Swissprot Uniprot)
Total hits: 5
Match NameE-valueIdentityDescription
gi|61216765|sp|P97299|SFRP2_MOUSE9.361e-3737.00RecName: Full=Secreted frizzled-related protein 2;... [more]
gi|61216791|sp|Q863H1|SFRP2_CANFA1.898e-3637.00RecName: Full=Secreted frizzled-related protein 2;... [more]
gi|61216828|sp|Q96HF1|SFRP2_HUMAN1.919e-3637.00RecName: Full=Secreted frizzled-related protein 2;... [more]
gi|341942099|sp|Q8C4U3|SFRP1_MOUSE5.874e-3639.78RecName: Full=Secreted frizzled-related protein 1;... [more]
gi|61216839|sp|Q9DEQ4|SFRP1_CHICK9.321e-3636.00RecName: Full=Secreted frizzled-related protein 1;... [more]
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BLAST of sfrp-1 vs. Smed Unigenes AA
Analysis Date: 2016-03-08 (Dugesia dorotocephala BLASTX Schmidtea mediterranea)
Total hits: 5
Match NameE-valueIdentityDescription
SMU150012743.328e-13569.90Asxlregen_comp63361_c0_seq1|m.13574|m.6037[more]
SMU150405241.036e-5537.75dd_smedV4_8832_0_1|m.39951|m.18186[more]
SMU150308838.799e-3732.22SmedSxlregen_c55322_g1_i1|m.6956|m.30359[more]
SMU150010549.958e-2436.60Asxlregen_comp62015_c0_seq1|m.11130|m.22104[more]
SMU150361997.942e-2140.29SmedSxlregen_c90692_g1_i1|m.42870|m.18187[more]
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BLAST of sfrp-1 vs. RefSeq Human
Analysis Date: 2016-03-08 (Dugesia dorotocephala BLASTX Human)
Total hits: 5
Match NameE-valueIdentityDescription
gi|48475052|ref|NP_003004.1|7.241e-3737.00secreted frizzled-related protein 2 precursor [Hom... [more]
gi|56117838|ref|NP_003003.3|2.821e-3539.25secreted frizzled-related protein 1 precursor [Hom... [more]
gi|188528609|ref|NP_003006.2|5.262e-3332.22secreted frizzled-related protein 5 precursor [Hom... [more]
gi|4503825|ref|NP_003496.1|2.634e-2237.98frizzled-1 [Homo sapiens][more]
gi|4503833|ref|NP_003498.1|2.137e-2037.90frizzled-7 precursor [Homo sapiens][more]
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BLAST of sfrp-1 vs. RefSeq Drosophila melanogaster
Analysis Date: 2016-03-08 (Dugesia dorotocephala BLASTX Drosophila melanogaster)
Total hits: 5
Match NameE-valueIdentityDescription
gi|442632294|ref|NP_001261836.1|3.934e-2046.15frizzled, isoform C [Drosophila melanogaster][more]
gi|17864440|ref|NP_524812.1|4.178e-2046.15frizzled, isoform A [Drosophila melanogaster][more]
gi|442633302|ref|NP_001262037.1|3.551e-1737.37frizzled 2, isoform F [Drosophila melanogaster][more]
gi|17737633|ref|NP_524155.1|3.632e-1737.37frizzled 2, isoform A [Drosophila melanogaster][more]
gi|24666637|ref|NP_730389.1|3.632e-1737.37frizzled 2, isoform B [Drosophila melanogaster][more]
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Sequences
The following sequences are available for this feature:

mRNA sequence

>Ddo_009225 ID=Ddo_009225|Name=sfrp-1|organism=Dugesia dorotocephala|type=mRNA|length=1153bp
ATTTAATCGCATTTTCAAATGATATTGGTATTCGGGGAATGATCACAATT
CTACCTCTAGGTTTATTGTTGTCGTTGTTTTCCAATGGGAATTGCTTTAT
TGGCGATTGGGAATCGTTTCGAAGCGGTTATACGACGGATCAATGTTACA
AAGTTCCCGAAAACTTTTCAATGTGTCATAATGTTGGTTATAACAAAATG
GTTCTACCTAATTTTATACAACACGAGTATATGGAAGAAGCGATAGCTCA
TTCTCGAGTATGGTTAAGTCTAGTTAAGTCTCAATGTCATCTTGATGTGA
GAAAGTTTTTGTGTTCTTTATATGCTCCTGTATGTATTACAAATAAAAGA
ATACAAAAAGTTCCCCCATGTCGAGAACTATGCATAATTGTCAAGGAAGC
TTGTTTACCAAGTATGAAGATGTACGGCTTTAACTGGCCGGAGATTATGA
AATGTTCCAAATTTCCACCAGTACAAAATGCATTATGTATACCGAAAACA
ACAGTGAAAAAAAAAAATTGTGAATTATGTGTTAAAAATGCGCCTTACGA
AGTGATAGCAAATAGATTTTGTTTATCTCCCATAGTAATAAGGGCTAAAA
TCAAACGAATTATTCCCAAAGAATCGGGGTTTCAAATAGTTTTGCATCAA
AAATCCAAATTTTTAAAATTTCAATCAAATTCTAGTCGGGATTCCGTCAA
TTTATGGCTGAAATGTAATTGTTTGAATCTGAAATTTCAGCAAAGAGCCC
TTCGAGGAAGGTGGATCATCATGGCTCGAATCGTCGAAGATAGATTTGTT
GTGAATTTCATTTCCAAATGGAAAAAAAAGAAAGAGGAATTTAAAAATTC
TATTAGAATTATTCAGAAATTCGGAAATGTTGTTTGTAAATCGAAGCTTA
AGGATGGCATAAGTCTCCAGGAACGATATGTGGCCTATATGAAGATGAAT
AATAAAACCAATTATCAAAATCAGTTGAAACAACAAACGAGAAAGTTTAA
GAAAAGAAAACAAAATCAACAACGAAGAATTCCTTAAAATTTTTCCTTCT
TGGTTTTTTTTAAATATAAAATATTAATTGCTTTATTAATTATGAAACAT
TGCATCGATTAAAGACTTAACATTATTTTTATTATGTAAACTTTATATGT
TCA

Design Primers for sfrp-1

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protein sequence

>Ddo_009225-protein ID=Ddo_009225-protein|Name=sfrp-1|organism=Dugesia dorotocephala|type=polypeptide|length=345bp

LIAFSNDIGIRGMITILPLGLLLSLFSNGNCFIGDWESFRSGYTTDQCYK
VPENFSMCHNVGYNKMVLPNFIQHEYMEEAIAHSRVWLSLVKSQCHLDVR
KFLCSLYAPVCITNKRIQKVPPCRELCIIVKEACLPSMKMYGFNWPEIMK
CSKFPPVQNALCIPKTTVKKKNCELCVKNAPYEVIANRFCLSPIVIRAKI
KRIIPKESGFQIVLHQKSKFLKFQSNSSRDSVNLWLKCNCLNLKFQQRAL
RGRWIIMARIVEDRFVVNFISKWKKKKEEFKNSIRIIQKFGNVVCKSKLK
DGISLQERYVAYMKMNNKTNYQNQLKQQTRKFKKRKQNQQRRIP*
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coding sequence

>Ddo_009225.3.1037 ID=Ddo_009225.3.1037|Name=Ddo_009225.3.1037|organism=Dugesia dorotocephala|type=CDS|length=1035bp
TTAATCGCATTTTCAAATGATATTGGTATTCGGGGAATGATCACAATTCT
ACCTCTAGGTTTATTGTTGTCGTTGTTTTCCAATGGGAATTGCTTTATTG
GCGATTGGGAATCGTTTCGAAGCGGTTATACGACGGATCAATGTTACAAA
GTTCCCGAAAACTTTTCAATGTGTCATAATGTTGGTTATAACAAAATGGT
TCTACCTAATTTTATACAACACGAGTATATGGAAGAAGCGATAGCTCATT
CTCGAGTATGGTTAAGTCTAGTTAAGTCTCAATGTCATCTTGATGTGAGA
AAGTTTTTGTGTTCTTTATATGCTCCTGTATGTATTACAAATAAAAGAAT
ACAAAAAGTTCCCCCATGTCGAGAACTATGCATAATTGTCAAGGAAGCTT
GTTTACCAAGTATGAAGATGTACGGCTTTAACTGGCCGGAGATTATGAAA
TGTTCCAAATTTCCACCAGTACAAAATGCATTATGTATACCGAAAACAAC
AGTGAAAAAAAAAAATTGTGAATTATGTGTTAAAAATGCGCCTTACGAAG
TGATAGCAAATAGATTTTGTTTATCTCCCATAGTAATAAGGGCTAAAATC
AAACGAATTATTCCCAAAGAATCGGGGTTTCAAATAGTTTTGCATCAAAA
ATCCAAATTTTTAAAATTTCAATCAAATTCTAGTCGGGATTCCGTCAATT
TATGGCTGAAATGTAATTGTTTGAATCTGAAATTTCAGCAAAGAGCCCTT
CGAGGAAGGTGGATCATCATGGCTCGAATCGTCGAAGATAGATTTGTTGT
GAATTTCATTTCCAAATGGAAAAAAAAGAAAGAGGAATTTAAAAATTCTA
TTAGAATTATTCAGAAATTCGGAAATGTTGTTTGTAAATCGAAGCTTAAG
GATGGCATAAGTCTCCAGGAACGATATGTGGCCTATATGAAGATGAATAA
TAAAACCAATTATCAAAATCAGTTGAAACAACAAACGAGAAAGTTTAAGA
AAAGAAAACAAAATCAACAACGAAGAATTCCTTAA
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Gene Groups
sfrp-1 is similar in sequence to the genes of this group: GG7656
Gene NameGene ID
SMU15001274SMU15001274
sfrp-1Ddo_009225
sfrp-1Gsp_013592
sfrp-1Pgr_015361
sfrp-1Pmo_009874

Gene Group Protein Sequences

>SMU15001274

MEMTKIFPLSLLLFISNVNSFIGDWQAFQSGYTIDQCYQIPDNFTLCSNV
GYRLMVLPNYIQHESIEEAAQHSKVWMGLVNTQCHVDIKKFICSLYAPVC
ISNQRNQKVPPCRELCVTVKESCLPSMKMYGFDWPTIMKCSKFPQFKNSL
CIPKTVVPGKKCDLCMKIASYEVIANRFCLSPIVIRAKIKRIIPTSGNAI
QIILQKKSKFLKFQANFSQNALELNLKCNCTNLKFKQRALKGRWIIMAQI
DSDNKAVVNFISKWKRKKAEFKHSMKIIQNFGHIVCKSKLPDGISLRERY
AAYLKMNNQLKYSNERKLQTRKFRKQKSRKSVKPNNRSAGIS*

>Ddo_009225 sfrp-1

LIAFSNDIGIRGMITILPLGLLLSLFSNGNCFIGDWESFRSGYTTDQCYK
VPENFSMCHNVGYNKMVLPNFIQHEYMEEAIAHSRVWLSLVKSQCHLDVR
KFLCSLYAPVCITNKRIQKVPPCRELCIIVKEACLPSMKMYGFNWPEIMK
CSKFPPVQNALCIPKTTVKKKNCELCVKNAPYEVIANRFCLSPIVIRAKI
KRIIPKESGFQIVLHQKSKFLKFQSNSSRDSVNLWLKCNCLNLKFQQRAL
RGRWIIMARIVEDRFVVNFISKWKKKKEEFKNSIRIIQKFGNVVCKSKLK
DGISLQERYVAYMKMNNKTNYQNQLKQQTRKFKKRKQNQQRRIP*

>Gsp_013592 sfrp-1

MVIIFRFTFILFVFNVNCFIGDWQSFQSGYTIDQCYHIPDNFSLCRNVGY
NRMVLPNYIQHESIEEAVQHSRVWMGLVNTQCHIDIRKFICSLYAPVCIS
SQRVQKVPPCRELCVTVKESCLPSMKMYGFDWPTIMKCSKFPQFQNSLCI
PRTLVPEKNCDLCIKNPSYEVIANRFCLSPIVIRAKIKRIIPIGSKTMQI
VLQKKSKFLKFFANFSQTDLNLRLKCNCTNLKFKQRALKGRWIIMAKVNQ
NEAVVNFITKWKRKKAEFKHSMKIIQNFGHIVCKSKLPDGISLRERYAAY
LKMNNKIKYSNNNNKSKQQTRRSKKPINTRSSSIS*

>Pgr_015361 sfrp-1

LFLFFIFYLHISCFLGDWQQFQNGYSTEQCFKIPDNLTLCLNVGYKRMIL
PNYIQHEYLDEAVQHSKVWIGLVNSHCHEDIRKFLCSLYAPVCINNMRIQ
KVPPCKELCVSVKEACLPSMKLFGFEWPNIMKCSLFPSVRNSLCIPSTVS
KGKKCRPCVKSSSYEVMANKFCLSNIVIRAKIKRIVKEGNNGFKIELGQK
TGFLKFQSDFTRNSLKILLQCNCTRLKFEKRALKGKWLIMVNIVDNQPVV
NFITKWKRKNTNFRYSINVLRKFGNELCRLNLPNNSDGRTSMLKRYLAYA
KMNNITQFKNRKNRNNNSKENKKPKKSWTTQKKNAYKN*

>Pmo_009874 sfrp-1

MCILSLFFFNIFYLQIACFLGDWQQFQNGYSAEQCYKIPDNLTLCLNVGY
KRMILPNYIQHEYLEETVQHSKVWIGLVNSNCHEDIKKFLCSLYAPVCIY
NMKIQKVPPCKELCLSVKESCLPSMKLFGFEWPNIMECSLFPSGRNSLCI
PSTIHKDKKCRSCVKSASYEVMANKFCLSNIVIRGKIRRITAKKDNGYII
DLSQKSRFLKFQSNQSQSSLQIWLQCNCTKLKFENKALKGKWLIMVNIVK
GQPIVNFITKWKRRNIDFRNSINVLRKFGNELCRLNLPNNSDGRTSMLKR
YLAYAKMKNRNKASNKTK*

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